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Pld2 (BC068317) Mouse Tagged ORF Clone
CAT＃: MR211273
- TrueORF®
Pld2 (Myc-DDK-tagged) - Mouse phospholipase D2 (cDNA clone MGC:76743 IMAGE:30094606)
ORF Plasmid: tGFP
"BC068317" in other vectors (4)
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CNY 6,912.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | PLD1C |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR211273 representing BC068317
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACTGTAACCCAGAAGAACCTCTTTCCCTATGGGGACTATCTGAACTCCAGCCAGTTGCACATGGAGC CAGATGAGGTTGACACTCTGAGGGAAGGAGAGGATCCAGCTGATCGAATGCATCCCTATCTGGCCATCTA TGACCTTCAGCCTCTGAAAGCACACCCCTTGGTGTTCGCCCCTGGGGTCCCTGTTATAGCCCAGGTGGTG GGCACCGAAAGATACACCAGCGGATCCAAGGTGGGAACCTGTACTCTATATTCTGTTCGCTTGACGCATG GTGACTTTACCTGGACAACCAAGAAGAAGTTCCGACACTTTCAGGAGCTGCATCGGGACCTCCAGAGACA CAAAGTCTTGATGAGTCTGCTCCCTTTGGCTCGCTTTGCTGTGACCCATTCTCCAGCCCGAGAGGCAGCC GCCGAGGATATACCCTCCCTACCCCGAGGAGGTTCTGAGGGCTCTGCCAGACACACAGCCAGCAAACAGA AATACTTGGAAAATTACCTCAACCGCCTCCTGACCATGTCTTTCTATCGCAATTACCACGCCATGACAGA ATTTCTGGAAGTCAGTCAACTTTCCTTTATCCCAGACCTTGGCTCCAAAGGACTGGAAGGGGTGATCCGG AAGCGCTCGGGCGGGCATCGAGTTCCCGGCTTCACCTTCTGTGGCCGAGACCAAGTTTGTTATCGATGGT CCAAGAGGTGGCTGGTGGTGAAGGACTCCTTCCTGCTGTACATGCGCCCGGAGACCGGCGCCATCTCATT TGTTCAGCTTTTTGACCCTGGCTTTGAGGTCCAGGTCGGAAAAAGGAGCACAGAGACGCGGTATGGGGTG AGGATCGACACCTCCCACAGGTCCCTGATTCTCAAATGCAGCAGCTACCGGCAGGCACGGTGGTGGGGCC AGGAGATCACGGAGCTGGCACAGGGTTCGGGCAGAGATTTTCTACAGCTACATCAGCATGACAGCTATGC CCCACCCCGGCCCGGCACCCTGGCCCGGTGGTTTGTGAATGGGGCAGGTTACTTTGCTGCTGTGGCAGAT GCCATCCTGCGAGCTCAAGAGGAGATTTTCATCACAGACTGGTGGTTGAGTCCTGAAATTTACCTGAAGC GTCCAGCCCATTCCGACGACTGGAGACTGGACATTATGCTCAAGAGGAAGGCGGAAGAAGGTGTCCGAGT TTCCATACTGCTGTTTAAGGAAGTGGAGCTGGCCTTGGGCATCAACAGTGGCTACAGCAAGAGGACGCTG ATGCTGCTGCATCCCAACATAAAGGTGATGCGACACCCAGACCTTGTGACACTGTGGGCTCATCACGAGA AGCTCCTGGTGGTAGACCAAGTGGTGGCATTCTTGGGCGGGCTGGACCTGGCCTTCGGCCGCTGGGATGA CGTGCAATACCGACTGACTGACCTGGGTGACCCCTCTGAACCTGTACATTTACAGACTCCCACACTAGGT TCAGACCCTGCAGCCACTCCAGACCTCTCGCATAACCAATTCTTCTGGCTGGGAAAGGACTACAGCAACC TCATCACCAAGGACTGGGTGCAGCTGGACCGGCCTTTTGAAGATTTCATCGACAGGGAGACCACACCCAG GATGCCATGGAGGGATGTTGGAGTGGTTGTACACGGAGTAGCTGCCAGGGACCTTGCCCGGCACTTCATC CAGCGCTGGAATTTCACCAAGACCACCAAGGCCAGGTATAAGACACCTTTGTACCCCTACCTGCTGCCCA AGTCCACCAGCACTGCAAACAATCTCCCCTTCATGATCCCAGGCGGGCAGTGTGCCACTGTGCAGGTCTT GAGGTCTGTGGATCGATGGTCAGCAGGGACATTGGAGAACTCCATCCTCAATGCCTACCTACATACCATT CGAGAGAGCCAGCACTTTCTCTACATTGAGAATCAGTTCTTCATTAGCTGCTCAGATGGGCGAACAGTTC TGAACAAGGTGGGCGATGAGATTGTGGACAGAATCCTGAAGGCTCACGAACAGGGGCAGTGTTTCCGAGT CTACTTGCTTCTGCCTTTGCTCCCTGGCTTTGAGGGGGACATCTCCACAGGGGGTGGTAACTCCATCCAG GCTATTCTGCACTTCACCTACAGCCTCTGCCTCCTTCACCCTTTCTTCTCTCTCAGGACCCTGTGTCGTG GGGAACATTCAATCCTACATCGTCTCAAAGCAGCCATGGGGACTGCGTGGCGAGATTACATGTCCATCTG TGGGCTTCGCACCCATGGAGAGCTGGGCGGGCACCCAATCTCTGAGCTCATCTATATCCACAGCAAGATG CTCATTGCGGATGACAGAACAGTCATCATTGGTTCTGCGAACATCAATGACAGGAGCTTGCTGGGGAAGC GTGACAGTGAGCTAGCCATCCTGATCAAGGACACAGAAATGGAACCATCCCTCATGGATGGGGTGGAGTA CCAGGCAGGCAGATTTGCCTTGAGTTTGCGGAAGCACTGTTTCAGTGTGATTCTTGGGGCAAATACCTGG CCAGACCTGGATCTCCGAGACCCTGTCTGTGATGACTTCTTCCAGCTGTGGCAAGAAACAGCGGAGAACA ATGCCACCATCTATGAGCAGATCTTCCGCTGCCTGCCGTCCAATGCTACCCGTTCCCTGCGGGCTCTCCG GGAGTATGTGGCTGTGGAGTCCTTGGCTACAGTCAGCCCTTCTTTGGCTCAGTCTGAGCTTGCCCACATC CAGGGCCACCTAGTTCACTTCCCCCTCAAGTTTCTGGAGGACGAGTCCTTGTTGCCCCCACTGGGGAGTA AAGAAGGGATGATACCTTTAGAAGTGTGGACA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR211273 representing BC068317
Red=Cloning site Green=Tags(s) MTVTQKNLFPYGDYLNSSQLHMEPDEVDTLREGEDPADRMHPYLAIYDLQPLKAHPLVFAPGVPVIAQVV GTERYTSGSKVGTCTLYSVRLTHGDFTWTTKKKFRHFQELHRDLQRHKVLMSLLPLARFAVTHSPAREAA AEDIPSLPRGGSEGSARHTASKQKYLENYLNRLLTMSFYRNYHAMTEFLEVSQLSFIPDLGSKGLEGVIR KRSGGHRVPGFTFCGRDQVCYRWSKRWLVVKDSFLLYMRPETGAISFVQLFDPGFEVQVGKRSTETRYGV RIDTSHRSLILKCSSYRQARWWGQEITELAQGSGRDFLQLHQHDSYAPPRPGTLARWFVNGAGYFAAVAD AILRAQEEIFITDWWLSPEIYLKRPAHSDDWRLDIMLKRKAEEGVRVSILLFKEVELALGINSGYSKRTL MLLHPNIKVMRHPDLVTLWAHHEKLLVVDQVVAFLGGLDLAFGRWDDVQYRLTDLGDPSEPVHLQTPTLG SDPAATPDLSHNQFFWLGKDYSNLITKDWVQLDRPFEDFIDRETTPRMPWRDVGVVVHGVAARDLARHFI QRWNFTKTTKARYKTPLYPYLLPKSTSTANNLPFMIPGGQCATVQVLRSVDRWSAGTLENSILNAYLHTI RESQHFLYIENQFFISCSDGRTVLNKVGDEIVDRILKAHEQGQCFRVYLLLPLLPGFEGDISTGGGNSIQ AILHFTYSLCLLHPFFSLRTLCRGEHSILHRLKAAMGTAWRDYMSICGLRTHGELGGHPISELIYIHSKM LIADDRTVIIGSANINDRSLLGKRDSELAILIKDTEMEPSLMDGVEYQAGRFALSLRKHCFSVILGANTW PDLDLRDPVCDDFFQLWQETAENNATIYEQIFRCLPSNATRSLRALREYVAVESLATVSPSLAQSELAHI QGHLVHFPLKFLEDESLLPPLGSKEGMIPLEVWT TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC068317 |
| ORF Size | 2832 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC068317.1 |
| RefSeq Size | 3671 bp |
| RefSeq ORF | 2834 bp |
| Locus ID | 18806 |
| MW | 134.5 kDa |
| Gene Summary | This gene is a member of the phospholipase D (PLD) superfamily. The encoded protein catalyzes the hydrolysis of phosphatidylcholine to phosphatidic acid and choline. Phosphatidic acid is an essential intracellular lipid second messenger for many signaling pathways and has been implicated in a variety of physiological processes including cytoskeletal organization and cell proliferation. A similar gene in human may also function as a guanine nucleotide exchange factor (GEF) for the small GTPase Rac2. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2014] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC220747 | Pld2 (untagged) - Mouse phospholipase D2 (cDNA clone MGC:76743 IMAGE:30094606), (10ug) |
CNY 9,120.00 |
|
| MG211273 | Pld2 (tGFP-tagged) - Mouse phospholipase D2 (cDNA clone MGC:76743 IMAGE:30094606) |
CNY 5,990.00 |
|
| MR211273L3 | Lenti ORF clone of Pld2 (Myc-DDK-tagged) - Mouse phospholipase D2 (cDNA clone MGC:76743 IMAGE:30094606) |
CNY 7,320.00 |
|
| MR211273L4 | Lenti ORF clone of Pld2 (mGFP-tagged) - Mouse phospholipase D2 (cDNA clone MGC:76743 IMAGE:30094606) |
CNY 7,320.00 |
