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Kdm2a (NM_001001984) Mouse Tagged ORF Clone
CAT＃: MR210889
- TrueORF®
Kdm2a (Myc-DDK-tagged) - Mouse lysine (K)-specific demethylase 2A (Kdm2a)
"NM_001001984" in other vectors (3)
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CNY 6,120.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 5530401A10Rik; 100043628; AA589516; AW536790; Cxxc8; Fbl7; Fbl11; Fbxl11; Gm4560; Jhdm1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR210889 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAACCTGAAGAAGAAAGGATTCGGTACAGCCAGAGATTGCGTGGTACCATGCGTCGTCGCTATGAAG ATGATGGCATTTCAGATGATGAAATTGAAGGGAAAAGAACTTTTGACTTGGAAGAGAAGCTCCAAACCAA CAAATATAATGCCAATTTTGTTACTTTTATGGAGGGAAAAGATTTTAATGTAGAGTATATCCAGCGGGGT GGCTTGAGAGACCCTCTCATTTTCAAGAATTCTGATGGACTTGGAATAAAGATGCCGGATCCAGACTTCA CAGTGAATGATGTCAAAATGTGTGTGGGGAGTCGTCGGATGGTGGATGTCATGGATGTGAACACACAGAA GGGGATTGAAATGACCATGGCACAATGGACACGATACTATGAGACTCCAGAGGAAGAGCGAGAAAAACTC TATAATGTTATCAGCCTAGAGTTTAGCCACACCAGGCTTGAGAATATGGTGCAGCGGCCTTCCACGGTGG ATTTCATTGACTGGGTAGATAACATGTGGCCAAGGCACTTGAAAGAAAGTCAGACAGAATCAACAAATGC CATCTTAGAGATGCAGTACCCTAAAGTGCAAAAGTACTGTCTAATGAGTGTTCGAGGCTGCTATACTGAC TTCCATGTGGATTTTGGAGGTACTTCTGTTTGGTATCACATCCACCAAGGTGGAAAGGTCTTCTGGCTCA TCCCCCCTACAGCCCACAACCTGGAGCTGTACGAGAATTGGCTGCTATCAGGGAAACAGGGAGACATCTT TCTGGGTGACCGGGTGTCAGATTGCCAACGAATTGAGCTCAAGCAGGGCTATACCTTCGTTATTCCCTCA GGTTGGATTCATGCTGTGTATACTCCTACAGACACATTAGTGTTTGGAGGCAATTTTTTGCATAGCTTCA ACATCCCCATGCAATTAAAGATATACAGCATTGAAGATCGAACACGGGTTCCAAATAAATTCCGTTACCC ATTTTACTATGAAATGTGTTGGTATGTGTTGGAGCGCTATGTATACTGCATAACCAACCGATCCCACCTA ACTAAGGATTTTCAGAAAGAATCCCTTAGCATGGATATGGAGTTAAATGAGTTGGAGTCTGGAAATGGTG ATGAGGAAGGGGTGGACAGAGAAGCCCGACGCATGAACAATAAGCGATCTGTGCTTACCAGCCCTGTTGC TAATGGAGTGAACCTGGATTACGATGGACTTGGCAAAGCCTGCCGAAGTCTTCCAAGTCTGAAGAAAACT TTGTCTGGAGACTCATCCTCAGACTCTACCCGGGGATCCCACAATGGCCAAGTTTGGGATCCCCAATGTA GCCCTAAAAAGGATAGGCAAGTGCATCTCACCCATTTTGAACTTGAAGGTCTTCGATGTCTTGTAGATAA GTTAGAGTCACTGCCACTGCACAAGAAGTGTGTCCCCACAGGAATAGAAGACGAAGATGCTCTGATTGCT GATGTAAAGATTTTGCTGGAAGAACTTGCCAGTAGCGATCCCAAGTTAGCCCTCACTGGAGTCCCTATAG TACAGTGGCCAAAAAGGGATAAGCTTAAATTCCCTACCAGGCCAAAGGTGAGGGTTCCTACAATTCCCAT CACAAAGCCTCACACCATGAAGCCAGCTCCACGCTTAACACCTGTAAGGCCTGCTGCAGCCTCCCCCATT GTGTCAGGAGCCAGGCGGAGAAGAGTGCGGTGCAGGAAATGCAAAGCTTGTGTGCAAGGAGAATGTGGAG TCTGCCACTACTGCAGGGACATGAAGAAATTTGGTGGACCTGGACGCATGAAGCAATCCTGTGTCCTCCG ACAGTGCTTAGCACCCAGACTGCCTCATTCAGTTACGTGTTCTCTCTGTGGAGAAGTAGATCAGAATGAA GAGACCCAGGACTTTGAAAAGAAACTCATGGAATGCTGCATCTGCAACGAGATAGTTCATCCTGGCTGCC TCCAGATGGATGGAGAGGGGTTGCTGAACGAGGAATTGCCAAATTGCTGGGAGTGTCCAAAGTGTTACCA GGAAGACAGCTCGGACAAAGCCCAGAAGCGGAAAATAGAAGAGAGTGATGAAGAAGCTGTACAAGCCAAA GTCTTACGGCCCCTGAGGAGCTGCGAGGAGCCTCTCACACCCCCGCCTCACTCACCTACTTCCATGCTGC AGCTCATCCACGACCCGGTTTCTCCCCGGGGTATGGTGACTCGGTCATCCCCTGGGGCTGGCCCCAGCGA CCACCACAGTGCCAGCCGTGATGAACGCTTCAAACGGCGGCAGTTGCTGCGGCTACAAGCCACCGAGCGC ACCATGGTACGGGAAAAGGAGAACAATCCCAGCGGCAAAAAGGAGCTGTCTGAAGTTGAGAAAGCCAAGA TCCGGGGATCGTACCTCACTGTCACTCTACAGAGGCCCACCAAAGAGCTCCACGGGACATCCATTGTGCC CAAGCTGCAGGCCATCACGGCCTCCTCTGCCAACCTTCGCCCTAACCCCCCGTGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR210889 protein sequence
Red=Cloning site Green=Tags(s) MEPEEERIRYSQRLRGTMRRRYEDDGISDDEIEGKRTFDLEEKLQTNKYNANFVTFMEGKDFNVEYIQRG GLRDPLIFKNSDGLGIKMPDPDFTVNDVKMCVGSRRMVDVMDVNTQKGIEMTMAQWTRYYETPEEEREKL YNVISLEFSHTRLENMVQRPSTVDFIDWVDNMWPRHLKESQTESTNAILEMQYPKVQKYCLMSVRGCYTD FHVDFGGTSVWYHIHQGGKVFWLIPPTAHNLELYENWLLSGKQGDIFLGDRVSDCQRIELKQGYTFVIPS GWIHAVYTPTDTLVFGGNFLHSFNIPMQLKIYSIEDRTRVPNKFRYPFYYEMCWYVLERYVYCITNRSHL TKDFQKESLSMDMELNELESGNGDEEGVDREARRMNNKRSVLTSPVANGVNLDYDGLGKACRSLPSLKKT LSGDSSSDSTRGSHNGQVWDPQCSPKKDRQVHLTHFELEGLRCLVDKLESLPLHKKCVPTGIEDEDALIA DVKILLEELASSDPKLALTGVPIVQWPKRDKLKFPTRPKVRVPTIPITKPHTMKPAPRLTPVRPAAASPI VSGARRRRVRCRKCKACVQGECGVCHYCRDMKKFGGPGRMKQSCVLRQCLAPRLPHSVTCSLCGEVDQNE ETQDFEKKLMECCICNEIVHPGCLQMDGEGLLNEELPNCWECPKCYQEDSSDKAQKRKIEESDEEAVQAK VLRPLRSCEEPLTPPPHSPTSMLQLIHDPVSPRGMVTRSSPGAGPSDHHSASRDERFKRRQLLRLQATER TMVREKENNPSGKKELSEVEKAKIRGSYLTVTLQRPTKELHGTSIVPKLQAITASSANLRPNPPC TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001001984 |
| ORF Size | 2508 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001001984.1 |
| RefSeq Size | 7268 bp |
| RefSeq ORF | 3486 bp |
| Locus ID | 225876 |
| UniProt ID | P59997 |
| MW | 95.6 kDa |
| Gene Summary | Histone demethylase that specifically demethylates 'Lys-36' of histone H3, thereby playing a central role in histone code. Preferentially demethylates dimethylated H3 'Lys-36' residue while it has weak or no activity for mono- and tri-methylated H3 'Lys-36'. May also recognize and bind to some phosphorylated proteins and promote their ubiquitination and degradation. Required to maintain the heterochromatic state. Associates with centromeres and represses transcription of small non-coding RNAs that are encoded by the clusters of satellite repeats at the centromere. Required to sustain centromeric integrity and genomic stability, particularly during mitosis (By similarity). Regulates circadian gene expression by repressing the transcriptional activator activity of CLOCK-ARNTL/BMAL1 heterodimer and RORA in a catalytically-independent manner (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC223755 | Kdm2a (untagged) - Mouse lysine (K)-specific demethylase 2A (Kdm2a), (10ug) |
CNY 14,920.00 |
|
| MR210889L3 | Lenti ORF clone of Kdm2a (Myc-DDK-tagged) - Mouse lysine (K)-specific demethylase 2A (Kdm2a) |
CNY 10,260.00 |
|
| MR210889L4 | Lenti ORF clone of Kdm2a (mGFP-tagged) - Mouse lysine (K)-specific demethylase 2A (Kdm2a) |
CNY 10,260.00 |
