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Pygm (NM_011224) Mouse Tagged ORF Clone
CAT＃: MR210923
- TrueORF®
Pygm (Myc-DDK-tagged) - Mouse muscle glycogen phosphorylase (Pygm)
ORF Plasmid: tGFP
"NM_011224" in other vectors (3)
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CNY 6,168.00
CNY 7,410.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | AI115133; P; PG |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR210923 representing NM_011224
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCCAGGCCTCTTTCAGACCAGGATAAGAGAAAGCAAATCAGCGTTCGTGGCTTAGCCGGAGTGGAAA ATGTGTCGGAGCTAAAAAAGAACTTCAACCGCCACCTGCATTTCACCTTGGTCAAGGATCGCAATGTGGC TACTCCGAGAGATTACTATTTTGCACTGGCCCACACTGTCCGGGACCACCTCGTGGGACGCTGGATCCGC ACACAGCAGCATTACTACGAAAAGGACCCCAAGAGGATCTACTACCTGTCTTTGGAGTTCTACATGGGCC GGACACTGCAGAACACCATGGTGAACCTGGCCTTGGAGAACGCCTGTGATGAGGCCACTTACCAGCTGGG CTTGGACATGGAGGAGTTGGAGGAGATCGAGGAGGATGCAGGGTTGGGCAATGGGGGCCTGGGACGCCTG GCAGCTTGCTTTTTGGACTCCATGGCCACCCTGGGGCTCGCTGCCTATGGCTATGGGATCCGCTATGAGT TTGGGATTTTTAATCAGAAGATCTGTGGGGGCTGGCAGATGGAAGAAGCTGATGACTGGCTTCGCTATGG CAACCCCTGGGAGAAGGCCCGCCCAGAGTTCACGCTGCCTGTGCACTTCTATGGCCGAGTGGAGCACACC AGCCAGGGTGCCAAGTGGGTGGACACACAGGTAGTGCTGGCTATGCCCTATGACACTCCTGTGCCCGGCT ATCGGAACAACGTCGTCAACACCATGCGCCTCTGGTCGGCCAAGGCACCTAACGACTTCAACCTCAAGGA CTTCAATGTTGGCGGCTACATCCAGGCTGTGCTGGACCGAAACCTGGCCGAGAACATCTCGCGTGTTTTG TACCCCAATGATAATTTCTTTGAAGGGAAGGAGCTGCGGCTGAAGCAGGAGTACTTTGTGGTGGCTGCCA CCCTCCAGGACATCATCCGACGTTTCAAGTCCTCCAAGTTTGGCAGCCGTGATCCTGTGCGCACAAACTT CGATGCCTTCCCTGATAAGGTGGCCATCCAGCTCAATGACACCCACCCCTCTTTGGCCATCCCAGAGCTG ATGAGGATTCTGGTGGACCTGGAACGACTGGACTGGGACAAGGCCTGGGACGTAACAGTGAAGACCTGTG CCTACACCAACCACACGGTGCTGCCTGAAGCCCTGGAGCGCTGGCCAGTGCACCTCATGGAGACACTGCT GCCCCGCCACTTGCAGATCATTTACGAGATCAACCAGCGTTTCCTTAACCGGGTGGCGGCTGCATTCCCT GGGGACGTAGATAGGCTGCGGCGCATGTCACTCGTGGAGGAAGGAGCAGTGAAACGCATCAACATGGCAC ACCTGTGCATTGCTGGCTCGCACGCTGTCAACGGCGTGGCTCGCATCCATTCGGAGATCCTCAAGAAAAC CATCTTCAAGGACTTTTATGAGCTGGAGCCTCACAAGTTCCAGAACAAGACCAATGGCATCACTCCTCGG CGCTGGCTGGTTCTGTGTAACCCTGGGCTGGCAGAGGTCATTGCTGAGCGCATTGGTGAGGACTACATCT CAGACCTGGACCAGCTGCGCAAGCTGCTCTCCTATGTGGATGATGAAGCCTTTATCCGTGATGTGGCCAA AGTGAAACAGGAAAACAAGTTGAAGTTCTCTGCATACCTCGAGAGGGAATACAAAGTCCACATCAACCCC AACTCGCTCTTTGACGTCCAGGTGAAAAGGATTCATGAGTACAAGCGGCAGCTCCTCAACTGCCTGCACA TCATCACCTTGTATAACCGCATCAAAAGGGAGCCCAATAGGTTTATGGTGCCGAGGACTATCATGATTGG AGGCAAGGCTGCACCCGGGTACCACATGGCTAAGATGATCATCAAGCTTATCACTGCCATTGGGGATGTG GTCAACCACGACCCTGCGGTAGGAGACCGCCTCCGAGTGATCTTCCTGGAGAACTACCGAGTCTCCCTTG CTGAGAAAGTTATTCCCGCCGCCGACCTCTCAGAGCAGATCTCCACCGCGGGCACCGAGGCCTCGGGCAC TGGCAACATGAAGTTCATGCTCAATGGGGCTCTGACCATCGGCACCATGGACGGTGCCAACGTGGAGATG GCAGAGGAGGCGGGGGAGGAGAACTTCTTCATCTTCGGCATGCGAGTGGAGGACGTGGAAAGGCTGGACC AGAGAGGGTACAATGCCCAGGAGTACTACGACCGAATTCCTGAGCTTCGGCAGATCATCGAACAGCTAAG CAGCGGCTTCTTCTCCCCCAAGCAGCCTGACCTGTTCAAGGACATTGTCAATATGCTCATGCACCATGAC CGGTTTAAAGTCTTTGCAGATTATGAGGAATACATTAAATGCCAGGACAAAGTCAGTGAGTTGTACAAGA ACCCAAGAGAGTGGACACGGATGGTGATCAGGAACATAGCCACCTCTGGCAAGTTTTCCAGTGACCGCAC CATTGCCCAGTATGCCCGGGAGATCTGGGGTGTTGAACCTTCTCGCCAGCGCCTGCCAGCCCCAGATGAG AAGATC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR210923 representing NM_011224
Red=Cloning site Green=Tags(s) MSRPLSDQDKRKQISVRGLAGVENVSELKKNFNRHLHFTLVKDRNVATPRDYYFALAHTVRDHLVGRWIR TQQHYYEKDPKRIYYLSLEFYMGRTLQNTMVNLALENACDEATYQLGLDMEELEEIEEDAGLGNGGLGRL AACFLDSMATLGLAAYGYGIRYEFGIFNQKICGGWQMEEADDWLRYGNPWEKARPEFTLPVHFYGRVEHT SQGAKWVDTQVVLAMPYDTPVPGYRNNVVNTMRLWSAKAPNDFNLKDFNVGGYIQAVLDRNLAENISRVL YPNDNFFEGKELRLKQEYFVVAATLQDIIRRFKSSKFGSRDPVRTNFDAFPDKVAIQLNDTHPSLAIPEL MRILVDLERLDWDKAWDVTVKTCAYTNHTVLPEALERWPVHLMETLLPRHLQIIYEINQRFLNRVAAAFP GDVDRLRRMSLVEEGAVKRINMAHLCIAGSHAVNGVARIHSEILKKTIFKDFYELEPHKFQNKTNGITPR RWLVLCNPGLAEVIAERIGEDYISDLDQLRKLLSYVDDEAFIRDVAKVKQENKLKFSAYLEREYKVHINP NSLFDVQVKRIHEYKRQLLNCLHIITLYNRIKREPNRFMVPRTIMIGGKAAPGYHMAKMIIKLITAIGDV VNHDPAVGDRLRVIFLENYRVSLAEKVIPAADLSEQISTAGTEASGTGNMKFMLNGALTIGTMDGANVEM AEEAGEENFFIFGMRVEDVERLDQRGYNAQEYYDRIPELRQIIEQLSSGFFSPKQPDLFKDIVNMLMHHD RFKVFADYEEYIKCQDKVSELYKNPREWTRMVIRNIATSGKFSSDRTIAQYAREIWGVEPSRQRLPAPDE KI TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_011224 |
| ORF Size | 2526 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_011224.2 |
| RefSeq Size | 2874 bp |
| RefSeq ORF | 2529 bp |
| Locus ID | 19309 |
| UniProt ID | Q9WUB3 |
| MW | 97.7 kDa |
| Gene Summary | This gene encodes a glycolysis enzyme found in muscle. Highly similar enzymes encoded by different genes are found in liver and brain. The encoded protein is involved in regulating the breakdown of glycogen to glucose-1-phosphate, which is necessary for ATP generation. [provided by RefSeq, Dec 2015] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG210923 | Pygm (tGFP-tagged) - Mouse muscle glycogen phosphorylase (Pygm) |
CNY 8,170.00 |
|
| MR210923L3 | Lenti ORF clone of Pygm (Myc-DDK-tagged) - Mouse muscle glycogen phosphorylase (Pygm) |
CNY 9,310.00 |
|
| MR210923L4 | Lenti ORF clone of Pygm (mGFP-tagged) - Mouse muscle glycogen phosphorylase (Pygm) |
CNY 9,310.00 |
