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Vps8 (BC032214) Mouse Tagged ORF Clone
CAT＃: MR210699
- TrueORF®
Vps8 (Myc-DDK-tagged) - Mouse vacuolar protein sorting 8 homolog (S. cerevisiae) (cDNA clone MGC:38232 IMAGE:5323693)
ORF Plasmid: tGFP
"BC032214" in other vectors (4)
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CNY 5,816.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | mKIAA0804, MGC38232 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR210699 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGGACTTAATTGTTCATTTCCAAGATAAAAAACTGCTGGAGAATGTGGAAGCCCTCATTGTCCACA TGGACATCACCAGCCTCGACATTCAGCAGGTGGTCCTCATGTGTTGGGAGAATCGTCTGTATGACGCCAT GGTCTATGTCTACAACCGAGGCATGAATGAGTTTATCAGTCCGATGGAGAAGCTTTTCAAAGTCATCGCC CCACCTCTGAATGCAGGGAAGACTCTTACAGATGAGCAAGTTGTTATGGGCAACAAGCTTCTGGTGTACA TCAGCTGTTGCCTGGCAGGTCGTGCCTACCCTCTCGGTGACATTCCTGAAGATCTTGTTCCCTTGGTTAA AAACCAGGTTTTTGAGTTTCTGATTCGCCTGCATTCTGTAGAAGCATCCTCCGAGGAAGAAGTCTATCCT TATGTTCGGACTTTGCTACATTTTGACACAAGAGAATTTCTGAATGTGCTGGCACTGACCTTTGAAGACT TTAAAAATGATAAGCAAGCTGTGGAATACCAGCAGCGCATTGTGGATATTTTATTGAAGGTTATGGTGGA GAATTCAGATTTTACCCCGTCACAAGTGGGGTGTCTCTTTACGTTCCTCGCTCGTCAGCTCGCAAAGCCT GACAATACCTTGTTTGTGAACAGGACCCTCTTTGATCAAGTCCTCGAGTTCCTCTGTAGCCCCGACGATG ACTCCCGCCACTCTGAAAGACAGCAGGTCCTTTTAGAATTGCTGCAGGCCGGAGGCATAGTTCAATTTGA AGAGAGTCGGCTCATCCGCATGGCAGAAAAGGCAGAATTCTATCAAATCTGTGAATTTATGTATGAGCGG GAACACCAGTATGACAAGATCATTGACTGCTACCTGCACGACCCATTGAGAGAGGAAGAAGTCTTCAATT ATATCCACAACATCTTATCTATTCCTGGTCACAGTGCTGAGGAGAAGCAGTCTGTGTGGCAGAAAGCAAT GAATCATATGGAGGAACTTGTGTCCCTGAAGCCCTGCAAAGCCGCAGAGCTGGTGGCTACTCACTTTTCT GAGCAGATCGAAGTGGTCATTGGACAGCTTCAGAACCAGCTTTTGCTTTTCAAATTTTTGAGGAGTCTTC TTGATCCAAGGGAAGGTGTTCATGTCAATCAGGAGTTGCTGCAGATCCCGCCTCACATTACAGAGCAGTT CATTGAGCTGCTGTGCCAGTTCAGTCCTGACCAAGTCATACAGACACTGCAGGTCCTTGAGTGCTACCGT CTGGAGGAAACGATTCAGATTACACAAAAGTATCAACTCCATGAAGTCACTGCGTATCTGCTGGAGAAGA AAGGGGATGCACACGGCGCCTTCCTGCTGCTGCTGGAGAGACTGCAGAGCAGGCTGCAAGAGATGACACG GCAGGACGAAAATACCAAGGAGGACATCCTGCTGAAAGGTGTTGAAGATACCATGGTAGAGACAATTGCT CTTTGCCAGAGAAATTCACAGAATTTGAATCAGCAGCAACGAGAGGCTCTATGGTTTCCACTGTTGGAGG CAATGATGACACCACAGAAGCTGTCCAGCTCGGCTGCTGCTCCTCATCCGCACTGTGAAGCTCTGAAGTC TTTGACCATGCAAGTCCTAAACAGCATGGCAGCGTTCATCGCCCTCCCGTCCATCCTACAGAGAATCTTG CAGGACCCAATTTATGGAAAAGGAAAGCTTGGAGAAATCCAGGGCCTTATTCTGGGGATGCTGGACACCT TCAACTATGAGCAAACCTTGCTAGAAACAACAGCCAGCCTCCTGAACCAAGATCTCCATTGGTCACTGTG TAACCTGAGAGCATCAGTATCCAGAGGACTCAATCCCAAGCAGGATTACTGTTCTATATGTTTACAGCAG TACAAAAGACGCCAAGAAATGGCTGATGAAATTATTGTCTTTAGCTGTGGCCATTTGTATCATTCATTCT GTCTCCAAAGTAAGGAATGCACCCTAGAGGTTGAGGGTCAGACGAGATGGGCGTGTCACAAATGCAGCTC AAGTAATAAAGCGGGCAAACTCAGTGAAAATCCTTCTGAAAACAAGAAAGGACGGATAACCTCGTCTCAG GTAAAAATGTCGCCCTCGTATCATCAGTCCAAAGGGGATCCTCCCGCCAGGAAGGCAAACTCAGAACCTG TTCTGGACCCACAGCAAATGCAAGCCTTTGATCAGCTCTGCCGTCTCTACAGAGGAAGTTCTAGGTTGGC TCTCCTTACGGAGCTCTCCCAGAACCGAGGTGGCGACAGCTGCAGGCCGTTTGCTGGCCCCCAGAGTGGG CCTGCTTTCAACAGCGTCTTCCAGAAGGAGAACTTCCAGCTGCAGCTCGCGCCTCCGCCTGTGGCTGAAG AC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR210699 protein sequence
Red=Cloning site Green=Tags(s) MKDLIVHFQDKKLLENVEALIVHMDITSLDIQQVVLMCWENRLYDAMVYVYNRGMNEFISPMEKLFKVIA PPLNAGKTLTDEQVVMGNKLLVYISCCLAGRAYPLGDIPEDLVPLVKNQVFEFLIRLHSVEASSEEEVYP YVRTLLHFDTREFLNVLALTFEDFKNDKQAVEYQQRIVDILLKVMVENSDFTPSQVGCLFTFLARQLAKP DNTLFVNRTLFDQVLEFLCSPDDDSRHSERQQVLLELLQAGGIVQFEESRLIRMAEKAEFYQICEFMYER EHQYDKIIDCYLHDPLREEEVFNYIHNILSIPGHSAEEKQSVWQKAMNHMEELVSLKPCKAAELVATHFS EQIEVVIGQLQNQLLLFKFLRSLLDPREGVHVNQELLQIPPHITEQFIELLCQFSPDQVIQTLQVLECYR LEETIQITQKYQLHEVTAYLLEKKGDAHGAFLLLLERLQSRLQEMTRQDENTKEDILLKGVEDTMVETIA LCQRNSQNLNQQQREALWFPLLEAMMTPQKLSSSAAAPHPHCEALKSLTMQVLNSMAAFIALPSILQRIL QDPIYGKGKLGEIQGLILGMLDTFNYEQTLLETTASLLNQDLHWSLCNLRASVSRGLNPKQDYCSICLQQ YKRRQEMADEIIVFSCGHLYHSFCLQSKECTLEVEGQTRWACHKCSSSNKAGKLSENPSENKKGRITSSQ VKMSPSYHQSKGDPPARKANSEPVLDPQQMQAFDQLCRLYRGSSRLALLTELSQNRGGDSCRPFAGPQSG PAFNSVFQKENFQLQLAPPPVAED TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC032214 |
| ORF Size | 2382 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC032214, AAH32214 |
| RefSeq Size | 3023 bp |
| RefSeq ORF | 2384 bp |
| Locus ID | 209018 |
| MW | 91 kDa |
| Gene Summary | Plays a role in vesicle-mediated protein trafficking of the endocytic membrane transport pathway. Believed to act as a component of the putative CORVET endosomal tethering complexes which is proposed to be involved in the Rab5-to-Rab7 endosome conversion probably implicating MON1A/B, and via binding SNAREs and SNARE complexes to mediate tethering and docking events during SNARE-mediated membrane fusion. The CORVET complex is proposed to function as a Rab5 effector to mediate early endosome fusion probably in specific endosome subpopulations. Functions predominantly in APPL1-containing endosomes (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC220673 | Vps8 (untagged) - Mouse vacuolar protein sorting 8 homolog (S. cerevisiae) (cDNA clone MGC:38232 IMAGE:5323693), (10ug) |
CNY 9,120.00 |
|
| MG210699 | Vps8 (tGFP-tagged) - Mouse vacuolar protein sorting 8 homolog (S. cerevisiae) (cDNA clone MGC:38232 IMAGE:5323693) |
CNY 5,040.00 |
|
| MR210699L3 | Lenti ORF clone of Vps8 (Myc-DDK-tagged) - Mouse vacuolar protein sorting 8 homolog (S. cerevisiae) (cDNA clone MGC:38232 IMAGE:5323693) |
CNY 6,460.00 |
|
| MR210699L4 | Lenti ORF clone of Vps8 (mGFP-tagged) - Mouse vacuolar protein sorting 8 homolog (S. cerevisiae) (cDNA clone MGC:38232 IMAGE:5323693) |
CNY 6,460.00 |
