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Ptpn6 (NM_013545) Mouse Tagged ORF Clone
CAT＃: MR209258
- TrueORF®
Ptpn6 (Myc-DDK-tagged) - Mouse protein tyrosine phosphatase, non-receptor type 6 (Ptpn6), transcript variant 1
"NM_013545" in other vectors (3)
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CNY 6,656.00
| Cited in 1 publication. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 70Z-SHP; hcp; Hcph; me; Ptp1C; PTPTY-42; SH-PTP1; SHP-1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR209258 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTGAGGTGGTTTCACCGGGACCTCAGCGGGCCTGATGCAGAGACCCTGCTAAAGGGCCGGGGAGTCC CTGGGAGCTTCCTGGCTCGGCCCAGCCGCAAGAACCAGGGTGACTTCTCCCTCTCAGTCAGGGTGGATGA TCAGGTGACTCATATTCGGATCCAGAACTCAGGGGACTTCTATGACCTGTACGGAGGGGAGAAGTTTGCG ACGCTGACAGAGCTGGTCGAGTATTACACGCAGCAGCAGGGCATCCTGCAGGACCGAGATGGCACCATCA TCCACCTTAAGTACCCACTGAACTGCTCGGACCCCACCAGTGAGAGGTGGTACCACGGCCACATATCTGG AGGGCAGGCGGAGTCACTGCTGCAGGCCAAGGGCGAGCCCTGGACATTTCTTGTGCGTGAGAGTCTCAGC CAACCTGGTGATTTTGTGCTCTCTGTGCTCAATGACCAGCCCAAGGCTGGCCCAGGTTCCCCGCTCAGGG TCACTCATATCAAGGTTATGTGTGAGGGTGGACGCTATACTGTGGGTGGCTCAGAGACGTTTGACAGCCT CACAGACCTGGTGGAGCACTTCAAGAAGACAGGGATTGAGGAGGCCTCGGGTGCCTTTGTCTACCTGCGG CAGCCTTACTACGCCACTCGGGTAAACGCAGCTGACATTGAGAATCGGGTCTTGGAACTGAACAAGAAGC AGGAGTCGGAGGACACAGCCAAGGCTGGCTTCTGGGAGGAGTTTGAGAGTCTACAAAAGCAGGAGGTAAA GAATCTACACCAACGTCTGGAAGGGCAGCGGCCGGAGAACAAGAGCAAGAACCGCTACAAGAACATTCTT CCCTTTGACCACAGCCGAGTGATCCTGCAGGGACGTGACAGTAACATCCCAGGCTCTGACTACATCAATG CCAACTACGTGAAGAACCAGCTGCTAGGTCCAGATGAGAACTCTAAGACCTACATCGCCAGCCAGGGCTG TCTGGATGCCACAGTCAATGACTTCTGGCAGATGGCTTGGCAGGAGAACACTCGTGTCATCGTCATGACT ACCAGAGAGGTGGAGAAAGGCCGGAACAAATGTGTCCCATACTGGCCCGAGGTGGGCACTCAGCGTGTCT ATGGTCTCTACTCTGTGACCAACAGTAGGGAGCATGACACAGCAGAATACAAACTGCGAACATTACAGAT CTCCCCACTAGACAATGGGGACCTGGTTCGGGAGATATGGCACTACCAGTACCTGAGCTGGCCTGACCAT GGGGTTCCCAGTGAGCCTGGGGGTGTCCTCAGCTTTCTGGATCAGATCAACCAGCGACAGGAAAGTTTGC CTCATGCAGGGCCCATCATTGTGCATTGCAGCGCTGGCATCGGCCGCACGGGCACCATCATCGTCATTGA TATGCTTATGGAAAGCATCTCCACCAAGGGGCTAGACTGTGACATTGATATCCAGAAGACCATCCAGATG GTACGAGCACAGCGCTCCGGCATGGTGCAGACCGAGGCCCAGTACAAGTTTATTTACGTGGCCATTGCCC AGTTCATCGAAACGACCAAGAAGAAACTGGAGATCATACAATCCCAGAAGGGCCAGGAGTCGGAGTATGG GAATATCACGTACCCTCCCGCTGTGAGGAGTGCCCACGCCAAAGCCTCGCGTACTTCCTCCAAGCACAAG GAGGAGGTGTACGAAAACGTGCATAGCAAGAGCAAGAAGGAAGAGAAAGTAAAGAAGCAGCGGTCGGCAG ACAAGGATAAGAACAAAGGTTCTCTCAAGAGGAAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR209258 protein sequence
Red=Cloning site Green=Tags(s) MVRWFHRDLSGPDAETLLKGRGVPGSFLARPSRKNQGDFSLSVRVDDQVTHIRIQNSGDFYDLYGGEKFA TLTELVEYYTQQQGILQDRDGTIIHLKYPLNCSDPTSERWYHGHISGGQAESLLQAKGEPWTFLVRESLS QPGDFVLSVLNDQPKAGPGSPLRVTHIKVMCEGGRYTVGGSETFDSLTDLVEHFKKTGIEEASGAFVYLR QPYYATRVNAADIENRVLELNKKQESEDTAKAGFWEEFESLQKQEVKNLHQRLEGQRPENKSKNRYKNIL PFDHSRVILQGRDSNIPGSDYINANYVKNQLLGPDENSKTYIASQGCLDATVNDFWQMAWQENTRVIVMT TREVEKGRNKCVPYWPEVGTQRVYGLYSVTNSREHDTAEYKLRTLQISPLDNGDLVREIWHYQYLSWPDH GVPSEPGGVLSFLDQINQRQESLPHAGPIIVHCSAGIGRTGTIIVIDMLMESISTKGLDCDIDIQKTIQM VRAQRSGMVQTEAQYKFIYVAIAQFIETTKKKLEIIQSQKGQESEYGNITYPPAVRSAHAKASRTSSKHK EEVYENVHSKSKKEEKVKKQRSADKDKNKGSLKRK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_013545 |
| ORF Size | 1788 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_013545.1, NM_013545.2, NM_013545.3, NP_038573.2 |
| RefSeq Size | 2219 bp |
| RefSeq ORF | 1788 bp |
| Locus ID | 15170 |
| UniProt ID | P29351 |
| MW | 67.5 kDa |
| Gene Summary | Modulates signaling by tyrosine phosphorylated cell surface receptors such as KIT and the EGF receptor/EGFR. The SH2 regions may interact with other cellular components to modulate its own phosphatase activity against interacting substrates. Together with MTUS1, induces UBE2V2 expression upon angiotensin II stimulation. Plays a key role in hematopoiesis.[UniProtKB/Swiss-Prot Function] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Regulation of Microtubule Nucleation in Mouse Bone Marrow-Derived Mast Cells by Protein Tyrosine Phosphatase SHP-1
,Klebanovych, A;Sládková, V;Sulimenko, T;Vosecká, V;Čapek, M;Dráberová, E;Dráber, P;Sulimenko, V;,
Cells
,PubMed ID 30979083
[PTPN6]
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Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC219479 | Ptpn6 (untagged) - Mouse protein tyrosine phosphatase, non-receptor type 6 (Ptpn6), transcript variant 1, (10ug) |
CNY 7,700.00 |
|
| MR209258L3 | Lenti ORF clone of Ptpn6 (Myc-DDK-tagged) - Mouse protein tyrosine phosphatase, non-receptor type 6 (Ptpn6), transcript variant 1 |
CNY 6,460.00 |
|
| MR209258L4 | Lenti ORF clone of Ptpn6 (mGFP-tagged) - Mouse protein tyrosine phosphatase, non-receptor type 6 (Ptpn6), transcript variant 1 |
CNY 6,460.00 |
