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Dact2 (NM_172826) Mouse Tagged ORF Clone
CAT＃: MR210495
- TrueORF®
Dact2 (Myc-DDK-tagged) - Mouse dapper homolog 2, antagonist of beta-catenin (xenopus) (Dact2)
ORF Plasmid: tGFP
"NM_172826" in other vectors (4)
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CNY 5,544.00
CNY 6,650.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 2900084M21Rik; A630024E20; dapper2; Dpr2; Frd2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR210495 representing NM_172826
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTGGGCACCGAGCGGCCAGGGGCCCGCGGGTTGGGACCGCCGCAGGGTGGGCGCGCGACTACGAGCCG CACTGGCCGGGCTGCAGGAGCTTCAGGGGCTGCGAGCCACGCAGCAAGCACGCGTGCGGGGAGCTCTGGG CCTGCACCCTGCGCCAGGGCCCCGCGGCCAGGAGCTGCGTCTGGAGGCGGCGCTGACCGCGCTACGGGAG CAGCTGTCACGGCTAAGGAGACAGGATGCTGGCCTGAAGACACACTTGGACCAGCTGGATCAGCAGATAA GTGAACTACAGCTGGATGTGAGCAGGTCTTCTTGCGAGGCCTTGGATAGTGACAGCAGACCCAGCTCAGG TTTCTATGAGCTGAGCGATGCTGGTTCCTGTTCTCTGTCCACCTCCTGCGCATCTGTTTGCAGCGACCGT CTATCCCCCTCCCTGGGTAGCTGGCTGCCTGTGTTCCAGCCCTCCAAGTCCAGGTCTGGCATCGGGGACT GGCGACCACGCTCTGCTGATGAGACCACTGTCCCTGCATGGAGCCCACAGCTCACAGAAGATAGCAGGCT CCTGCATGGTGCAGAGGGCACAGGCCGGCTGACGGGCATGTTCCGGCCCAGACCAGTATCTACAGGTGAT CTCGAAAGAGTCCTTCCAGCTGACGTGGGGCTCCAGAGAGCTGGTACTGATGCTGCACATCTCCTGGGCC AGGGGATAGAGATCCCAGCCCACGCCCTGGACCCCACGTACCAGCGTGACCTGGTGGCCAGGGGAGGCCA GGAGGTGTACCCATATCCCAGCCCCCTCCATGCGGTGGCCCTGCAGAGTCCCCTCTTCGCTCTGCCCAAA GAAGCCCCGTGTTTTGACATCTGCTCACCTCCCCAGGAGCCTCCTCTGGTCCCTGTTGATGAGAACAGGA CTCAACCTGAGCCGATCCGTGAGCTGGGCTCAGCCGAAGCCTACATCCACAGGCTGTTGCATCTGCGGGG CCAAGAGCTCCCCCTGAGAGATGTGGGGCAGGAGCAGGGAGGTGACACAGCTGCTTTTCCACCGAAGCCC TGTGGCCAGAGGTCGGAGAGCACATGTCAGCTGGAGAAGCAGGCCTGTGGAGCTGACAGGGGAGGACTGA AACTAGGTAGGGGTGCTGCCAAGGACAGCCTCAAGCAACATGGGCCTGTGTCCCTTGTGGGTGCTGAGCC CCTCAGCAGCCCCCTAAAGGAAGAAACCATTCCTTGGAATCCCTGTGTCCATGGAGACAATACTGTTGGT TCCTCACCCTGCTCCCAGGCCCAACAGCCTCTTAATGACTGTGGCCAAGGACCAGTCCTGTCACCATCCA GGGTGTTGGGCACTGAGAGCCCACCTCTGGCCCCGGAGCCCTTTGCCTATACATCCTGCACCACTGGTGA AACCTCTCCCGTGAAGCTGAGGATGGGCTTTTCCCAAAACAAGGCCGTGAAGGTCAGAAGAAGAGTTAGT GAGAAAGTCCCGAGGCTAGGGAAGCAGCTCCCTCCACAACCAGAGAGGCAGCGGGTCACAGAGCGGGACC CCTCCAGGCCCCATCAGGGAGGTCTCAGCAGGAGGCCCACACTGGCCCGGGAGCCTCCTGGACGCTCCTG CTCTGAGTCCACCCTCTACCCCGTGCCCTTCCTTGTCCCCGTAGTGGTGGCCCAGAGGGAAAGTTACCCA ACGTCACCCCAAGCGTTCTTCCCAATGGAGGCAGCTCTCCTCAGCTCAGCAGCCAGGCGGAAGCAGCGCA GGTGGCAGTCCACCATGGAGATCTCAGCCAAAGCCGGTTCAGTCAGCCAACCTGGGCCCAGCATGGGGCT CCCTAGGTCCCCAGCCAAGAGAGGAAGTGGTCCCAGGGCCCAGAGTAGGCCCACACTTGCCCGTCAGGAT GCCTGTGCGAGGTGTGAGTCGGACCCCTCGGAGCACTCTGCAGACTGCACCTCACTCTACCACTCCACCA TTGCCGAGACCAGCGAGGACGAGGAGGCGAGTGACCACACTGCCAACCGCTTTGGGGACGAGTCCAGCAG CAACGATTCAGAAGGGTGTTTCCGGGGCAGCCGCCGTCGCCTGGCAATAGGCAGTGCAGAGGCTGGGCAA GGGGGGTGGGCCTGGCCTCGGGTGCCCCCCCAGCAGCCCTCACGGGCCCCAGGAAACACCAGGCCACCCT TGCCCCCTGTGCCCAAACTGTGCCGCATCAAGGCCTCCAAGGCCCTGAAGAAGAAGATCCGAAGGTTTCA GCCAGCAGCCCTGAAGGTCATGACCATGGTG AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA >MR210495 representing NM_172826
Red=Cloning site Green=Tags(s) MWAPSGQGPAGWDRRRVGARLRAALAGLQELQGLRATQQARVRGALGLHPAPGPRGQELRLEAALTALRE QLSRLRRQDAGLKTHLDQLDQQISELQLDVSRSSCEALDSDSRPSSGFYELSDAGSCSLSTSCASVCSDR LSPSLGSWLPVFQPSKSRSGIGDWRPRSADETTVPAWSPQLTEDSRLLHGAEGTGRLTGMFRPRPVSTGD LERVLPADVGLQRAGTDAAHLLGQGIEIPAHALDPTYQRDLVARGGQEVYPYPSPLHAVALQSPLFALPK EAPCFDICSPPQEPPLVPVDENRTQPEPIRELGSAEAYIHRLLHLRGQELPLRDVGQEQGGDTAAFPPKP CGQRSESTCQLEKQACGADRGGLKLGRGAAKDSLKQHGPVSLVGAEPLSSPLKEETIPWNPCVHGDNTVG SSPCSQAQQPLNDCGQGPVLSPSRVLGTESPPLAPEPFAYTSCTTGETSPVKLRMGFSQNKAVKVRRRVS EKVPRLGKQLPPQPERQRVTERDPSRPHQGGLSRRPTLAREPPGRSCSESTLYPVPFLVPVVVAQRESYP TSPQAFFPMEAALLSSAARRKQRRWQSTMEISAKAGSVSQPGPSMGLPRSPAKRGSGPRAQSRPTLARQD ACARCESDPSEHSADCTSLYHSTIAETSEDEEASDHTANRFGDESSSNDSEGCFRGSRRRLAIGSAEAGQ GGWAWPRVPPQQPSRAPGNTRPPLPPVPKLCRIKASKALKKKIRRFQPAALKVMTMV SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_172826 |
| ORF Size | 2271 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_172826.3, NP_766414.3 |
| RefSeq Size | 2849 bp |
| RefSeq ORF | 2274 bp |
| Locus ID | 240025 |
| UniProt ID | Q7TN08 |
| MW | 82.1 kDa |
| Gene Summary | Involved in regulation of intracellular signaling pathways during development. Negatively regulates the Nodal signaling pathway, possibly by promoting the lysosomal degradation of Nodal receptors, such as TGFBR1. May be involved in control of the morphogenetic behavior of kidney ureteric bud cells by keeping cells epithelial and restraining their mesenchymal character. May play an inhibitory role in the re-epithelialization of skin wounds by attenuating TGF-beta signaling.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC205939 | Dact2 (untagged) - Mouse dapper homolog 2, antagonist of beta-catenin (xenopus) (Dact2), (10ug) |
CNY 5,552.00 |
|
| MG210495 | Dact2 (tGFP-tagged) - Mouse dapper homolog 2, antagonist of beta-catenin (xenopus) (Dact2) |
CNY 7,320.00 |
|
| MR210495L3 | Lenti ORF clone of Dact2 (Myc-DDK-tagged) - Mouse dapper homolog 2, antagonist of beta-catenin (xenopus) (Dact2) |
CNY 8,550.00 |
|
| MR210495L4 | Lenti ORF clone of Dact2 (mGFP-tagged) - Mouse dapper homolog 2, antagonist of beta-catenin (xenopus) (Dact2) |
CNY 8,550.00 |
