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Golga2 (BC011407) Mouse Untagged Clone
CAT＃: MC200685
Golga2 (untagged) - Mouse golgi autoantigen, golgin subfamily a, 2 (cDNA clone MGC:11816 IMAGE:3596079), (10ug)
CNY 2,000.00
货期*
3周
规格
Product images
经常一起买 (3)
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | GM130, golgin-95, GM-130, GM 130, mKIAA4150 |
| Vector | PCMV6-Kan/Neo |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>BC011407
AAGAAACCAGGCAGAGCAAATTGGCTGCGGCCAAGAAAAAGCTGAGAGAGTATCAGCAGAAGAACAGCCC GGGTGTTCCTGCAGGAGCAAAGAAGAAGAAAAAGATTAAAAATGGCCATAGCCCAGAGAGACCCACTGCC AGTGACTGTCAGTCACCAGAGAATAGCCATGATGCTGGCAATTGCTCTAACCTCATGGAGGAGACCAAGA CTTTCTCATCGACTGAGAGTCTGCGACAACTTTCTCAACAGCTCAATGGCCTTGTGTCTGAGTCTACATC CTACATCAACGGGGAGGGCCTCACATCTTCCAACATGAAGGAACTGGAGAACCGGTACCAAGAGCTAGCA GTAGCCCTGGACTCCAGCTATGTAACAAACAAACAACTCAGTAGCACGATAGAGGAATTGAAACAACAGA ACCAAGACACTCTGGATCAACTGGAGAAAGAGAAGAAGGATTATCAGCAGAAGCTGGCAAAGGAGCAGGG CTCTCTCAGGGAACAGCTGCAGGTTCACATTCAGACCATAGGCATCCTGGTGTCTGAGAAGGCAGAATTA CAGACGGCCCTGGCCCACACTCAGCAAGCAGCCAGGCAGAAAGCAGGAGAGTCGGAGGATCTTGCCAGCC GACTACAGTCCTCCCGACAGCGTGTGGGAGAGCTGGAGCGGACACTGTCTACCGTGTCAACGCAACAGAA ACAGGCAGACAGGTATAACAAGGACCTAACCAAAGAGCGGGATGCCCTCAAGCTGGAGCTGTATAAGAAC AGCAAAAGCAATGAGGACCTGAGGCAGCAGAACTCAGAACTGGAAGAGAAACTCCGAGTCCTGGTGGCAG AGAAAGCAGCTGCACAGTTGGGGGTGGAGGAGCTGCAGAAGAAGTTAGAGATGTCAGAGCTGCTGCTGCA GCAGTTTTCTAGTCAGTCTTCGGCCGCAGGCGGTAATGAGCAGTTACAACATGCCATGGAGGAGCGGGCT CAGCTGGAGACCCATGTTAGCCAGCTGATGGAATCACTGAAGCAGCTCCAGGTGGAGAGAGACCAGTATG CGGAGAACCTAAAAGGAGAGAGTGCCATGTGGCAGCAGAGGGTACAGCAAATGGCGGAGCAGGTGCATAC ACTGAAGGAAGAGAAGGAGCACAGAGAACGGCAGGTACAAGAGCTGGAGACCAGCTTGGCCGCACTCCGG AGTCAGATGGAGGAGCCACCGCCTCCAGAGCCTCCAGCTGGGCCCTCCGAGGCGGAAGAGCAGCTGCAGG GAGAGGTCGAGCAGCTGCACAAGGAACTGGAGAGGCTGACGGGACAGCTACGGGCTCAGGTGCAGGACAA TGAGAGCCTGAGCCACCTCAACCGGGAGCAGGAGGGGCGTCTGCTGGAGCTGGAGCGGGAGGCCCAGCGC TGGAGCGAGCAGGCCGAGGAGCGCAAGCAGATCCTGGAGAGCATGCAGAGTGACCGCACCACCATCAGCA GAGCACTGTCACAGAACCGAGAGCTGAAGGAGCAGCTGGCCGAGCTGCAGAACGGCTTCGTCAGGCTGAC CAATGAGAACATGGAGATCACCAGTGCGCTGCAGTCAGAGCAGCATGTCAAGAAAGAGCTAGCCCGGAAG CTGGGAGAGCTGCAGGAGAGACTGGGAGAGCTGAAGGAGACGGTGGAGCTCAAGAGCCAAGAAGCACAGG GTCTACAGGAGCAGCGAGACCAGTGCCTGTCCCACCTGCAGCAGTACGCTGCTGCTTACCAACAGCACTT GGCGGCCTATGAGCAGCTGACCTCTGAGAAGGAGGCAATACACAAGCAGCTTCTGCTGCAGACTCAGCTC ATGGACCAGCTGCAGCATGAGGAGGTACAGGGCAAGATGGCAGCCGAGCTGGCCCGCCAGGAGCTGCAGG AGGCCCAGGAGCGCCTAAAAGCTACCAGCCAGGAGAACCAACAGCTCCAAGCCCAGCTGAGCCTCTTGGT CCTCCCTGGGGAAGGAGATGTGGACCAGGAGGAAGAAGATGAGGAGGTTCCTCAGTCGAGTCTGGCCATC CCAGAAGACCTAGACAGTAGAGAGGCCATGGTGGCATTCTTTAATGCCGCTATAGCCAGAGCAGAGGAAG AACAGGCCCGACTACGTGTGCAGCTGAAAGAGCAGAAGGCACGTTGCCGGAGTTTGTCTCACTTGGCAGC CCCAGTCCAAAGCAAGCTTGAAAAGGAGGCAGTGGTCCCCAGGAATGTGGATGACTCTGCATCTGAGGAG AGCAATCAGGCCCTACATGTAGCCATGGAGAAGCTGCAGAGCCGCTTCCTGGAGGTCATGCAGGAGAAAG TGGAGCTCAAGGAGAGGGTAGAGGAGCTTGAACATTGCTGCATTCAGCTTTCTGGAGAGACAGACACCAT TGGAGAGTACATTGCCCTTTACCAAAATCAGAGGGCGGTGCTGAAGGCTCGGCATTTGGAGAAGGAAGAA TACATCAGCCGGCTGGCTCAGGACAAGGAGGAGATGAAGGTGAAGCTGCTGGAGCTCCAGGAGCTGGTGT TGAGGCTTGTGAACGAGCGCAATGAATGGCAGGGCAAGTTCCTGGCCGTCTCTCAGAACCCTGGTGACGT GCTCACCCCAGTGCCCACAGGCTCCCAGGAATTTGGTGCTGCTGACCAGCAGGATGACCTCAGGGAGGTG AGCCTGGCTGACGATATAGAGCCTGCACAAGGAGAGGCAGGGGTACCTGCTCCCCATGAGAACCCTACTG CACAGCAGATCATGCAGCTGCTGCGTGAGATCCAGAACCCCCGGGAGCGGCCAGGCCTGGGTAGCAACCC TTGCATCCCCTTTTTCTACCGTGCCGATGAGAACGACGAGGTGAAGATCATGGTTGTATAAAAGACTGGC GGCCAAAGCCTCAGCAAGGGCAGCCAGAGGTTCTGCCCCAACCCACACCCTTCCTCAGCCACCCTTATCT TTATAATAATAATAAGGTCAGACTCCATCCCCTCCAGGGGCTGGGGAAGTAGCTCAGTTGGTAGAGTGCT TGCCTGCTGTGCACACAGCCTTGGGTCCCCAGTACCTCTGAAAAGGCTGATACCTGTAATTTGAGTGCTT GGGAAGTGGAGGTGGAAGAATCAGCCATCCTCATCTACCTAGTGAGTTTGAGGCCACACTGGGCTACATG CGACCCTGTCTGAAAAAACCAAAAGATCAGGCTCCTATCTTAATGAGGGACACAGACTGGTACAGATCTG CCTCTTAGCCAGGGCAGTTTTACTTTGTAAATTCCTGTTACAGAAAGAACGCCAGAGACTCAGTGATGGA GCAGACTGTGCTGGGTGGCCTGGGGGCTTTCTCACCAAGGAGGGCCTCAGGCCTTACTCAGGACTCTGGG GCAGAACAGAGTGGCAGCATTCTCGTGCCCAGGTGCAGTGCAGGCTGGTAAACTGGCCTAGCATTCAGCA GGGCTTCTAAGTTTTCACCGGCTCAGTCCTCCCTATCCTCCTTTTCCTCCCCGCCCCCATTTTATCTTCA TTTTCTTTACGGAGTGTCTCCTGTAGCCCGAGCTGAGCCCCTGCCTCACCGCTGTGCCTGGTGGTTGGCA GTGCTAAGCAGGCACTCTACCAAGTGATTTACCCCCAGCACTCCCTGCCTTTTTCCTTTGTGTGGTGATG CTGGGGATGGAACCCAGGGCACAGCAGGTCCCCAGATCAACTCCCATCTTCTCAGAGGCACTTTAGGGCA GTGGGGCTGGGCAGCACTTCATGGGTCCTCAGGCAGTTGGGGCTAACTGCCTCAGGAAGGCATCCCACTT TGGAGGGCTTCCATCTTTTTGAGGCACTTTGGGACAGGGAAAGTGGGTACCATTCTCTCAGGCCTTATGA CAATTGGGGTAACTACGCCAAGCAGGACAGAGGCTGCTGGGGCAGGGTGGCCTTCCCCTCCCCCGGTGTA CATATTGTACCTGTGTACTATTTTGTATATACCGGGGTAGGGACACCCCTGTATCATAGTGGAGGTTGGA GCTGGCAAATGGGAAGAGCTTCTAATAATCACTTTGGGCTGGGAACCTTATTTATTGGTAGTGTTAGGTC AGAGGGCAGGAGGCGGAGACAAGGTTGTGGCACCTGCTGATGCAGCTCCTCTTTATTTTGCTTTTTACTT GGGAAATAAATGGATTTAGCCATAAAAAAAAAAAAAAAAAAAAA |
| Restriction Sites | RsrII-NotI |
| ACCN | BC011407 |
| Insert Size | 1854 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | BC011407, AAH11407 |
| RefSeq Size | 4174 bp |
| RefSeq ORF | 1854 bp |
| Locus ID | 99412 |
| Gene Summary | Peripheral membrane component of the cis-Golgi stack that acts as a membrane skeleton that maintains the structure of the Golgi apparatus, and as a vesicle thether that facilitates vesicle fusion to the Golgi membrane (PubMed:28028212). Required for normal protein transport from the endoplasmic reticulum to the Golgi apparatus and the cell membrane (PubMed:28028212). Together with p115/USO1 and STX5, involved in vesicle tethering and fusion at the cis-Golgi membrane to maintain the stacked and inter-connected structure of the Golgi apparatus. Plays a central role in mitotic Golgi disassembly: phosphorylation at Ser-37 by CDK1 at the onset of mitosis inhibits the interaction with p115/USO1, preventing tethering of COPI vesicles and thereby inhibiting transport through the Golgi apparatus during mitosis. Also plays a key role in spindle pole assembly and centrosome organization (By similarity). Promotes the mitotic spindle pole assembly by activating the spindle assembly factor TPX2 to nucleate microtubules around the Golgi and capture them to couple mitotic membranes to the spindle: upon phosphorylation at the onset of mitosis, GOLGA2 interacts with importin-alpha via the nuclear localization signal region, leading to recruit importin-alpha to the Golgi membranes and liberate the spindle assembly factor TPX2 from importin-alpha. TPX2 then activates AURKA kinase and stimulates local microtubule nucleation. Upon filament assembly, nascent microtubules are further captured by GOLGA2, thus linking Golgi membranes to the spindle (By similarity). Regulates the meiotic spindle pole assembly, probably via the same mechanism (PubMed:21552007). Also regulates the centrosome organization (By similarity). Also required for the Golgi ribbon formation and glycosylation of membrane and secretory proteins (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG209451 | Golga2 (tGFP-tagged) - Mouse golgi autoantigen, golgin subfamily a, 2 (cDNA clone MGC:11816 IMAGE:3596079) |
CNY 3,900.00 |
|
| MR209451 | Golga2 (Myc-DDK-tagged) - Mouse golgi autoantigen, golgin subfamily a, 2 (cDNA clone MGC:11816 IMAGE:3596079) |
CNY 4,592.00 |
|
| MR209451L3 | Lenti ORF clone of Golga2 (Myc-DDK-tagged) - Mouse golgi autoantigen, golgin subfamily a, 2 (cDNA clone MGC:11816 IMAGE:3596079) |
CNY 5,420.00 |
|
| MR209451L4 | Lenti ORF clone of Golga2 (mGFP-tagged) - Mouse golgi autoantigen, golgin subfamily a, 2 (cDNA clone MGC:11816 IMAGE:3596079) |
CNY 5,420.00 |
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