Pcdha1 (BC082326) Mouse Tagged ORF Clone

CAT#: MR211110

  • TrueORF®

Pcdha1 (Myc-DDK-tagged) - Mouse protocadherin alpha 1 (cDNA clone MGC:90628 IMAGE:30356350)

ORF Plasmid: DDK tGFP



  "BC082326" in other vectors (6)

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Mouse Protein / Lysate Request

CNY 9,904.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (3)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Synonyms CNR
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR211110 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCTGTTTTCCAGGCTCCGAGGCCTGAGAGCCCGGCGCCTGCTCTTGTTCTCGCTTCTGTTTCTTGCAG
CCTGGGAGGCAGGGAGCGGCCAGATTCATTACTCTGTCCCAGAGGAAGCCAAACACGGCACCTTTGTGGG
CCGCATCGCGCAGGACCTGGGGCTGGAGCTGACGGAGCTGGTGCCCCGCCTATTCAGGGTGGCGTCCAAG
GATCGCGGGGACCTTCTGGAGGTAAATCTGCAGAATGGCATTTTGTTTGTGAATTCTCGGATCGACCGGG
AGGAGCTGTGCGGGCGGAGCGCGGAGTGTAGCATCCACCTGGAGGTGATCGTGGACAGGCCGCTGCAGGT
TTTCCACGTGGAGGTGAAGGTTAAAGATATTAATGATAACCCACCAGTTTTCAAAGGCGCAGAGCAAAGG
ATATTTATTCCTGAAAACAGACAACTAGATTCACGGTTTCCACTAGAGGGCGCCGTGGACGCAGACATTG
GTGCCAATTCTCTTTTAACTTACACGCTGAGCCCCACTGATTACTTTTCTTTAAAGGTAGAGACAACAGA
CGAACTCAGTAAATCCCTTTCGCTTGAACTGAGAAAATCTTTGGATAGAGAAGAAACACCAGAACTTCAG
TTATTACTGACAGCCACCGACGGGGGCAAGCCAGAGCTGGAGGGGACAGTTCGGCTGCAGATTACTGTGC
TCGATGTTAATGACAACGCCCCACTGTTTGACCAGGCCATCTACAGAGCACAGTTAGTAGAATCTACAGT
GAATGGGACATTAGTGACGACGTTAAATGCCACTGACGCTGATGAAGGTGTTAATGGTGAAGTTGTGTTT
TCCTTTGGCAATGACGTTTCTCTTGACATTCAAGAAAAATTCAATGTGGATTCTCTTTCAGGAGAAATTA
GAGTGATCGGTGATCTAGATTATGAAAAAACAAAATCCTATGAAATTCAAATAAAGGCAGTTGATAAAGG
GACTCCCTCCATGTCAAATCACTGCAAGGTTTTGGTGAAAGTACTGGATATAAATGATAATGCTCCAGAG
CTGTCGATCACATCCTTGTCTTTGCCCATCAAAGAGGATACTCCACTTAACACTATCATCGCACTCATCA
AGGTGTCCGACATCGACTCAGGTGTCAACGGGCAGGTGACCTGCTCCCTAACTCCTCATGTCCCTTTCAA
GTTGGTGTCCACCTTCAAGAATTACTATTCGCTTGTGCTGGACAGCACCCTGGACCGAGAGACCACAGCG
GACTATAAGGTGGTGGTGACAGCCCGCGACGGGGGCTCGCCCTCGCTGTGGGCCACGGCCAGCGTGTCTG
TGGAGGTGGCTGACGTGAACGACAATGCTCCAGTGTTCGCGCAGCCCGAATACACTGTGTTCGTGAAGGA
GAACAACCCGCCTGGTGCGCACATCTTCACGGTGTCAGCGATGGACGCGGATGCGCAGGAGAATGCGCTG
GTGTCCTACTCGCTGGTGGAGCGGAGGGTGGGCGAGCGCTTGCTGTCGAGCTATGTGTCTGTGCACGCGG
AGAGCGGCAAGGTGTTCGCGCTGCAGCCTCTGGACCATGAGGAGCTGGAGCTGCTGCAGTTCCAGGTGAG
CGCGCGGGATGCTGGTGTGCCTGCCCTGGGCAGCAATGTGACTCTGCAGGTGTTTGTGCTGGACGAGAAT
GACAATCCACCCACACTGCTGGGGCCTCACGCAGGCAGCGCAGTGAGCGAACTGGTGTCACGGACAATGG
GTGCAGGGCATGTGGTGACAAAGGTGCGCGCGGTGGACGCGGACTCTGGCTACAATGCGTGGCTGTCCTA
TGAACTACAACCTCCTACAGGTGGTGCGCGCAACCCGTTTCGCGTGGGTCTGTACACTGGTGAGATTAGC
ACCACACGCTCCTTAGATGAGGTCGATGTTCCACGACAGCGTCTCTTGGTGCTGGTGAAGGACCATGGAG
AGCCAATGCTGACTGCTACCGCTACCGTGTTGGTATCTCTGGTGGAAAGCAGTCCGATTCAGAAAGCCTC
TTTGCCTGTATTAACCGGTGCAGTGGGTTCCAAGGCATCGCTGGTGGATGTCAACGTGTACCTGATCATC
GCCATCTGTGCCGTGTCCAGCCTGTTAGTGCTCACTTTGCTTCTGTACACAGCGCTGCGCTGCTCCGCGA
CACCCACTGATGGAGCCTGTGGTCCGGGGAAGCCCATGCTGGTGTGCTCCAGCGCGGTGGGGAGCTGGTC
ATACTCGCAGCAGAGGCGACAGAAGGTGTGCTCTGGAGAGGGTCCACCTAAGACCGACCTCATGGCCTTC
AGCCCGGGTTTAGCCCCAGGTTTGAACATAGCGGAAAGAAGTGACCACGCAGAAGTAAATTCAGATCTTT
CTGGTAATCCCCGGCAGCCCAACCCTGACTGGCGCTACTCTGCCTCGCTAAGAGCAGGCATGCACAGCTC
TGTGCACCTGGAGGAGGCTGGCATTCTACGGGCTGGTCCAGGAGGGCCTGATCAGCAGTGGCCAACAGTA
TCCAGTGCAACACCAGAACCTGAGGCAGGAGAGGTGTCCCCTCCGGTGGGCGCCGGTGTCAACAGCAACA
GCTGGACCTTTAAATACGGACCAGGCAACCCCAAACAGTCCGGTCCCGAACCTAAAAAGCAGACCCAGGT
TTCCTTTCTCCCCCGCCGCAAAGGAGAAGCTTCCCAGCCCCGCCAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR211110 protein sequence
Red=Cloning site Green=Tags(s)

MLFSRLRGLRARRLLLFSLLFLAAWEAGSGQIHYSVPEEAKHGTFVGRIAQDLGLELTELVPRLFRVASK
DRGDLLEVNLQNGILFVNSRIDREELCGRSAECSIHLEVIVDRPLQVFHVEVKVKDINDNPPVFKGAEQR
IFIPENRQLDSRFPLEGAVDADIGANSLLTYTLSPTDYFSLKVETTDELSKSLSLELRKSLDREETPELQ
LLLTATDGGKPELEGTVRLQITVLDVNDNAPLFDQAIYRAQLVESTVNGTLVTTLNATDADEGVNGEVVF
SFGNDVSLDIQEKFNVDSLSGEIRVIGDLDYEKTKSYEIQIKAVDKGTPSMSNHCKVLVKVLDINDNAPE
LSITSLSLPIKEDTPLNTIIALIKVSDIDSGVNGQVTCSLTPHVPFKLVSTFKNYYSLVLDSTLDRETTA
DYKVVVTARDGGSPSLWATASVSVEVADVNDNAPVFAQPEYTVFVKENNPPGAHIFTVSAMDADAQENAL
VSYSLVERRVGERLLSSYVSVHAESGKVFALQPLDHEELELLQFQVSARDAGVPALGSNVTLQVFVLDEN
DNPPTLLGPHAGSAVSELVSRTMGAGHVVTKVRAVDADSGYNAWLSYELQPPTGGARNPFRVGLYTGEIS
TTRSLDEVDVPRQRLLVLVKDHGEPMLTATATVLVSLVESSPIQKASLPVLTGAVGSKASLVDVNVYLII
AICAVSSLLVLTLLLYTALRCSATPTDGACGPGKPMLVCSSAVGSWSYSQQRRQKVCSGEGPPKTDLMAF
SPGLAPGLNIAERSDHAEVNSDLSGNPRQPNPDWRYSASLRAGMHSSVHLEEAGILRAGPGGPDQQWPTV
SSATPEPEAGEVSPPVGAGVNSNSWTFKYGPGNPKQSGPEPKKQTQVSFLPRRKGEASQPRQ

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC082326
ORF Size 2706 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC082326, AAH82326
RefSeq Size 4942 bp
RefSeq ORF 2708 bp
Locus ID 116731
MW 97.5 kDa
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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