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Dapk1 (BC060161) Mouse Tagged ORF Clone
CAT＃: MR211125
- TrueORF®
Dapk1 (Myc-DDK-tagged) - Mouse death associated protein kinase 1 (cDNA clone MGC:61377 IMAGE:6821014)
ORF Plasmid: tGFP
"BC060161" in other vectors (4)
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CNY 6,632.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | D13Ucla1; DAP-Kinase |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR211125 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACTGTGTTCAGGCAGGAAAACGTGGACGACTACTACGACACCGGCGAGGAACTGGGCAGTGGACAGT TCGCAGTTGTGAAGAAATGTCGTGAGAAAAGTACCGGTCTTCAGTATGCGGCCAAGTTCATCAAGAAAAG GAGGACCAAGTCCAGCCGGCGGGGCGTGAGCCGGGAGGACATCGAGCGGGAGGTCAGCATCCTGAAGGAG ATCCGGCACCCAAATGTCATCACCCTGCATGAGGTCTATGAGAACAAGACAGATGTCATTCTGATCCTGG AGCTTGTTGCAGGAGGTGAGCTGTTTGACTTCCTGGCTGAGAAGGAATCTCTGACTGAAGAGGAGGCAAC GGAATTCCTTAAGCAGATTCTCAGCGGCGTTTACTACCTGCACTCACTGCAGATCGCTCACTTTGACCTG AAGCCGGAAAACATAATGCTTCTGGATAGAAATGTGCCCAAACCTCGGATCAAGATCATAGACTTTGGCT TGGCCCATAAAATTGACTTTGGAAATGAATTCAAAAACATATTTGGGACACCAGAGTTTGTGGCTCCGGA GATAGTCAACTATGAGCCCCTGGGTCTTGAGGCAGATATGTGGAGCATCGGGGTAATAACCTATATCCTC CTAAGTGGGGCCTCCCCTTTTCTTGGAGACACCAAGCAAGAAACATTAGCGAATGTGTCCGCTGTCAACT ACGACTTTGAGGAGGAATTCTTCCGGAACACCAGTACCCTTGCCAAAGATTTCATCAGAAGACTGCTGGT CAAGGATCCAAAGAAGAGGATGACAATCCAGGACAGTTTGCAGCACCCCTGGATCAAGCCTAAAGACACC CAACAAGCACTTAGTCGAAAAGCCTCAGCAGTAAACATGGAGAAATTCAAGAAGTTTGCAGCTCGGAAAA AATGGAAACAATCTGTTCGCTTGATATCACTGTGCCAAAGATTATCCAGGTCATTTTTGTCCAGAAGTAA CATGAGTGTCGCCAGGAGTGATGATACTCTGGATGAGGAAGACTCCTTTGTGATGAAAGCCATCATCCAT GCCATCAATGATGACAACGTACCCGGCCTGCAGCATCTCCTGGGCTCCTTGTCCAGCTATGACGTCAACC AGCCCAACAAGCATGGGACACCTCCATTACTGATTGCCGCAGGCTGTGGCAACATCCAGATGTTACAGTT ACTCATAAAACGAGGCTCAAGGATTGACGTCCAGGATAAGGGAGGATCCAATGCCATCTACTGGGCCTCT CGGCATGGCCATGTGGATACTTTGAAATTTCTCAATGAGAACAAATGCCCTTTGGATGTTCAAGACAAGT CTGGAGAGACAGCTCTTCACGTGGCAGCCCGCTATGGCCATGCAGATGTGGTTCAACTACTGTGCAGTTT TGGCTCTAATCCTGATTTCCAGGACAAGGAAGAGGAAACCCCCCTGCACTGTGCTGCCTGGCATGGCTAT TACTCCGTGGCTAAAGCTCTTTGTGAAGTTGGCTGCAACGTGAATATCAAGAATCGGGAGGGAGAGACCC CATTGCTGACGGCGTCTGCCAGGGGCTATCATGACATTGTGGAGTGTCTGGCTGAACATGGAGCTGACTT GAATGCTTCTGACAAGGATGGACACATCGCTCTTCATCTTGCTGTGAGGCGTTGTCAGATGGAAGTCATC AAGACCCTCCTTGGCCATGGGTCCTTTGTGGATTTCCAGGACAGGCATGGCAACACACCCCTGCACGTGG CCTGCAAAGATGGAAGCGCACCTATCGTGGTGGCCCTCTGTGAAGCCAGCTGCAATCTGGACATCTCAAA CAAGTATGGTCGGACTCCTCTCCACCTTGCAGCCAACAACGGGATCCTAGATGTGGTCCGCTACCTCTGT TTGATGGGCGCCAATGTGGAGGCTCTAACCTCGGATGGAAAGACGGCCGAGGACCTCGCCAAGGCAGAAC AGCACGAGCATGTGGCAGGGCTCCTGGCAAGACTGCGGAAGGACACACACCGAGGACTCTTCATCCAGCA ACTCCGACCCACCCAGAATCTCCAGCCCAGAATCAAGCTCAAACTGTTTGGCCATTCGGGATCAGGGAAA TCCACCCTGGTGGAATCTCTCAAGTGTGGGCTGTTAAGGAGTTTCTTCAGAAGGCGCCGGCCCAGACTAT CCTCTACCAACTCCACCCGCTTCCCACCGTCACCCCTGGCTGCTAAGCCAACAGTCTCAGTGAGCATTAA CAACCTGTACCCCGGCTGTGAGAACGTGAGCGTAAGGAGCCGCAGCATGATGTTCGAGCCGGGCCTCACC AAAGGGATGCTGGAAGTGTTCGTGGCTCCGTCTCACCACCTCCACTGCTCGACTGATGACCAGTCCACCA AAGCCATCGACATCCAGAATGCTTATTTGAACGGAGTTGGTGATTTCAGTGTGTGGGAGTTCTCTGGAAA CCCTGTGTACTTCTGTTGCTATGACTACTTTGCTGCCAACGACCCCACGTCCATCCACATCATCGTTTTC AGTCTCGAAGAACCCTATGAGATCCAGCTGAACCAAGTGATTTTCTGGCTCAGTTTCCTGAAGTCTCTGG TCCCAGTTGAAGAACCCATAGCATTTGGAGGCAAGCTGAAGAACCCTCTCCGAGTTGTCCTGGTGGCAAC ACATGCTGACATCATGAACATCCCTCGGCCTGCTGGAGGCGAGTTTGGATATGAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR211125 protein sequence
Red=Cloning site Green=Tags(s) MTVFRQENVDDYYDTGEELGSGQFAVVKKCREKSTGLQYAAKFIKKRRTKSSRRGVSREDIEREVSILKE IRHPNVITLHEVYENKTDVILILELVAGGELFDFLAEKESLTEEEATEFLKQILSGVYYLHSLQIAHFDL KPENIMLLDRNVPKPRIKIIDFGLAHKIDFGNEFKNIFGTPEFVAPEIVNYEPLGLEADMWSIGVITYIL LSGASPFLGDTKQETLANVSAVNYDFEEEFFRNTSTLAKDFIRRLLVKDPKKRMTIQDSLQHPWIKPKDT QQALSRKASAVNMEKFKKFAARKKWKQSVRLISLCQRLSRSFLSRSNMSVARSDDTLDEEDSFVMKAIIH AINDDNVPGLQHLLGSLSSYDVNQPNKHGTPPLLIAAGCGNIQMLQLLIKRGSRIDVQDKGGSNAIYWAS RHGHVDTLKFLNENKCPLDVQDKSGETALHVAARYGHADVVQLLCSFGSNPDFQDKEEETPLHCAAWHGY YSVAKALCEVGCNVNIKNREGETPLLTASARGYHDIVECLAEHGADLNASDKDGHIALHLAVRRCQMEVI KTLLGHGSFVDFQDRHGNTPLHVACKDGSAPIVVALCEASCNLDISNKYGRTPLHLAANNGILDVVRYLC LMGANVEALTSDGKTAEDLAKAEQHEHVAGLLARLRKDTHRGLFIQQLRPTQNLQPRIKLKLFGHSGSGK STLVESLKCGLLRSFFRRRRPRLSSTNSTRFPPSPLAAKPTVSVSINNLYPGCENVSVRSRSMMFEPGLT KGMLEVFVAPSHHLHCSTDDQSTKAIDIQNAYLNGVGDFSVWEFSGNPVYFCCYDYFAANDPTSIHIIVF SLEEPYEIQLNQVIFWLSFLKSLVPVEEPIAFGGKLKNPLRVVLVATHADIMNIPRPAGGEFGYD TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC060161 |
| ORF Size | 2715 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC060161, AAH60161 |
| RefSeq Size | 5056 bp |
| RefSeq ORF | 2717 bp |
| Locus ID | 69635 |
| MW | 101.2 kDa |
| Gene Summary | Calcium/calmodulin-dependent serine/threonine kinase involved in multiple cellular signaling pathways that trigger cell survival, apoptosis, and autophagy. Regulates both type I apoptotic and type II autophagic cell deaths signal, depending on the cellular setting. The former is caspase-dependent, while the latter is caspase-independent and is characterized by the accumulation of autophagic vesicles. Phosphorylates PIN1 resulting in inhibition of its catalytic activity, nuclear localization, and cellular function. Phosphorylates TPM1, enhancing stress fiber formation in endothelial cells. Phosphorylates STX1A and significantly decreases its binding to STXBP1. Phosphorylates PRKD1 and regulates JNK signaling by binding and activating PRKD1 under oxidative stress. Phosphorylates BECN1, reducing its interaction with BCL2 and BCL2L1 and promoting the induction of autophagy. Phosphorylates TSC2, disrupting the TSC1-TSC2 complex and stimulating mTORC1 activity in a growth factor-dependent pathway. Phosphorylates RPS6, MYL9 and DAPK3 (By similarity). Acts as a signaling amplifier of NMDA receptors at extrasynaptic sites for mediating brain damage in stroke. Cerebral ischemia recruits DAPK1 into the NMDA receptor complex and it phosphorylates GRINB at Ser-1303 inducing injurious Ca(2+) influx through NMDA receptor channels, resulting in an irreversible neuronal death. Required together with DAPK3 for phosphorylation of RPL13A upon interferon-gamma activation which is causing RPL13A involvement in transcript-selective translation inhibition.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC220657 | Dapk1 (untagged) - Mouse death associated protein kinase 1 (cDNA clone MGC:61377 IMAGE:6821014), (10ug) |
CNY 9,120.00 |
|
| MG211125 | Dapk1 (tGFP-tagged) - Mouse death associated protein kinase 1 (cDNA clone MGC:61377 IMAGE:6821014) |
CNY 5,610.00 |
|
| MR211125L3 | Lenti ORF clone of Dapk1 (Myc-DDK-tagged) - Mouse death associated protein kinase 1 (cDNA clone MGC:61377 IMAGE:6821014) |
CNY 7,030.00 |
|
| MR211125L4 | Lenti ORF clone of Dapk1 (mGFP-tagged) - Mouse death associated protein kinase 1 (cDNA clone MGC:61377 IMAGE:6821014) |
CNY 7,030.00 |
