Pla2g4b (BC098210) Mouse Tagged ORF Clone

CAT#: MR210623

  • TrueORF®

Pla2g4b (Myc-DDK-tagged) - Mouse phospholipase A2, group IVB (cytosolic) (cDNA clone MGC:106779 IMAGE:6826157)

ORF Plasmid: DDK tGFP



  "BC098210" in other vectors (4)

Need custom modification / cloning service?
Get a free quote

Mouse Protein / Lysate Request

CNY 7,790.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Synonyms MGC28892
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR210623 representing BC098210
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCAGGCAAAGGTGCCAGAGACATGCCTGCTCACTGTACGCGTCCTCCGGGCCAGTGGCTTGCCTTCCA
AGGACCTAGTAACCTCCTCTGACTGCTATGTGACTCTGAACCTGCCCACGGCTTCCAGCCACACGCTCCA
GACACGCACAGTCAAGAACAGCCGAAACCCTGTCTGGAATCAGAACTTCCACTTCCGGATCCATAGGCAG
CTCAAGAATGTTATTGAACTGAAAGCCTTTGACCATGACCTGGTGACCAGAGATGACCCAGTATTGTCAG
TGCTGTTTGACGTGGGGACCCTGCAAATTGGGACTCAGCGCCAGAGCTTCTCCTTGGGTACTCAGGAGAA
AGGGTGCTTAGAAGTTGAGTTTCGGCTACAGACTCTGACAGACTGTGAGGAACAGCTCATCAGCAATGGC
ATCGTGGTGGCCCGGGAACTCTCCTGCTTGCACGTTGAACTGAAGAGGACAGGAGATCCAAAGAGGTCAG
AGCGCAAAGTTCAACTTGTGGTTGCTGGGGCCTGTGAGGGCCCACAGGATGCCTCTGCGGGCACTGGATC
TTTCCACTTCCATTATCCAGCCTGCTGGGAGCAAGAGCTGAATGTTCATCTGCAGGATGACCCCCATGAG
CAGCTGAAGGTGCCACTACGAACTCTACCCTCCTCGCAGCTGGTGAGACTTGTCTTCCCCACGTCCCAGG
AGCCACTGATGAGGCTGGAACTCAAGAAGGAAGAAGGACCAAAGGAGCTGGCTGTGCGGCTGGGCTGTGG
GCCCTGCCCTGAGGAACAGGCCTTCCTAAGCAAGAGGAAGCAGGTGGTGGCTGCCGCGCTGAAAAAGGCC
TTACAGCTGGACCAAGACCTGCACGAGGATGAGATCCCCGTGATTGCCGTGATGGCCACTGGTGGTGGGA
TCCGGGCCATGACGTCTTTGTATGGGCAGCTGGCCGGCCTGCAGGAGCTCGGCCTTCTCGACTGCATCTC
CTATATCACGGGGGCTTCAGGATCCACCTGGGCACTGGCCAACCTCTATGAGGACCCAGAGTGGTCGCAG
AAGGACCTGGCAGGGCCCACTGAGGTGCTGAAGACACAGGTGACAAAGAGCAAGCTGGGTGCGTTGGCCC
CTAGCCAACTTTGGCGGTACCGGCAAGAGCTGGCTGAGCGTGCTCGCCTGGGCCACCCAACCTGCTTCAC
CAACTTGTGGGCCCTCATTAATGAGGCCTTGCTGCACGACAAGCCCCATGAACACAAACTCTCAGATCAA
CGAGAGGCCCTGAGTCGAGGCCAGAACCCTCTGCCTATCTATTGTGCCCTCAACAGCAAGGAGCAGGGCC
TGAGTACCTTTGACTTTGGGGAGTGGTGTGAGTTCTCTCCATATGAAGTCGGCTTCCCCAAGTATGGAGC
CTTCATCTCCTCCGAGCTCTTTGGCTCGGAGTTCTTTATGGGGCGGCTGGTGAAGCAGCTTCCCGAGTCC
CGCATCTGCTTCCTGGAAGGCATCTGGAGCAATCTGTTTGCGGCCAGCCTCCAAGACAGCTTGTACTGGT
CCTCAGAACCCAGCCAGTTCTGGGACCGCTGGGCGCAGGATCAGGCCAACCTGGACAAAGAGCAGGTCCC
CCATCTGAAGATAGCAGAGCCACCGACAATGGCTGGCAGGATCGCCGAGCTCTTCACTGACCTCCTGACA
AAGCGTCCCCTCGCCCACGCCACCCACAACTTCACGCGAGGCCTCCATTTCCACAAGGACTATTTCCAGA
ACTCTCACTTCTCTGCCTGGAAAGCTTCCAAACTGGACAGGCTCCCCAACCAGCTGACACCCACTGAGCC
CCACCTCTGCCTCCTGGATGTTGGCTATCTCATCAATACTAGCTGCCCACCCCTCCTGCAGCCAACACGG
GATGTGGACCTCATCTTGTCACTGGACTACAACCTCTACGGAGCCTTTCAGCAGCTGCAGCTTCTGAGCC
GGTTCTGCCAGGAGCAGGGCATCCCTTTCCCATCCATCTCACCCAGCCCCGAGGAGCAGCGGCAGCCTCA
GGAGTGCCATCTGTTCTGTGACCCAGCCCAGCCCGAAGCCCCGGCTGTGCTGCACTTCCCTTTGGTCAAT
GACTCCTTCCAAGACTACTCTGCCCCTGGGGTGCCACGGACATCGGAGGAGAAGGCAGCTGGGGAGGTGA
ATCTGTCTTCTTCTGACTCCCCATACCACTACACAAAAGTGACCTACAGCCAGGAAGATGTGGACAAGCT
GTTACGGCTGACGCATTACAACATCTGCAACAACCAGGATCGGCTGCGGGAGGCCATGCACCAGGCCGTG
CAGCGGCGGAGAAAGCGCAAACAGTTTAGGCCGGAG


AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
TGGATTACAAGGATGACGACGATAAG
GTTTAA
>MR210623 representing BC098210
Red=Cloning site Green=Tags(s)

MQAKVPETCLLTVRVLRASGLPSKDLVTSSDCYVTLNLPTASSHTLQTRTVKNSRNPVWNQNFHFRIHRQ
LKNVIELKAFDHDLVTRDDPVLSVLFDVGTLQIGTQRQSFSLGTQEKGCLEVEFRLQTLTDCEEQLISNG
IVVARELSCLHVELKRTGDPKRSERKVQLVVAGACEGPQDASAGTGSFHFHYPACWEQELNVHLQDDPHE
QLKVPLRTLPSSQLVRLVFPTSQEPLMRLELKKEEGPKELAVRLGCGPCPEEQAFLSKRKQVVAAALKKA
LQLDQDLHEDEIPVIAVMATGGGIRAMTSLYGQLAGLQELGLLDCISYITGASGSTWALANLYEDPEWSQ
KDLAGPTEVLKTQVTKSKLGALAPSQLWRYRQELAERARLGHPTCFTNLWALINEALLHDKPHEHKLSDQ
REALSRGQNPLPIYCALNSKEQGLSTFDFGEWCEFSPYEVGFPKYGAFISSELFGSEFFMGRLVKQLPES
RICFLEGIWSNLFAASLQDSLYWSSEPSQFWDRWAQDQANLDKEQVPHLKIAEPPTMAGRIAELFTDLLT
KRPLAHATHNFTRGLHFHKDYFQNSHFSAWKASKLDRLPNQLTPTEPHLCLLDVGYLINTSCPPLLQPTR
DVDLILSLDYNLYGAFQQLQLLSRFCQEQGIPFPSISPSPEEQRQPQECHLFCDPAQPEAPAVLHFPLVN
DSFQDYSAPGVPRTSEEKAAGEVNLSSSDSPYHYTKVTYSQEDVDKLLRLTHYNICNNQDRLREAMHQAV
QRRRKRKQFRPE

SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-RsrII      Cloning Scheme for this gene      Plasmid Map     
ACCN BC098210
ORF Size 2346 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC098210
RefSeq Size 4272 bp
RefSeq ORF 2348 bp
Locus ID 211429
MW 156.6 kDa
Gene Summary Calcium-dependent phospholipase A2 that selectively hydrolyzes glycerophospholipids in the sn-2 position with a preference for arachidonoyl phospholipids. Has a much weaker activity than PLA2G4A (By similarity).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Other Versions

Customer Reviews 
Loading...