Srrm3 (NM_021403) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	2900083I11Rik; AB041661; SRm300-like; Srrm2l
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR209738 representing NM_021403 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCCTCCACCGTGAACAACGGGGCTACCGGCATGCCAGCTCCACCGGATGCTGCTAATGGCTTCCCGC AGCCCGGCGCCTCGTCCGGGTCTTGGCCGCGCGCGGAGGAGGAGCTGCGCGCCGCCGAGCCCGGCTTGGT GAAGCGCGCGCACCGGGAGATCCTGGACCACGAACGCAAGCGGCGCGTGGAGCTCAAGTGCATGGAGTTG CAGGAGATGATGGAGGAGCAGGGGTACTCAGAGGAGGAGATCCGGCAGAAGGTCGGGACCTTCCGGCAGA TGCTGATGGAGAAGGAAGGCGTACTGACCCGGGAGGACCGGCCTGGGGCCCACATCGTGGCAGAGACCCC GCGACGAATGGAGGGCTTGGAGCCGGGCCTGGAGTACCCACCCTTCGATGAGGACGACGGCCCGGTGGAT TGCGACTGCCCGGTCTCCTGCTACCGCGGGCACCGGGGGTACAGGACCAAGCACTGGTCCAGCAGCTCTG CATCGCCCCCTCCCAAGAAGAAGAAGAAAAAGAAAGGCAGTCATCGGAGGAGCCGCAAAAAGAGGAGACT AGAGTCAGAATGCAGCTGTGGGAGTGCTTCCCCCCTGCGCAAGAAGAAGAAGAATGTGAAGAAACACCGT CGAGATCGGTCTGATTCTGGGTCCCGGAGGAAGAGACGATACAGATCGAGAAGCCTCAAGAGCAAACGAA AAGAAAAAAACAAAGAGCGAAAGAGGCCTCACACTGAGTCTCCAGGCCGAAGGTTCCACCACCACAGCAG CGCCAGCTCCCACAGCCCCTCAATGTCCTCACACTACAGTGATTCTGGATCACCCAGCAGACTGAGCCCC AAGCATCGAGATGACGGGCGGAAGACAGGCAGCCAGCGATCCAGCGGGAGCCGGTCGCCATCCCCATCGG GCGGCAGCGGATGGGGGTCGCCCCAGCAGAACGGAGGCAGCAGGCAGCGAAGCGGAGCGCACGGGGGTCG CCCCGGCTCGGCGCACAGCCCGCCCGATAAGCCCAGCTCGCCCCGGGCCTGCGACAAGGCGGCCGCCGCG CCCACGCCTCCCGCGCGTGGGAAGGACAGCCAGAGTCCCCGCTCCGCGCCGTCGTCGCAGGGCCGCGGGG GCCGCGCGGCGGGAGGGACAGCCAGGCGCCGGCGTCGGCGACGGAGGAGGAGACGATCGCGGTCCTCGGC CAACGCGCCCCGCCGCAGGGGACGCCGGCGTACAAAGCCCGCGCCGCCTCGGGGCTCGTCGCGCTCGCTC AGCGGGGCACACTCCAGCAGCGACTCCGGTGGCGGCGCCCCGGGCCCCGGGCCCGAGCCCTGCTCCGAGC GAGGTCACGGCGGACACGGGAAACGGGCAAAGGAGCGCCCCCCGCGCGCACGCCCGGCTAGCACCTCGCC ATCGCCTGGCACGCGCGGCCGACGGGGCGGCCCAGAGGGCAACAGCTCATCGCGCAGCCCGGGTCCCCAT CAGGGCTCCTGGAGCTCCAGCCGCTCGCCCTCCAAATCCCACTCGCGCTCCCCAGACAAAAGGACCCGCA GCCCCAGCCTCTCACCGTCGCCCAAGAAACCTCTTGGCAGGGACAAGGACAGCGAAGGCCGTGCGAGGCA CGCCGAGGCTGAGGCCGCCCGCACCCGGCGTCGCTCCCGCAGCTACTCGCCCATCCGAAAGCGGCGCCGG GACTCGCCCAGTTTCATGGAGCCGAGACGCATCACCAGTGCCCGCAAGCGTCCCATCCCCTACTACCGGC CCAGCCCCTCCTCTTCCTCCAGCTGCTTGAGCACCGACTACTCAAGCCGGAGCCACAGCCGGAGCCCCAG CCCTGGCCACAGCCATGGAAGCTACAGCAGCCGCAGTCACGGGACTCGCAGCCGCTCCTGCAGCGCCTCC AGGAGCCGCAGTCCTAGCTACCACAGCCGGAGCAGCTCTGAGAGTGGGGGCTTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR209738 representing NM_021403 Red=Cloning site Green=Tags(s) MSSTVNNGATGMPAPPDAANGFPQPGASSGSWPRAEEELRAAEPGLVKRAHREILDHERKRRVELKCMEL QEMMEEQGYSEEEIRQKVGTFRQMLMEKEGVLTREDRPGAHIVAETPRRMEGLEPGLEYPPFDEDDGPVD CDCPVSCYRGHRGYRTKHWSSSSASPPPKKKKKKKGSHRRSRKKRRLESECSCGSASPLRKKKKNVKKHR RDRSDSGSRRKRRYRSRSLKSKRKEKNKERKRPHTESPGRRFHHHSSASSHSPSMSSHYSDSGSPSRLSP KHRDDGRKTGSQRSSGSRSPSPSGGSGWGSPQQNGGSRQRSGAHGGRPGSAHSPPDKPSSPRACDKAAAA PTPPARGKDSQSPRSAPSSQGRGGRAAGGTARRRRRRRRRRRSRSSANAPRRRGRRRTKPAPPRGSSRSL SGAHSSSDSGGGAPGPGPEPCSERGHGGHGKRAKERPPRARPASTSPSPGTRGRRGGPEGNSSSRSPGPH QGSWSSSRSPSKSHSRSPDKRTRSPSLSPSPKKPLGRDKDSEGRARHAEAEAARTRRRSRSYSPIRKRRR DSPSFMEPRRITSARKRPIPYYRPSPSSSSSCLSTDYSSRSHSRSPSPGHSHGSYSSRSHGTRSRSCSAS RSRSPSYHSRSSSESGGF TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms	CHROMATOGRAMS Sequencher program is needed, download here.
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_021403
ORF Size	1944 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_021403.3, NP_067378.2
RefSeq Size	3970 bp
RefSeq ORF	1947 bp
Locus ID	58212
UniProt ID	Q80WV7
MW	71.1 kDa
Gene Summary	May play a role in regulating breast cancer cell invasiveness. May be involved in RYBP-mediated breast cancer progression.[UniProtKB/Swiss-Prot Function]

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