Pcca (NM_144844) Mouse Tagged ORF Clone
CAT#: MR210302
- TrueORF®
Pcca (Myc-DDK-tagged) - Mouse propionyl-Coenzyme A carboxylase, alpha polypeptide (Pcca)
ORF Plasmid: tGFP
"NM_144844" in other vectors (4)
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CNY 5,304.00
CNY 5,800.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | C79630 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR210302 representing NM_144844
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGGGCAGTGGGTCAGGACCGTGGCGCTGTTGGCGGCCAGGCGGCATTGGCGGCGGTCCTCGCAGC AGCAATTGCTGGGGACGCTGAAGCATGCTCCAGTCTATTCATACCAATGCCTAGTGGTGTCCAGAAGTCT CAGTTCTGTGGAATATGAGCCTAAAGAAAAGACTTTTGATAAAATTCTCATTGCTAACAGAGGAGAAATT GCCTGTAGGGTTATTAAAACTTGCAAGAAGATGGGCATCAAGACAGTTGCCATTCACAGTGATGTTGATG CCAGTTCTGTTCACGTGAAAATGGCGGATGAGGCTGTCTGTGTTGGCCCAGCTCCCACCAGTAAAAGCTA CCTCAACATGGATGCCATCATGGAAGCCATTAAGAAAACCAGGGCCCAAGCTGTACACCCAGGGTATGGA TTCCTGTCAGAAAACAAAGAGTTTGCAAAGCGTCTGGCAGCAGAAGATGTCACTTTCATTGGACCTGATA CTCATGCTATTCAAGCCATGGGTGACAAGATAGAAAGCAAACTATTAGCCAAGAGAGCAAAGGTCAACAC AATCCCTGGTTTTGATGGGGTAGTAAAGGATGCAGATGAAGCTGTCAGAATTGCAAGGGAAATTGGCTAC CCTGTGATGATCAAGGCCTCAGCAGGCGGTGGTGGGAAAGGCATGCGCATCGCCTGGGATGACGAAGAGA CCAGGGATGGCTTTAGATTTTCATCCCAGGAAGCTGCTTCTAGTTTTGGTGATGATAGACTACTAATAGA AAAATTTATCGATAACCCTCGTCATATAGAAATCCAGGTTTTAGGGGATAAACATGGCAATGCTCTGTGG CTCAATGAGAGGGAGTGCTCGATCCAGAGAAGGAACCAGAAGGTGGTAGAGGAGGCGCCAAGCATTTTTC TGGATCCTGAAACTCGCCAAGCAATGGGAGAGCAGGCCGTGGCTTTGGCTAAAGCCGTGAAGTATTCTTC TGCTGGAACTGTGGAATTTCTTGTGGACTCCCAGAAGAATTTTTACTTCTTGGAGATGAATACAAGACTA CAGGTCGAACATCCTGTCACAGAGTGCATTACTGGCCTGGACTTAGTCCAAGAAATGATCCTTGTTGCTA AGGGTTACCCACTCAGGCACAAGCAAGAGGATATTCCCATCAGTGGCTGGGCAGTTGAATGTCGGGTTTA TGCTGAGGACCCCTACAAGTCTTTCGGTTTACCGTCTATTGGGAGGCTGTCCCAGTACCAAGAGCCGATA CATCTACCTGGTGTCCGAGTTGACAGTGGCATCCAACCAGGAAGTGACATCAGCATCTATTATGATCCTA TGATTTCAAAGCTAGTCACATATGGGTCTGACAGAGCAGAAGCCCTGAAGAGGATGGAAGACGCACTGGA CAATTATGTGATCCGGGGTGTTACACACAACATCCCATTGCTCCGGGAGGTGATAATCAACACACGTTTT GTGAAAGGAGACATCAGCACTAAGTTTCTCTCTGATGTGTATCCTGATGGCTTCAAAGGGCACACGTTAA CACTGAGTGAGAGAAACCAGTTATTGGCCATTGCATCATCTGTATTTGTGGCATCCCAGCTACGAGCTCA GCGCTTCCAAGAACATTCAAGAGTACCAGTTATTAGGCCTGATGTGGCTAAGTGGGAGCTCTCGGTAAAG TTACATGATGAAGATCATACTGTCGTGGCATCTAACAATGGGCCGGCATTTACCGTGGAAGTTGATGGCT CGAAACTAAATGTGACCAGTACGTGGAACCTGGCGTCACCCTTATTGTCTGTCAACGTTGATGGCACGCA GAGGACTGTGCAGTGTCTTTCTCGGGAAGCAGGTGGAAACATGAGCATCCAGTTTCTTGGCACAGTGTAC AAAGTGCACATTTTAACCAAGCTTGCTGCAGAGCTGAACAAATTCATGCTTGAAAAAGTGCCCAAGGACA CCAGCAGCACTCTGTGCTCCCCGATGCCTGGAGTGGTGGTGGCCGTTTCTGTCAAGCCTGGAGACATGGT AGCAGAAGGTCAGGAAATCTGTGTGATTGAAGCTATGAAAATGCAGAACAGTATGACAGCTGGGAAAATG GGCAAGGTGAAATTGGTGCACTGCAAAGCTGGAGACACAGTTGGTGAAGGAGACCTGCTTGTGGAGCTGG AA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR210302 representing NM_144844
Red=Cloning site Green=Tags(s) MAGQWVRTVALLAARRHWRRSSQQQLLGTLKHAPVYSYQCLVVSRSLSSVEYEPKEKTFDKILIANRGEI ACRVIKTCKKMGIKTVAIHSDVDASSVHVKMADEAVCVGPAPTSKSYLNMDAIMEAIKKTRAQAVHPGYG FLSENKEFAKRLAAEDVTFIGPDTHAIQAMGDKIESKLLAKRAKVNTIPGFDGVVKDADEAVRIAREIGY PVMIKASAGGGGKGMRIAWDDEETRDGFRFSSQEAASSFGDDRLLIEKFIDNPRHIEIQVLGDKHGNALW LNERECSIQRRNQKVVEEAPSIFLDPETRQAMGEQAVALAKAVKYSSAGTVEFLVDSQKNFYFLEMNTRL QVEHPVTECITGLDLVQEMILVAKGYPLRHKQEDIPISGWAVECRVYAEDPYKSFGLPSIGRLSQYQEPI HLPGVRVDSGIQPGSDISIYYDPMISKLVTYGSDRAEALKRMEDALDNYVIRGVTHNIPLLREVIINTRF VKGDISTKFLSDVYPDGFKGHTLTLSERNQLLAIASSVFVASQLRAQRFQEHSRVPVIRPDVAKWELSVK LHDEDHTVVASNNGPAFTVEVDGSKLNVTSTWNLASPLLSVNVDGTQRTVQCLSREAGGNMSIQFLGTVY KVHILTKLAAELNKFMLEKVPKDTSSTLCSPMPGVVVAVSVKPGDMVAEGQEICVIEAMKMQNSMTAGKM GKVKLVHCKAGDTVGEGDLLVELE TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_144844 |
ORF Size | 2172 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_144844.2, NP_659093.2 |
RefSeq Size | 2603 bp |
RefSeq ORF | 2175 bp |
Locus ID | 110821 |
UniProt ID | Q91ZA3 |
MW | 80.4 kDa |
Gene Summary | This is one of the 2 subunits of the biotin-dependent propionyl-CoA carboxylase (PCC), a mitochondrial enzyme involved in the catabolism of odd chain fatty acids, branched-chain amino acids isoleucine, threonine, methionine, and valine and other metabolites. Propionyl-CoA carboxylase catalyzes the carboxylation of propionyl-CoA/propanoyl-CoA to D-methylmalonyl-CoA/(S)-methylmalonyl-CoA (By similarity). Within the holoenzyme, the alpha subunit catalyzes the ATP-dependent carboxylation of the biotin carried by the biotin carboxyl carrier (BCC) domain, while the beta subunit then transfers the carboxyl group from carboxylated biotin to propionyl-CoA (By similarity). Propionyl-CoA carboxylase also significantly acts on butyryl-CoA/butanoyl-CoA, which is converted to ethylmalonyl-CoA/(2S)-ethylmalonyl-CoA (By similarity). Other alternative minor substrates include (2E)-butenoyl-CoA/crotonoyl-CoA (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC200415 | Pcca (untagged) - Mouse propionyl-Coenzyme A carboxylase, alpha polypeptide (Pcca), (10ug) |
CNY 5,312.00 |
|
MG210302 | Pcca (tGFP-tagged) - Mouse propionyl-Coenzyme A carboxylase, alpha polypeptide (Pcca) |
CNY 6,370.00 |
|
MR210302L3 | Lenti ORF clone of Pcca (Myc-DDK-tagged) - Mouse propionyl-Coenzyme A carboxylase, alpha polypeptide (Pcca) |
CNY 7,700.00 |
|
MR210302L4 | Lenti ORF clone of Pcca (mGFP-tagged) - Mouse propionyl-Coenzyme A carboxylase, alpha polypeptide (Pcca) |
CNY 7,700.00 |