Ankrd25 (BC010245) Mouse Tagged ORF Clone

CAT#: MR209360

  • TrueORF®

Ankrd25 (Myc-DDK-tagged) - Mouse ankyrin repeat domain 25 (cDNA clone MGC:12143 IMAGE:3710765)

ORF Plasmid: DDK tGFP



  "BC010245" in other vectors (4)

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Mouse Protein / Lysate Request

CNY 6,080.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Synonyms MGC12143, MGC7734
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR209360 representing BC010245
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCCAGGTCCTGCATGTGCCCGCCCCATTCCCAGGAACCCCTGGTCAGGCCTCCCCAGCGGCCTTCC
CCAGCAAGGAGCCAGACCCACCGTACTCAGTGGAAACGCCCTATGGCTACCGCCTGGACCTGGATTTTCT
TAAGTACGTGGATGACATTGAGAAGGGCCACACACTGCGCAGGGTGGCGGTACAGCGCCGGCCGCGCCTG
GGTTCCCTGCCACGGGGCCCGGGCTCCTGGTGGACGTCCACAGAGTCTCTGTGCTCTGATGCCAGTGGGG
ACAGCCGGCACTCGGCCTACTCCTACTGTGGCCGCGGCTTCTACCCACAGTACGGAGCCTTGGAGACCCG
AATCGGCTCCAACCCTCGTGTGGAGCGCACGCTGCTGGATGCGCGGCGCCGGCTGGAGGACCAGGTGGCT
GCCCCATCGTCCGGTGGCCTGGGCTCCCTCACACCCAGCGCCGCGGGCTCCACCAGCTCCCTGGCAGGCG
TGGGCCTGCTGCCCCCCACGCCTCGCAGCTCCGGCCTGTCCACCCCGGTGGCACCCAGCGCTGGTCACCT
AGCCCACGTGCGCGAGCAGATGGCTGGAGCCCTGCGCAAGCTGCGCCAGCTGGAGGAGCAGGTCAAGCTG
ATCCCCGTTCTCCAAGTCAAGCTGTCAGTGCTCCAGGAGGAAAAGAGACAGCTCACCGTGCAGCTCAAGA
GCCAGAAGTTTCTAGGTCACCCCTCAGGGACACGGAGCCGGAGTGAGCTCTGCCTGGACCTTCCCGAGGC
CCCTGACGACCCTGCTGCACTAGAGACCCGCTCGGTGGGCACCTGGGTTAGAGAACGGGACTTGGGCATT
CCAGATGGTGAAGCTGCCCTGGTAGCTAAAGTGGCCGTGTTGGAGACCCAGCTCAAAAAAGCGCTACAGG
AGCTGCGGGCGGCTCAGACCCAGCAGGTCGACCTCCAGCCCCAGGCCTGGCCACCACCAGACACCCAGGT
CCGCGTGGACACCGTGCGAGTAGTGGAGGGTCCCCGGGAAGTGGAGGTGGCAGCCAGCACGGCAGCCGGG
GCCCTTGCACAGCGAGCTCAGAGTCTGGAACCCTACGGAACAGGGCTGAAGGCCCTGACCACATCTGGGG
GACCAGAGAACACCCTCGTGTTCCGCAGTCATGAAGTGGTGGAGACCATGTGCCCACTGCCCACAGCTAC
CACTGGCAATGTGCACACAGCCAAGAAGATCAGCATTACAGAACGGAGTTGCACCGGTGCACCCAGGATG
ACAGAGCCTTCCTCCGTTAATCCACGCCCTGCTGCAGCCTCTGTGGTACAGCCTGAGAACCCTGTCCCGG
CTGCCCAGGACACCACTGACAAGAAGCCCACCAGGCCAGCAGCAGCATCGCAGGACTCACAAGCGGCTGA
CGGAGCAGGCAGAGCCTCTTTGGCCACGAAGAGGAAAGAGGACCCCGCAGACCCTGAAGTCAACCAGAGG
AACCTCCAGTTTGTGGGAGTCAATGGCGGGTATGAGTCCCCCTCGGAGGACTCCAGCACAGCAGAAAACT
CCGAACACGAGAGCACAGAGAACGAGGGCCCGGAGCCACCAGCAAGGGTTCTGAGCCCAGCTGAATGTCC
TCAGCTCAGGCCTCCAGGGGCTGCAGTGGCCACGACCAGTCTGGAGGGGCCCCAGCTCTCCCAGGAATCC
CAGCGTGTGCCTGCACCTGAGGTGGCGTCAGGACCCGATCCTGAGGAGGAAATTCGAAAGTGGCCTATAC
CACAGTGCTTCAGGAGTGGCTGCGCCTGGCCTGCCGCAGTGACGCACACCCTGAGCTTGTGCGGCGACAT
C


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR209360 representing BC010245
Red=Cloning site Green=Tags(s)

MAQVLHVPAPFPGTPGQASPAAFPSKEPDPPYSVETPYGYRLDLDFLKYVDDIEKGHTLRRVAVQRRPRL
GSLPRGPGSWWTSTESLCSDASGDSRHSAYSYCGRGFYPQYGALETRIGSNPRVERTLLDARRRLEDQVA
APSSGGLGSLTPSAAGSTSSLAGVGLLPPTPRSSGLSTPVAPSAGHLAHVREQMAGALRKLRQLEEQVKL
IPVLQVKLSVLQEEKRQLTVQLKSQKFLGHPSGTRSRSELCLDLPEAPDDPAALETRSVGTWVRERDLGI
PDGEAALVAKVAVLETQLKKALQELRAAQTQQVDLQPQAWPPPDTQVRVDTVRVVEGPREVEVAASTAAG
ALAQRAQSLEPYGTGLKALTTSGGPENTLVFRSHEVVETMCPLPTATTGNVHTAKKISITERSCTGAPRM
TEPSSVNPRPAAASVVQPENPVPAAQDTTDKKPTRPAAASQDSQAADGAGRASLATKRKEDPADPEVNQR
NLQFVGVNGGYESPSEDSSTAENSEHESTENEGPEPPARVLSPAECPQLRPPGAAVATTSLEGPQLSQES
QRVPAPEVASGPDPEEEIRKWPIPQCFRSGCAWPAAVTHTLSLCGDI

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC010245
ORF Size 1821 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC010245.1
RefSeq Size 3427 bp
RefSeq ORF 1823 bp
Locus ID 235041
MW 125.6 kDa
Gene Summary Involved in transcription regulation by sequestering in the cytoplasm nuclear receptor coactivators such as NCOA1, NCOA2 and NCOA3 (By similarity). Involved in regulation of caspase-independent apoptosis by sequestering the proapoptotic factor AIFM1 in mitochondria (By similarity). Pro-apoptotic stimuli can induce its proteasomal degradation allowing the translocation of AIFM1 to the nucleus to induce apoptosis (By similarity). Involved in the negative control of vitamin D receptor signaling pathway (By similarity). Involved in actin stress fibers formation through its interaction with ARHGDIA and the regulation of the Rho signaling pathway (PubMed:25961457). May thereby play a role in cell adhesion and migration, regulating for instance podocytes migration during development of the kidney (PubMed:25961457). Through the Rho signaling pathway may also regulate cell proliferation (PubMed:16024821).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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