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Adsl (NM_009634) Mouse Tagged ORF Clone
CAT＃: MR207743
- TrueORF®
Adsl (Myc-DDK-tagged) - Mouse adenylosuccinate lyase (Adsl)
ORF Plasmid: tGFP
"NM_009634" in other vectors (4)
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CNY 3,330.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Adl; Asl |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR207743 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGCATCCGGCGACCCTGGCAGTGCTGAAAGTTACCGTTCTCCGCTGGCGGCCCGCTACGCCAGCC GCGAGATGTGTTTCTTGTTCAGCGACAGGTACAAGTTCCAGACGTGGCGGCAGCTGTGGCTGTGGCTGGC GGAGGCCGAACAGACGCTGGGTTTGCCTATCACAGATGAACAAATCCAGGAGATGAAGTCGAACCTGAAC AACATTGACTTCCAGATGGCAGCTGAGGAAGAGAAGCGCCTGCGGCACGACGTGATGGCTCACGTGCACA CGTTCGGCCACTGCTGTCCGAAAGCTGCGGGCATTATTCATCTGGGCGCCACCTCCTGCTATGTCGGAGA CAATACGGACCTGATTATTCTGAGAAATGCATTTGACCTACTTTTGCCAAAGCTTGCTAGAGTGATCTCG AGGCTTGCCGACTTTGCTAAGGATCGTGCTGATCTGCCCACGTTAGGTTTCACACACTTTCAGCCTGCTC AGCTGACCACGGTTGGGAAACGATGCTGCCTTTGGATTCAAGATCTCTGCATGGATCTCCAGAACTTGAA GCGTGTCCGGGATGAGCTGCGCTTCCGAGGAGTAAAGGGCACCACTGGCACGCAGGCCAGCTTCCTGCAG CTCTTCGAGGGGGATCACCAGAAGGTGGAGCAGCTGGACAAGATGGTGACAGAAAAGGCAGGGTTTAAAA GAGCCTTCATCATCACAGGACAGACGTACACACGGAAAGTGGACATCGAAGTGCTGTCTGTGCTGGCCAG CTTAGGAGCATCGGTGCACAAGATTTGCACTGACATACGCCTGCTGGCAAACCTGAAGGAGATGGAGGAG CCCTTTGAGAAACAGCAGATTGGCTCCAGTGCGATGCCGTACAAGCGGAACCCCATGCGCTCCGAACGTT GCTGCAGCCTGGCCCGTCACCTGATGGCCCTTACCATGGACCCACTACAGACAGCGTCTGTGCAGTGGTT TGAACGTACTCTGGATGACAGTGCCAACCGACGGATCTGTTTGGCTGAAGCGTTTCTCACTGCAGATACT ATATTAAACACCCTACAGAACATTTCTGAAGGATTGGTGGTGTACCCCAAAGTAATTGAACGGCGCATTC GGCAAGAGCTGCCTTTCATGGCCACAGAGAACATCATCATGGCAATGGTGAAAGCCGGGGGCAGCCGACA GGACTGCCATGAGAAAATTAGAGTGCTTTCCCAGCAGGCAGCTGCTGTGGTCAAGCAGGAAGGAGGTGAC AATGACCTTATAGAGCGCATCCGGGCAGATGCCTACTTCAGCCCCATCCACTCACAGCTGGAGCACTTGC TGGACCCCTCTTCTTTCACTGGCCGAGCACCCCAGCAGGTCCACAGATTCCTGGAAGAGGAAGTGCGCCC CCTGCTAAAGCCCTATGGGAATGAGATGGCGGTGAAAGCAGAGCTGTGTCTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR207743 protein sequence
Red=Cloning site Green=Tags(s) MAASGDPGSAESYRSPLAARYASREMCFLFSDRYKFQTWRQLWLWLAEAEQTLGLPITDEQIQEMKSNLN NIDFQMAAEEEKRLRHDVMAHVHTFGHCCPKAAGIIHLGATSCYVGDNTDLIILRNAFDLLLPKLARVIS RLADFAKDRADLPTLGFTHFQPAQLTTVGKRCCLWIQDLCMDLQNLKRVRDELRFRGVKGTTGTQASFLQ LFEGDHQKVEQLDKMVTEKAGFKRAFIITGQTYTRKVDIEVLSVLASLGASVHKICTDIRLLANLKEMEE PFEKQQIGSSAMPYKRNPMRSERCCSLARHLMALTMDPLQTASVQWFERTLDDSANRRICLAEAFLTADT ILNTLQNISEGLVVYPKVIERRIRQELPFMATENIIMAMVKAGGSRQDCHEKIRVLSQQAAAVVKQEGGD NDLIERIRADAYFSPIHSQLEHLLDPSSFTGRAPQQVHRFLEEEVRPLLKPYGNEMAVKAELCL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_009634 |
| ORF Size | 1455 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_009634.2 |
| RefSeq Size | 3712 bp |
| RefSeq ORF | 1455 bp |
| Locus ID | 11564 |
| UniProt ID | P54822 |
| MW | 54.9 kDa |
| Gene Summary | This gene encodes a protein that is involved in adenosine monophosphate (AMP) biosynthesis and maintaining AMP levels in the muscle. The encoded enzyme catalyzes the release of fumarate during AMP biosynthesis by cleaving the substrates succinylaminoimidazole carboxamide (SAICA) ribotide to give aminoimidazole carboxamide (AICA) ribotide, and adenylosuccinate to give adenylate. In humans, this gene is associated with adenylosuccinate deficiency, a rare autosomal disorder resulting in a spectrum of neurological symptoms. A pseudogene associated with this gene is located on the X chromosome. [provided by RefSeq, Jan 2013] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC201305 | Adsl (untagged) - Mouse adenylosuccinate lyase (Adsl), (10ug) |
CNY 2,000.00 |
|
| MG207743 | Adsl (tGFP-tagged) - Mouse adenylosuccinate lyase 1 (Adsl) |
CNY 3,710.00 |
|
| MR207743L3 | Lenti ORF clone of Adsl (Myc-DDK-tagged) - Mouse adenylosuccinate lyase (Adsl) |
CNY 5,230.00 |
|
| MR207743L4 | Lenti ORF clone of Adsl (mGFP-tagged) - Mouse adenylosuccinate lyase (Adsl) |
CNY 5,230.00 |
