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Tagap (NM_145968) Mouse Tagged ORF Clone
CAT＃: MR210217
- TrueORF®
Tagap (Myc-DDK-tagged) - Mouse T-cell activation Rho GTPase-activating protein (Tagap)
ORF Plasmid: tGFP
"NM_145968" in other vectors (4)
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CNY 5,232.00
CNY 6,270.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 2610315E15Rik |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR210217 representing NM_145968
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGCTGATAAGCAGTCTCGATGGTTCAAAAACACTTAATGCTAACAACATGGAGACATTAATTGAAT GTCAGTCAGAGGGTGATATCAAAGTGCCTCCCCTGCTGACATCATGTGAGAGTGAAGACAGCATTTGCCA GCTAACTGAAATTAAGAAGAGAAAGAAAGTGCTGTCCTGGCCATCTCTCATGAGAAAGCTCTCTCCTTCA TCCGACTTCTCTGGGTCATTGGAACCAGAGCTGAAAGTGTCGCTGTTTGACCAACCCTTGTCGATCATCT GTGGGGAGAACGACACACTTCCCAGACCCATCCAGGACATCCTCACCATCCTCTGCCTTAAAGGTCCTTC AACGGAAGGGATATTCAGGAAAGCTGCCAGCGAGAAAGCCCGCAAGGAGCTGAAGGAGGGGCTTAACTGT GGGGTCTCCGTGAATCTGAAGCAGCTCCCTGTGCACCTCCTAGCTGTGGTCTTCAAGGACTTCCTCCGAG GGATACCCCTGAAGCTACTCTCCTGTGATCTCTTTGAGGACTGGATGGGCGCCCTGGAGAAGCCCACCGA GGAGGACAGAATCGAGGCCCTGAAGCAGGTTGCTGGTGGCCTCCCCCGGCCCAACCTTCTCCTGCTCAGG CACTTGCTCTACGTGCTCCACCTCATCAGCAAGAACGCTGAGGTCAACAAGATGGACTCCAGCAACCTCG CCATCTGCATCGGGCCTAACATGCTCACTCTGAAGAACGACCAGAGCCTGTCCTTCCAGGCCCAGAAGGA CCTGAACAATAAGGTTAAGATCTTGGTGGAATTCCTCATCGACAACTGCTTTGAGATATTTGGGGAGAAC ATTCGGACGCGTTCCCGCATCACTTCTGACGACTCCCTGGAACACACTGACAGTTCAGACGTGTCAACTC TGCAGAATGACTCGGCCTATGACAGCAATGACCCAGATGTGGAGCCCACAAGTGGCGCAGCCTCTCCCAA CAGGCAACTGGAGGGTCCCACTCCCACAATGGCTGGTCTGGATACCCGGGGCCAACGGGACACCTGTGAG TCAAGCTCAGAGTCCAGCGTTAGCATGGTAGTCAGGCTGAAAAGCTCCATTGTCCAACAAGACAGGCGGT TCTCTGAACCCAACATGTCACCCTCACGAGAGTGCCTCGTGGGCCCAACATCCAAGCAAAAGCTAGCAAG GAGTGAGGACAGCTTCACTCTGTCCCAGGACGCCTCCTGTTCTGAAGGCGATGAAGCTGAAGATCCCTTT ACAGAGGAAGTCTTCCCAGCAGTGGACAGCAAACCCAAGAGACCTGTGGATTTGAAAATAAAGAATTGGA CCCAAGGTTTAGCATCTCCACAGGGACACATAACCAAAGCTTTCTCCAGATCCTCCCCAGGCGAATCTTT GGGCAGCTCACCTGTGCCTTCTCCATCCTGCCCCAAGAGAAACTTCTTCACCAGACACCAGAGTTTCACA ACAAAGACAGACAAAACCAAACCCCAGAGAGAAATTAGAAAGCACTCCATGTCGTTTTCCTTTGCGTCTC ACAAGAAAGTGCTGCCCCGAACCTCCAGCATTGGGTCTGAGAAATCCAAAGACTTTTCTAGAGACCAACT CCAGAAGGACTTGAGGAAAGAGAGCCAACTTTCTGGCAGAATCGTCCAGGAAAATGAGTCAGAAATCCAA AGCCAAACATCTCTGGGCTTCAGCTTGTCTGGCACCTGGGCCCTCTCAGTCGACAACACGTTCCAGTTAG TGGATATGAGGAAACCAGGAAGCCCACCATCTTACGAAGAGGCCATTTATTACCAGACATCAGGACTCAC AGCCTACGGTGGCCAGACAGTTGGGAGTATGAGGTCAAGAATGTTCAAGCCAAGCACAGCAGTGCCCCCT GTGCCTTCTCACCATGGAGGTGACCTCAGTGAAGGGACACCTGGTGGACACAGATTGTCTTCTGTGACTG AGCACTGGACACACAGTCAGACTGTCCATGTCTCTATAGAAACTCAGGGGAGATCTGAGCTACACCAGTT GAGGACAGTGTCCGAGTCCATGCAGAAGGCTAAGCTGGACTGTCTTGGGCCACAACACAGCCACTTAGTC TTTGAGGCTGACCAACTCTGCTGTGCTAGAGAATCCTACATT AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA >MR210217 representing NM_145968
Red=Cloning site Green=Tags(s) MKLISSLDGSKTLNANNMETLIECQSEGDIKVPPLLTSCESEDSICQLTEIKKRKKVLSWPSLMRKLSPS SDFSGSLEPELKVSLFDQPLSIICGENDTLPRPIQDILTILCLKGPSTEGIFRKAASEKARKELKEGLNC GVSVNLKQLPVHLLAVVFKDFLRGIPLKLLSCDLFEDWMGALEKPTEEDRIEALKQVAGGLPRPNLLLLR HLLYVLHLISKNAEVNKMDSSNLAICIGPNMLTLKNDQSLSFQAQKDLNNKVKILVEFLIDNCFEIFGEN IRTRSRITSDDSLEHTDSSDVSTLQNDSAYDSNDPDVEPTSGAASPNRQLEGPTPTMAGLDTRGQRDTCE SSSESSVSMVVRLKSSIVQQDRRFSEPNMSPSRECLVGPTSKQKLARSEDSFTLSQDASCSEGDEAEDPF TEEVFPAVDSKPKRPVDLKIKNWTQGLASPQGHITKAFSRSSPGESLGSSPVPSPSCPKRNFFTRHQSFT TKTDKTKPQREIRKHSMSFSFASHKKVLPRTSSIGSEKSKDFSRDQLQKDLRKESQLSGRIVQENESEIQ SQTSLGFSLSGTWALSVDNTFQLVDMRKPGSPPSYEEAIYYQTSGLTAYGGQTVGSMRSRMFKPSTAVPP VPSHHGGDLSEGTPGGHRLSSVTEHWTHSQTVHVSIETQGRSELHQLRTVSESMQKAKLDCLGPQHSHLV FEADQLCCARESYI SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_145968 |
| ORF Size | 2142 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_145968.2, NP_666080.1 |
| RefSeq Size | 3076 bp |
| RefSeq ORF | 2145 bp |
| Locus ID | 72536 |
| UniProt ID | B2RWW0 |
| MW | 78.9 kDa |
| Gene Summary | May function as a GTPase-activating protein. May play a role in transmission ratio distortion (TRD) in mouse, in which heterozygous males for t-locus transmit their t-carrying chromosome to 95% or more of their offspring.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC221016 | Tagap (untagged) - Mouse T-cell activation Rho GTPase-activating protein (Tagap), (10ug) |
CNY 9,220.00 |
|
| MG210217 | Tagap (tGFP-tagged) - Mouse T-cell activation Rho GTPase-activating protein (Tagap) |
CNY 6,940.00 |
|
| MR210217L3 | Lenti ORF clone of Tagap (Myc-DDK-tagged) - Mouse T-cell activation Rho GTPase-activating protein (Tagap) |
CNY 8,170.00 |
|
| MR210217L4 | Lenti ORF clone of Tagap (mGFP-tagged) - Mouse T-cell activation Rho GTPase-activating protein (Tagap) |
CNY 8,170.00 |
