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Arfgap3 (NM_025445) Mouse Tagged ORF Clone
CAT＃: MR208404
- TrueORF®
Arfgap3 (Myc-DDK-tagged) - Mouse ADP-ribosylation factor GTPase activating protein 3 (Arfgap3)
ORF Plasmid: tGFP
"NM_025445" in other vectors (4)
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CNY 3,912.00
CNY 4,560.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 0610009H19Rik; 1810004P07Rik; 1810035F16Rik; 9130416J18Rik; Arfgap1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR208404 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGGACCCCAGCAAGCAGGACATCCTGGCCATCTTCAAGCGCCTGCGCTCGGTGCCCACTAACAAGG TGTGTTTTGACTGTGGTGCCAAAAATCCCAGCTGGGCCAGCATAAGCTATGGGGTGTTTCTCTGCATTGA CTGCTCTGGATCACACCGGTCGCTCGGGGTTCACTTGAGTTTTATTCGGTCCACAGAGCTGGATTCAAAC TGGTCGTGGTTCCAGCTTCGATGCATGCAAGTTGGAGGGAACTCAAATGCTTCTTCCTTCTTCCACCAAC ATGGGTGTGCCACCAAGGACACCAACGCCAAGTACAACAGCCGTGCGGCTCAGCTCTACAGGGAGAAGAT CAAGACTCTGGCCACCCAGGCCACCCGCAGGCACGGCACCGATCTGTGGCTGGACAGCTGTGCGGCTCCA CCTGTGTCCCCTCCTCCAAAGGAGGAAGATTTTTTTGCTTCTCACGCCTCTCTGGAGGTCAGTGGTGCTA TGCAGGCATCCGCACAGCCAGAGTCAGCCTCTTCAACACCGTGGGGCTTGGAAACCACTCCAGAAAAACA TGAAGGTGGGCCAGGACAAGGGCCAAGCGTGGAAGGTCTGAACACCCCTGGAAAGGCTGCACCAGAGGTT TCATCGATTATAAAAAAGAAGCCAAATCAAGCAAAAAAAGGACTTGGAGCCAAAAAAGGAAGCTTGGGAG CCCAGAAACTGACAAATACATCCTTCACTGAGATCGAAAAGCAAGCTCAGGCTGTGGATAAGAGGAAGGA GCAGGAAGACCTTGCCAGGGGTGCCCCAAAGGAGGAGTCTATTGTTTCGTCCTTGCGACTGGCCTACAAG GATCTGGAAATCAGCAGGAAGAAAGATGAAAGACTGAACCTGAGTGGCCAGAAGAAGGTGGAAGCCGAGC GGCTGGGCATGGGCTTTGGAAGCTGCAGGAGTGGCATCTCCCACTCGGTGACTTCAGACATGCAGACCAT TGAGCAGGAGTCGCCCACACTGGCAAAGCCGAGGAGGAAGTACCAGGAGGACCCAGAGGACTCGTACTTC AGCTCCAGCTCCAAGTGGTCTGAGCAGAGCAGCAGCAGGTACTTTGACGACCCCATGGAGTTAAGAAGCA GCTCCTTCTCTAGCTGGGATGATGGTGCCGATTCCTACTGGAAGAAAGACAGCAGCAGAGACCCCGAGCC TGCCATGAGAAGCACCGGCTCTTCAGACAGGCCCAGTGCTCGCCGGAAGCCAGAATATGAGCCCATTGGC AGCACAGATGAAGCCCAGAAGAAGTTTGGCAACGTCAAGGCCATCTCTTCAGACATGTACTTTGGAATTC AAGCCCAGACAGATTTTGAAACCCGGGCGCGGCTGGAGAGGCTCTCCACCAGCTCCTCCATAAGCTCTGC AGACCTTTTTGATGAGCAGAGGAAGCAGACTGCAGGAAACTACAACCTTTCCAATGTGCTGCCCAATGCC CCAGACATGGCGCAGTTCAAGCAAGGTGTGCGCTCCGTTGCTGGAAAACTTTCCGTCTTCGCCAATGGGG TCATGACGTCCATTCAGGATCGCTACGGTTCT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR208404 protein sequence
Red=Cloning site Green=Tags(s) MGDPSKQDILAIFKRLRSVPTNKVCFDCGAKNPSWASISYGVFLCIDCSGSHRSLGVHLSFIRSTELDSN WSWFQLRCMQVGGNSNASSFFHQHGCATKDTNAKYNSRAAQLYREKIKTLATQATRRHGTDLWLDSCAAP PVSPPPKEEDFFASHASLEVSGAMQASAQPESASSTPWGLETTPEKHEGGPGQGPSVEGLNTPGKAAPEV SSIIKKKPNQAKKGLGAKKGSLGAQKLTNTSFTEIEKQAQAVDKRKEQEDLARGAPKEESIVSSLRLAYK DLEISRKKDERLNLSGQKKVEAERLGMGFGSCRSGISHSVTSDMQTIEQESPTLAKPRRKYQEDPEDSYF SSSSKWSEQSSSRYFDDPMELRSSSFSSWDDGADSYWKKDSSRDPEPAMRSTGSSDRPSARRKPEYEPIG STDEAQKKFGNVKAISSDMYFGIQAQTDFETRARLERLSTSSSISSADLFDEQRKQTAGNYNLSNVLPNA PDMAQFKQGVRSVAGKLSVFANGVMTSIQDRYGS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_025445 |
| ORF Size | 1575 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_025445.1, NM_025445.2, NM_025445.3, NM_025445.4, NP_079721.2 |
| RefSeq Size | 2618 bp |
| RefSeq ORF | 1575 bp |
| Locus ID | 66251 |
| UniProt ID | Q9D8S3 |
| MW | 57.5 kDa |
| Gene Summary | GTPase-activating protein (GAP) for ADP ribosylation factor 1 (ARF1). Hydrolysis of ARF1-bound GTP may lead to dissociation of coatomer from Golgi-derived membranes to allow fusion with target membranes (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC200353 | Arfgap3 (untagged) - Mouse ADP-ribosylation factor GTPase activating protein 3 (Arfgap3), (10ug) |
CNY 3,912.00 |
|
| MG208404 | Arfgap3 (tGFP-tagged) - Mouse ADP-ribosylation factor GTPase activating protein 3 (Arfgap3) |
CNY 5,040.00 |
|
| MR208404L3 | Lenti ORF clone of Arfgap3 (Myc-DDK-tagged) - Mouse ADP-ribosylation factor GTPase activating protein 3 (Arfgap3) |
CNY 6,460.00 |
|
| MR208404L4 | Lenti ORF clone of Arfgap3 (mGFP-tagged) - Mouse ADP-ribosylation factor GTPase activating protein 3 (Arfgap3) |
CNY 6,460.00 |
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