Kif18b (NM_197959) Mouse Tagged ORF Clone
CAT#: MR210881
- TrueORF®
Kif18b (Myc-DDK-tagged) - Mouse kinesin family member 18B (Kif18b)
ORF Plasmid: tGFP
"NM_197959" in other vectors (3)
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CNY 6,112.00
CNY 7,130.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | 3000004C01Rik |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR210881 representing NM_197959
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTGATGGCTGTGGAAGACAGCGTGGTTCGGGTGGTCGTGCGGGTGAGGCCTCCCACCCCTAAGGAGC TAGAGAGTCAGCGGCGGCCTGTGATTCAGGTGGTAGACGAGCGGATGCTGGTATTTGACCCCGAGGAGTG TGATGGGGGATTTCCTGGCCTAAAATGGAGTGGTTCCCACAATGGCCCCAAGAAGAAGGGCAAAGACCTG ACATTTGTCTTTGACCGTGTCTTTGGTGAGATGGCCACTCAGGAGGATGTGTTCCAGCACACCACACACA ACATCCTCGACAGCTTTCTCCAGGGCTACAACTGCTCAGTGTTTGCCTATGGCGCCACCGGGGCTGGGAA GACACACACTATGCTGGGGAGGGAGGGAGAGCCCGGCATCATGTACCTGACCACCATGGAACTGTACAGG CGTCTGGAAGCCCGCCAGGAAGAAAAGCAATTCGAGGTGCTCATCAGTTACCTGGAGGTGTACAATGAGC AGATCCATGACCTCCTGGAGCCCAAAGGACCCCTCACCATCCGTGAGGACCCAGATAAGGGTGTTGTGGT GCCGGGACTTTCTTTCCACCAGCCAGCCTCAGCCGAGCAGTTACTTGAGATGCTGACAAGAGGCAACTGT AGCCGCACACAGCACCCCACGGATGCCAACGCCACATCCTCCCGCTCTCATGCCATTTTCCAGATCTTTG TGAAGCAGCAGGACCGGGTTCCAGGACTGACCCAGGCCCTTCGAGTAGCCAAGATGAGCTTGATTGACCT GGCTGGCTCCGAGCGGGCATCCAGCACCCATGCCAAAGGAGAGCGGCTGCGGGAGGGTGCCAACATCAAC CGCTCTCTGTTAGCACTCATTAATGTCCTCAATGCCCTGGCTGATGCAAAGGGCCGCAAGTCTCACGTAC CCTACCGAGACAGCAAGCTGACCCGCCTGCTCAAGGACTCCATCGGGGGCAACTGCCGCACAGTGATGAT TGCTGCCATCAGCCCTTCCAGCCTGACCTACGAGGATACTTACAACACCCTCAAATATGCTGACCGTGCC AAGGAGATCAGACTCACGCTGAAGAGCAATGTGATCAGCGTGGACCATCACATTAGCCAATATGCCACCA TCTGCCAGCAGCTCCAGGCTGAGGTAGCCTTCCTGAGGGAGAAACTCCAGATGTATGAGGCTGGAGCCCA GGCCCTGCAACAGCAGTGCTCTCCACAGCCTCCCACGTTGAGCATTCCACAAAGCCTTTCTAGCTCCTCG TTACAGCCTGGGCCCTCCAGCCAGTCCTCTACCCTAGAGTGCCATGCAAAGAATGAAACTCTTCAAGAGG AGAGCCTGGGATCAGACGCCCAGGGACAGGAGATTGTGGAAGAAAGTGCTTCAGAGCAGGAGCAGTGTCC CCAGGACAAACAGTGTCCAACCCAGAAGCCAGAGCCGAACCTGCCCGGGTCCCCTAGTCCAAGTGTACAG GCAAAGCCAGGCACGGGCCAACACTCACCACAGAAACAGGATGCGGACCACTCCAAACAGTTGGCCCTCC AGGTGCTCCGCCTGGCCCAGCGACAATACTCCCTGCTGCAGGCAGCCAACCTCCTGACTCCTGACATGAT CTCAGAGTTTGAGACCCTGCAGCAACTAGTGCTCGAGGAAAGCGTTGACCACAGGGCTGAGAGCCCCAGG TCTCCTGCTCTGGCCAGAGGGGACCCACTAGCTCAGGCGCTGTGTTCCGAGTCTAAGTCTTCAGGGTACT GTGGTCCAGTGACCAGGACCATGGCAAAGCAACTGAATGGTCTCACACACACTCTGGGGGCCCCACTCGC ACCTGACTGCACCTCAGATAAGACATTCCAAAAGCCTACCAAGGAGAAGAAAAGGAAGCTGACCCCCGAA GAGCCAGGCAGCCTACCAGCCCCGAATCTGGAGATGAAGCGCCAGCGCCAGTCCTTCCTCCCCTGCCTAA GAAGGGGCTCTCTACCTAAGGCCCAGCCGTGCTCTGAGCCGAGAACTCCCAAAAGAGAGAGGGCCTCTTC CCCCTCTCCCTCCTCCCGAGTCTGCCCAGCCACGGTCATTAAAAGCCGGGTACCCCTGGGCCCTTCTGCC TTACAGAACTGTTCCACTCCTCTGGCCCTACCCACTCGGGACCTCAACACCACCTTCAATGTCTCTGAGG AGTCCCCCTCCAAACCCAGCTTCCAGGAATTTGTTGACTGGGAAAAAGTTTCCCCGGAGCTGAACAGTAC CGACCAGCCCTTTCTCCCCAGCGCACCTGTCTTCATATTCACGAAGGGCCGCAAGCCTTCGCTTCCTGCA GTCACTGCCAGCAAGAAGAGGCGTACTATGAGGCCCTCAGTCTCCCGAGGCCGCAGCTGTATTGCCCGTC TCCACAGTAGCACCTTGAAGAAGCCAAACAGACCCTTCACTGTCCCAGAGCCACCCTTGAGCCCCCACTG CCTTGATGACCAGAGGACTCCTAAAGGACTGACTGGGGTGACAGAGTCCTAT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR210881 representing NM_197959
Red=Cloning site Green=Tags(s) MVMAVEDSVVRVVVRVRPPTPKELESQRRPVIQVVDERMLVFDPEECDGGFPGLKWSGSHNGPKKKGKDL TFVFDRVFGEMATQEDVFQHTTHNILDSFLQGYNCSVFAYGATGAGKTHTMLGREGEPGIMYLTTMELYR RLEARQEEKQFEVLISYLEVYNEQIHDLLEPKGPLTIREDPDKGVVVPGLSFHQPASAEQLLEMLTRGNC SRTQHPTDANATSSRSHAIFQIFVKQQDRVPGLTQALRVAKMSLIDLAGSERASSTHAKGERLREGANIN RSLLALINVLNALADAKGRKSHVPYRDSKLTRLLKDSIGGNCRTVMIAAISPSSLTYEDTYNTLKYADRA KEIRLTLKSNVISVDHHISQYATICQQLQAEVAFLREKLQMYEAGAQALQQQCSPQPPTLSIPQSLSSSS LQPGPSSQSSTLECHAKNETLQEESLGSDAQGQEIVEESASEQEQCPQDKQCPTQKPEPNLPGSPSPSVQ AKPGTGQHSPQKQDADHSKQLALQVLRLAQRQYSLLQAANLLTPDMISEFETLQQLVLEESVDHRAESPR SPALARGDPLAQALCSESKSSGYCGPVTRTMAKQLNGLTHTLGAPLAPDCTSDKTFQKPTKEKKRKLTPE EPGSLPAPNLEMKRQRQSFLPCLRRGSLPKAQPCSEPRTPKRERASSPSPSSRVCPATVIKSRVPLGPSA LQNCSTPLALPTRDLNTTFNVSEESPSKPSFQEFVDWEKVSPELNSTDQPFLPSAPVFIFTKGRKPSLPA VTASKKRRTMRPSVSRGRSCIARLHSSTLKKPNRPFTVPEPPLSPHCLDDQRTPKGLTGVTESY TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_197959 |
ORF Size | 2502 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_197959.2, NP_932063.2 |
RefSeq Size | 3345 bp |
RefSeq ORF | 2505 bp |
Locus ID | 70218 |
UniProt ID | Q6PFD6 |
MW | 92.4 kDa |
Gene Summary | In complex with KIF2C, constitutes the major microtubule plus-end depolymerizing activity in mitotic cells. Its major role may be to transport KIF2C and/or MAPRE1 along microtubules (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MG210881 | Kif18b (tGFP-tagged) - Mouse RIKEN cDNA 3000004C01 gene (3000004C01Rik) |
CNY 7,890.00 |
|
MR210881L3 | Lenti ORF clone of Kif18b (Myc-DDK-tagged) - Mouse kinesin family member 18B (Kif18b) |
CNY 9,030.00 |
|
MR210881L4 | Lenti ORF clone of Kif18b (mGFP-tagged) - Mouse kinesin family member 18B (Kif18b) |
CNY 9,030.00 |