United States
Germany
Japan
United Kingdom
China
Prr14 (NM_145589) Mouse Tagged ORF Clone
CAT＃: MR209414
- TrueORF®
Prr14 (Myc-DDK-tagged) - Mouse proline rich 14 (Prr14)
ORF Plasmid: tGFP
"NM_145589" in other vectors (4)
Need custom modification / cloning service?
Get a free quote
CNY 6,080.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | BC006909 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR209414 representing NM_145589
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACTTGCCTGGGAACTCCAGCCCTTTTACACAGCCAAGCCTGTGCCGTCAGCCTCTATCTCGAGCAT CATGGGAAGCCAGGAGCCCGAAAAGACCGAGGCTGCAGCCTTTGGGGACCCCTTCATCCCTAGAAAAGGC CTCTCGGAGGGTCCTTGCCGTGGTGTTGGAAGATGTCATGACTACAAACAGGGTCCCCCTGACACACAAA GAGGACACTCCCAGCCCACTGACTCACAACCATCATCAGGATCCTGTCTGCACACAGTCACCTGCTTTAC CATCCCAACAGGTCAAGTGGTCCATGCAAGCCAGGCCTCCTGACCCACTGCATTTGTGCAGAGAACCCTT GACTCGAGCCCGCCAGTCCTCGCCTGCCTTGAGAATGAGATCAAGGGCAGCATCTGGCCCAGAGGAGAGC CCCTCAAAAAAGACAGACCAGGTCCCACAGCCCACCCTAGTGGTGGTACTGGAAGACATTGCCAGTGGCA GACAACCAGCAGAGGGTTTTGATGAAGATCAGCCCAACCTCATTGTCCCAGCACAAAGCACCTTCAGAAG CCTGAAGGGCCCGGGGAAGCACTGCCACAGAAGGGGTCTGGACTTGGAAGCACGCCCAACTCTTACTTTG TCCCTTCACCCGAGAGCTGAGCCTGTGACAAAAGCTGGTCAGCCAATGCCTACACCCAGTGACCTAGAAC CCCCATTCCAACTGTCTACTCTACCTGCAGATCCTCCAGAGAGTCCAGTACCAGATCCTGCTCTGGAGAC CCCGGTGATTCCAACATCGTCAAGCCTTTTACGACCCCGTCTCAGTCCCTGGGGCTTGGCCCCTCTCTTC CGTTCCGTCCGATCAAAGCTTGAAAGCTTTGCTGATATCTTCTTCACACCTAACAAAACACCACAGCCTC CACCCCCTTCACCTCCCATGAAATTAGAATTGAAGATTGCCATCTCGGAGGCTGAGCAGTCCAGGGCTAC TGAGAAAATTACATCTGTCAGCCCCAGGCCCCCTATCCGCCAGTGGCGGACTCAGTGCAATTCCCTAGCA CCTGTCTCTAAGTCATCTCTGGGACGAAGCTACTCCTGCCCTGATCTAGGGCCCCCTGACCCAGGTAGCT GGCCTCCTGTCCCATCCCAGCCAAGCCAGTCCAGGCCCCGGAGACATACTGTGGGTTGTGGGGAGATGGC CCGGACCCCACCACCTCCTCGACCCTGTCTCCGGAAAGAGGTCTTCCCTCTTGGAGGAGTGGGAGTCTCT CCTTCTCTCACTACATCTTGCTCAGCCAATGCCCCTGCTTCCTTCTTCTGCGAGCCGGCAGAACCCAGGT TGGGTTCAACGAAGGGGAAGGAGTTAAGAGCCTCAAAAGACAAGGTGTTTTCTGATCCAGAGACCAAGAC CATGGGGAAGGTTTCTAGATTCAGAATACGGAGGACACCTGTCCGTCTCCAACCAAACCTTACACCAATG GGGCTGCCTCGACCAATCAGGTTGAATAAGAAGGAGTTCACTTTGGAAGAAATTTATACAAATAAGAACT ACCAATCACCCACAACCAGAAGGACCTTTGAGACCATCTTTGAGGAACCACGGGAGCGCAATGGAACTCT GATTTTTACCAGCTCTAGGAAGCTCCGAAGGGCAGTAGAATTTCGGGACAGTAGCCTTCCTCGATCCCGG CGACCATCACGAGGGGTCCGCACCGCAGCAAGCAGAACCCTTACTCCCAACCTGGCACCCAGCCAGGATG TGGGGTCTCTACTGCAAGAAAGGCTGAGGGAGCTAGATGCCTTGCTCCTGGAGGAGGAGACAGATAAGGA ACACCCTTGTCACCTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR209414 representing NM_145589
Red=Cloning site Green=Tags(s) MDLPGNSSPFTQPSLCRQPLSRASWEARSPKRPRLQPLGTPSSLEKASRRVLAVVLEDVMTTNRVPLTHK EDTPSPLTHNHHQDPVCTQSPALPSQQVKWSMQARPPDPLHLCREPLTRARQSSPALRMRSRAASGPEES PSKKTDQVPQPTLVVVLEDIASGRQPAEGFDEDQPNLIVPAQSTFRSLKGPGKHCHRRGLDLEARPTLTL SLHPRAEPVTKAGQPMPTPSDLEPPFQLSTLPADPPESPVPDPALETPVIPTSSSLLRPRLSPWGLAPLF RSVRSKLESFADIFFTPNKTPQPPPPSPPMKLELKIAISEAEQSRATEKITSVSPRPPIRQWRTQCNSLA PVSKSSLGRSYSCPDLGPPDPGSWPPVPSQPSQSRPRRHTVGCGEMARTPPPPRPCLRKEVFPLGGVGVS PSLTTSCSANAPASFFCEPAEPRLGSTKGKELRASKDKVFSDPETKTMGKVSRFRIRRTPVRLQPNLTPM GLPRPIRLNKKEFTLEEIYTNKNYQSPTTRRTFETIFEEPRERNGTLIFTSSRKLRRAVEFRDSSLPRSR RPSRGVRTAASRTLTPNLAPSQDVGSLLQERLRELDALLLEEETDKEHPCHL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_145589 |
| ORF Size | 1836 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_145589.2, NP_663564.2 |
| RefSeq Size | 2325 bp |
| RefSeq ORF | 1839 bp |
| Locus ID | 233895 |
| UniProt ID | Q7TPN9 |
| MW | 68.3 kDa |
| Gene Summary | Functions in tethering peripheral heterochromatin to the nuclear lamina during interphase, possibly through the interaction with heterochromatin protein CBX5/HP1 alpha (By similarity). Might play a role in reattaching heterochromatin to the nuclear lamina at mitotic exit (By similarity). Promotes myoblast differentiation during skeletal myogenesis, possibly by stimulating transcription factor MyoD activity via binding to CBX5/HP1 alpha (PubMed:25906157) (By similarity). Involved in the positive regulation of the PI3K-Akt-mTOR signaling pathway and in promoting cell proliferation, possibly via binding to GRB2 (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC204769 | Prr14 (untagged) - Mouse proline rich 14 (Prr14), (10ug) |
CNY 4,568.00 |
|
| MG209414 | Prr14 (tGFP-tagged) - Mouse proline rich 14 (Prr14) |
CNY 8,888.00 |
|
| MR209414L3 | Lenti ORF clone of Prr14 (Myc-DDK-tagged) - Mouse proline rich 14 (Prr14) |
CNY 7,980.00 |
|
| MR209414L4 | Lenti ORF clone of Prr14 (mGFP-tagged) - Mouse proline rich 14 (Prr14) |
CNY 7,980.00 |
