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Gabbr1 (NM_019439) Mouse Tagged ORF Clone
CAT＃: MR211314
- TrueORF®
Gabbr1 (Myc-DDK-tagged) - Mouse gamma-aminobutyric acid (GABA) B receptor, 1 (Gabbr1)
ORF Plasmid: tGFP
"NM_019439" in other vectors (4)
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CNY 7,736.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | bM573K1.1; GABAB1; GABAbR1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR211314 representing NM_019439
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTGCTGCTGCTGCTGGTGCCTCTCTTCCTCCGCCCCCTGGGCGCTGGCGGGGCTCAGACCCCCAACG TCACCTCGGAAGGTTGCCAGATTATACATCCGCCCTGGGAAGGTGGCATCAGGTACCGTGGCTTGACTCG CGACCAGGTGAAGGCCATCAATTTCCTGCCTGTGGACTATGAGATTGAATATGTGTGCCGGGGCGAACGC GAGGTGGTGGGGCCCAAGGTGCGCAAGTGCCTGGCCAACGGCTCCTGGACGGATATGGACACACCCAGTC GCTGTGTCCGAATCTGCTCCAAGTCTTATTTGACCCTGGAAAATGGGAAGGTTTTCCTGACGGGTGGGGA CCTCCCAGCTCTGGATGGAGCCCGGGTGGATTTCCGATGTGACCCCGACTTCCATCTGGTGGGCAGCTCC CGGAGCATCTGTAGTCAGGGCCAGTGGAGCACCCCCAAGCCCCACTGCCAGGTGAATCGAACGCCACACT CAGAACGGCGTGCAGTATACATCGGGGCGCTGTTTCCCATGAGCGGGGGCTGGCCGGGGGGCCAGGCCTG CCAGCCTGCGGTGGAGATGGCGCTGGAGGACGTTAACAGCCGCAGAGACATCCTGCCGGACTACGAGCTC AAGCTTATCCACCACGACAGCAAGTGCGACCCAGGGCAAGCCACCAAGTACTTGTATGAACTACTCTACA ACGACCCCATCAAGATCATCCTCATGCCCGGCTGCAGCTCTGTGTCCACACTGGTAGCCGAGGCTGCCCG GATGTGGAACCTTATTGTGCTCTCATATGGCTCCAGCTCACCAGCCTTGTCAAACCGACAGCGGTTTCCA ACGTTCTTTCGGACACATCCATCCGCCACACTCCACAATCCCACCCGGGTGAAACTCTTCGAAAAGTGGG GCTGGAAGAAGATTGCCACCATCCAGCAGACTACCGAGGTCTTCACCTCAACACTGGATGACCTGGAGGA GCGAGTGAAAGAGGCTGGGATTGAGATCACTTTTCGACAGAGTTTCTTCTCAGATCCAGCTGTGCCTGTT AAAAACCTGAAGCGTCAAGATGCTCGAATCATCGTGGGACTTTTCTATGAGACCGAAGCCCGGAAAGTTT TTTGTGAGGTCTATAAGGAACGGCTCTTTGGGAAGAAGTATGTCTGGTTTCTCATCGGGTGGTATGCTGA CAACTGGTTCAAAACCTATGACCCGTCAATCAATTGTACAGTAGAAGAGATGACTGAGGCGGTGGAGGGC CATATCACCACGGAGATTGTCATGCTGAACCCTGCCAACACCCGAAGCATTTCCAACATGACATCACAGG AATTTGTGGAGAAACTAACCAAGCGGCTGAAAAGACACCCTGAGGAGACTGGAGGCTTCCAGGAGGCACC ACTGGCCTATGATGCTATCTGGGCCTTGGCTTTGGCCTTGAACAAGACCTCTGGAGGAGGTGGCCGTTCA GGAGTGCGCCTGGAGGACTTTAACTACAACAACCAGACCATTACAGACCAAATCTACCGGGCCATGAACT CCTCCTCCTTTGAGGGTGTTTCTGGCCACGTGGTCTTTGATGCCAGCGGCTCCCGGATGGCATGGACGCT TATCGAGCAGCTACAGGGCGGCAGCTACAAGAAGATCGGCTACTACGACAGCACCAAGGATGATCTTTCC TGGTCCAAAACAGACAAGTGGATCGGAGGGTCTCCCCCAGCCGACCAGACCTTGGTCATCAAGACATTCC GTTTCCTGTCACAGAAACTCTTTATCTCCGTCTCAGTTCTCTCCAGCCTGGGCATTGTTCTTGCTGTTGT CTGTCTGTCCTTTAACATCTACAACTCCCACGTTCGTTATATCCAGAACTCCCAGCCCAACCTGAACAAT CTGACTGCTGTGGGCTGCTCACTGGCACTAGCTGCTGTCTTCCCCCTTGGGCTGGATGGTTACCACATAG GGAGAAGCCAGTTCCCATTTGTCTGCCAGGCCCGCCTTTGGCTCTTGGGCTTAGGCTTTAGTCTGGGCTA TGGCTCTATGTTCACCAAGATCTGGTGGGTCCACACAGTCTTCACAAAGAAGGAGGAGAAGAAGGAATGG AGGAAGACCCTAGAGCCTTGGAAACTGTACGCCACTGTAGGCCTGCTGGTGGGCATGGATATCCTGACTC TTGCCATCTGGCAGATTGTGGACCCCTTGCACCGAACCATTGAGACTTTTGCCAAGGAGGAACCAAAGGA AGACATTGATGTCTCCATTCTGCCCCAGCTGGAGCACTGCAGCTCCAAGAAGATGAATACATGGCTCGGC ATTTTCTATGGTTACAAAGGGCTGCTGCTGCTGCTGGGAATCTTTCTTGCTTATGAGACCAAAAGTGTGT CCACTGAAAAGATCAATGACCACAGGGCCGTGGGCATGGCTATCTACAATGTTGCGGTCCTGTGTCTCAT TACCGCTCCTGTCACCATGATCCTTTCCAGCCAGCAGGACGCAGCCTTCGCCTTTGCCTCTCTGGCCATT GTGTTCTCTTCCTACATTACTCTGGTTGTGCTCTTTGTGCCTAAGATGCGCAGGTTGATCACTCGAGGTG AATGGCAGTCTGAAGCACAGGACACAATGAAAACAGGATCATCCACCAACAACAATGAGGAAGAGAAGTC CCGACTGTTGGAGAAGGAAAACCGTGAATTGGAAAAGATCATCGCCGAGAAAGAGGAGCGTGTCTCTGAA CTGCGCCATCAGCTCCAGTCTCGGCAGCAGATCCGCTCCCGGCGCCACCCCCCAACACCCCCAGACCCCT CTGGGGGCCTTCCCAGGGGACCCTCAGAGCCCCCTGACCGGCTTAGCTGTGATGGGAGTCGAGTACATTT GCTTTACAAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR211314 representing NM_019439
Red=Cloning site Green=Tags(s) MLLLLLVPLFLRPLGAGGAQTPNVTSEGCQIIHPPWEGGIRYRGLTRDQVKAINFLPVDYEIEYVCRGER EVVGPKVRKCLANGSWTDMDTPSRCVRICSKSYLTLENGKVFLTGGDLPALDGARVDFRCDPDFHLVGSS RSICSQGQWSTPKPHCQVNRTPHSERRAVYIGALFPMSGGWPGGQACQPAVEMALEDVNSRRDILPDYEL KLIHHDSKCDPGQATKYLYELLYNDPIKIILMPGCSSVSTLVAEAARMWNLIVLSYGSSSPALSNRQRFP TFFRTHPSATLHNPTRVKLFEKWGWKKIATIQQTTEVFTSTLDDLEERVKEAGIEITFRQSFFSDPAVPV KNLKRQDARIIVGLFYETEARKVFCEVYKERLFGKKYVWFLIGWYADNWFKTYDPSINCTVEEMTEAVEG HITTEIVMLNPANTRSISNMTSQEFVEKLTKRLKRHPEETGGFQEAPLAYDAIWALALALNKTSGGGGRS GVRLEDFNYNNQTITDQIYRAMNSSSFEGVSGHVVFDASGSRMAWTLIEQLQGGSYKKIGYYDSTKDDLS WSKTDKWIGGSPPADQTLVIKTFRFLSQKLFISVSVLSSLGIVLAVVCLSFNIYNSHVRYIQNSQPNLNN LTAVGCSLALAAVFPLGLDGYHIGRSQFPFVCQARLWLLGLGFSLGYGSMFTKIWWVHTVFTKKEEKKEW RKTLEPWKLYATVGLLVGMDILTLAIWQIVDPLHRTIETFAKEEPKEDIDVSILPQLEHCSSKKMNTWLG IFYGYKGLLLLLGIFLAYETKSVSTEKINDHRAVGMAIYNVAVLCLITAPVTMILSSQQDAAFAFASLAI VFSSYITLVVLFVPKMRRLITRGEWQSEAQDTMKTGSSTNNNEEEKSRLLEKENRELEKIIAEKEERVSE LRHQLQSRQQIRSRRHPPTPPDPSGGLPRGPSEPPDRLSCDGSRVHLLYK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_019439 |
| ORF Size | 2880 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_019439.3, NP_062312.3 |
| RefSeq Size | 4486 bp |
| RefSeq ORF | 2883 bp |
| Locus ID | 54393 |
| UniProt ID | Q9WV18 |
| MW | 108.7 kDa |
| Gene Summary | Component of a heterodimeric G-protein coupled receptor for GABA, formed by GABBR1 and GABBR2 (PubMed:10773016, PubMed:10075644). Within the heterodimeric GABA receptor, only GABBR1 seems to bind agonists, while GABBR2 mediates coupling to G proteins (By similarity). Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase (PubMed:10773016, PubMed:10075644). Signaling inhibits adenylate cyclase, stimulates phospholipase A2, activates potassium channels, inactivates voltage-dependent calcium-channels and modulates inositol phospholipid hydrolysis (PubMed:10075644). Calcium is required for high affinity binding to GABA (By similarity). Plays a critical role in the fine-tuning of inhibitory synaptic transmission (By similarity). Pre-synaptic GABA receptor inhibits neurotransmitter release by down-regulating high-voltage activated calcium channels, whereas postsynaptic GABA receptor decreases neuronal excitability by activating a prominent inwardly rectifying potassium (Kir) conductance that underlies the late inhibitory postsynaptic potentials (PubMed:10075644). Not only implicated in synaptic inhibition but also in hippocampal long-term potentiation, slow wave sleep, muscle relaxation and antinociception (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC222832 | Gabbr1 (untagged) - Mouse gamma-aminobutyric acid (GABA) B receptor, 1 (Gabbr1), (10ug) |
CNY 12,260.00 |
|
| MG211314 | Gabbr1 (tGFP-tagged) - Mouse gamma-aminobutyric acid (GABA-B) receptor, 1 (Gabbr1) |
CNY 7,890.00 |
|
| MR211314L3 | Lenti ORF clone of Gabbr1 (Myc-DDK-tagged) - Mouse gamma-aminobutyric acid (GABA) B receptor, 1 (Gabbr1) |
CNY 9,030.00 |
|
| MR211314L4 | Lenti ORF clone of Gabbr1 (mGFP-tagged) - Mouse gamma-aminobutyric acid (GABA) B receptor, 1 (Gabbr1) |
CNY 9,030.00 |
