United States
Germany
Japan
United Kingdom
China
Tie1 (BC060182) Mouse Tagged Lenti ORF Clone
CAT＃: MR209734L3
- LentiORF®
Lenti ORF clone of Tie1 (Myc-DDK-tagged) - Mouse tyrosine kinase receptor 1 (cDNA clone MGC:63223 IMAGE:6831929)
"BC060182" in other vectors (4)
Need custom modification / cloning service?
Get a free quote
CNY 8,360.00
Product images
CNY 600.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | D430008P04Rik; TIE; tie-1 |
| Vector | pLenti-C-Myc-DDK-P2A-Puro |
| E. coli Selection | Chloramphenicol (34 ug/mL) |
| Mammalian Cell Selection | Puromycin |
| Sequence Data |
The ORF insert of this clone is exactly the same as(MR209734).
|
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC060182 |
| ORF Size | 1941 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC060182.1 |
| RefSeq Size | 4259 bp |
| RefSeq ORF | 1943 bp |
| Locus ID | 21846 |
| Gene Summary | Transmembrane tyrosine-protein kinase that may modulate TEK/TIE2 activity and contribute to the regulation of angiogenesis.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC218258 | Tie1 (untagged) - Mouse tyrosine kinase receptor 1 (cDNA clone MGC:63223 IMAGE:6831929), (10ug) |
CNY 6,940.00 |
|
| MG209734 | Tie1 (tGFP-tagged) - Mouse tyrosine kinase receptor 1 (cDNA clone MGC:63223 IMAGE:6831929) |
CNY 9,304.00 |
|
| MR209734 | Tie1 (Myc-DDK-tagged) - Mouse tyrosine kinase receptor 1 (cDNA clone MGC:63223 IMAGE:6831929) |
CNY 6,460.00 |
|
| MR209734L4 | Lenti ORF clone of Tie1 (mGFP-tagged) - Mouse tyrosine kinase receptor 1 (cDNA clone MGC:63223 IMAGE:6831929) |
CNY 8,360.00 |
