Itih1 (BC028814) Mouse Tagged ORF Clone

CAT#: MG211152

  • TrueORF®

Itih1 (tGFP-tagged) - Mouse inter-alpha trypsin inhibitor, heavy chain 1 (cDNA clone MGC:36127 IMAGE:5099284)

ORF Plasmid: DDK tGFP



  "BC028814" in other vectors (4)

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Mouse Protein / Lysate Request

CNY 10,070.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


Itih1 Antibody - middle region
    • 100 ul

CNY 5,250.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag TurboGFP
Synonyms Intin1; ITI-HC1; Itih-1
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MG211152 representing BC028814
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGGGTGCCACAGGGCTCCGGGTGCTGCTGTGCTTGCTGCCGCTCCTCACTCTGCAGGCCAGGCCTG
CTTTGGGCTTGGCAACAGGCAGACCCAGGGGCAGTGAGAAGCGACACGCTGTGGACACTTATTATCTTCA
GTCAGTCAATGGTGTGTCCATCAAGAGCTTGAAAGTCAATTGCAAAGTCACCTCTCGCTTTGCCCACTAC
GTCATCACCAGCCAAGTGGTCAACAATGCTGATAAAGCCAGGGAAGTGGCTTTTGATGTGGAAATCCCCA
AGACAGCCTTCATCAGTGACTTTGCCATAACATCAGATGGGAAGGCGTTCATTGGGGACATAAAGGACAA
AGTGACTGCATGGAAGCAGTACCGGAAGGCAGCCGTTTTAGGGGAGAGCGCTGGCCTTGTCAGAGCCTCG
GGCAGAAATATGGAGCAGTTCACCATCCATATCACTGTTGGAGCCCAGAGCAAGGCCACGTTCCGGCTCA
CCTATGAGGAGGTACTAAAGAGGAGACTGATGCAGTATGACATCACCATCAAAGTCAGACCCAAGCAGCT
GGTACAGCATTTTGAGATCGATGTGGACATCTTTGAACCCCAGGGGATCAGCAAGCTGGACGCTCAGGCC
TCCTTCCTCAGTGAGGAACTGGCTGCTCAAACCATCAAGAAGTCTTTCTCAGGGAAAAAGGGTCATGTGC
TCTTCCGCCCCACTGTGAGCCAGCAGCAGTCCTGCCCCACATGTTCTACATCCTTGCTAAATGGAGAATT
CAAGGTGACCTACGATGTCAATCGAGACAAGCTCTGTGACCTCCTGGTGGCCAACAACTACTTTACACAT
TTCTTTGCCCCCAAAAACCTGACCAACATGAGCAAGAACCTGGTTTTTGTGATTGACATCAGTGGCTCCA
TGGAAGGCCAGAAAGTGAGGCAGACAAAGGAGGCACTCCTTAAGATCCTGGAGGACATGAGGCCAGTAGA
CAACTTTGACCTGGTCCTCTTTGGCTCTAAAGTGCAATCATGGAAGGGCTCCCTGGTACCTGTGTCTAAT
GCCAATCTGCAAGCAGCTCAAGACTTTGTGAGGCGCTTTTCTTTGGCTGGAGCCACAAACCTGAATGGAG
GCTTGCTCCGAGGAATCGAGATCTTAAACAAAGCTCAAGGAAGCCACCCAGAACTCAGCAGCCCGGCCTC
AATTCTTATCATGTTGACAGACGGAGAGCCCACTGAAGGGGAGACGGACCGTTCCCAGATCCTCAAGAAT
GTACGCAACGCTATCCGGGGCAGGTTCCCGCTCTACAACCTCGGCTTTGGCCACGACCTGGACTTCAGCT
TCCTGGAGGTCATGTCCACTGAGAACAACGGATGGGCCCAGAGAATTTATGAGGACCATGATGCCACCCA
ACAGCTACAGGGTTTCTACAATCAAGTAGCCAATCCCCTGCTGACAGATGTGGAACTGCAGTACCCCCAG
GATGCTGTCTTGGCTCTAACCCAGCACCGACATAAACAGTACTATGACGGCTCGGAGATTGTGGTGGCTG
GGCGCATTGCTAACCACAAACTGAACACCTTTAAGGCTGACGTTCGGGCTCGTGGGGAAAAGCAAGAATT
CAGGGCAACCTGCCTAGTGGACGAGGAAGAGATGAAGAAACTGCTCCGAGAGCGTGGCCACGTGCTAGAG
AATCATGTGGAGAGGCTCTGGGCCTACCTCACGATCCAGGAGCTCCTGGCTAAGCGGATGAAGACGGAAG
GGGAGGAGAGGGCCAACCTGTCATCCCAGGTCCTGAAGATGTCACTGGACTATCATTTCGTAACGCCACT
GACCTCTCTGACTATCAGAGGCCTGACAGACGAGGATGGGCTGGAGCCCACCATTGACAAGCCCCTGGAG
GATTCTCAGCCCTTAGAGATGGTGGGACCCAGAAGAACGTTTGTGCTGTCAGCCATACAGCCATCTCCTA
CAGCCCACCCCATAGACTCAAAGTTGCCACTCCGTGTGACAGGTGTGGACACCGATCCCCACTTCATCAT
CTATGTGCCCTCAAAAGAGGATAGCTTGTGCTTCAACATCAATGAGGAACCTGGTGTGATCCTGAACCTA
GTACAGGACCCTGACACAGGCTTCACCGTGAATGGGCAGCTCATTGGGAACAAGGCCAGCAGCCCTGGGC
AACACGAGAGCACATACTTCGGGAGATTGGGAATCTCAAGTCCCACATCAGACTTTCAGCTGGAAGTGAC
TCCTCAGAACATTACACTGAACCCCAGCTCCAGCGGGTCCATGTTCTCCTGGAGGGACCAGGCTGTGCTG
CAGAAGGATGGGGTAGTGGTGACAATCAACAAGAAGAGGAACCTGGTGGTGTCTGTGGATGATGGGGCTA
CCTTTGAGATTGTCCTGCACAGAACATGGAAGGGGAGTGCAGTCCACCAGGACTTCCTGGGTTTCTATGT
GCTGGACAGCTTCCGGATGTCAGCCCGGACAAAGGGGCTACTGGGACAATTCTTCTCCCCACTGGATTTT
GAAGTATTTGACCTCCACCCAGGCTCTGACCCTACAAAGACAGATGCCACAATGGTGGTGAAGAATCGGC
AGCTGACAGTCACCCGAGGGTTGCAAAAAGACTACAGCAAGGACCCCAGACATGGAGCAGAAGTGCCTTG
CTGGTTTGTCCACGACAATGGAGCTGGACTGATTGATGGTGTTCACACTGACTATGTCGTCTCTGACATC
TTC


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>MG211152 representing BC028814
Red=Cloning site Green=Tags(s)

MEGATGLRVLLCLLPLLTLQARPALGLATGRPRGSEKRHAVDTYYLQSVNGVSIKSLKVNCKVTSRFAHY
VITSQVVNNADKAREVAFDVEIPKTAFISDFAITSDGKAFIGDIKDKVTAWKQYRKAAVLGESAGLVRAS
GRNMEQFTIHITVGAQSKATFRLTYEEVLKRRLMQYDITIKVRPKQLVQHFEIDVDIFEPQGISKLDAQA
SFLSEELAAQTIKKSFSGKKGHVLFRPTVSQQQSCPTCSTSLLNGEFKVTYDVNRDKLCDLLVANNYFTH
FFAPKNLTNMSKNLVFVIDISGSMEGQKVRQTKEALLKILEDMRPVDNFDLVLFGSKVQSWKGSLVPVSN
ANLQAAQDFVRRFSLAGATNLNGGLLRGIEILNKAQGSHPELSSPASILIMLTDGEPTEGETDRSQILKN
VRNAIRGRFPLYNLGFGHDLDFSFLEVMSTENNGWAQRIYEDHDATQQLQGFYNQVANPLLTDVELQYPQ
DAVLALTQHRHKQYYDGSEIVVAGRIANHKLNTFKADVRARGEKQEFRATCLVDEEEMKKLLRERGHVLE
NHVERLWAYLTIQELLAKRMKTEGEERANLSSQVLKMSLDYHFVTPLTSLTIRGLTDEDGLEPTIDKPLE
DSQPLEMVGPRRTFVLSAIQPSPTAHPIDSKLPLRVTGVDTDPHFIIYVPSKEDSLCFNINEEPGVILNL
VQDPDTGFTVNGQLIGNKASSPGQHESTYFGRLGISSPTSDFQLEVTPQNITLNPSSSGSMFSWRDQAVL
QKDGVVVTINKKRNLVVSVDDGATFEIVLHRTWKGSAVHQDFLGFYVLDSFRMSARTKGLLGQFFSPLDF
EVFDLHPGSDPTKTDATMVVKNRQLTVTRGLQKDYSKDPRHGAEVPCWFVHDNGAGLIDGVHTDYVVSDI
F

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC028814
ORF Size 2733 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC028814.1
RefSeq Size 2921 bp
RefSeq ORF 2735 bp
Locus ID 16424
Gene Summary This gene encodes a heavy chain of inter-alpha trypsin inhibitor (IaI) family of plasma serine protease inhibitors. IaI proteins are protein-glycosaminoglycan-protein complexes comprised of two heavy chains and a light chain. The encoded protein covalently associates with the light chain via a chondroitin sulfate moiety. Intravenous administration of the encoded protein improved survival of mice after infection with Escherichia coli. This gene is located adjacent to two other IaI heavy chain genes. Alternative splicing results in multiple transcript variants encoding different isoforms that may undergo similar proteolytic processing to generate mature protein. [provided by RefSeq, Oct 2015]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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