Atp6v1a (NM_007508) Mouse Tagged ORF Clone
CAT#: MR209449
- TrueORF®
Atp6v1a (Myc-DDK-tagged) - Mouse ATPase, H+ transporting, lysosomal V1 subunit A (Atp6v1a)
ORF Plasmid: tGFP
"NM_007508" in other vectors (4)
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CNY 4,592.00
Cited in 1 publication. |
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | AI647066; Atp6a1; Atp6a2; Atp6v1a1; VA68; VPP2 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR209449 representing NM_007508
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATTTCTCCAAGCTACCCAAAATCCGCGATGAGGATAAAGAAAGTACATTTGGTTATGTGCATGGAG TCTCAGGGCCTGTGGTTACAGCCTGTGACATGGCGGGCGCTGCCATGTACGAGCTGGTGAGAGTGGGGCA CAGCGAGCTGGTTGGAGAAATTATTCGATTGGAAGGTGACATGGCCACCATTCAGGTGTATGAAGAGACT TCTGGTGTCTCTGTTGGAGACCCCGTACTCCGCACTGGTAAACCTCTCTCGGTCGAGCTGGGTCCCGGGA TTATGGGAGCCATTTTTGATGGTATACAGAGACCTCTGTCGGATATCAGCAGTCAGACCCAAAGTATCTA CATCCCCAGAGGAGTCAATGTGTCTGCTCTCAGCAGAGATATCAAATGGGAGTTTATACCCAGCAAAAAC CTACGGGTTGGTAGTCATATCACTGGTGGAGACATTTATGGGATTGTCAATGAGAACTCCCTCATCAAAC ACAAAATCATGTTGCCCCCACGTAACAGAGGAAGCGTGACTTACATCGCGCCGCCTGGGAATTATGATGC GTCCGATGTCGTCCTGGAGCTTGAGTTTGAAGGTGTGAAGGAGAAGTTCAGCATGGTCCAAGTGTGGCCT GTGCGGCAGGTGCGGCCTGTCACTGAGAAGCTGCCCGCCAATCACCCCTTGCTTACTGGCCAGAGAGTCC TCGATGCCCTTTTCCCGTGTGTTCAGGGAGGAACTACTGCTATCCCAGGCGCCTTTGGCTGTGGAAAGAC TGTGATTTCCCAGTCTCTATCCAAGTACTCCAACAGTGACGTCATCATCTATGTCGGCTGCGGTGAGAGA GGCAACGAGATGTCAGAAGTTCTCCGCGACTTCCCTGAGCTCACCATGGAGGTTGATGGGAAAGTAGAGT CCATCATGAAGAGGACAGCGCTGGTAGCCAACACCTCCAACATGCCTGTGGCTGCAAGAGAGGCCTCCAT CTACACTGGAATTACACTATCAGAATATTTCCGTGACATGGGCTACCACGTCAGTATGATGGCGGACTCT ACCTCTAGATGGGCTGAGGCCCTCAGAGAAATCTCTGGTCGCTTAGCTGAGATGCCTGCAGATAGTGGAT ACCCTGCATACCTTGGTGCCCGGCTGGCTTCTTTCTATGAGCGAGCAGGCAGAGTGAAATGTCTTGGAAA CCCTGAGAGAGAAGGGAGTGTCAGCATTGTAGGAGCAGTTTCTCCACCTGGTGGTGATTTTTCTGATCCA GTCACTTCTGCAACTCTGGGTATTGTTCAGGTGTTCTGGGGCTTGGATAAGAAGTTAGCTCAGCGCAAGC ACTTCCCCTCTGTCAACTGGCTCATCAGCTACAGCAAGTACATGCGTGCCCTGGACGAGTACTATGACAA GCACTTCACGGAGTTTGTTCCTCTGAGGACCAAAGCTAAGGAGATCCTGCAGGAAGAAGAGGATCTGGCA GAAATCGTGCAGCTCGTGGGAAAGGCCTCTTTAGCAGAGACGGATAAAATCACTCTGGAGGTAGCAAAAC TTATTAAAGATGACTTCCTACAGCAAAATGGGTACACTCCTTATGACAGGTTCTGTCCATTCTACAAGAC AGTGGGGATGCTGTCCAACATGATTTCATTCTATGACATGGCCCGCCGAGCTGTGGAGACCACTGCCCAG AGTGACAATAAGATCACATGGTCCATTATCCGGGAGCACATGGGGGAGATTCTCTATAAACTCTCCTCCA TGAAATTCAAGGATCCAGTGAAAGATGGCGAGGCAAAGATCAAGGCCGACTATGCACAGCTTCTTGAAGA TATGCAGAATGCATTCCGTAGCCTTGAAGAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR209449 representing NM_007508
Red=Cloning site Green=Tags(s) MDFSKLPKIRDEDKESTFGYVHGVSGPVVTACDMAGAAMYELVRVGHSELVGEIIRLEGDMATIQVYEET SGVSVGDPVLRTGKPLSVELGPGIMGAIFDGIQRPLSDISSQTQSIYIPRGVNVSALSRDIKWEFIPSKN LRVGSHITGGDIYGIVNENSLIKHKIMLPPRNRGSVTYIAPPGNYDASDVVLELEFEGVKEKFSMVQVWP VRQVRPVTEKLPANHPLLTGQRVLDALFPCVQGGTTAIPGAFGCGKTVISQSLSKYSNSDVIIYVGCGER GNEMSEVLRDFPELTMEVDGKVESIMKRTALVANTSNMPVAAREASIYTGITLSEYFRDMGYHVSMMADS TSRWAEALREISGRLAEMPADSGYPAYLGARLASFYERAGRVKCLGNPEREGSVSIVGAVSPPGGDFSDP VTSATLGIVQVFWGLDKKLAQRKHFPSVNWLISYSKYMRALDEYYDKHFTEFVPLRTKAKEILQEEEDLA EIVQLVGKASLAETDKITLEVAKLIKDDFLQQNGYTPYDRFCPFYKTVGMLSNMISFYDMARRAVETTAQ SDNKITWSIIREHMGEILYKLSSMKFKDPVKDGEAKIKADYAQLLEDMQNAFRSLED TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_007508 |
ORF Size | 1851 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_007508.2 |
RefSeq Size | 3947 bp |
RefSeq ORF | 1854 bp |
Locus ID | 11964 |
UniProt ID | P50516 |
MW | 68.8 kDa |
Gene Summary | Catalytic subunit of the peripheral V1 complex of vacuolar ATPase. V-ATPase vacuolar ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells. In aerobic conditions, involved in intracellular iron homeostasis, thus triggering the activity of Fe(2+) prolyl hydroxylase (PHD) enzymes, and leading to HIF1A hydroxylation and subsequent proteasomal degradation. May play a role in neurite development and synaptic connectivity.[UniProtKB/Swiss-Prot Function] |
Citations (1)
The use of this cDNA Clones has been cited in the following citations: |
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Specific autoantigens identified by sera obtained from mice that are immunized with testicular germ cells alone
,Terayama, H;Hirai, S;Naito, M;Qu, N;Katagiri, C;Nagahori, K;Hayashi, S;Sasaki, H;Moriya, S;Hiramoto, M;Miyazawa, K;Hatayama, N;Li, ZL;Sakabe, K;Matsushita, M;Itoh, M;,
Sci Rep
,PubMed ID 27752123
[ATP6V1A]
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Documents
Product Manuals |
FAQs |
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Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC202829 | Atp6v1a (untagged) - Mouse ATPase, H+ transporting, lysosomal V1 subunit A (Atp6v1a), (10ug) |
CNY 4,600.00 |
|
MG209449 | Atp6v1a (tGFP-tagged) - Mouse ATPase, H+ transporting, lysosomal V1 subunit A (Atp6v1a) |
CNY 5,040.00 |
|
MR209449L3 | Lenti ORF clone of Atp6v1a (Myc-DDK-tagged) - Mouse ATPase, H+ transporting, lysosomal V1 subunit A (Atp6v1a) |
CNY 6,460.00 |
|
MR209449L4 | Lenti ORF clone of Atp6v1a (mGFP-tagged) - Mouse ATPase, H+ transporting, lysosomal V1 subunit A (Atp6v1a) |
CNY 6,460.00 |
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