Mlycd (NM_019966) Mouse Tagged ORF Clone
CAT#: MR207886
- TrueORF®
Mlycd (Myc-DDK-tagged) - Mouse malonyl-CoA decarboxylase (Mlycd), nuclear gene encoding mitochondrial protein
ORF Plasmid: tGFP
"NM_019966" in other vectors (4)
Need custom modification / cloning service?
Get a free quote
CNY 3,656.00
CNY 4,280.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | AI324784; Mcd |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR207886 representing NM_019966
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCGAGGCCTCGGGCCAGGCTTGAGGGCTCGGCGCCTGCTCCCACTGCGGTCCCCGCCGCGGCCTCCGG GGCCCCGGGGACGTCGGCTGTGCGGCGGGCTCGCGGCCAGCGCCATGGACGAGCTGCTGCGGCGAGCCGT GCCCCCGACGCCGGCCTACGAGCTGCGCGAGAAGACGCCGGCGCCCGCCGAGGGCCAGTGCGCCGACTTC GTGAGCTTCTACGGCGGCCTGGCCGAGGCGTCCCAGCGCGCCGAGCTGCTCGGCCGCCTGGCTCAGGGCT TCGGCGTGGACCACGGCCAGGTGGCCGAGCAGAGCGCCGGGGTGCTGCAGCTGCGCCAGCAGGCGCGCGA GGCGGCCGTGCTGCTGCAGGCGGAGGACCGGTTGCGCTACGCGCTCGTGCCGCGGTATCGCGGCCTCTTC CATCACATCAGCAAGCTGGACGGCGGCGTGCGCTTCTTGGTGCAGCTGCGGGCCGATCTGCTGGAGGCGC AGGCCCTCAAGCTGGTGGAGGGGCCGCACGTCCGGGAAATGAACGGAGTGCTCAAAAGCATGCTGTCTGA GTGGTTCTCCTCCGGCTTCCTGAACCTGGAGCGGGTCACCTGGCACTCACCCTGTGAGGTACTTCAGAAG ATCAGCGAGTGTGAGGCTGTGCACCCTGTGAAAAACTGGATGGACATGAAGCGGCGTGTGGGGCCCTACC GGAGATGTTACTTCTTCTCCCACTGCTCCACCCCCGGGGAACCCCTGGTGGTTCTGCATGTGGCTCTGAC TGGTGACATTTCCAACAACATCCAGGGCATTGTGAAGGAGTGCCCTCCGACTGAAACCGAGGAGAGGAAC AGGATCGCCGCTGCCATCTTCTACTCCATCAGCCTGACCCAGCAGGGCCTGCAGGGGGTGGAGCTCGGGA CCTTCCTCATAAAGAGAGTGGTCAAGGAGCTGCAGAAGGAATTTCCTCAGCTGGGGGCCTTTTCAAGCCT GTCGCCTATCCCTGGATTCACCAAGTGGCTGCTGGGCCTCCTGAATGTGCAGGGGAAGGAGCATGGGAGG AACGAACTATTCACAGACTCAGAGTGCCAAGAAATCTCAGCGGTTACCGGCAACCCTGTTCACGAGAGCC TCAAGGGGTTCCTGAGCAGTGGTGAGTGGGTGAAGTCAGAGAAGCTGACGCAGGCGCTGCAGGGGCCACT AATGAGGCTGTGTGCCTGGTACCTGTACGGTGAGAAGCACCGAGGCTACGCCCTCAACCCAGTGGCCAAC TTCCATCTGCAGAATGGGGCTGTGATGTGGCGTATCAACTGGATGGCTGACAGCAGCCTCAAAGGCCTCA CCAGCTCATGCGGCCTCATGGTCAACTACCGCTACTACCTGGAGGAGACAGGCCCCAACAGTATCTCCTA CCTGGGCTCCAAGAACATCAAAGCTTCCGAGCAGATCCTCAGCCTGGTAGCCCAGTTCCAGAACAACAGC AAACTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR207886 representing NM_019966
Red=Cloning site Green=Tags(s) MRGLGPGLRARRLLPLRSPPRPPGPRGRRLCGGLAASAMDELLRRAVPPTPAYELREKTPAPAEGQCADF VSFYGGLAEASQRAELLGRLAQGFGVDHGQVAEQSAGVLQLRQQAREAAVLLQAEDRLRYALVPRYRGLF HHISKLDGGVRFLVQLRADLLEAQALKLVEGPHVREMNGVLKSMLSEWFSSGFLNLERVTWHSPCEVLQK ISECEAVHPVKNWMDMKRRVGPYRRCYFFSHCSTPGEPLVVLHVALTGDISNNIQGIVKECPPTETEERN RIAAAIFYSISLTQQGLQGVELGTFLIKRVVKELQKEFPQLGAFSSLSPIPGFTKWLLGLLNVQGKEHGR NELFTDSECQEISAVTGNPVHESLKGFLSSGEWVKSEKLTQALQGPLMRLCAWYLYGEKHRGYALNPVAN FHLQNGAVMWRINWMADSSLKGLTSSCGLMVNYRYYLEETGPNSISYLGSKNIKASEQILSLVAQFQNNS KL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
![]() |
ACCN | NM_019966 |
ORF Size | 1476 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_019966.3 |
RefSeq Size | 2116 bp |
RefSeq ORF | 1479 bp |
Locus ID | 56690 |
UniProt ID | Q99J39 |
MW | 55.2 kDa |
Gene Summary | Catalyzes the conversion of malonyl-CoA to acetyl-CoA. In the fatty acid biosynthesis MCD selectively removes malonyl-CoA and thus assures that methyl-malonyl-CoA is the only chain elongating substrate for fatty acid synthase and that fatty acids with multiple methyl side chains are produced. In peroxisomes it may be involved in degrading intraperoxisomal malonyl-CoA, which is generated by the peroxisomal beta-oxidation of odd chain-length dicarboxylic fatty acids. Plays a role in the metabolic balance between glucose and lipid oxidation in muscle independent of alterations in insulin signaling. Plays a role in controlling the extent of ischemic injury by promoting glucose oxidation.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC200035 | Mlycd (untagged) - Mouse malonyl-CoA decarboxylase (Mlycd), nuclear gene encoding mitochondrial protein, (10ug) |
CNY 3,656.00 |
|
MG207886 | Mlycd (tGFP-tagged) - Mouse malonyl-CoA decarboxylase (Mlycd) |
CNY 5,256.00 |
|
MR207886L3 | Lenti ORF clone of Mlycd (Myc-DDK-tagged) - Mouse malonyl-CoA decarboxylase (Mlycd), nuclear gene encoding mitochondrial protein |
CNY 6,180.00 |
|
MR207886L4 | Lenti ORF clone of Mlycd (mGFP-tagged) - Mouse malonyl-CoA decarboxylase (Mlycd), nuclear gene encoding mitochondrial protein |
CNY 6,180.00 |