Cul2 (BC026779) Mouse Tagged ORF Clone

CAT#: MR210430

  • TrueORF®

Cul2 (Myc-DDK-tagged) - Mouse cullin 2 (cDNA clone MGC:25783 IMAGE:4018298)

ORF Plasmid: DDK tGFP



  "BC026779" in other vectors (4)

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Mouse Protein / Lysate Request

CNY 5,456.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


Goat Anti-CUL2 Antibody
    • 100 ug

CNY 5,371.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Synonyms 1300003D18Rik; 4932411N15Rik; AI327301; mKIAA4106
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR210430 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCTTTAAAACCAAGAGTAGTGGATTTTGATGAGACATGGAACAAACTCCTAACTACAATCAAAGCTG
TTGTTATGTTGGAATATGTCGAGCGAGCAACATGGAATGACCGCTTCTCAGATATCTATGCTTTATGTGT
GGCCTATCCTGAACCACTTGGAGAAAGACTTTATGCGGAAACAAAGATTTTTTTGGAAAGTCACGTTCGG
CATTTGTATAAGAGAGTTCTGGAGTCTGAAGAACAAGTGCTTGTTATGTATCATAGGTACTGGGAAGAAT
ACAGCAAAGGCGCCGACTATATGGACTGCTTATATAGGTATCTCAATACCCAGTATATTAAAAAGAATAA
ATTGACAGAAGCTGATATACAGTATGGCTATGGTGGAGTAGATATGAATGAACCACTTATGGAAATAGGA
GAGCTAGCATTGGATATGTGGAGGAAGTTAATGGTTGAACCCCTTCAGAACATCCTCATCCGGATGCTGC
TTCGAGAAATTAAAAATGATCGTGGTGGTGAAGATCCAAACCAGAAAGTAATCCATGGGGTTATTAACTC
CTTTGTTCATGTTGAACAGTATAAGAAAAAATTTCCCTTAAAGTTTTATCAGGGAATATTCGTGTCTCCC
TTTCTGACTGAAACGGGAGAATATTACAAGCAAGAAGCTTCAAATTTATTACAAGAATCAAATTGTTCAC
AGTATATGGAAAAGGTTCTAGGTAGATTAAAAGATGAAGAAATTAGATGTCGAAAATATTTGCATCCAAG
TTCATATACTAAAGTGATTCATGAGTGTCAGCAGCGGATGGTAGCAGACCACTTACAGTTCTTACATTCA
GAGTGCCACAGCATCATTCAGCAAGAGAGGAAGAACGACATGGCAAATATGTACGTCTTACTCCGTGCTG
TGTCCAGTGGTTTACCTCATATGATTGAGGAGCTGCAGAAACATATCCATGACGAGGGCCTCAGAGCGAC
CAGTAACCTTACTCAGGAACATATGCCAACACTATTTGTGGAATCAGTTTTGGAAGTACATGGTAAATTC
GTTCAACTTATCAACACTGTTTTGAATGGTGATCAGCATTTTATGAGTGCTTTAGATAAGGCCCTTACAT
CTGTTGTAAATTACAGAGAACCCAAGTCTGTTTGCAAAGCGCCTGAACTGCTTGCTAAGTACTGTGACAA
CTTGCTAAAGAAGTCAGCGAAGGGGATGACTGAAAATGAAGTGGAGGACAAGCTCACTAGCTTCATCACA
GTTTTCAAGTACATTGATGACAAGGACGTCTTTCAGAAGTTCTATGCAAGAATGCTAGCAAAACGTCTAA
TTCACGGGTTGTCTATGTCTATGGATTCTGAAGAAGCCATGATCAATAAATTAAAGCAAGCCTGTGGTTA
TGAGTTTACCAGCAAGCTGCATCGAATGTATACAGACATGAGTGTCAGCGCTGATTTGAACAATAAGTTC
AACAATTTCATCAGAAACCAAGACACCGTGATTGACTTGGGGATTAGTTTCCAAATATATGTTCTTCAGG
CTGGTGCGTGGCCTCTCACTCAGGCTCCTTCATCTACATTTGCAATTCCCCAAGAGCTAGAGAAAAGTGT
ACAGATGTTTGAATTGTTTTATAGCCAGCATTTCAGTGGACGGAAGCTGACATGGTTGCATTATCTCTGT
ACAGGTGAAGTTAAAATGAACTATTTGGGAAAACCATACGTAGCTATGGTTACAACCTACCAAATGGCAG
TTCTTCTTGCCTTTAACAACAGTGAAACTGTCAGCTACAAAGAACTTCAAGACAGTACCCAGATGAATGA
AAAGGAGCTGACCAAAACAATCAAATCATTACTTGATGTGAAAATGATTAACCATGATTCAGAAAAGGAA
GATATCGATGCAGAATCTTCATTTTCATTAAATATGAGCTTCAGCAGTAAGAGAACAAAATTTAAGATTA
CTACGTCAATGCAGAAGGATACGCCACAAGAACTGGAACAGACTAGAAGTGCTGTAGATGAGGACCGGAA
AATGTATCTTCAAGCTGCTATTGTCCGCATCATGAAAGCACGAAAAGTGCTTCGGCACAACGCCCTCATT
CAAGAGGTAATCAGCCAGTCAAGAGCCAGGTTCAATCCCAGTATCAGCATGATTAAGAAGTGCATAGAAG
TTCTGATAGACAAACAGTACATCGAGCGCAGCCAAGCATCTGCAGATGAATACAGTTACGTCGCA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR210430 protein sequence
Red=Cloning site Green=Tags(s)

MSLKPRVVDFDETWNKLLTTIKAVVMLEYVERATWNDRFSDIYALCVAYPEPLGERLYAETKIFLESHVR
HLYKRVLESEEQVLVMYHRYWEEYSKGADYMDCLYRYLNTQYIKKNKLTEADIQYGYGGVDMNEPLMEIG
ELALDMWRKLMVEPLQNILIRMLLREIKNDRGGEDPNQKVIHGVINSFVHVEQYKKKFPLKFYQGIFVSP
FLTETGEYYKQEASNLLQESNCSQYMEKVLGRLKDEEIRCRKYLHPSSYTKVIHECQQRMVADHLQFLHS
ECHSIIQQERKNDMANMYVLLRAVSSGLPHMIEELQKHIHDEGLRATSNLTQEHMPTLFVESVLEVHGKF
VQLINTVLNGDQHFMSALDKALTSVVNYREPKSVCKAPELLAKYCDNLLKKSAKGMTENEVEDKLTSFIT
VFKYIDDKDVFQKFYARMLAKRLIHGLSMSMDSEEAMINKLKQACGYEFTSKLHRMYTDMSVSADLNNKF
NNFIRNQDTVIDLGISFQIYVLQAGAWPLTQAPSSTFAIPQELEKSVQMFELFYSQHFSGRKLTWLHYLC
TGEVKMNYLGKPYVAMVTTYQMAVLLAFNNSETVSYKELQDSTQMNEKELTKTIKSLLDVKMINHDSEKE
DIDAESSFSLNMSFSSKRTKFKITTSMQKDTPQELEQTRSAVDEDRKMYLQAAIVRIMKARKVLRHNALI
QEVISQSRARFNPSISMIKKCIEVLIDKQYIERSQASADEYSYVA

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC026779
ORF Size 2235 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC026779, AAH26779
RefSeq Size 2765 bp
RefSeq ORF 2237 bp
Locus ID 71745
MW 86.9 kDa
Gene Summary Core component of multiple cullin-RING-based ECS (ElonginB/C-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination of target proteins. ECS complexes and ARIH1 collaborate in tandem to mediate ubiquitination of target proteins (By similarity). May serve as a rigid scaffold in the complex and may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and is inhibited by the association of the deneddylated cullin subunit with TIP120A/CAND1 (By similarity). The functional specificity of the ECS complex depends on the substrate recognition component. ECS(VHL) mediates the ubiquitination of hypoxia-inducible factor (HIF) (By similarity).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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