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Suv420h1 (BC075709) Mouse Tagged ORF Clone
CAT＃: MR210794
- TrueORF®
Suv420h1 (Myc-DDK-tagged) - Mouse suppressor of variegation 4-20 homolog 1 (Drosophila) (cDNA clone MGC:78059 IMAGE:3661032)
ORF Plasmid: tGFP
"BC075709" in other vectors (4)
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CNY 8,170.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | MGC18702, Suv4-20h1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR210794 representing BC075709
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGTGGTTGGGAGACTCCAAGAACATGGTGGTGAATGGCAGGAGAAATGGAGGCAAGTTGTCTAATG ACCATCAGCAGAATCAATCAAAATTACAGCAGCACTCGGGCAAGGACACCCTGAAGACCGGCAGAAACGC CGTTGAGAGGCGGTCCAACAGATGTCATGGTAACTCGGGATTTGAAGGGCAGAGCCGCTATGTGCCGTCC TCTGGAATGTCCGCCAAGGAGCTCTGTGAGAACGATGACTTAGCAACCAGTTTGGTTCTTGATCCCTACT TAGGTTTTCAGACACACAAAATGAACACTAGATTTCGGCCTATAAAAGGAAGGCAAGAAGAGCTAAAGGA AGTAATTGAACGCTTTAAGAAAGATGAACACTTAGAGAAAGCTTTCAAATGTTTGACTTCTGGGGAATGG GCACGGCATTATTTTCTCAACAAAAACAAAATGCAGGAGAAATTATTCAAGGAACATGTCTTTATTTACT TGCGGATGTTTGCAACTGACAGTGGATTTGAAATACTGCCTTGTAATAGATATTCTTCAGAACAAAATGG AGCCAAGATAGTTGCAACAAAAGAGTGGAAACGAAATGACAAAATAGAATTACTGGTGGGTTGTATTGCC GAACTTTCAGAAATTGAGGAGAACATGCTACTTAGACACGGAGAAAACGACTTCAGTGTCATGTATTCCA CAAGGAAAAATTGTGCTCAACTCTGGCTCGGTCCTGCTGCATTTATAAATCATGATTGCAGACCTAACTG TAAGTTTGTGTCAACTGGTCGAGATACAGCATGTGTTAAGGCTCTGAGAGATATTGAACCTGGAGAAGAA ATTTCTTGTTACTATGGAGATGGCTTTTTTGGAGAAAATAATGAGTTCTGCGAATGTTATACTTGTGAAA GACGGGGAACTGGTGCTTTTAAATCACGAGTAGGACTGCCTGCGCCTGCTCCTGTTATCAATAGCAAATA CGGACTTAGAGAAACAGATAAACGCTTAAATAGGCTTAAAAAGTTAGGTGACAGCAGCAAAAACTCAGAC AGTCAGTCTGTCAGCTCTAACACAGATGCAGACACCACTCAGGAAAAAGACAATGCAACTTCTAATCGAA AATCTTCAGTTGGTGTGAAAAAGAGCAGCAAGAGTCGAGCTCTGACGAGGCCGTCCATGCCGAGAGTCCC GGCTGCTTCCAACTCTACCTCAGCCAAGCTAGTGCACACCAACAATCCCCGGGTACCAAAGAAACTGAGA AAGCCGGCAAAGCCTTTACTCTCCAAGATCAGACTGCGGAATCACTGCAAGCGGCTGGACCAGAAGAGCG CATCCCGCAAGCTCGAGATGGGGAGCTTAGTGCTTAAGGAGCCCAAAGTTGTGCTATATAAAAATTTGCC AATTAAGAAAGAAAGGGAGCCAGAGGGACCAGCCCATGCTGCAGTGGGGAGTGGGTGCTTGACTAGACAT GCTGCGAGAGAACACAGGCAGAGTCATGGGAGAGGTGCTCATTCGCAGGGCGACAGTTTGCCCTGCACCT ACACAACCCGGCGCTCTTTGAGGACAAGGACAGGTCTGAAGGAGACCACTGACATCAAGCTTGAACCAAG TCCCTTGGATGGCTATAAAAATGGTATACTGGAACCTTGCCCAGACAGTGGCCAGCAGCCAACCCCAGAG GTGCTGGAAGAACTGGCTCCTGAGACTGCACACAGGGAGGAAGCATCCCAGGAGTGTCCCAAGAACGACT CCTGCCTGTCACGAAAGAAATTTCGACAAGTGAAACCTGTGAAACACTTAGCAAAGACAGAGGACTGCAG TCCAGAGCACAGCTTCCCTGGGAAAGACGGGCTGCCAGATTTGCCAGGGTCTCATCCTGATCAAGGTGAG CCCAGTGGCACAGTCAGGGTGCCCGTGAGCTACACGGACTCTGCTCCCTCACCGGTTGGCTGCTCTGTTG TCACACCCGACAGCTTCACAAAAGACAGCTTCAGAACTGCACAAAGTAAAAAGAAGCGGCGGGTCACCAG GTACGATGCACAGCTGATCCTGGAGAACAGCTCTGGAATCCCCAAGCTGACGCTTCGCAGGCGGCACGAC AGCAGCAGCAAGACAAACGACCATGAGAGTGACGGCGTGAACTCCTCCAAGATCAGCATCAAACTCAGCA AGGACCACGACAGTGACAGCAACCTCTATGTTGCCAAGCTCAGTAACGGGGTCAGCGCGGGGCCGGGCAG CAGCTCCACCAAGCTCAAGATCCAGCTCAAGCGGGATGAGGAGAGCAGGGGGCCATGTGCAGAGGGCCTG CACGAGAACGGGGTGTGCTGCAGCGACCCCCTCTCCCTGCTCGAGTCCCAGATGGAGGTGGACGACTACA GTCAATATGAGGAGGACAGCACAGATGAATCCTCATCTTCCTCCGGCAAAGCGGCTGAGGCTAATTGTTG G ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR210794 representing BC075709
Red=Cloning site Green=Tags(s) MKWLGDSKNMVVNGRRNGGKLSNDHQQNQSKLQQHSGKDTLKTGRNAVERRSNRCHGNSGFEGQSRYVPS SGMSAKELCENDDLATSLVLDPYLGFQTHKMNTRFRPIKGRQEELKEVIERFKKDEHLEKAFKCLTSGEW ARHYFLNKNKMQEKLFKEHVFIYLRMFATDSGFEILPCNRYSSEQNGAKIVATKEWKRNDKIELLVGCIA ELSEIEENMLLRHGENDFSVMYSTRKNCAQLWLGPAAFINHDCRPNCKFVSTGRDTACVKALRDIEPGEE ISCYYGDGFFGENNEFCECYTCERRGTGAFKSRVGLPAPAPVINSKYGLRETDKRLNRLKKLGDSSKNSD SQSVSSNTDADTTQEKDNATSNRKSSVGVKKSSKSRALTRPSMPRVPAASNSTSAKLVHTNNPRVPKKLR KPAKPLLSKIRLRNHCKRLDQKSASRKLEMGSLVLKEPKVVLYKNLPIKKEREPEGPAHAAVGSGCLTRH AAREHRQSHGRGAHSQGDSLPCTYTTRRSLRTRTGLKETTDIKLEPSPLDGYKNGILEPCPDSGQQPTPE VLEELAPETAHREEASQECPKNDSCLSRKKFRQVKPVKHLAKTEDCSPEHSFPGKDGLPDLPGSHPDQGE PSGTVRVPVSYTDSAPSPVGCSVVTPDSFTKDSFRTAQSKKKRRVTRYDAQLILENSSGIPKLTLRRRHD SSSKTNDHESDGVNSSKISIKLSKDHDSDSNLYVAKLSNGVSAGPGSSSTKLKIQLKRDEESRGPCAEGL HENGVCCSDPLSLLESQMEVDDYSQYEEDSTDESSSSSGKAAEANCW TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC075709 |
| ORF Size | 2451 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC075709.1 |
| RefSeq Size | 3901 bp |
| RefSeq ORF | 2453 bp |
| Locus ID | 225888 |
| MW | 143 kDa |
| Gene Summary | Histone methyltransferase that specifically trimethylates 'Lys-20' of histone H4. H4 'Lys-20' trimethylation represents a specific tag for epigenetic transcriptional repression. Mainly functions in pericentric heterochromatin regions, thereby playing a central role in the establishment of constitutive heterochromatin in these regions. KMT5B is targeted to histone H3 via its interaction with RB1 family proteins (RB1, RBL1 and RBL2). Plays a role in myogenesis by regulating the expression of target genes, such as EID3.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC220904 | Suv420h1 (untagged) - Mouse suppressor of variegation 4-20 homolog 1 (Drosophila) (cDNA clone MGC:78059 IMAGE:3661032), (10ug) |
CNY 9,120.00 |
|
| MG210794 | Suv420h1 (tGFP-tagged) - Mouse suppressor of variegation 4-20 homolog 1 (Drosophila) (cDNA clone MGC:78059 IMAGE:3661032) |
CNY 9,030.00 |
|
| MR210794L3 | Lenti ORF clone of Suv420h1 (Myc-DDK-tagged) - Mouse suppressor of variegation 4-20 homolog 1 (Drosophila) (cDNA clone MGC:78059 IMAGE:3661032) |
CNY 10,070.00 |
|
| MR210794L4 | Lenti ORF clone of Suv420h1 (mGFP-tagged) - Mouse suppressor of variegation 4-20 homolog 1 (Drosophila) (cDNA clone MGC:78059 IMAGE:3661032) |
CNY 10,070.00 |
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