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Golga2 (BC011407) Mouse Tagged ORF Clone
CAT＃: MR209451
- TrueORF®
Golga2 (Myc-DDK-tagged) - Mouse golgi autoantigen, golgin subfamily a, 2 (cDNA clone MGC:11816 IMAGE:3596079)
ORF Plasmid: tGFP
"BC011407" in other vectors (4)
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CNY 4,592.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | GM130, golgin-95, GM-130, GM 130, mKIAA4150 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR209451 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAATCACTGAAGCAGCTCCAGGTGGAGAGAGACCAGTATGCGGAGAACCTAAAAGGAGAGAGTGCCA TGTGGCAGCAGAGGGTACAGCAAATGGCGGAGCAGGTGCATACACTGAAGGAAGAGAAGGAGCACAGAGA ACGGCAGGTACAAGAGCTGGAGACCAGCTTGGCCGCACTCCGGAGTCAGATGGAGGAGCCACCGCCTCCA GAGCCTCCAGCTGGGCCCTCCGAGGCGGAAGAGCAGCTGCAGGGAGAGGTCGAGCAGCTGCACAAGGAAC TGGAGAGGCTGACGGGACAGCTACGGGCTCAGGTGCAGGACAATGAGAGCCTGAGCCACCTCAACCGGGA GCAGGAGGGGCGTCTGCTGGAGCTGGAGCGGGAGGCCCAGCGCTGGAGCGAGCAGGCCGAGGAGCGCAAG CAGATCCTGGAGAGCATGCAGAGTGACCGCACCACCATCAGCAGAGCACTGTCACAGAACCGAGAGCTGA AGGAGCAGCTGGCCGAGCTGCAGAACGGCTTCGTCAGGCTGACCAATGAGAACATGGAGATCACCAGTGC GCTGCAGTCAGAGCAGCATGTCAAGAAAGAGCTAGCCCGGAAGCTGGGAGAGCTGCAGGAGAGACTGGGA GAGCTGAAGGAGACGGTGGAGCTCAAGAGCCAAGAAGCACAGGGTCTACAGGAGCAGCGAGACCAGTGCC TGTCCCACCTGCAGCAGTACGCTGCTGCTTACCAACAGCACTTGGCGGCCTATGAGCAGCTGACCTCTGA GAAGGAGGCAATACACAAGCAGCTTCTGCTGCAGACTCAGCTCATGGACCAGCTGCAGCATGAGGAGGTA CAGGGCAAGATGGCAGCCGAGCTGGCCCGCCAGGAGCTGCAGGAGGCCCAGGAGCGCCTAAAAGCTACCA GCCAGGAGAACCAACAGCTCCAAGCCCAGCTGAGCCTCTTGGTCCTCCCTGGGGAAGGAGATGTGGACCA GGAGGAAGAAGATGAGGAGGTTCCTCAGTCGAGTCTGGCCATCCCAGAAGACCTAGACAGTAGAGAGGCC ATGGTGGCATTCTTTAATGCCGCTATAGCCAGAGCAGAGGAAGAACAGGCCCGACTACGTGTGCAGCTGA AAGAGCAGAAGGCACGTTGCCGGAGTTTGTCTCACTTGGCAGCCCCAGTCCAAAGCAAGCTTGAAAAGGA GGCAGTGGTCCCCAGGAATGTGGATGACTCTGCATCTGAGGAGAGCAATCAGGCCCTACATGTAGCCATG GAGAAGCTGCAGAGCCGCTTCCTGGAGGTCATGCAGGAGAAAGTGGAGCTCAAGGAGAGGGTAGAGGAGC TTGAACATTGCTGCATTCAGCTTTCTGGAGAGACAGACACCATTGGAGAGTACATTGCCCTTTACCAAAA TCAGAGGGCGGTGCTGAAGGCTCGGCATTTGGAGAAGGAAGAATACATCAGCCGGCTGGCTCAGGACAAG GAGGAGATGAAGGTGAAGCTGCTGGAGCTCCAGGAGCTGGTGTTGAGGCTTGTGAACGAGCGCAATGAAT GGCAGGGCAAGTTCCTGGCCGTCTCTCAGAACCCTGGTGACGTGCTCACCCCAGTGCCCACAGGCTCCCA GGAATTTGGTGCTGCTGACCAGCAGGATGACCTCAGGGAGGTGAGCCTGGCTGACGATATAGAGCCTGCA CAAGGAGAGGCAGGGGTACCTGCTCCCCATGAGAACCCTACTGCACAGCAGATCATGCAGCTGCTGCGTG AGATCCAGAACCCCCGGGAGCGGCCAGGCCTGGGTAGCAACCCTTGCATCCCCTTTTTCTACCGTGCCGA TGAGAACGACGAGGTGAAGATCATGGTTGTA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR209451 protein sequence
Red=Cloning site Green=Tags(s) MESLKQLQVERDQYAENLKGESAMWQQRVQQMAEQVHTLKEEKEHRERQVQELETSLAALRSQMEEPPPP EPPAGPSEAEEQLQGEVEQLHKELERLTGQLRAQVQDNESLSHLNREQEGRLLELEREAQRWSEQAEERK QILESMQSDRTTISRALSQNRELKEQLAELQNGFVRLTNENMEITSALQSEQHVKKELARKLGELQERLG ELKETVELKSQEAQGLQEQRDQCLSHLQQYAAAYQQHLAAYEQLTSEKEAIHKQLLLQTQLMDQLQHEEV QGKMAAELARQELQEAQERLKATSQENQQLQAQLSLLVLPGEGDVDQEEEDEEVPQSSLAIPEDLDSREA MVAFFNAAIARAEEEQARLRVQLKEQKARCRSLSHLAAPVQSKLEKEAVVPRNVDDSASEESNQALHVAM EKLQSRFLEVMQEKVELKERVEELEHCCIQLSGETDTIGEYIALYQNQRAVLKARHLEKEEYISRLAQDK EEMKVKLLELQELVLRLVNERNEWQGKFLAVSQNPGDVLTPVPTGSQEFGAADQQDDLREVSLADDIEPA QGEAGVPAPHENPTAQQIMQLLREIQNPRERPGLGSNPCIPFFYRADENDEVKIMVV TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC011407 |
| ORF Size | 1851 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC011407, AAH11407 |
| RefSeq Size | 4174 bp |
| RefSeq ORF | 1853 bp |
| Locus ID | 99412 |
| MW | 70.7 kDa |
| Gene Summary | Peripheral membrane component of the cis-Golgi stack that acts as a membrane skeleton that maintains the structure of the Golgi apparatus, and as a vesicle thether that facilitates vesicle fusion to the Golgi membrane (PubMed:28028212). Required for normal protein transport from the endoplasmic reticulum to the Golgi apparatus and the cell membrane (PubMed:28028212). Together with p115/USO1 and STX5, involved in vesicle tethering and fusion at the cis-Golgi membrane to maintain the stacked and inter-connected structure of the Golgi apparatus. Plays a central role in mitotic Golgi disassembly: phosphorylation at Ser-37 by CDK1 at the onset of mitosis inhibits the interaction with p115/USO1, preventing tethering of COPI vesicles and thereby inhibiting transport through the Golgi apparatus during mitosis. Also plays a key role in spindle pole assembly and centrosome organization (By similarity). Promotes the mitotic spindle pole assembly by activating the spindle assembly factor TPX2 to nucleate microtubules around the Golgi and capture them to couple mitotic membranes to the spindle: upon phosphorylation at the onset of mitosis, GOLGA2 interacts with importin-alpha via the nuclear localization signal region, leading to recruit importin-alpha to the Golgi membranes and liberate the spindle assembly factor TPX2 from importin-alpha. TPX2 then activates AURKA kinase and stimulates local microtubule nucleation. Upon filament assembly, nascent microtubules are further captured by GOLGA2, thus linking Golgi membranes to the spindle (By similarity). Regulates the meiotic spindle pole assembly, probably via the same mechanism (PubMed:21552007). Also regulates the centrosome organization (By similarity). Also required for the Golgi ribbon formation and glycosylation of membrane and secretory proteins (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC200685 | Golga2 (untagged) - Mouse golgi autoantigen, golgin subfamily a, 2 (cDNA clone MGC:11816 IMAGE:3596079), (10ug) |
CNY 2,000.00 |
|
| MG209451 | Golga2 (tGFP-tagged) - Mouse golgi autoantigen, golgin subfamily a, 2 (cDNA clone MGC:11816 IMAGE:3596079) |
CNY 3,900.00 |
|
| MR209451L3 | Lenti ORF clone of Golga2 (Myc-DDK-tagged) - Mouse golgi autoantigen, golgin subfamily a, 2 (cDNA clone MGC:11816 IMAGE:3596079) |
CNY 5,420.00 |
|
| MR209451L4 | Lenti ORF clone of Golga2 (mGFP-tagged) - Mouse golgi autoantigen, golgin subfamily a, 2 (cDNA clone MGC:11816 IMAGE:3596079) |
CNY 5,420.00 |
