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C8b (NM_133882) Mouse Tagged ORF Clone
CAT＃: MR209183
- TrueORF®
C8b (Myc-DDK-tagged) - Mouse complement component 8, beta polypeptide (C8b)
ORF Plasmid: tGFP
"NM_133882" in other vectors (4)
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CNY 4,392.00
CNY 4,560.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 4930439B20Rik; AI595927 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR209183 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGATAGGGGCTCAGGTTTGGAGGGCACTGGCAAAGTCGTGTCTCCTCTGTGCTACTCTGGGATGTC TCCATTTCCCTGGTTCCAGAGGTGGGAAGCCCGATTTCTTTGAGACAAAGGCAGTCAATGGGAGCCTTGT CAAGAGCAGACCAGTACGGAGTGTGGCGGAAGCCCCAGCACCTATTGACTGTGAGCTATCCACCTGGTCT TCCTGGACTGCATGTGACCCCTGTCAGAAGAAAAGGTACAGACATACTTACTTGCTCCGGCCCTCTCAGT TCTATGGGGAATTGTGTGACTTGTCCGACAAGGAAGTTGAAGACTGTGTTACCAACCAACCCTGCAGGAG TCAAGTACGATGTGAAGGCTTTGTGTGTGCACAGACAGGGAGGTGTGTGAATCGCAGACTCCTTTGCAAT GGGGACAATGACTGTGGAGACCAATCAGATGAAGCCAACTGTAGGAGGATTTATAAAAACTGTCAACGGG AGATGGAGCAGTACTGGGCAATTGACAGGCTGGCCAGTGGGATAAATCTGTTCACAAACACTTTTGAGGG CCCAGTTCTCGATCACCGGTATTATGCTGGTGGATGCTCCCCCCACTACATCCTGGACACCAACTTTAGG AAGCCATACAACGTAGAAAGCTACACCCCACAGACCAAATGTGAATATGAATTCACACTTACAGAATATG AATCATATTCAGATTTTGAACGTCTAGTGATAGAAAAGAAAACCCACATGTTTAATTTCACGTCTGGTTT TAAAGTGGATGGCGTTATGGATCTTGGAATTAAAGTAGAAAGCAACGAAGGCAAAAATTATGTGACCAGA ACCAAACGCTTCGCCCACACTCAAAGCAAGTTCCTCCACGCGCGCTCTGTCCTTGAAGTGGCACATTACA AGCTGAAGTCTAGAAGCCTCATGCTCCATTATGAATTCCTTCAGAGGGTCAAGAGCCTGCCCCTGGAGTA TAGCTATGGGGAATACCGAGATCTTCTTCGTGACTTCGGGACCCATTTCATCACTGAAGCCGTACTTGGG GGCATCTACGAGTACACGCTTATCATGAACAAAGACGCCATGGAGCAAGGAGACTACACTCTTAGCCATG TTACTGCCTGTGCTGGAGGAAGTTTCGGTATTGGTGGTATGGTCTATAAAGTCTATGTCAAAGTGGGCGT TTCCGCAAAGAAATGCAGTGACATTATGAAAGAAATCAACGAAAGAAATAAGAGGAGCACCATGGTAGAA GACTTGGTAGTTCTTGTGAGAGGAGGGACAAGTGAGGATATCACTGCCTTGGCATACAAGGAGCTACCAA CCCCTGAACTGATGGAGGCATGGGGCGATGCTGTGAAGTACAACCCAGCAATCATTAAGATCAAGGCTGA GCCCCTGTATGAGCTTGTGACAGCCACAGACTTTGCATACTCCAGCACAGTGAAGCAGAACTTAAAGAAG GCACTAGAAGAATTCCAGAGTGAGGTCAGCTCCTGCCGCTGTGCTCCTTGCAGAGGGAATGGAGTCCCTG TCCTGAAAGGATCCCGCTGTGAGTGCATCTGCCCCGGTGGCTTCCAAGGCACAGCCTGTGAGGTTACCTA TCGGAAAGACATCCCCATAGATGGGAAGTGGAGTTGCTGGTCTGACTGGTCTGCATGCTCTGGAGGACAC AAAACAAGACACAGGCAGTGCAACAATCCAGCCCCTCACAAAGGAGGCAGCCCCTGTTCGGGTCCTGCTT CCGAAACACTCAACTGT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR209183 protein sequence
Red=Cloning site Green=Tags(s) MKIGAQVWRALAKSCLLCATLGCLHFPGSRGGKPDFFETKAVNGSLVKSRPVRSVAEAPAPIDCELSTWS SWTACDPCQKKRYRHTYLLRPSQFYGELCDLSDKEVEDCVTNQPCRSQVRCEGFVCAQTGRCVNRRLLCN GDNDCGDQSDEANCRRIYKNCQREMEQYWAIDRLASGINLFTNTFEGPVLDHRYYAGGCSPHYILDTNFR KPYNVESYTPQTKCEYEFTLTEYESYSDFERLVIEKKTHMFNFTSGFKVDGVMDLGIKVESNEGKNYVTR TKRFAHTQSKFLHARSVLEVAHYKLKSRSLMLHYEFLQRVKSLPLEYSYGEYRDLLRDFGTHFITEAVLG GIYEYTLIMNKDAMEQGDYTLSHVTACAGGSFGIGGMVYKVYVKVGVSAKKCSDIMKEINERNKRSTMVE DLVVLVRGGTSEDITALAYKELPTPELMEAWGDAVKYNPAIIKIKAEPLYELVTATDFAYSSTVKQNLKK ALEEFQSEVSSCRCAPCRGNGVPVLKGSRCECICPGGFQGTACEVTYRKDIPIDGKWSCWSDWSACSGGH KTRHRQCNNPAPHKGGSPCSGPASETLNC TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_133882 |
| ORF Size | 1770 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_133882.2, NP_598643.1 |
| RefSeq Size | 2239 bp |
| RefSeq ORF | 1770 bp |
| Locus ID | 110382 |
| UniProt ID | Q8BH35 |
| MW | 66.2 kDa |
| Gene Summary | This gene encodes the beta subunit of complement component C8 that participates in the assembly of the complement membrane attack complex. The encoded preproprotein undergoes proteolytic processing to generate the beta subunit, which associates with the alpha and gamma subunits to form a trimeric complement component, C8. Alternative splicing results in multiple transcript variants encoding different isoforms that may undergo similar proteolytic processing. This gene is located adjacent to the gene encoding the alpha subunit. [provided by RefSeq, Oct 2015] |
Documents
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| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC219449 | C8b (untagged) - Mouse complement component 8, beta polypeptide (C8b), (10ug) |
CNY 7,510.00 |
|
| MG209183 | C8b (tGFP-tagged) - Mouse complement component 8, beta subunit (C8b) |
CNY 5,040.00 |
|
| MR209183L3 | Lenti ORF clone of C8b (Myc-DDK-tagged) - Mouse complement component 8, beta polypeptide (C8b) |
CNY 6,460.00 |
|
| MR209183L4 | Lenti ORF clone of C8b (mGFP-tagged) - Mouse complement component 8, beta polypeptide (C8b) |
CNY 6,460.00 |
