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5730559C18Rik (NM_028872) Mouse Tagged ORF Clone
CAT＃: MR209867
- TrueORF®
5730559C18Rik (Myc-DDK-tagged) - Mouse RIKEN cDNA 5730559C18 gene (5730559C18Rik)
ORF Plasmid: tGFP
"NM_028872" in other vectors (4)
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CNY 4,944.00
CNY 5,800.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 1700034M08Rik; 4933426C09Rik; AI586180; D1Mgi54 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR209867 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTGCAAATGCCGAAGTTAAATGAAATACCTCCGGGGAGGGGAGGCCCGGGGGAGCCTTGGGGAGAGG GAAGATGGGCGGGACCAACAGGTCCTGAAGCTGCGCGACCGGCGCGGGGGGCGCGGGGGCAGGCGCGAGG CGCCAGAGCTCGGTGGGACAGCTGGGAACACTCCAGACTACCTACACATCCTGGCCCAGGCTGGGACCAG TGCAGCCCCTCCTTCCTCTGTGCCCCCTCTTCCCAGAAACTCATCATGGAGAGTAAGGATGAAGTCAGTG ACTCCGACAGCGGCATCATCCTGCAGTCTGGCCCGGACAGCCCTGTCTCTCCAATGAAAGAGCTGACCAA TGCAGTACGCAAGCAGCAGAGGGCTCTGGAAGCGAGGCTGGAGGCCTGCCTGGAGGAGCTGCGCAGACTC TGCCTGAGGGAGGCGGAGCTGACTGGGACTTTGCCGGCAGAGTATCCCCTTAAGCCTGGTGAGAAGGCCC CCAAGGTCCGGCGCAGGATCGGAGCAGCGTACAAGCTGGATGAGTGGGCCCTCCACAGAGAGGACCCCCT GAGCAGCCTAGAGCGCCAGTTGGCTCTGCAGCTTCAGATCACCGAGGCGGCTCGAAGGCTGTGTGCAGAG GAGAACCTCAGCAGACAGGCACGCAGGCAGCGGAAGCACGCTGCGCTGCAGGAGGAGAAGAAGCTGCGGG ACCTTCAGCGTTGCCTGGGTGATCGGCGGCGGAACAGTGAGCCCCCTCCTACCACTGTGCCCTCCCTGGG CAGAGAACTCAGTGCCTCTGACGACAGCTCCCTGTCTGATGGGCTGCTCCTGGAGGAAGAAGACTCCCAA GCGCCAAAACCTCCCCCAGAGTCACCGGCACCACCTTCGCGGCCTCTCCCACCCCAGAGCCTTGAGGGCC TACAGCCAACAGGACCTGAGTCTGGCGGCCAGGAACGGGCTCCCATCCAAAACAGTCCCTGGAAGGAGAC CAGCCTGGACCACCCCTATGAGAAGCCCAGGAAGTCTTCTGAGCTCAGTAGCGAGTCCAGCAGCCCGGCC ACCACTCCTCAGGACCAGCCCAACCCTTCCAGCCTGTGGGTGCTGGATGCTGCCTCTTACCACGTGGTCC CCATCCGAAATGTTCCCGGCCAGCGTCAGGGCCGCACCAGTGCCCCAGCCACCCCTGAGATGCAGGGCAG GAGGGGCCAGTCACAGTCTCTGAGGGTGGACTCCTTCCGAGCAGGCGCGGAGGGCCGAGGTCGCAGTGCC TTTCCCCGCCGCCGCCCCACTCATTACACAGTGACAGTGCCAGACTCCTGCTTCACACCCGGCAAGCCCC CACTGCCACACCCTGCCTGCCATTCCTGCTCTGAAGACAGTGGCTCTGATGTCTCCAGCATCTCCCATCC CACCTCACCTGGCAGCAGCAGTCCAGACATCTCCTTTCTGAGGCCCCTCTGTCTCCCTGAGCCACCTCGC CACCGTGGGGCCTGGGGCCCAGCCTGTGGCAGAGAGCTGGCCCCTCATTACTCTAAACTTCTGCTACCCG CTGGATACTTCCCAACGGGGCGCTATGTGATGGTGGCCGAGGGCCACCTGCCGCCGGGCGAGTGGGAGCT GTGTCGTGCAGCCGTGGGTGCTGCCTATGACGAGGAAGGTGCACCCCTGCGTTACCAGCGACTGGTACCC TCCCACAGCCGCATTGTGCGCACACCCTCACTAAAGGACAGTCCTGCGGGCCGGGGACTCAGCAAGGCAG CAGTCTCGGAGGAGCTCAAGTGGTGGCATGAACGCGCACGTCTCCGGAGCAGCCGCCCCCACTCGCTCGA CCGCCAAGGGGCGTTCCGTGTTAGGAGTCTGCCTCCTGGGAGGGAGAGCTTCGGGCGGGCCTCAGGACCC AGGACACAGGTGCCCCCCGTGTACGTACTCCGGAGGTCGACGGACGGGGCCCCAGTGCAAGTCTTTGTGC CTGAGAACGGAGAGATTATCAGCCAAGTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR209867 protein sequence
Red=Cloning site Green=Tags(s) MLQMPKLNEIPPGRGGPGEPWGEGRWAGPTGPEAARPARGARGQARGARARWDSWEHSRLPTHPGPGWDQ CSPSFLCAPSSQKLIMESKDEVSDSDSGIILQSGPDSPVSPMKELTNAVRKQQRALEARLEACLEELRRL CLREAELTGTLPAEYPLKPGEKAPKVRRRIGAAYKLDEWALHREDPLSSLERQLALQLQITEAARRLCAE ENLSRQARRQRKHAALQEEKKLRDLQRCLGDRRRNSEPPPTTVPSLGRELSASDDSSLSDGLLLEEEDSQ APKPPPESPAPPSRPLPPQSLEGLQPTGPESGGQERAPIQNSPWKETSLDHPYEKPRKSSELSSESSSPA TTPQDQPNPSSLWVLDAASYHVVPIRNVPGQRQGRTSAPATPEMQGRRGQSQSLRVDSFRAGAEGRGRSA FPRRRPTHYTVTVPDSCFTPGKPPLPHPACHSCSEDSGSDVSSISHPTSPGSSSPDISFLRPLCLPEPPR HRGAWGPACGRELAPHYSKLLLPAGYFPTGRYVMVAEGHLPPGEWELCRAAVGAAYDEEGAPLRYQRLVP SHSRIVRTPSLKDSPAGRGLSKAAVSEELKWWHERARLRSSRPHSLDRQGAFRVRSLPPGRESFGRASGP RTQVPPVYVLRRSTDGAPVQVFVPENGEIISQV TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_028872 |
| ORF Size | 1992 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_028872.3, NP_083148.3 |
| RefSeq Size | 3013 bp |
| RefSeq ORF | 2034 bp |
| Locus ID | 67313 |
| UniProt ID | Q7TN12 |
| MW | 72.7 kDa |
| Gene Summary | Expressed in peripheral macrophages and intestinal myeloid-derived cells, is required for optimal PRR (pattern recognition receptor)-induced signaling, cytokine secretion, and bacterial clearance. Upon stimulation of a broad range of PRRs (pattern recognition receptor) such as NOD2 or TLR2, TLR3, TLR4, TLR5, TLR7 and TLR9, associates with YWHAQ/14-3-3T, which in turn leads to the recruitment and activation of MAP kinases and NF-kappa-B signaling complexes that amplifies PRR-induced downstream signals and cytokine secretion (By similarity). In the intestine, regulates adherens junction stability by regulating the degradation of CYTH1 and CYTH2, probably acting as substrate cofactor for SCF E3 ubiquitin-protein ligase complexes. Stabilizes adherens junctions by limiting CYTH1-dependent ARF6 activation (PubMed:29420262).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC220258 | 5730559C18Rik (untagged) - Mouse RIKEN cDNA 5730559C18 gene (5730559C18Rik), (10ug) |
CNY 8,550.00 |
|
| MG209867 | 5730559C18Rik (tGFP-tagged) - Mouse RIKEN cDNA 5730559C18 gene (5730559C18Rik) |
CNY 6,370.00 |
|
| MR209867L3 | Lenti ORF clone of 5730559C18Rik (Myc-DDK-tagged) - Mouse RIKEN cDNA 5730559C18 gene (5730559C18Rik) |
CNY 7,700.00 |
|
| MR209867L4 | Lenti ORF clone of 5730559C18Rik (mGFP-tagged) - Mouse RIKEN cDNA 5730559C18 gene (5730559C18Rik) |
CNY 7,700.00 |
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