Agbl5 (BC057349) Mouse Tagged ORF Clone

CAT#: MR210236

  • TrueORF®

Agbl5 (Myc-DDK-tagged) - Mouse ATP/GTP binding protein-like 5 (cDNA clone MGC:67138 IMAGE:6813901)

ORF Plasmid: DDK tGFP



  "BC057349" in other vectors (4)

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Mouse Protein / Lysate Request

CNY 5,248.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Synonyms 4930455N08; 9430057O19Rik; CCP5
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR210236 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAACATGAACAAGCAAAGCAAACTGTATTCCCAGGGCATGGCCCCCTTTGTGCGCACACTGCCTTCCC
GGCCACGCTGGGAACGCATTAGAGAGCGGCCCACCTTTGAGATGACAGAGACACAGTTTGTGCTATCCTT
TGTTCACCGTTTCGTAGAGGGCCGGGGAGCCACCACCTTCTTCGCCTTCTGCTACCCCTTCTCCTACAGT
GACTGCCAGGATCTGCTGAGCCAGCTAGACCAGCGCTTTTCAGAGAATTACTCTACCCATAGCAGTCCTC
TGGATAGTATTTATTACCACCGAGAGCTTCTCTGCTATTCTCTGGATGGACTTCGTGTAGATCTACTAAC
AATCACTTCCTGCCATGGGCTTCGAGATGATCGGGAGCCCCGACTAGAGCAGCTATTTCCTGATCTCGGC
ACTCCCCGACCATTCCGTTTCACAGGCAAAAGGATATTCTTCTTAAGCAGTAGGGTACACCCTGGAGAGA
CTCCATCTAGCTTTGTGTTCAATGGCTTTCTGGACTTCATCCTTCGACCTGATGATCCCCGGGCACAAAC
TCTCCGTCGACTCTTTGTTTTTAAGTTGATTCCCATGTTAAACCCTGATGGTGTGGTCCGGGGCCACTAC
CGCACGGACTCCCGTGGAGTGAATCTGAACCGTCAGTACTTGAAGCCTGATGCCGTCCTGCACCCGGCCA
TCTATGGCGCCAAAGCTGTGCTGCTTTATCATCACGTGCACTCGAGGCTCAACGCCAAGAGTCCCACGAA
CCAGCAACCCACTCTCCATCTCCCTCCGGAAGCCCCTCTCTCTGACCTCGAGAAAGCCAATAATCTCCAC
AATGAAGCTCACCTCGGGCAGTCACCTGATGGGGAAAACCCTGCGACTTGGCCTGAGACAGAGCCAGCAG
AAGAGAAGACTGACCCTGTGTGGCTTATGCCACAGCCAATTCCTGAACTCGAGGAGCCAGCCCCTGACAC
CATCCCCCCAAAAGAGAGTGGTGTTGCTTACTATGTAGACTTGCACGGACATGCTTCCAAAAGGGGCTGC
TTCATGTATGGCAACAGCTTTAGTGACGAGAGCACCCAGGTGGAAAACATGCTATATCCAAAGCTCATCT
CCTTGAATTCAGCCCACTTTGACTTCCAGGGCTGCAATTTCTCAGAGAAGAACATGTATGCCCGAGACCG
TAGAGATGGCCAGTCCAAAGAGGGAAGCGGCCGTGTCGCAATCTACAAAGCCTCAGGGATAATCCACAGC
TACACACTTGAATGCAACTACAACACTGGACGCTCAGTCAACAGCATCCCTGCTGCCTGCCATGACAACG
GGCGCGCCAGCCCCCCTCCCCCTCCGGCTTTCCCCTCCAGATACACTGTGGAACTATTCGAGCAGGTGGG
GCGAGCTATGGCCATCGCAGCTCTGGACATGGCAGAGTGTAATCCGTGGCCCCGAATTGTGCTGTCGGAA
CACAGCAGCCTCACCAACCTGCGAGCCTGGATGCTGAGGCACGTGCGCAACAGCAGAGGGCTGACCAGTG
CTGGGAACATGGGCGCGAGCAAGAAGCGAGGGGCTCGAACCCCTCCCAAAAGCAACAACAGCTTGCCTGT
TTCCTGCTCTGAAAATGCTCTGAGCCGGGTTCGCAGTTTTAGCACCGGCACGAGTACTGGTGGCAGCAGC
AGCAGCCAACAAAACTCTCCACAGATGAAGAACTCTCCCAGCTTCCCTTTTCATGGCAGTCGGACTGCGG
GGCTACCAGGCCTGGGCTCTAGCACCCAAAAGGTCAGCCATCGGGTGCTGGGCCCTGTCAGAGAGCCCCG
ATGCTCAGACAGAAGGCGGCGACAGCAGCCACTGAACCATCGCTCTACCACAAGCAGCCTGGCCCCATCC
CCAACTCTGGCTAGTTCTGGCCCAACCTCCTCACGAAACATGGGCTCCTGCCTCCTACCCAATTCACTCA
GCTTATCAGGGAGCAGCTGCTCATTCTCGTCTTCAGGGGACAAGCCAGAGGCTGTCATGGTGATTGGGAA
AAGCCTACTAGGGGCTGGTGCTCGGATCCCTTGCATTAGGACTCGATTGCAGACTCCATTGTCCCTGCCA
GATGCACCCCAACCACAGCCTACTAGCTTTGGCTTTCCTACCCGTCCT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR210236 protein sequence
Red=Cloning site Green=Tags(s)

MNMNKQSKLYSQGMAPFVRTLPSRPRWERIRERPTFEMTETQFVLSFVHRFVEGRGATTFFAFCYPFSYS
DCQDLLSQLDQRFSENYSTHSSPLDSIYYHRELLCYSLDGLRVDLLTITSCHGLRDDREPRLEQLFPDLG
TPRPFRFTGKRIFFLSSRVHPGETPSSFVFNGFLDFILRPDDPRAQTLRRLFVFKLIPMLNPDGVVRGHY
RTDSRGVNLNRQYLKPDAVLHPAIYGAKAVLLYHHVHSRLNAKSPTNQQPTLHLPPEAPLSDLEKANNLH
NEAHLGQSPDGENPATWPETEPAEEKTDPVWLMPQPIPELEEPAPDTIPPKESGVAYYVDLHGHASKRGC
FMYGNSFSDESTQVENMLYPKLISLNSAHFDFQGCNFSEKNMYARDRRDGQSKEGSGRVAIYKASGIIHS
YTLECNYNTGRSVNSIPAACHDNGRASPPPPPAFPSRYTVELFEQVGRAMAIAALDMAECNPWPRIVLSE
HSSLTNLRAWMLRHVRNSRGLTSAGNMGASKKRGARTPPKSNNSLPVSCSENALSRVRSFSTGTSTGGSS
SSQQNSPQMKNSPSFPFHGSRTAGLPGLGSSTQKVSHRVLGPVREPRCSDRRRRQQPLNHRSTTSSLAPS
PTLASSGPTSSRNMGSCLLPNSLSLSGSSCSFSSSGDKPEAVMVIGKSLLGAGARIPCIRTRLQTPLSLP
DAPQPQPTSFGFPTRP

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC057349
ORF Size 2148 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC057349, AAH57349
RefSeq Size 3332 bp
RefSeq ORF 2150 bp
Locus ID 231093
MW 79.5 kDa
Gene Summary Metallocarboxypeptidase that mediates protein deglutamylation. Specifically catalyzes the deglutamylation of the branching point glutamate side chains generated by post-translational glutamylation in proteins such as tubulins (PubMed:20519502, PubMed:21074048). In contrast, it is not able to act as a long-chain deglutamylase that shortens long polyglutamate chains, a process catalyzed by AGTPBP1/CCP1, AGBL2/CCP2, AGBL3/CCP3, AGBL1/CCP4 and AGBL4/CCP6 (PubMed:25103237). Mediates deglutamylation of CGAS, regulating the antiviral activity of CGAS (PubMed:26829768).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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