Tubb3 (NM_023279) Mouse Tagged ORF Clone
CAT#: MR207181
- TrueORF®
Tubb3 (Myc-DDK-tagged) - Mouse tubulin, beta 3 (Tubb3)
ORF Plasmid: tGFP
"NM_023279" in other vectors (4)
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CNY 3,656.00
CNY 3,990.00
Cited in 1 publication. |
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | 3200002H15Rik; M(beta)3; M(beta)6 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR207181 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGGGAGATCGTGCACATCCAGGCCGGCCAGTGCGGCAACCAGATAGGGGCCAAGTTCTGGGAGGTCA TCAGCGATGAGCACGGCATAGACCCCAGCGGCAACTATGTAGGGGACTCAGACCTGCAGCTGGAGCGCAT CAGCGTATACTACAATGAGGCCTCCTCTCACAAGTATGTGCCCAGGGCCATTCTGGTGGACTTGGAACCT GGAACCATGGACAGTGTTCGGTCTGGCGCCTTTGGACACCTATTCAGGCCCGACAACTTTATCTTTGGTC AGAGTGGTGCTGGCAACAACTGGGCCAAAGGGCACTATACGGAGGGCGCGGAGCTGGTGGACTCAGTCCT AGATGTCGTGCGGAAAGAGTGTGAGAATTGTGACTGCCTGCAGGGCTTCCAGCTGACACACTCACTGGGT GGGGGCACAGGCTCAGGCATGGGCACACTGCTCATCAGCAAGGTGCGTGAGGAGTACCCCGACCGCATCA TGAACACCTTCAGCGTGGTGCCTTCACCCAAAGTGTCGGACACTGTGGTGGAGCCCTACAACGCCACCCT GTCCATCCACCAGCTAGTGGAGAACACAGACGAGACCTACTGCATCGACAATGAAGCCCTCTACGACATC TGCTTCCGCACCCTCAAGCTGGCCACACCCACCTATGGGGACCTCAACCACCTTGTGTCTGCCACCATGA GTGGAGTCACCACCTCCCTTCGATTCCCTGGTCAGCTCAATGCCGACCTCCGCAAGCTGGCTGTGAACAT GGTGCCGTTCCCACGTCTCCACTTCTTCATGCCCGGCTTCGCCCCACTTACAGCCCGGGGCAGCCAGCAG TACCGTGCCCTGACGGTGCCTGAGCTCACGCAGCAGATGTTCGATGCCAAGAACATGATGGCTGCCTGTG ACCCGCGCCACGGTCGCTACCTGACCGTGGCCACTGTCTTCCGTGGGCGCATGTCTATGAAGGAGGTGGA CGAGCAGATGCTGGCCATCCAGAGTAAGAACAGCAGCTACTTCGTGGAGTGGATCCCCAACAACGTCAAG GTAGCCGTGTGTGACATCCCACCCCGTGGGCTCAAAATGTCATCCACCTTCATTGGCAACAGCACGGCCA TCCAGGAGCTGTTCAAACGCATCTCGGAGCAGTTCACAGCCATGTTCCGGCGCAAGGCCTTCCTGCACTG GTACACGGGCGAGGGCATGGATGAGATGGAGTTCACCGAGGCCGAGAGCAACATGAATGACCTGGTGTCC GAGTACCAGCAGTACCAGGACGCCACTGCGGAGGAGGAGGGGGAGATGTATGAAGATGATGACGAGGAAT CGGAAGCCCAGGGGCCCAAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR207181 protein sequence
Red=Cloning site Green=Tags(s) MREIVHIQAGQCGNQIGAKFWEVISDEHGIDPSGNYVGDSDLQLERISVYYNEASSHKYVPRAILVDLEP GTMDSVRSGAFGHLFRPDNFIFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKECENCDCLQGFQLTHSLG GGTGSGMGTLLISKVREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSIHQLVENTDETYCIDNEALYDI CFRTLKLATPTYGDLNHLVSATMSGVTTSLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTARGSQQ YRALTVPELTQQMFDAKNMMAACDPRHGRYLTVATVFRGRMSMKEVDEQMLAIQSKNSSYFVEWIPNNVK VAVCDIPPRGLKMSSTFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVS EYQQYQDATAEEEGEMYEDDDEESEAQGPK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_023279 |
ORF Size | 1353 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_023279.3 |
RefSeq Size | 1758 bp |
RefSeq ORF | 1353 bp |
Locus ID | 22152 |
UniProt ID | Q9ERD7 |
MW | 50.4 kDa |
Gene Summary | Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. TUBB3 plays a critical role in proper axon guidance and mantainance. Binding of NTN1/Netrin-1 to its receptor UNC5C might cause dissociation of UNC5C from polymerized TUBB3 in microtubules and thereby lead to increased microtubule dynamics and axon repulsion (PubMed:28483977). Plays a role in dorsal root ganglion axon projection towards the spinal cord (By similarity).[UniProtKB/Swiss-Prot Function] |
Citations (1)
The use of this cDNA Clones has been cited in the following citations: |
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iPSCs from a Hibernator Provide a Platform for Studying Cold Adaptation and Its Potential Medical Applications
,Ou, J;Ball, JM;Luan, Y;Zhao, T;Miyagishima, KJ;Xu, Y;Zhou, H;Chen, J;Merriman, DK;Xie, Z;Mallon, BS;Li, W;,
Cell
,PubMed ID 29576452
[TUBB3]
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Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC201693 | Tubb3 (untagged) - Mouse tubulin, beta 3 (Tubb3), (10ug) |
CNY 3,656.00 |
|
MG207181 | Tubb3 (tGFP-tagged) - Mouse tubulin, beta 3 (Tubb3) |
CNY 4,370.00 |
|
MR207181L3 | Lenti ORF clone of Tubb3 (Myc-DDK-tagged) - Mouse tubulin, beta 3 (Tubb3) |
CNY 5,890.00 |
|
MR207181L4 | Lenti ORF clone of Tubb3 (mGFP-tagged) - Mouse tubulin, beta 3 (Tubb3) |
CNY 5,890.00 |