Pld2 (BC068317) Mouse Tagged ORF Clone

CAT#: MR211273

  • TrueORF®

Pld2 (Myc-DDK-tagged) - Mouse phospholipase D2 (cDNA clone MGC:76743 IMAGE:30094606)

ORF Plasmid: DDK tGFP



  "BC068317" in other vectors (4)

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Mouse Protein / Lysate Request

CNY 6,912.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Synonyms PLD1C
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR211273 representing BC068317
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGACTGTAACCCAGAAGAACCTCTTTCCCTATGGGGACTATCTGAACTCCAGCCAGTTGCACATGGAGC
CAGATGAGGTTGACACTCTGAGGGAAGGAGAGGATCCAGCTGATCGAATGCATCCCTATCTGGCCATCTA
TGACCTTCAGCCTCTGAAAGCACACCCCTTGGTGTTCGCCCCTGGGGTCCCTGTTATAGCCCAGGTGGTG
GGCACCGAAAGATACACCAGCGGATCCAAGGTGGGAACCTGTACTCTATATTCTGTTCGCTTGACGCATG
GTGACTTTACCTGGACAACCAAGAAGAAGTTCCGACACTTTCAGGAGCTGCATCGGGACCTCCAGAGACA
CAAAGTCTTGATGAGTCTGCTCCCTTTGGCTCGCTTTGCTGTGACCCATTCTCCAGCCCGAGAGGCAGCC
GCCGAGGATATACCCTCCCTACCCCGAGGAGGTTCTGAGGGCTCTGCCAGACACACAGCCAGCAAACAGA
AATACTTGGAAAATTACCTCAACCGCCTCCTGACCATGTCTTTCTATCGCAATTACCACGCCATGACAGA
ATTTCTGGAAGTCAGTCAACTTTCCTTTATCCCAGACCTTGGCTCCAAAGGACTGGAAGGGGTGATCCGG
AAGCGCTCGGGCGGGCATCGAGTTCCCGGCTTCACCTTCTGTGGCCGAGACCAAGTTTGTTATCGATGGT
CCAAGAGGTGGCTGGTGGTGAAGGACTCCTTCCTGCTGTACATGCGCCCGGAGACCGGCGCCATCTCATT
TGTTCAGCTTTTTGACCCTGGCTTTGAGGTCCAGGTCGGAAAAAGGAGCACAGAGACGCGGTATGGGGTG
AGGATCGACACCTCCCACAGGTCCCTGATTCTCAAATGCAGCAGCTACCGGCAGGCACGGTGGTGGGGCC
AGGAGATCACGGAGCTGGCACAGGGTTCGGGCAGAGATTTTCTACAGCTACATCAGCATGACAGCTATGC
CCCACCCCGGCCCGGCACCCTGGCCCGGTGGTTTGTGAATGGGGCAGGTTACTTTGCTGCTGTGGCAGAT
GCCATCCTGCGAGCTCAAGAGGAGATTTTCATCACAGACTGGTGGTTGAGTCCTGAAATTTACCTGAAGC
GTCCAGCCCATTCCGACGACTGGAGACTGGACATTATGCTCAAGAGGAAGGCGGAAGAAGGTGTCCGAGT
TTCCATACTGCTGTTTAAGGAAGTGGAGCTGGCCTTGGGCATCAACAGTGGCTACAGCAAGAGGACGCTG
ATGCTGCTGCATCCCAACATAAAGGTGATGCGACACCCAGACCTTGTGACACTGTGGGCTCATCACGAGA
AGCTCCTGGTGGTAGACCAAGTGGTGGCATTCTTGGGCGGGCTGGACCTGGCCTTCGGCCGCTGGGATGA
CGTGCAATACCGACTGACTGACCTGGGTGACCCCTCTGAACCTGTACATTTACAGACTCCCACACTAGGT
TCAGACCCTGCAGCCACTCCAGACCTCTCGCATAACCAATTCTTCTGGCTGGGAAAGGACTACAGCAACC
TCATCACCAAGGACTGGGTGCAGCTGGACCGGCCTTTTGAAGATTTCATCGACAGGGAGACCACACCCAG
GATGCCATGGAGGGATGTTGGAGTGGTTGTACACGGAGTAGCTGCCAGGGACCTTGCCCGGCACTTCATC
CAGCGCTGGAATTTCACCAAGACCACCAAGGCCAGGTATAAGACACCTTTGTACCCCTACCTGCTGCCCA
AGTCCACCAGCACTGCAAACAATCTCCCCTTCATGATCCCAGGCGGGCAGTGTGCCACTGTGCAGGTCTT
GAGGTCTGTGGATCGATGGTCAGCAGGGACATTGGAGAACTCCATCCTCAATGCCTACCTACATACCATT
CGAGAGAGCCAGCACTTTCTCTACATTGAGAATCAGTTCTTCATTAGCTGCTCAGATGGGCGAACAGTTC
TGAACAAGGTGGGCGATGAGATTGTGGACAGAATCCTGAAGGCTCACGAACAGGGGCAGTGTTTCCGAGT
CTACTTGCTTCTGCCTTTGCTCCCTGGCTTTGAGGGGGACATCTCCACAGGGGGTGGTAACTCCATCCAG
GCTATTCTGCACTTCACCTACAGCCTCTGCCTCCTTCACCCTTTCTTCTCTCTCAGGACCCTGTGTCGTG
GGGAACATTCAATCCTACATCGTCTCAAAGCAGCCATGGGGACTGCGTGGCGAGATTACATGTCCATCTG
TGGGCTTCGCACCCATGGAGAGCTGGGCGGGCACCCAATCTCTGAGCTCATCTATATCCACAGCAAGATG
CTCATTGCGGATGACAGAACAGTCATCATTGGTTCTGCGAACATCAATGACAGGAGCTTGCTGGGGAAGC
GTGACAGTGAGCTAGCCATCCTGATCAAGGACACAGAAATGGAACCATCCCTCATGGATGGGGTGGAGTA
CCAGGCAGGCAGATTTGCCTTGAGTTTGCGGAAGCACTGTTTCAGTGTGATTCTTGGGGCAAATACCTGG
CCAGACCTGGATCTCCGAGACCCTGTCTGTGATGACTTCTTCCAGCTGTGGCAAGAAACAGCGGAGAACA
ATGCCACCATCTATGAGCAGATCTTCCGCTGCCTGCCGTCCAATGCTACCCGTTCCCTGCGGGCTCTCCG
GGAGTATGTGGCTGTGGAGTCCTTGGCTACAGTCAGCCCTTCTTTGGCTCAGTCTGAGCTTGCCCACATC
CAGGGCCACCTAGTTCACTTCCCCCTCAAGTTTCTGGAGGACGAGTCCTTGTTGCCCCCACTGGGGAGTA
AAGAAGGGATGATACCTTTAGAAGTGTGGACA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR211273 representing BC068317
Red=Cloning site Green=Tags(s)

MTVTQKNLFPYGDYLNSSQLHMEPDEVDTLREGEDPADRMHPYLAIYDLQPLKAHPLVFAPGVPVIAQVV
GTERYTSGSKVGTCTLYSVRLTHGDFTWTTKKKFRHFQELHRDLQRHKVLMSLLPLARFAVTHSPAREAA
AEDIPSLPRGGSEGSARHTASKQKYLENYLNRLLTMSFYRNYHAMTEFLEVSQLSFIPDLGSKGLEGVIR
KRSGGHRVPGFTFCGRDQVCYRWSKRWLVVKDSFLLYMRPETGAISFVQLFDPGFEVQVGKRSTETRYGV
RIDTSHRSLILKCSSYRQARWWGQEITELAQGSGRDFLQLHQHDSYAPPRPGTLARWFVNGAGYFAAVAD
AILRAQEEIFITDWWLSPEIYLKRPAHSDDWRLDIMLKRKAEEGVRVSILLFKEVELALGINSGYSKRTL
MLLHPNIKVMRHPDLVTLWAHHEKLLVVDQVVAFLGGLDLAFGRWDDVQYRLTDLGDPSEPVHLQTPTLG
SDPAATPDLSHNQFFWLGKDYSNLITKDWVQLDRPFEDFIDRETTPRMPWRDVGVVVHGVAARDLARHFI
QRWNFTKTTKARYKTPLYPYLLPKSTSTANNLPFMIPGGQCATVQVLRSVDRWSAGTLENSILNAYLHTI
RESQHFLYIENQFFISCSDGRTVLNKVGDEIVDRILKAHEQGQCFRVYLLLPLLPGFEGDISTGGGNSIQ
AILHFTYSLCLLHPFFSLRTLCRGEHSILHRLKAAMGTAWRDYMSICGLRTHGELGGHPISELIYIHSKM
LIADDRTVIIGSANINDRSLLGKRDSELAILIKDTEMEPSLMDGVEYQAGRFALSLRKHCFSVILGANTW
PDLDLRDPVCDDFFQLWQETAENNATIYEQIFRCLPSNATRSLRALREYVAVESLATVSPSLAQSELAHI
QGHLVHFPLKFLEDESLLPPLGSKEGMIPLEVWT

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC068317
ORF Size 2832 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC068317.1
RefSeq Size 3671 bp
RefSeq ORF 2834 bp
Locus ID 18806
MW 134.5 kDa
Gene Summary This gene is a member of the phospholipase D (PLD) superfamily. The encoded protein catalyzes the hydrolysis of phosphatidylcholine to phosphatidic acid and choline. Phosphatidic acid is an essential intracellular lipid second messenger for many signaling pathways and has been implicated in a variety of physiological processes including cytoskeletal organization and cell proliferation. A similar gene in human may also function as a guanine nucleotide exchange factor (GEF) for the small GTPase Rac2. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2014]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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