Atp6v1b1 (NM_134157) Mouse Tagged ORF Clone
CAT#: MR208239
- TrueORF®
Atp6v1b1 (Myc-DDK-tagged) - Mouse ATPase, H+ transporting, lysosomal V1 subunit B1 (Atp6v1b1)
ORF Plasmid: tGFP
"NM_134157" in other vectors (4)
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CNY 3,832.00
CNY 4,560.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | Atp6b1; AW208839; D630003L15; D630030L16Rik; D630039P21Rik; Vpp-3; Vpp3 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR208239 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCACAACAGTAGACAGCAGGTCCTCGGGATTCACTGGCAATAGCTGTGACCCAGGCACAGCCCAAG AGCATGTGCAGGCTGTGACCCGAAACTACATCACCCACCCCCGAGTCACCTATAGGACAGTGTGCAGCGT CAATGGGCCTCTGGTGGTGCTGGACCAGGTCAAGTTTGCCCAGTATGCTGAGATTGTCAACTTTACCCTC CCTGATGGTACTCAGAGGAGTGGCCAGGTGCTTGAAGTGGCTGGGACCAAGGCCATTGTTCAGGTGTTTG AAGGGACTTCAGGGATTGATTCCCAGAAGACCACATGTGAATTCACAGGGGACATCCTGCGGACTCCTGT GTCAGAGGACATGCTGGGTCGGATTTTCAATGGCTCTGGCAAACCCATTGACAAAGGACCTGCTGTCATG GCAGAGGAGTTTCTGGACATCAACGGCCAGCCAATCAATCCCCATGACCGAATCTACCCCGAGGAGATGA TCCAGACGGGAATCTCCCCCATTGATGTCATGAACAGCATCGCTCGTGGCCAGAAGATCCCCATCTTCTC TGCGGCTGGGCTCCCCCACAATGAGATTGCAGCCCAGATCTGCCGCCAAGCCGGGCTGGTGAAGAAATCC AAGGCTGTGCTGGATTATCATGAAGACAACTTCGCCATTGTCTTTGCAGCCATGGGGGTGAACATGGAGA CAGCCAGGTTCTTCAAGTCAGATTTCGAGCAGAATGGAACCATGGGAAATGTCTGTCTCTTCCTGAATTT GGCCAATGACCCTACGATTGAGCGGATCATCACGCCACGTCTGGCACTGACCACGGCTGAGTTCCTTGCC TACCAGTGTGAGAAGCACGTGCTGGTGATACTGACTGACATGAGCTCCTATGCGGAGGCCTTGCGGGAGG TCTCAGCTGCCCGGGAGGAAGTGCCAGGACGCCGTGGCTTTCCTGGATATATGTACACAGACTTGGCCAC CATCTATGAGCGAGCTGGCCGTGTGGAGGGCCGGGGTGGTTCCATCACCCAGATCCCCATTCTCACCATG CCTAATGATGATATCACCCATCCCATCCCAGACTTGACTGGCTTCATCACAGAGGGGCAGATTTATGTGG ACCGACAGCTACATAACCGGCAGGTCTACCCACCCATCAATGTGCTCCCATCCCTCTCGCGACTGATGAA GTCGGCCATAGGCGAGGGCATGACCAGAAAGGACCACGGAGATGTTTCCAACCAGCTGTACGCCTGCTAC GCCATCGGGAAGGACGTGCAGGCCATGAAAGCGGTGGTGGGGGAGGAGGCGCTCACCTCCGAGGACCTGC TCTACCTGGAGTTCCTGCAGAAGTTTGAGAAGAATTTCATCACCCAAGGTCCTTACGAGAACCGCACGGT GTTCGAGTCGCTGGACCTGGGCTGGAAACTGCTGCGCATCTTTCCGAAAGAGATGCTGAAGCGCATTCCG CAGAGCATGACGGACGAGTTCTACTCTCGCCAGGGGGCGCAGCAGGACCCCGCATCCGACACGGCGCTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR208239 protein sequence
Red=Cloning site Green=Tags(s) MATTVDSRSSGFTGNSCDPGTAQEHVQAVTRNYITHPRVTYRTVCSVNGPLVVLDQVKFAQYAEIVNFTL PDGTQRSGQVLEVAGTKAIVQVFEGTSGIDSQKTTCEFTGDILRTPVSEDMLGRIFNGSGKPIDKGPAVM AEEFLDINGQPINPHDRIYPEEMIQTGISPIDVMNSIARGQKIPIFSAAGLPHNEIAAQICRQAGLVKKS KAVLDYHEDNFAIVFAAMGVNMETARFFKSDFEQNGTMGNVCLFLNLANDPTIERIITPRLALTTAEFLA YQCEKHVLVILTDMSSYAEALREVSAAREEVPGRRGFPGYMYTDLATIYERAGRVEGRGGSITQIPILTM PNDDITHPIPDLTGFITEGQIYVDRQLHNRQVYPPINVLPSLSRLMKSAIGEGMTRKDHGDVSNQLYACY AIGKDVQAMKAVVGEEALTSEDLLYLEFLQKFEKNFITQGPYENRTVFESLDLGWKLLRIFPKEMLKRIP QSMTDEFYSRQGAQQDPASDTAL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_134157 |
ORF Size | 1542 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_134157.1 |
RefSeq Size | 1945 bp |
RefSeq ORF | 1542 bp |
Locus ID | 110935 |
UniProt ID | Q91YH6 |
MW | 56.8 kDa |
Gene Summary | Non-catalytic subunit of the V1 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates protons (PubMed:16174750, PubMed:23028982). V-ATPase is responsible for acidifying and maintaining the pH of intracellular compartments and in some cell types, is targeted to the plasma membrane, where it is responsible for acidifying the extracellular environment (By similarity). Essential for the proper assembly and activity of V-ATPase (By similarity). In renal intercalated cells, mediates secretion of protons (H+) into the urine thereby ensuring correct urinary acidification (PubMed:16174750). Required for optimal olfactory function by mediating the acidification of the nasal olfactory epithelium (PubMed:23028982).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC203340 | Atp6v1b1 (untagged) - Mouse ATPase, H+ transporting, lysosomal V1 subunit B1 (Atp6v1b1), (10ug) |
CNY 3,824.00 |
|
MG208239 | Atp6v1b1 (tGFP-tagged) - Mouse ATPase, H+ transporting, lysosomal V1 subunit B1 (Atp6v1b1) |
CNY 5,040.00 |
|
MR208239L3 | Lenti ORF clone of Atp6v1b1 (Myc-DDK-tagged) - Mouse ATPase, H+ transporting, lysosomal V1 subunit B1 (Atp6v1b1) |
CNY 6,460.00 |
|
MR208239L4 | Lenti ORF clone of Atp6v1b1 (mGFP-tagged) - Mouse ATPase, H+ transporting, lysosomal V1 subunit B1 (Atp6v1b1) |
CNY 6,460.00 |
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