Cnot2 (BC063105) Mouse Tagged ORF Clone

CAT#: MR208509

  • TrueORF®

Cnot2 (Myc-DDK-tagged) - Mouse CCR4-NOT transcription complex, subunit 2 (cDNA clone MGC:70296 IMAGE:5003026)

ORF Plasmid: DDK tGFP



  "BC063105" in other vectors (4)

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Mouse Protein / Lysate Request

CNY 3,952.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Synonyms 2600016M12Rik; 2810470K03Rik; AA537049; AA959607; AW557563; C79650
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR208509 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTTGAAAGAAGTTGCACAGGTGACAAACAGCATGTTTGGTGCTTCAAGAAAGAAGTTTGTAGAGGGGG
TGGACAGCGACTACCATGATGAGAACATGTACTACAGCCAGTCTTCTATGTTCCCACATCGGTCAGAGAA
AGATATGCTGGCATCGCCATCTACGTCAGGTCAGCTGTCTCAATTTGGGGCAAGTTTATACGGGCAACAA
AGTGCACTAGGCCTTCCAATGAGGGGGATGAGCAACAATACCCCTCAGTTAAATCGCAGCTTATCACAAG
GCACTCAGTTACCGAGCCACGTCACGCCAACAACAGGGGTACCAACAATGTCACTTCACACGCCTCCATC
TCCAAGCAGGGGTATTTTGCCTATGAATCCTAGGAATATGATGAACCACTCCCAGGTTGGTCAGGGCATT
GGAATTCCTAGCAGGACAAATAGCATGAGCAGTTCAGGGTTAGGTAGCCCCAACAGAAGCTCGCCAAGCA
TAATATGTATGCCAAAGCAACAGCCTTCTCGACAGCCTTTTACTGTGAACAGTATGTCTGGATTTGGAAT
GAACAGGAATCAGGCATTTGGAATGAATAACTCCTTATCAAGTAACATTTTTAATGGAACAGATGGCAGT
GAAAATGTGACAGGATTGGACCTTTCAGATTTCCCAGCGTTAGCTGACCGGAATAGAAGGGAAGGGAGCG
GCAACCCAACTCCATTAATAAACCCCTTGGCTGGAAGAGCTCCTTACGTTGGAATGGTAACAAAACCTGC
AAATGAGCAATCCCAAGACTTCTCAATACACAACGAAGATTTTCCAGCATTACCTGGTTCCAGCTATAAA
GATCCAACGTCAAGTAATGACGACAGCAAATCTAATTTGAGTACATCGGGGAAGACGACTTCAAGTACAG
ATGGACCCAAATTCCCTGGAGATAAAAGTTCAACAACACAAAACAATAATCAACAGAAAAAGGGGATCCA
GGTGTTACCTGATGGTCGAGTTACTAACATTCCTCAAGGGATGGTGACGGACCAATTTGGAATGATTGGC
CTGTTAACATTTATCAGGGCAGCAGAGACAGACCCAGGAATGGTACATCTTGCTTTAGGAAGTGACTTAA
CAACATTAGGCCTCAATCTGAACTCACCTGAAAATCTCTATCCCAAATTTGCATCACCCTGGGCTTCTTC
ACCTTGTCGGCCTCAAGACATAGACTTCCATGTTCCATCAGAATATTTAACAAACATTCACATTAGGGAT
AAGCTGGCTGCAATCAAACTTGGCCGATATGGAGAAGACCTCCTCTTCTATCTGTATTACATGAACGGCG
GGGATGTATTACAACTCTTAGCTGCGGTAGAACTTTTTAACCGTGATTGGAGATACCACAAGGAGGAGCG
GGTATGGATTACCAGGGCACCAGGCATGGAGCCAACAATGAAAACCAACACGTATGAGCGGGGGACCTAC
TATTTCTTTGACTGTCTCAACTGGAGGAAAGTAGCTAAGGAGTTCCATCTGGAATATGACAAATTAGAAG
AGCGGCCTCACCTGCCATCCACCTTCAACTACAACCCTGCTCAGCAAGCCTTC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR208509 protein sequence
Red=Cloning site Green=Tags(s)

MLKEVAQVTNSMFGASRKKFVEGVDSDYHDENMYYSQSSMFPHRSEKDMLASPSTSGQLSQFGASLYGQQ
SALGLPMRGMSNNTPQLNRSLSQGTQLPSHVTPTTGVPTMSLHTPPSPSRGILPMNPRNMMNHSQVGQGI
GIPSRTNSMSSSGLGSPNRSSPSIICMPKQQPSRQPFTVNSMSGFGMNRNQAFGMNNSLSSNIFNGTDGS
ENVTGLDLSDFPALADRNRREGSGNPTPLINPLAGRAPYVGMVTKPANEQSQDFSIHNEDFPALPGSSYK
DPTSSNDDSKSNLSTSGKTTSSTDGPKFPGDKSSTTQNNNQQKKGIQVLPDGRVTNIPQGMVTDQFGMIG
LLTFIRAAETDPGMVHLALGSDLTTLGLNLNSPENLYPKFASPWASSPCRPQDIDFHVPSEYLTNIHIRD
KLAAIKLGRYGEDLLFYLYYMNGGDVLQLLAAVELFNRDWRYHKEERVWITRAPGMEPTMKTNTYERGTY
YFFDCLNWRKVAKEFHLEYDKLEERPHLPSTFNYNPAQQAF

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC063105
ORF Size 1593 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC063105, AAH63105
RefSeq Size 3014 bp
RefSeq ORF 1595 bp
Locus ID 72068
MW 58.6 kDa
Gene Summary Component of the CCR4-NOT complex which is one of the major cellular mRNA deadenylases and is linked to various cellular processes including bulk mRNA degradation, miRNA-mediated repression, translational repression during translational initiation and general transcription regulation. Additional complex functions may be a consequence of its influence on mRNA expression. Required for the CCR4-NOT complex structural integrity. Can repress transcription and may link the CCR4-NOT complex to transcriptional regulation; the repressive function may specifically involve the N-Cor repressor complex containing HDAC3, NCOR1 and NCOR2. Involved in the maintenance of embryonic stem (ES) cell identity; prevents their differentiation towards extraembryonic trophectoderm lineages.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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