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Hrg (NM_053176) Mouse Tagged ORF Clone
CAT＃: MR208427
- TrueORF®
Hrg (Myc-DDK-tagged) - Mouse histidine-rich glycoprotein (Hrg)
ORF Plasmid: tGFP
"NM_053176" in other vectors (3)
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CNY 3,920.00
CNY 4,660.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | AI265597; AW413091; D16jh2; D18020; Hprg; Hrgp |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR208427 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGGTGCTCACCACAGCATTGCTTCTAGTCACCCTGCAGTGTTCCCATGCCCTGAGTCCCACCAACT GTGATGCTTCTGAGCCTCTGGCTGAGAAAGTTCTAGACCTGATCAATAAAGGGCGGAGGAGTGGCTATGT TTTCGAGCTGCTGCGGGTCTCTGACGCCCACTTGGACAGAGCGGGAACGGCCACAGTCTACTACTTAGCT TTAGATGTGATAGAGTCTGACTGTTGGGTCCTTTCCACAAAAGCCCAGGATGACTGTCTTCCATCGAGGT GGCAATCTGAAATAGTGATTGGACAATGTAAGGTAATAGCCACAAGATACTCAAATGAGTCTCAAGATCT TAGCGTGAATGGCTATAACTGCACCACAAGTTCTGTCTCTTCAGCACTTCGCAATACCAAGGACAGTCCT GTACTCTTGGACTTCTTTGAGGATTCCGAGCTCTACAGAAAGCAAGCCCGTAAAGCCCTGGACAAGTACA AAACGGATAATGGTGACTTTGCCTCTTTCAGAGTGGAACGGGCAGAACGGGTTATAAGAGCGAGAGGAGG GGAAAGAACCAATTACTATGTGGAATTCTCCATGAGGAACTGTTCCACACAGCATTTCCCCAGGTCCCCT CTGGTCTTTGGATTCTGCAGAGCACTTTTGTCCTACAGTATAGAAACCTCTGACTTGGAAACCCCAGATT CCATTGATATAAACTGCGAAGTCTTCAACATTGAGGATCATAAAGACACAAGTGACATGAAACCCCATTG GGGCCATGAGCGTCCTCTTTGTGACAAACATCTGTGTAAGCTCAGTGGATCCAGGGATCATCATCATACC CATAAGACAGATAAACTTGGATGCCCACCTCCACCAGAAGGAAAAGATAACTCAGACAGACCACGCCTTC AAGAAGGAGCCCTTCCACAACTGCCCCCTGGCTATCCCCCTCATTCTGGTGCCAACAGAACCCATAGACC CTCTTATAATCACAGTTGTAATGAGCATCCTTGTCATGGACATCGTCCCCATGGACACCACCCCCACAGT CACCATCCCCCCGGTCACCATTCCCATGGTCACCATCCCCACGGTCACCATCCCCACAGTCACCATTCCC ACGGTCACCATCCCCCCGGTCACCATCCCCATGGTCACCACCCCCACGGTCACCATCCCCATGGTCACCA TCCCCACGGTCACCATCCTCATGGACATGACTTCCTTGACTATGGACCTTGTGACCCGCCCTCCAATAGT CAAGAACTCAAAGGTCAGTATCATCGGGGCTATGGTCCACCACATGGACACTCAAGAAAAAGAGGTCCAG GCAAAGGACTCTTTCCTTTCCACCACCAACAAATTGGATATGTCTACCGACTCCCTCCACTGAATATAGG TGAAGTTCTCACTCTTCCTGAAGCCAATTTCCCCAGCTTCTCTTTGCCAAATTGCAACAGATCCCTACAA CCAGAGATTCAGCCCTTCCCTCAGACAGCCTCCAGGTCATGTCCAGGGAAATTTGAGAGTGAATTTCCAC AAATTTCCAAGTTTTTTGGATATACACCTCCAAAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR208427 protein sequence
Red=Cloning site Green=Tags(s) MKVLTTALLLVTLQCSHALSPTNCDASEPLAEKVLDLINKGRRSGYVFELLRVSDAHLDRAGTATVYYLA LDVIESDCWVLSTKAQDDCLPSRWQSEIVIGQCKVIATRYSNESQDLSVNGYNCTTSSVSSALRNTKDSP VLLDFFEDSELYRKQARKALDKYKTDNGDFASFRVERAERVIRARGGERTNYYVEFSMRNCSTQHFPRSP LVFGFCRALLSYSIETSDLETPDSIDINCEVFNIEDHKDTSDMKPHWGHERPLCDKHLCKLSGSRDHHHT HKTDKLGCPPPPEGKDNSDRPRLQEGALPQLPPGYPPHSGANRTHRPSYNHSCNEHPCHGHRPHGHHPHS HHPPGHHSHGHHPHGHHPHSHHSHGHHPPGHHPHGHHPHGHHPHGHHPHGHHPHGHDFLDYGPCDPPSNS QELKGQYHRGYGPPHGHSRKRGPGKGLFPFHHQQIGYVYRLPPLNIGEVLTLPEANFPSFSLPNCNRSLQ PEIQPFPQTASRSCPGKFESEFPQISKFFGYTPPK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_053176 |
| ORF Size | 1578 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_053176.2 |
| RefSeq Size | 1758 bp |
| RefSeq ORF | 1611 bp |
| Locus ID | 94175 |
| UniProt ID | Q9ESB3 |
| MW | 59.2 kDa |
| Gene Summary | Plasma glycoprotein that binds a number of ligands such as heme, heparin, heparan sulfate, thrombospondin, plasminogen, and divalent metal ions. Binds heparin and heparin/glycosaminoglycans in a zinc-dependent manner. Binds heparan sulfate on the surface of liver, lung, kidney and heart endothelial cells. Binds to N-sulfated polysaccharide chains on the surface of liver endothelial cells. Inhibits rosette formation. Acts as an adapter protein and is implicated in regulating many processes such as immune complex and pathogen clearance, cell chemotaxis, cell adhesion, angiogenesis, coagulation and fibrinolysis. Mediates clearance of necrotic cells through enhancing the phagocytosis of necrotic cells in a heparan sulfate-dependent pathway. This process can be regulated by the presence of certain HRG ligands such as heparin and zinc ions. Binds to IgG subclasses of immunoglobins containing kappa and lambda light chains with different affinities regulating their clearance and inhibiting the formation of insoluble immune complexes. Tethers plasminogen to the cell surface. Binds T-cells and alters the cell morphology. Acts as a regulator of the vascular endothelial growth factor (VEGF) signaling pathway; inhibits endothelial cell motility by reducing VEGF-induced complex formation between PXN/paxillin and ILK/integrin-linked protein kinase and by promoting inhibition of VEGF-induced tyrosine phosphorylation of focal adhesion kinases and alpha-actinins in endothelial cells. Also plays a role in the regulation of tumor angiogenesis and tumor immune surveillance. Normalizes tumor vessels and promotes antitumor immunity by polarizing tumor-associated macrophages, leading to decreased tumor growth and metastasis (By similarity). Modulates angiogenesis by blocking the CD6-mediated antiangiongenic effect of thrombospondins, THBS1 and THBS2.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG208427 | Hrg (tGFP-tagged) - Mouse histidine-rich glycoprotein (Hrg) |
CNY 5,130.00 |
|
| MR208427L3 | Lenti ORF clone of Hrg (Myc-DDK-tagged) - Mouse histidine-rich glycoprotein (Hrg) |
CNY 6,560.00 |
|
| MR208427L4 | Lenti ORF clone of Hrg (mGFP-tagged) - Mouse histidine-rich glycoprotein (Hrg) |
CNY 6,560.00 |
