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Chst2 (NM_018763) Mouse Tagged ORF Clone
CAT＃: MR207740
- TrueORF®
Chst2 (Myc-DDK-tagged) - Mouse carbohydrate sulfotransferase 2 (Chst2)
"NM_018763" in other vectors (3)
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CNY 3,952.00
CNY 4,660.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | AI428561; AW121776; C130041E03Rik; Chts2; GlcNAc6ST; Gn6st; GST-2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR207740 representing NM_018763
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCCGCAGCTCGCCGCGAGCTCTGCCCCCCGGTGCGCTTCCCCGGCCGCTGCCGGCCGCGCCTGCCG CCGTGCAGCGGGCCCTGCTCCCGCCGTGGCCCCGGCGCGCAGGACGCCGCTGGCCTGCGTCCCCGCTCGG GATGAAGGTATTTCGCAGGAAGGCGCTGGTGCTGTGCGCGGGCTATGCACTGCTACTGGTGCTCACGATG CTCAACCTCTTGGACTACAAGTGGCATAAAGAGCCGCTGCAGCAGTGCAACCCCGACGGGCCTCTGGGTG CCGCGGTAGGGGCGGCCGGGGCCGGCTGGGGACGGCCGGGGTCGCCTCCTGCAGCGCCACCCCGCGCTCA CTCTCGCATGGACCCCCGCACCCCGTACCGCCCTCCTGCCGCGGGCGTGGGGGCAGTTCCCGCAGCCGCG GCTGGGAGTGCAGGAGCTGCGGCCTCTCTGGGCAATGCTACTCGAGGCACCAGGGGTGGAGGGGACAAGC GGCAGTTGGTGTATGTGTTCACCACGTGGCGCTCGGGCTCGTCCTTCTTCGGTGAGCTCTTCAACCAGAA CCCTGAGGTGTTCTTCCTCTATGAGCCTGTGTGGCACGTGTGGCAAAAACTGTACCCCGGGGACGCCGTT TCCCTGCAGGGGGCAGCGCGGGACATGCTGAGCGCTCTCTACCGCTGCGATCTTTCGGTTTTCCAGCTGT ATAGCCCCGCAGGCAGTGGGGGGCGCAACCTCACCACTCTGGGCATCTTTGGGGCAGCCACTAACAAGGT GGTATGCTCCTCGCCACTCTGTCCTGCCTACCGCAAGGAGGTCGTCGGACTGGTGGACGACCGCGTGTGC AAAAAGTGCCCACCTCAACGCCTGGCACGCTTCGAGGAGGAGTGTCGCAAGTACCGCACGCTGGTTATCA AGGGCGTGCGGGTCTTCGATGTGGCTGTGTTGGCGCCGCTGCTTAAAGATCCAGCCTTGGACCTCAAGGT CATCCACCTAGTACGTGATCCTCGTGCTGTTGCCAGCTCCCGCATCCGCTCGCGTCACGGCCTCATCCGG GAAAGCCTACAGGTGGTGCGAAGCCGGGATCCAAGAGCCCACCGCATGCCCTTCCTGGAGGCTGCTGGCC ACAAGCTTGGTGCCAAGAAGGAGGGTATGGGTGGCCCAGCAGACTACCACGCTCTGGGTGCAATGGAGGT CATCTGCAACAGTATGGCCAAGACGCTGCAAACAGCCCTGCAGCCTCCTGACTGGCTGCAGGGACACTAC TTGGTGGTGAGGTACGAGGATCTGGTGGGAGACCCCGTTAAGACCCTACGGAGGGTATATGACTTTGTGG GGCTGCTGGTGAGTCCCGAAATGGAGCAGTTTGCCCTGAACATGACCAGTGGTTCGGGCTCCTCCTCCAA GCCTTTCGTGGTGTCAGCTCGCAATGCCACTCAGGCCGCCAATGCCTGGCGG[AT]CCGCGCTCACCTTC CAGCAGATCAAACAGGTGGAGGAGTTTTGCTACCAGCCCATGGCCGTGCTGGGCTATGAGCGGGTTAACA GTCCTGAGGAGGTCAAAGACCTCAGCAAGACCTTGCTCAGGAAGCCCCGGCTT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR207740 representing NM_018763
Red=Cloning site Green=Tags(s) MSRSSPRALPPGALPRPLPAAPAAVQRALLPPWPRRAGRRWPASPLGMKVFRRKALVLCAGYALLLVLTM LNLLDYKWHKEPLQQCNPDGPLGAAVGAAGAGWGRPGSPPAAPPRAHSRMDPRTPYRPPAAGVGAVPAAA AGSAGAAASLGNATRGTRGGGDKRQLVYVFTTWRSGSSFFGELFNQNPEVFFLYEPVWHVWQKLYPGDAV SLQGAARDMLSALYRCDLSVFQLYSPAGSGGRNLTTLGIFGAATNKVVCSSPLCPAYRKEVVGLVDDRVC KKCPPQRLARFEEECRKYRTLVIKGVRVFDVAVLAPLLKDPALDLKVIHLVRDPRAVASSRIRSRHGLIR ESLQVVRSRDPRAHRMPFLEAAGHKLGAKKEGMGGPADYHALGAMEVICNSMAKTLQTALQPPDWLQGHY LVVRYEDLVGDPVKTLRRVYDFVGLLVSPEMEQFALNMTSGSGSSSKPFVVSARNATQAANAWRXXALTF QQIKQVEEFCYQPMAVLGYERVNSPEEVKDLSKTLLRKPRL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_018763 |
| ORF Size | 1593 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_018763.2, NP_061233.2 |
| RefSeq Size | 6256 bp |
| RefSeq ORF | 1593 bp |
| Locus ID | 54371 |
| UniProt ID | Q80WV3 |
| MW | 58.3 kDa |
| Gene Summary | Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the transfer of sulfate to position 6 of non-reducing N-acetylglucosamine (GlcNAc) residues within keratan-like structures on N-linked glycans and within mucin-associated glycans that can ultimately serve as SELL ligands. SELL ligands are present in high endothelial cells (HEVs) and play a central role in lymphocyte homing at sites of inflammation. Participates in biosynthesis of the SELL ligand sialyl 6-sulfo Lewis X and in lymphocyte homing to Peyer patches. Has no activity toward O-linked sugars. Its substrate specificity may be influenced by its subcellular location. Sulfates GlcNAc residues at terminal, non-reducing ends of oligosaccharide chains.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC202009 | Chst2 (untagged) - Mouse carbohydrate sulfotransferase 2 (Chst2), (10ug) |
CNY 3,656.00 |
|
| MR207740L3 | Lenti ORF clone of Chst2 (Myc-DDK-tagged) - Mouse carbohydrate sulfotransferase 2 (Chst2) |
CNY 6,560.00 |
|
| MR207740L4 | Lenti ORF clone of Chst2 (mGFP-tagged) - Mouse carbohydrate sulfotransferase 2 (Chst2) |
CNY 6,560.00 |
