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Nfia (BC075702) Mouse Tagged ORF Clone
CAT＃: MR207901
- TrueORF®
Nfia (Myc-DDK-tagged) - Mouse nuclear factor I/A (cDNA clone MGC:76704 IMAGE:30087137)
ORF Plasmid: tGFP
"BC075702" in other vectors (4)
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CNY 3,656.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 1110047K16Rik; 9430022M17Rik; CTF; NF1-A; NF1A |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR207901 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTATTCTCCGCTCTGTCTCACCCAGGATGAGTTTCATCCTTTCATTGAAGCACTTCTGCCCCATGTCC GCGCCTTCGCCTACACATGGTTCAACCTGCAGGCCCGAAAGCGGAAATACTTCAAAAAACATGAGAAGCG CATGTCGAAAGAAGAGGAGAGGGCCGTGAAGGATGAACTGCTAAGCGAGAAGCCCGAGGTCAAGCAGAAG TGGGCTTCCCGACTTCTGGCCAAGTTACGGAAAGATATCCGACCCGAGTACCGAGAGGATTTTGTTCTTA CAGTTACAGGGAAAAAACCTCCATGCTGTGTTCTTTCCAACCCTGATCAGAAAGGCAAGATGCGGAGAAT TGACTGCCTCCGCCAGGCAGATAAAGTATGGAGGTTGGACCTCGTCATGGTGATCTTGTTCAAAGGTATT CCGCTGGAAAGTACTGATGGCGAGCGCCTTGTAAAGAGTCCACAGTGCTCTAATCCAGGGCTCTGTGTCC AGCCCCATCACATAGGGGTTTCTGTAAAGGAACTCGATTTATATTTGGCATACTTTGTACATGCAGCAGA TTCAAGTCAATCTGAAAGTCCCAGCCAGCCAAGTGAAGCTGACATTAAGGACCAGCCAGAAAATGGACAT TTGGGCTTCCAGGACAGCTTTGTCACATCAGGTGTTTTCAGTGTGACTGAGCTAGTAAGAGTGTCACAAA CACCAATAGCTGCAGGAACCGGCCCCAATTTTTCTCTCTCTGATTTGGAAAGTTCTTCATACTACAGCAT GAGTCCAGGAGCAATGAGGAGGTCTCTGCCCAGCACATCCTCTACCAGCTCTACAAAGCGCCTCAAGTCT GTGGAGGATGAAATGGACAGTCCTGGTGAAGAACCATTTTACACAGGCCAAGGGCGCTCCCCAGGGAGTG GCAGCCAGTCCAGTGGATGGCATGAAGTAGAGCCAGGCTTGCCATCTCCAAGCACACTGAAGAAGTCTGA GAAGTCTGGTTTCAGCAGCCCCTCCCCTTCGCAGACCTCCTCCCTGGGAACAGCATTCACACAGCATCAC CGACCTGTCATTACAGGACCCAGAGCAAGTCCACATGCGACGCCATCGACTCTCCACTTTCCAACGTCAC CCATCATCCAGCAGCCTGGGCCTTACTTCTCACACCCAGCCATCCGTTACCACCCTCAGGAGACGCTGAA AGAGTTTGTCCAACTTGTCTGTCCTGATGCTGGTCAGCAAGCTGGACAGGTGGGGTTCCTCAATCCCAAT GGAAGCAGTCAAGGCAAGGTGCACAACCCATTCCTCCCCACCCCAATGTTGCCGCCGCCGCCACCACCAC CGATGGCCAGACACCAAGCCTCCAACCACACCTCACCGACCTACTCGACACCCAGCACCTCCCCCGCAAA CCGATTCGTCAGTGTTGGACCACGGGATCCAAGCTTTGTAAATATCCCTCAACAGACACAGTCCTGGTAC CTGGGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR207901 protein sequence
Red=Cloning site Green=Tags(s) MYSPLCLTQDEFHPFIEALLPHVRAFAYTWFNLQARKRKYFKKHEKRMSKEEERAVKDELLSEKPEVKQK WASRLLAKLRKDIRPEYREDFVLTVTGKKPPCCVLSNPDQKGKMRRIDCLRQADKVWRLDLVMVILFKGI PLESTDGERLVKSPQCSNPGLCVQPHHIGVSVKELDLYLAYFVHAADSSQSESPSQPSEADIKDQPENGH LGFQDSFVTSGVFSVTELVRVSQTPIAAGTGPNFSLSDLESSSYYSMSPGAMRRSLPSTSSTSSTKRLKS VEDEMDSPGEEPFYTGQGRSPGSGSQSSGWHEVEPGLPSPSTLKKSEKSGFSSPSPSQTSSLGTAFTQHH RPVITGPRASPHATPSTLHFPTSPIIQQPGPYFSHPAIRYHPQETLKEFVQLVCPDAGQQAGQVGFLNPN GSSQGKVHNPFLPTPMLPPPPPPPMARHQASNHTSPTYSTPSTSPANRFVSVGPRDPSFVNIPQQTQSWY LG TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC075702 |
| ORF Size | 1476 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC075702, AAH75702 |
| RefSeq Size | 3001 bp |
| RefSeq ORF | 1478 bp |
| Locus ID | 18027 |
| MW | 54.4 kDa |
| Gene Summary | Recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC218207 | Nfia (untagged) - Mouse nuclear factor I/A (cDNA clone MGC:76704 IMAGE:30087137), (10ug) |
CNY 6,940.00 |
|
| MG207901 | Nfia (tGFP-tagged) - Mouse nuclear factor I/A (cDNA clone MGC:76704 IMAGE:30087137) |
CNY 3,140.00 |
|
| MR207901L3 | Lenti ORF clone of Nfia (Myc-DDK-tagged) - Mouse nuclear factor I/A (cDNA clone MGC:76704 IMAGE:30087137) |
CNY 4,750.00 |
|
| MR207901L4 | Lenti ORF clone of Nfia (mGFP-tagged) - Mouse nuclear factor I/A (cDNA clone MGC:76704 IMAGE:30087137) |
CNY 4,750.00 |
