Zfp451 (BC019546) Mouse Tagged ORF Clone

CAT#: MR208860

  • TrueORF®

Zfp451 (Myc-DDK-tagged) - Mouse zinc finger protein 451 (cDNA clone MGC:28438 IMAGE:4038791)

ORF Plasmid: DDK tGFP



  "BC019546" in other vectors (4)

Need custom modification / cloning service?
Get a free quote

Mouse Protein / Lysate Request

CNY 4,160.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Synonyms 4930515K21Rik; 4933435G09Rik; AI596398; COASTER; Kiaa0576-hp; mKIAA1702; Znf451
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR208860 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGAGACCCAGGGCCGGAGATAATAGAATCTGTCCCACCAGCTGGGCCCGAGGCATCAGAGTCTACCA
CGGATGAAAACGAAGATGACATTCAGTTTGTTAGTGAAGGACCGTTAAGACCCGTTTTGGAATACATTGA
TCTAGTCAGCAGTGATGATGAAGAGCCTAGTACCTCTCACAGTGATAGAATGCCTGAATCTAAAGTGCCA
TCCTCTGAGAATCATCGCCCAGAAATGTGCTCTAGCTGCAGTGTTCCTCTTCCCATTGGAGACAGCAGCT
CCTCCTCTGGGAGTTGTGCCAACAGCCCACAAAGGATAGTTTCTCAACCTTCTTCTGTTGAGAACCCATT
GGAGAACCAGAAAAATGATCACAATAATTCCGATGTTAGTGTCTCTGAAAAAGAGACACTTAAACCACAA
AGTAATCAGACTCTGCCTTCAGCATCACTTCTGGGCTCCCAGGAATCTTTGGCTTTTTCTGAGGTCAAAG
AGGATTTACCTGGCACGATGTCTTCAGCTTCACAGAGTGGACAGGATGCCATCCTGTATCTCCAGACACA
AGTGGCTGAGATGTCCCGAGTCATACGTGATCTGCAGTCCAGGAGTTGTTTTAGATTTCATCATTCTAGG
CCAAGTGAGAGCTCGTCAGTTCCTTGGGATATCTCCAGCTCTAAGGAGGACAGTTTATCCACAGTTGAAG
AGGAGTCTGACTGCAAATCTCCGTCAGCTGATGACAAAGGGCAGCCACCTGACCCCATGCAGTCTAGTTT
TACCGGTCTTTTGAAGAGAATGGAACAAAGGGGTGTTATAAAGAGGGTGACATTCCAGTCTGAAGCAGAA
ACCTGTGAGGGCAAGCCTGATTGGATGACCTCTAAAAAACGCTTGGTTCCTCCACTGCATCCACTTTTGA
GAATTGCCACCACTGAAGTTTTTAAAGACCCTGCTGATTGCCATCCTTCTTCCTTCATGGGACACAGGGT
TTATCCTGTGGCCAAGGACACATCTCCTTTCCAGCCAAATCCACCAGCTGAGGGACCCATCATAGATGTA
TTAGAACATAGCAAAAGAGGAAACACAGCATCGCCTTTAGACTCTACCTCAAAAGAAATGGAGGTCATGG
GTTGCAGGTTCTACCATGCTGCTTCCATTGCAGCCCGGGCTGCTAGCTACATGGCCTATATGACTCAGTA
TCAACGTAAACTCTGGGAAGACATGGAAGATCTGGTTCATGACCCGGAGTTTGATCGTGGGAAAGCAAGG
TGTATAATATCTGATGGCATGGATGCAGGACTTTGGCAGCTCTGTACCACTAGGGACATAATGGACTCTG
TAGTCAGAGTTATGGCCATGGCAGTAGACTATAGAAGACAGGCCTGGCTCCGACTAACATCTCTCACTAA
GAAAACCCAGGAGAAGATCTTACACTTGCCCTTTGATGGTACATCTCTTTTTGGACAAGATGTGAATGCT
ATTGTTGCAGAAGACAATAGTATTAAAGAAAATGAATATAAGGATCACAATAAATACTATACTCAGCATC
GGTACTTTTATAGTCATGATCAGAAAGCACATTATCACAACAGAGGGTACTCCAAAGGGGATTGGTACAA
ACCCCGGAATCACCCCTATAGATACAGAAAAAAGGGAGACTCTCCAGAACGGCATGGGTATAAGAAC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR208860 protein sequence
Red=Cloning site Green=Tags(s)

MGDPGPEIIESVPPAGPEASESTTDENEDDIQFVSEGPLRPVLEYIDLVSSDDEEPSTSHSDRMPESKVP
SSENHRPEMCSSCSVPLPIGDSSSSSGSCANSPQRIVSQPSSVENPLENQKNDHNNSDVSVSEKETLKPQ
SNQTLPSASLLGSQESLAFSEVKEDLPGTMSSASQSGQDAILYLQTQVAEMSRVIRDLQSRSCFRFHHSR
PSESSSVPWDISSSKEDSLSTVEEESDCKSPSADDKGQPPDPMQSSFTGLLKRMEQRGVIKRVTFQSEAE
TCEGKPDWMTSKKRLVPPLHPLLRIATTEVFKDPADCHPSSFMGHRVYPVAKDTSPFQPNPPAEGPIIDV
LEHSKRGNTASPLDSTSKEMEVMGCRFYHAASIAARAASYMAYMTQYQRKLWEDMEDLVHDPEFDRGKAR
CIISDGMDAGLWQLCTTRDIMDSVVRVMAMAVDYRRQAWLRLTSLTKKTQEKILHLPFDGTSLFGQDVNA
IVAEDNSIKENEYKDHNKYYTQHRYFYSHDQKAHYHNRGYSKGDWYKPRNHPYRYRKKGDSPERHGYKN

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC019546
ORF Size 1677 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC019546, AAH19546
RefSeq Size 1928 bp
RefSeq ORF 1679 bp
Locus ID 98403
MW 62.7 kDa
Gene Summary E3 SUMO-protein ligase; has a preference for SUMO2 and SUMO3 and facilitates UBE2I/UBC9-mediated sumoylation of target proteins. Plays a role in protein SUMO2 modification in response to stress caused by DNA damage and by proteasome inhibitors (in vitro). Required for MCM4 sumoylation. Has no activity with SUMO1 (PubMed:26524493). Preferentially transfers an additional SUMO2 chain onto the SUMO2 consensus site 'Lys-11'. Negatively regulates transcriptional activation mediated by the SMAD4 complex in response to TGF-beta signaling. Inhibits EP300-mediated acetylation of histone H3 at 'Lys-9'. Plays a role in regulating the transcription of AR targets (By similarity).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Other Versions

Customer Reviews 
Loading...