Cnot4 (NM_001164413) Mouse Tagged ORF Clone
CAT#: MR210197
- TrueORF®
Cnot4 (Myc-DDK-tagged) - Mouse CCR4-NOT transcription complex, subunit 4 (Cnot4), transcript variant 1
"NM_001164413" in other vectors (3)
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CNY 5,208.00
CNY 6,270.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | Not4; Not4h; Not4hp |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR210197 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCTCGCAGTCCTGATGCAAAGGAAGACCCTGTGGAATGCCCTCTTTGCATGGAGCCCTTGGAAATAG ATGATATTAACTTTTTCCCTTGCACCTGTGGCTACCAGATATGCCGATTTTGTTGGCATCGAATTCGCAC TGATGAGAATGGGCTTTGTCCTGCATGTAGAAAGCCATATCCAGAAGATCCAGCAGTTTACAAACCACTT TCCCAGGAAGAATTACAAAGGATAAAGAATGAGAAAAAACAGAAACAAAATGAAAGAAAACAGAAAATCT CAGAAAATAGAAAACATTTGGCTAGTGTACGTGTTGTACAAAAAAACCTTGTGTTTGTTGTAGGTCTGTC ACAGCGCCTAGCAGACCCAGAAGTTTTAAAACGACCAGAGTATTTTGGGAAGTTTGGTAAAATACATAAA GTTGTCATCAATAATAGCACATCCTATGCAGGCTCACAGGGTCCGAGTGCCAGTGCTTATGTAACGTATA TCCGGTCAGAAGATGCTCTCAGAGCAATACAGTGTGTCAACAATGTGGTAGTAGATGGCAGGACACTAAA GGCATCTTTAGGTACAACAAAGTACTGCAGTTATTTCTTAAAGAACATGCAGTGCCCAAAGCCTGACTGC ATGTATCTTCATGAATTGGGAGATGAGGCAGCCAGCTTCACAAAAGAGGAAATGCAGGCGGGTAAGCACC AAGAATATGAACAGAAGCTCCTCCAAGAATTATATAAATTAAATCCCAATTTTCTTCAGCTATCTACGGG TTCAGTTGATAAGAATAAGAACAAAGTGACACCATTACAAAGCCCCATTGACAAACCTTCAGATTCTCTC AGTATAGGGAATGGTGATAATTCCCAACAGATATCTAACAGTGATACGCCTTCACCACCACCTGGTTTAT CAAAATCCAACCCTGTCATCCCCATCAGTTCATCCAATCACAGTGCGAGGTCTCCTTTTGAAGGGGCAGT AACAGAGTCACAGTCATTATTCTCAGACAATTTTCGCCACCCCAACCCTATACCAAGTGGGCTTCCTCCT TTTCCCAGCTCTCCACAGACACCCAGTGATTGGCCTACAGCTCCAGAACCACAGAGCCTCTTCACATCAG AAACAATCCCAGTTTCATCATCTACAGACTGGCAAGCAGCATTCGGCTTTGGTTCTTCTAAACAACCAGA GGATGACTTGGGGTTTGACCCCTTTGATGTCACTCGAAAAGCCTTAGCAGACCTGATTGAGAAGGAACTA TCCGTCCAAGATCAACCTTCCCTTTCCCCCACATCTCTTCAGAACGCCTCCTCACACACTACGACCGCCA AAGGGCCAGGCTCTGGATTCCTGCACTCTGCTGCACCTACAAATGCCAACTCTCTCAATAGTACCTTTTC AGTCTTGCCACAGAGGTTCCCTCAATTTCAGCAGCACCGAGCAGTTTATAATTCGTTCGGTTTTCCAGGC CAGGCAGCCCGCTATCCTTGGATGGCCTTTCCACGCAATAGCATCATGCACTTGAACCACACAGCAAACC CCACCTCAAATAGTAATTTCTTGGACTTGAATCTCCCGCCACAGCACAACACAGGTCTGGGAGGGATCCC CATAGCAGACAACAACAGTTCTGTAGAGAGTTTAAATATGAAGGAATGGCAGGACGGGCTAAGGGCACTT CTACCCAACATTAACATCAACTTTGGTGGACTGCCCAATTCTTCTTCCCCCTCCAACGCCAACCACAGTG CACCAACGTCCAACACTGCCACCACGGACAGCGTGAGTTGGGACAGCCCTGGCAGCTGGACAGACCCAGC CATCATCACAGGTATTCCAGCATCTTCAGGAAACACTTTAGACTCTATTCAAGACGACAATCCTCCACAC TGGCTAAAATCCCTACAGGCCCTCACAGAGATGGACGGCCCCAGCGCTGCCTCGTCACAACCCCATCACA GCGCCCCCTTCAGCACACAGATCCCTCTGCACAGAGCCAGTTGGAACCCCTACCCACCTCCTTCAAATCC CTCCAGCTTCCACTCTCCACCCCCAGGCTTCCAGACAGCCTTCAGACCCCCCAGCAAAACCCCCACAGAT CTGCTACAGAGTTCCACACTGGACCGCCAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR210197 protein sequence
Red=Cloning site Green=Tags(s) MSRSPDAKEDPVECPLCMEPLEIDDINFFPCTCGYQICRFCWHRIRTDENGLCPACRKPYPEDPAVYKPL SQEELQRIKNEKKQKQNERKQKISENRKHLASVRVVQKNLVFVVGLSQRLADPEVLKRPEYFGKFGKIHK VVINNSTSYAGSQGPSASAYVTYIRSEDALRAIQCVNNVVVDGRTLKASLGTTKYCSYFLKNMQCPKPDC MYLHELGDEAASFTKEEMQAGKHQEYEQKLLQELYKLNPNFLQLSTGSVDKNKNKVTPLQSPIDKPSDSL SIGNGDNSQQISNSDTPSPPPGLSKSNPVIPISSSNHSARSPFEGAVTESQSLFSDNFRHPNPIPSGLPP FPSSPQTPSDWPTAPEPQSLFTSETIPVSSSTDWQAAFGFGSSKQPEDDLGFDPFDVTRKALADLIEKEL SVQDQPSLSPTSLQNASSHTTTAKGPGSGFLHSAAPTNANSLNSTFSVLPQRFPQFQQHRAVYNSFGFPG QAARYPWMAFPRNSIMHLNHTANPTSNSNFLDLNLPPQHNTGLGGIPIADNNSSVESLNMKEWQDGLRAL LPNININFGGLPNSSSPSNANHSAPTSNTATTDSVSWDSPGSWTDPAIITGIPASSGNTLDSIQDDNPPH WLKSLQALTEMDGPSAASSQPHHSAPFSTQIPLHRASWNPYPPPSNPSSFHSPPPGFQTAFRPPSKTPTD LLQSSTLDRH TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001164413 |
ORF Size | 2133 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001164413.1, NP_001157885.1 |
RefSeq Size | 3548 bp |
RefSeq ORF | 2133 bp |
Locus ID | 53621 |
UniProt ID | Q8BT14 |
MW | 77.8 kDa |
Gene Summary | Has E3 ubiquitin ligase activity, promoting ubiquitination and degradation of target proteins. Involved in activation of the JAK/STAT pathway. Catalyzes ubiquitination of methylated RBM15.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC220789 | Cnot4 (untagged) - Mouse CCR4-NOT transcription complex, subunit 4 (Cnot4), transcript variant 1, (10ug) |
CNY 9,120.00 |
|
MR210197L3 | Lenti ORF clone of Cnot4 (Myc-DDK-tagged) - Mouse CCR4-NOT transcription complex, subunit 4 (Cnot4), transcript variant 1 |
CNY 8,170.00 |
|
MR210197L4 | Lenti ORF clone of Cnot4 (mGFP-tagged) - Mouse CCR4-NOT transcription complex, subunit 4 (Cnot4), transcript variant 1 |
CNY 8,170.00 |