United States
Germany
Japan
United Kingdom
China
Cyp27a1 (BC002183) Mouse Tagged ORF Clone
CAT＃: MR206963
- TrueORF®
Cyp27a1 (Myc-DDK-tagged) - Mouse cytochrome P450, family 27, subfamily a, polypeptide 1 (cDNA clone MGC:7368 IMAGE:3487429)
ORF Plasmid: tGFP
"BC002183" in other vectors (3)
Need custom modification / cloning service?
Get a free quote
CNY 3,656.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 1300013A03Rik; Cyp27 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR206963 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTGGACAACCTCCTTTGGGACTTACACCAATGTGAATCTGGCTAGTGCCCCACTCTTGGAGCAAGTGA TGAGACAGGAGGGCAAGTACCCAATAAGAGACCACATGGATCAGTGGAAGGACCACCGAGACCACAAGGG CCTCACCTATGGGATCTTCATCGCACAAGGAGAGCAATGGTACCATCTGCGTCAGGCTTTGAAACAGAGG CTGCTGAAGCCTGACGAGGCCGCGCTCTACACGGATGCCTTAAACGAGGTTATCAGTGACTTTATCACCC GGCTGGACCAGGTGCGGGCAGAGAGTGAATCAGGGGACCAGGTGCCAGACATGGCTCATCTTCTCTACCA CCTTGCCTTGGAAGCCATCACCTATATCCTGTTTGAGAAAAGGATTGGCTGCCTGAAACCCTCCATTCCT GAGGACACTGCCGCCTTCATCAGATCTGTTGCAATCATGTTCCAGAACTCAGTCTATATCACTTTCCTTC CCAAATGGACGCGTCCTCTGCTGCCCTTTTGGAAGCGATACCTGAATGGCTGGGATAACATTTTCTCCTT TGGAAAGAAGCTGATTGATGAAAAAGTCCAGGAGCTAAAAGCCCAGCTACAGGAAACTGGGCCAGATGGA GTCCGGGTATCTGGCTACCTGCACTTCCTGCTGACCAATGAATTGCTCAGTACTCAGGAGACCATCGGCA CCTTTCCTGAGCTGCTTTTGGCTGGGGTGGACACGACATCCAACACACTGACATGGGCCCTGTACCACCT TTCAAAGAGCCCAGAGATCCAGGAGGCCTTGCACAAGGAAGTGACTGGTGTGGTGCCCTTCGGGAAGGTG CCCCAGCACAAGGACTTTGCCCACATGCCTCTGCTAAAAGCTGTGATTAAGGAGACCCTGCGCCTCTACC CTGTGGTTCCCACAAACTCCCGGATCATCACAGAAAAGGAAACTGAAATTAATGGCTTTCTCTTCCCCAA GAATACACAGTTTGTGTTATGCCACTACGTGGTGTCCCGGGATCCCAGTGTCTTTCCTGAGCCCAACAGC TTCCAGCCTCACCGATGGCTGAGGAAGAAAGAGGCTGATAACCCTGGGATCCTACATCCATTCGGCTCTG TGCCCTTCGGCTATGGGGTTCGGTCTTGCCTGGGTCGGAGGATTGCAGAACTGGAGATGCAACTGATGCT GTCAAGGCTGGTACAGAAGTATGAGATTGCCCTGGCTCCCGGGATGGGAGAAGTAAAGACTGTGTCCCGC ATCGTCCTGGTTCCCAGCAAGAAGGTGAGGCTGCATTTTCTGCAGAGACAG AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA >MR206963 protein sequence
Red=Cloning site Green=Tags(s) MWTTSFGTYTNVNLASAPLLEQVMRQEGKYPIRDHMDQWKDHRDHKGLTYGIFIAQGEQWYHLRQALKQR LLKPDEAALYTDALNEVISDFITRLDQVRAESESGDQVPDMAHLLYHLALEAITYILFEKRIGCLKPSIP EDTAAFIRSVAIMFQNSVYITFLPKWTRPLLPFWKRYLNGWDNIFSFGKKLIDEKVQELKAQLQETGPDG VRVSGYLHFLLTNELLSTQETIGTFPELLLAGVDTTSNTLTWALYHLSKSPEIQEALHKEVTGVVPFGKV PQHKDFAHMPLLKAVIKETLRLYPVVPTNSRIITEKETEINGFLFPKNTQFVLCHYVVSRDPSVFPEPNS FQPHRWLRKKEADNPGILHPFGSVPFGYGVRSCLGRRIAELEMQLMLSRLVQKYEIALAPGMGEVKTVSR IVLVPSKKVRLHFLQRQ SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC002183 |
| ORF Size | 1311 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC002183, AAH02183 |
| RefSeq Size | 1579 bp |
| RefSeq ORF | 1313 bp |
| Locus ID | 104086 |
| MW | 50.2 kDa |
| Gene Summary | Cytochrome P450 monooxygenase that catalyzes regio- and stereospecific hydroxylation of cholesterol and its derivatives. Hydroxylates (with R stereochemistry) the terminal methyl group of cholesterol side-chain in a three step reaction to yield at first a C26 alcohol, then a C26 aldehyde and finally a C26 acid. Regulates cholesterol homeostasis by catalyzing the conversion of excess cholesterol to bile acids via both the "neutral" (classic) and the "acid" (alternative) pathways. May also regulate cholesterol homeostasis via generation of active oxysterols, which act as ligands for NR1H2 and NR1H3 nuclear receptors, modulating the transcription of genes involved in lipid metabolism (By similarity). Plays a role in cholestanol metabolism in the cerebellum (PubMed:28190002). Similarly to cholesterol, hydroxylates cholestanol and may facilitate sterol diffusion through the blood-brain barrier to the systemic circulation for further degradation. Also hydroxylates retinal 7-ketocholesterol, a noxious oxysterol with pro-inflammatory and pro-apoptotic effects, and may play a role in its elimination from the retinal pigment epithelium. May play a redundant role in vitamin D biosynthesis. Catalyzes 25-hydroxylation of vitamin D3 that is required for its conversion to a functionally active form (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG206963 | Cyp27a1 (tGFP-tagged) - Mouse cytochrome P450, family 27, subfamily a, polypeptide 1 (cDNA clone MGC:7368 IMAGE:3487429) |
CNY 2,760.00 |
|
| MR206963L3 | Lenti ORF clone of Cyp27a1 (Myc-DDK-tagged) - Mouse cytochrome P450, family 27, subfamily a, polypeptide 1 (cDNA clone MGC:7368 IMAGE:3487429) |
CNY 4,370.00 |
|
| MR206963L4 | Lenti ORF clone of Cyp27a1 (mGFP-tagged) - Mouse cytochrome P450, family 27, subfamily a, polypeptide 1 (cDNA clone MGC:7368 IMAGE:3487429) |
CNY 4,370.00 |
其它Cyp27a1产品
