Etv6 (BC052163) Mouse Tagged ORF Clone

CAT#: MR207053

  • TrueORF®

Etv6 (Myc-DDK-tagged) - Mouse ets variant gene 6 (TEL oncogene) (cDNA clone MGC:59361 IMAGE:6513848)

ORF Plasmid: DDK tGFP



  "BC052163" in other vectors (4)

Need custom modification / cloning service?
Get a free quote

Mouse Protein / Lysate Request

CNY 5,488.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


ETV6 Antibody - middle region
    • 50 ug

CNY 4,628.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Synonyms AW123102; AW557856; Tel
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR207053 representing BC052163
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGGAAGACTCGATCCACCTGCCAACACACCTGCGTTTGCAGCCGATTTACTGGAGCAGAGATGACG
TAGCCCAGTGGCTCAAGTGGGCAGAAAATGAGTTTTCTTTAAGGCCCATTGAGAGCAACAAGTTCGAAAT
GAATGGCAAGGCCCTCCTGCTGCTGACCAAAGAGGATTTCCGCTACCGATCTCCTCATTCAGGCGACGTG
CTCTATGAACTCCTTCAGCATATCCTGAAGCAGAGGAAATCTCGAATGCTCTTCTCACCATTCTTCCCCC
CTGGGGACTCTATCCACACCAAGCCAGAGGTCCTCCTGCATCAGAACCATGACGAAGATAACTGTGTCCA
GAGGACACCCAGGACGCCCGCGGAGAGCGTGCACCACAACCCTCCCACCATCGAACTCTTACATCGCCCT
AGGTCACCCATCACCACAAACCACAGGCCTTCTCCTGACCCCGAACAGCAGCGGCCCCAGCGGTCCCCCC
TAGACAACATGAGCCGCCGCCTCTCGCCAGTGGAGAAAGCCCAGGGGCCCAGGCTACAGCAGGAGAACAA
CCACCAGGAAACGTACCCCCTGTCAGTGTCTCCTGTCGAGAATAATCACTGCCTGCCCTCAAGCCCCTGG
CAGGAGAGCACTCGAGTGATCCAGCTGATGCCCAGCCCCATCATGCACCCTTTGATCCTGAACCCCCGGC
ACTCGCACTCGGTGGACTTCAAACAGTCCCGGCACTCCGAGGATGGGATGAATCGGGAAGGGAAGCCCAT
CAACCTGTCTCATCGGGAGGACCTGGCTTACTTGAACCACATCATGGTCTCTATGTCCCCACCGGAAGAG
CACGCCATGCCCATTGGGAGAATAGCAGACTGTAGACTGCTTTGGGATTATGTCTATCAGTTGCTGTCTG
ACAGCCGGTACGAAAACTTCATCCGATGGGAGGACAAAGAATCCAAAATATTCCGGATAGTGGATCCCAA
CGGACTGGCTCGACTCTGGGGAAACCATAAGAACAGAACAAACATGACCTATGAGAAAATGTCCAGAGCC
CTGCGCCACTACTACAAACTAAACATTATCAGGAAGGAGCCCGGACAAAGGCTTTTGTTCAGGTTCATGA
AAACCCCAGATGAGATCATGAGTGGCCGGACAGACCGTCTAGAACACCTCGAGTCTCAAGTGCTGGATGA
ACAAACGTACCAAGAGGATGAACCTACCATAGCCTCACCGGTGGGCTGGCCAAGAGGAAACCTGCCCACG
GGGACCGCAGGAGGCGTGATGGAAGCAGGCGAGCTAGGGGTGGCTGTAAAGGAAGAGACCCGGGAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR207053 representing BC052163
Red=Cloning site Green=Tags(s)

MEEDSIHLPTHLRLQPIYWSRDDVAQWLKWAENEFSLRPIESNKFEMNGKALLLLTKEDFRYRSPHSGDV
LYELLQHILKQRKSRMLFSPFFPPGDSIHTKPEVLLHQNHDEDNCVQRTPRTPAESVHHNPPTIELLHRP
RSPITTNHRPSPDPEQQRPQRSPLDNMSRRLSPVEKAQGPRLQQENNHQETYPLSVSPVENNHCLPSSPW
QESTRVIQLMPSPIMHPLILNPRHSHSVDFKQSRHSEDGMNREGKPINLSHREDLAYLNHIMVSMSPPEE
HAMPIGRIADCRLLWDYVYQLLSDSRYENFIRWEDKESKIFRIVDPNGLARLWGNHKNRTNMTYEKMSRA
LRHYYKLNIIRKEPGQRLLFRFMKTPDEIMSGRTDRLEHLESQVLDEQTYQEDEPTIASPVGWPRGNLPT
GTAGGVMEAGELGVAVKEETRE

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC052163
ORF Size 1326 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC052163, AAH52163
RefSeq Size 2054 bp
RefSeq ORF 1328 bp
Locus ID 14011
MW 75.2 kDa
Gene Summary This gene encodes a transcriptional repressor belonging to the ETS family of proteins. Knockout of this gene in mice results in embryonic lethality due to defective angiogenesis. In humans, this gene is often involved in chromosome rearrangements associated with specific cancers. Alternate splicing of this gene results in multiple transcript variants. [provided by RefSeq, Dec 2014]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Other Versions

Customer Reviews 
Loading...