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Itih1 (BC028814) Mouse Tagged ORF Clone
CAT＃: MR211152
- TrueORF®
Itih1 (Myc-DDK-tagged) - Mouse inter-alpha trypsin inhibitor, heavy chain 1 (cDNA clone MGC:36127 IMAGE:5099284)
ORF Plasmid: tGFP
"BC028814" in other vectors (4)
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CNY 9,120.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Intin1; ITI-HC1; Itih-1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR211152 representing BC028814
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGGTGCCACAGGGCTCCGGGTGCTGCTGTGCTTGCTGCCGCTCCTCACTCTGCAGGCCAGGCCTG CTTTGGGCTTGGCAACAGGCAGACCCAGGGGCAGTGAGAAGCGACACGCTGTGGACACTTATTATCTTCA GTCAGTCAATGGTGTGTCCATCAAGAGCTTGAAAGTCAATTGCAAAGTCACCTCTCGCTTTGCCCACTAC GTCATCACCAGCCAAGTGGTCAACAATGCTGATAAAGCCAGGGAAGTGGCTTTTGATGTGGAAATCCCCA AGACAGCCTTCATCAGTGACTTTGCCATAACATCAGATGGGAAGGCGTTCATTGGGGACATAAAGGACAA AGTGACTGCATGGAAGCAGTACCGGAAGGCAGCCGTTTTAGGGGAGAGCGCTGGCCTTGTCAGAGCCTCG GGCAGAAATATGGAGCAGTTCACCATCCATATCACTGTTGGAGCCCAGAGCAAGGCCACGTTCCGGCTCA CCTATGAGGAGGTACTAAAGAGGAGACTGATGCAGTATGACATCACCATCAAAGTCAGACCCAAGCAGCT GGTACAGCATTTTGAGATCGATGTGGACATCTTTGAACCCCAGGGGATCAGCAAGCTGGACGCTCAGGCC TCCTTCCTCAGTGAGGAACTGGCTGCTCAAACCATCAAGAAGTCTTTCTCAGGGAAAAAGGGTCATGTGC TCTTCCGCCCCACTGTGAGCCAGCAGCAGTCCTGCCCCACATGTTCTACATCCTTGCTAAATGGAGAATT CAAGGTGACCTACGATGTCAATCGAGACAAGCTCTGTGACCTCCTGGTGGCCAACAACTACTTTACACAT TTCTTTGCCCCCAAAAACCTGACCAACATGAGCAAGAACCTGGTTTTTGTGATTGACATCAGTGGCTCCA TGGAAGGCCAGAAAGTGAGGCAGACAAAGGAGGCACTCCTTAAGATCCTGGAGGACATGAGGCCAGTAGA CAACTTTGACCTGGTCCTCTTTGGCTCTAAAGTGCAATCATGGAAGGGCTCCCTGGTACCTGTGTCTAAT GCCAATCTGCAAGCAGCTCAAGACTTTGTGAGGCGCTTTTCTTTGGCTGGAGCCACAAACCTGAATGGAG GCTTGCTCCGAGGAATCGAGATCTTAAACAAAGCTCAAGGAAGCCACCCAGAACTCAGCAGCCCGGCCTC AATTCTTATCATGTTGACAGACGGAGAGCCCACTGAAGGGGAGACGGACCGTTCCCAGATCCTCAAGAAT GTACGCAACGCTATCCGGGGCAGGTTCCCGCTCTACAACCTCGGCTTTGGCCACGACCTGGACTTCAGCT TCCTGGAGGTCATGTCCACTGAGAACAACGGATGGGCCCAGAGAATTTATGAGGACCATGATGCCACCCA ACAGCTACAGGGTTTCTACAATCAAGTAGCCAATCCCCTGCTGACAGATGTGGAACTGCAGTACCCCCAG GATGCTGTCTTGGCTCTAACCCAGCACCGACATAAACAGTACTATGACGGCTCGGAGATTGTGGTGGCTG GGCGCATTGCTAACCACAAACTGAACACCTTTAAGGCTGACGTTCGGGCTCGTGGGGAAAAGCAAGAATT CAGGGCAACCTGCCTAGTGGACGAGGAAGAGATGAAGAAACTGCTCCGAGAGCGTGGCCACGTGCTAGAG AATCATGTGGAGAGGCTCTGGGCCTACCTCACGATCCAGGAGCTCCTGGCTAAGCGGATGAAGACGGAAG GGGAGGAGAGGGCCAACCTGTCATCCCAGGTCCTGAAGATGTCACTGGACTATCATTTCGTAACGCCACT GACCTCTCTGACTATCAGAGGCCTGACAGACGAGGATGGGCTGGAGCCCACCATTGACAAGCCCCTGGAG GATTCTCAGCCCTTAGAGATGGTGGGACCCAGAAGAACGTTTGTGCTGTCAGCCATACAGCCATCTCCTA CAGCCCACCCCATAGACTCAAAGTTGCCACTCCGTGTGACAGGTGTGGACACCGATCCCCACTTCATCAT CTATGTGCCCTCAAAAGAGGATAGCTTGTGCTTCAACATCAATGAGGAACCTGGTGTGATCCTGAACCTA GTACAGGACCCTGACACAGGCTTCACCGTGAATGGGCAGCTCATTGGGAACAAGGCCAGCAGCCCTGGGC AACACGAGAGCACATACTTCGGGAGATTGGGAATCTCAAGTCCCACATCAGACTTTCAGCTGGAAGTGAC TCCTCAGAACATTACACTGAACCCCAGCTCCAGCGGGTCCATGTTCTCCTGGAGGGACCAGGCTGTGCTG CAGAAGGATGGGGTAGTGGTGACAATCAACAAGAAGAGGAACCTGGTGGTGTCTGTGGATGATGGGGCTA CCTTTGAGATTGTCCTGCACAGAACATGGAAGGGGAGTGCAGTCCACCAGGACTTCCTGGGTTTCTATGT GCTGGACAGCTTCCGGATGTCAGCCCGGACAAAGGGGCTACTGGGACAATTCTTCTCCCCACTGGATTTT GAAGTATTTGACCTCCACCCAGGCTCTGACCCTACAAAGACAGATGCCACAATGGTGGTGAAGAATCGGC AGCTGACAGTCACCCGAGGGTTGCAAAAAGACTACAGCAAGGACCCCAGACATGGAGCAGAAGTGCCTTG CTGGTTTGTCCACGACAATGGAGCTGGACTGATTGATGGTGTTCACACTGACTATGTCGTCTCTGACATC TTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR211152 representing BC028814
Red=Cloning site Green=Tags(s) MEGATGLRVLLCLLPLLTLQARPALGLATGRPRGSEKRHAVDTYYLQSVNGVSIKSLKVNCKVTSRFAHY VITSQVVNNADKAREVAFDVEIPKTAFISDFAITSDGKAFIGDIKDKVTAWKQYRKAAVLGESAGLVRAS GRNMEQFTIHITVGAQSKATFRLTYEEVLKRRLMQYDITIKVRPKQLVQHFEIDVDIFEPQGISKLDAQA SFLSEELAAQTIKKSFSGKKGHVLFRPTVSQQQSCPTCSTSLLNGEFKVTYDVNRDKLCDLLVANNYFTH FFAPKNLTNMSKNLVFVIDISGSMEGQKVRQTKEALLKILEDMRPVDNFDLVLFGSKVQSWKGSLVPVSN ANLQAAQDFVRRFSLAGATNLNGGLLRGIEILNKAQGSHPELSSPASILIMLTDGEPTEGETDRSQILKN VRNAIRGRFPLYNLGFGHDLDFSFLEVMSTENNGWAQRIYEDHDATQQLQGFYNQVANPLLTDVELQYPQ DAVLALTQHRHKQYYDGSEIVVAGRIANHKLNTFKADVRARGEKQEFRATCLVDEEEMKKLLRERGHVLE NHVERLWAYLTIQELLAKRMKTEGEERANLSSQVLKMSLDYHFVTPLTSLTIRGLTDEDGLEPTIDKPLE DSQPLEMVGPRRTFVLSAIQPSPTAHPIDSKLPLRVTGVDTDPHFIIYVPSKEDSLCFNINEEPGVILNL VQDPDTGFTVNGQLIGNKASSPGQHESTYFGRLGISSPTSDFQLEVTPQNITLNPSSSGSMFSWRDQAVL QKDGVVVTINKKRNLVVSVDDGATFEIVLHRTWKGSAVHQDFLGFYVLDSFRMSARTKGLLGQFFSPLDF EVFDLHPGSDPTKTDATMVVKNRQLTVTRGLQKDYSKDPRHGAEVPCWFVHDNGAGLIDGVHTDYVVSDI F TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC028814 |
| ORF Size | 2733 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC028814.1 |
| RefSeq Size | 2921 bp |
| RefSeq ORF | 2735 bp |
| Locus ID | 16424 |
| MW | 107 kDa |
| Gene Summary | This gene encodes a heavy chain of inter-alpha trypsin inhibitor (IaI) family of plasma serine protease inhibitors. IaI proteins are protein-glycosaminoglycan-protein complexes comprised of two heavy chains and a light chain. The encoded protein covalently associates with the light chain via a chondroitin sulfate moiety. Intravenous administration of the encoded protein improved survival of mice after infection with Escherichia coli. This gene is located adjacent to two other IaI heavy chain genes. Alternative splicing results in multiple transcript variants encoding different isoforms that may undergo similar proteolytic processing to generate mature protein. [provided by RefSeq, Oct 2015] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC220729 | Itih1 (untagged) - Mouse inter-alpha trypsin inhibitor, heavy chain 1 (cDNA clone MGC:36127 IMAGE:5099284), (10ug) |
CNY 9,120.00 |
|
| MG211152 | Itih1 (tGFP-tagged) - Mouse inter-alpha trypsin inhibitor, heavy chain 1 (cDNA clone MGC:36127 IMAGE:5099284) |
CNY 10,070.00 |
|
| MR211152L3 | Lenti ORF clone of Itih1 (Myc-DDK-tagged) - Mouse inter-alpha trypsin inhibitor, heavy chain 1 (cDNA clone MGC:36127 IMAGE:5099284) |
CNY 11,020.00 |
|
| MR211152L4 | Lenti ORF clone of Itih1 (mGFP-tagged) - Mouse inter-alpha trypsin inhibitor, heavy chain 1 (cDNA clone MGC:36127 IMAGE:5099284) |
CNY 11,020.00 |
