1500010J02Rik (BC060629) Mouse Tagged ORF Clone

CAT#: MR211335

  • TrueORF®

1500010J02Rik (Myc-DDK-tagged) - Mouse RIKEN cDNA 1500010J02 gene (cDNA clone MGC:79217 IMAGE:6847619)

ORF Plasmid: DDK tGFP



  "BC060629" in other vectors (3)

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Mouse Protein / Lysate Request

CNY 9,600.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Synonyms RP23-19I2.2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR211335 representing BC060629
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGACACTTGGTGAATTAGCCCAAGCTGGCAGCCAGGTGTCCATCATTGTACAGATCCCTGCTCAGATGG
TGTGGCACCGAGTCCTCCGGCCTGGCAGGGCCTATGTACTGACAAAATTGCAAGTAACCAAGACCCGGAT
TCACCTTTCCTGTATTTGGACAACCATTCCATCTTCAACTCTGAAGCCTCTGAGACCAGGTTATGTGCAA
GAGCTGGAGCTGGACTTAGAGTTCTCGAAAGCTGACCTTAAACCACCACCCCAGCCCACCAGCTCCAAAG
ACAGCAGAGGGCAAGAAGGTCTTGTCCGGGCTTCCAAAGTCTTGCATTATTTGGGAACAGTCACTGCTGT
GTTGCATGAGTCTGCCGGCCTCTACATTCTAGATGGGCAGCTGATTCTCTGCCTCGCATACCAGAAGATC
CATGGCCTCAGGCGGGTTATTCGACCAGGGGTCTGTCTGGAGCTCCGAGATGTTCATCTCCTCCAGGCAG
TGGGTGGAGCAACAACAAAACCTGTCCTGGCGCTCTGTCTCCATGGCACTGTTCGGCTTCAAGGCTTTTC
TTGTCTTAAGCCTCTGACTTTGCCGTCCTCCAAAGTTTATGGTGCCTCCTTGTATGAGCAACTCGTGTGG
AAATGTCAGTTAGGACTTCCCTTATACTTATGGGCTGCCAAGACCCTGGAGGATCTCATCTACAAGCTGT
GTCCCCATGTGTTGAGATGTCACCAGTTCCTGAAGCAACCCTCTCCTGGGAAGCCCAGCTTGGGCCTGCA
GCTCCTGGCTCCCTCCTGGGATGTTCTGATTCCACCTGGCAGCCCCATGCGGCATGCGTACAGCGAGATC
CTGGAAGAACCACACAACTGCCCCCTTCAGAAATACACTCCACTGCAGACTCCCTACTCTTTCCCTACTA
TGCTCGCCCTGGCAGAGGAAGGGCAGCACAGGGCTTGGGCTACTTTTGACCCAAAGGCCATGCTGCCTCT
CCCAGAAGCCAGTCACCTGACCAGCTGCCAACTGAATCGCCACCTAGCCTGGTCCTGGGTCTGTCTGCCA
TCCTGTGTGTTCCAGCCAGCCCAGGTTTTACTTGGGGTCCTGGTGGCTTCATCTCGTAAAGGTTGTCTGG
AGCTTCGGGATCTAAGAGGTTCACTGCCTTGTATCCCCTTGACTGAGAGTTCACAGCCCCTCATTGACCC
GAATCTCGTAGGCTGCCTGGTGCGGGTAGAGAAGTTCCAGTTGGTAGTAGAGAGAGAAGTCAGGAGCAGC
TTCCCTTCCTGGGAGGAGATGGGCATGGCACGCTTCATCCAGAAGAAGCAGGCCAGAGTCTACGTCCAGT
TCTACCTGGCTGATGCCCTGATCCTGCCCGTGCCCAGACCCACCTTTGGTTCAGAACCATCTCAGACAGC
CTCCTCCTGCCCAGAGGGACCCCACCTGGGACAGAGCCGGCTGTTCTTACTGTCCCACAAGGAGGCCCTC
ATGAAACGTAATTTTTGTCTCCTTCCGGGAGACAGTTCACAACCTGCAAAGCCCACCTTGAGTTTCCACG
TGTCAGGAACTTGGCTTTGTGGCACACAGAGGAAGGAAGGTTCTGGATGGAGCCCACCCGAGTCTCTGGC
CGTTGAGAGCAAGGATCAGAAGGTCTTCCTCATCTTTCTTGGCTCTTCAGTTCGATGGTTTCCGTTCTTG
TACCCAAACCAAGTCTACCGACTCGTGGCTTCCGGCCCCTCACAGACACCAGTGTTCGAGACAGAGGGCT
CAGCTGGCACCTCTCGACGTCCTCTGGAACTGGCTGACTGTGGATCTTGCCTCACTGTCCAAGAGGAGTG
GACCCTGGAGCTTGGGAGCTCTCAGGACATACCTAATGTACTGGAGGTCCCTAGAACACTGCCCGAGTCC
TCACTGGCCCAGCTGCTCGGTGACAACTCCCCCGATTCCTTGGTATCTTTCTCTGCTGAGATTTTGTCTC
GGATACTGTGTGAACCACCTCTGGCCCTTCGCCGGATGAAACCTGGGAATGCTGGGGCTATTAAAACAGG
TGTGAAGCTAACAGTAGCTCTAGAAATGGATGACTGTGAATATCCCCCTCACCTGGACATATATATCGAA
GATCCACAGTTGCCTCCCCAAATAGGACTCCTTCCAGGAGCCCGAGTCCACTTTAGCCAGCTGGAGAAAA
GGATTTCCAGATCCAACATTGTTTACTGTTGTTTCCGGTCATCCACTTCTGTGCAGGTCCTGAGCTTCCC
CCCAGAGACCAAAGCCAGTGCTCCTCTGCCCCACATCTATCTGGCTGAACTTCTCCAAGGTGACCGGCCT
CCCTTCCAGGCCACTACCTCTTGCCATATTGTGTATGTCCTGAGCCTTCAGATCCTGTGGGTGTGTGCTC
ATTGCACCAGCATCTGCCCCCAGGGTAAGTGTAGTCGCCGGGACCCCAGTTGTCCATCTCAGAGGGCTGT
AAGCCAGGCCAACATCAGGCTCCTGGTAGAAGATGGCACCGCAGAAGCGACGGTGATCTGTAGGAATCAT
CTTGTGGCAAGGGCACTAGGGCTGAGTCCTAGTGAGTGGTCCTCCATCCTCGAGCATGCCAGGGGGCCGG
GGAGAGTGGCCTTGCAGTTTACAGGGCTAGGAGGCCAAACAGAGTCTGCAAGCAAAACCCATGAGCCCTT
GACCCTGCTCCTCCGGACACTTTGTACCAGCCCCTTTGTCCTCCGACCCGTTAAGCTTTCTTTTGCACTT
GAAAGGAGACCCACTGATATTTCCCCACGAGAACCGTCCCGGCTGCAGCAGTTCCAGTGTGGAGAGCTCC
CTCTGCTGACCAGAGTGAATCCCAGGCTCCGACTGGTCTGCCTTTCTCTGCAGGAGCCGGAGCTCCCCAA
CCCTCCACAAGCATCTGCTGCTTCGTCT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR211335 representing BC060629
Red=Cloning site Green=Tags(s)

MTLGELAQAGSQVSIIVQIPAQMVWHRVLRPGRAYVLTKLQVTKTRIHLSCIWTTIPSSTLKPLRPGYVQ
ELELDLEFSKADLKPPPQPTSSKDSRGQEGLVRASKVLHYLGTVTAVLHESAGLYILDGQLILCLAYQKI
HGLRRVIRPGVCLELRDVHLLQAVGGATTKPVLALCLHGTVRLQGFSCLKPLTLPSSKVYGASLYEQLVW
KCQLGLPLYLWAAKTLEDLIYKLCPHVLRCHQFLKQPSPGKPSLGLQLLAPSWDVLIPPGSPMRHAYSEI
LEEPHNCPLQKYTPLQTPYSFPTMLALAEEGQHRAWATFDPKAMLPLPEASHLTSCQLNRHLAWSWVCLP
SCVFQPAQVLLGVLVASSRKGCLELRDLRGSLPCIPLTESSQPLIDPNLVGCLVRVEKFQLVVEREVRSS
FPSWEEMGMARFIQKKQARVYVQFYLADALILPVPRPTFGSEPSQTASSCPEGPHLGQSRLFLLSHKEAL
MKRNFCLLPGDSSQPAKPTLSFHVSGTWLCGTQRKEGSGWSPPESLAVESKDQKVFLIFLGSSVRWFPFL
YPNQVYRLVASGPSQTPVFETEGSAGTSRRPLELADCGSCLTVQEEWTLELGSSQDIPNVLEVPRTLPES
SLAQLLGDNSPDSLVSFSAEILSRILCEPPLALRRMKPGNAGAIKTGVKLTVALEMDDCEYPPHLDIYIE
DPQLPPQIGLLPGARVHFSQLEKRISRSNIVYCCFRSSTSVQVLSFPPETKASAPLPHIYLAELLQGDRP
PFQATTSCHIVYVLSLQILWVCAHCTSICPQGKCSRRDPSCPSQRAVSQANIRLLVEDGTAEATVICRNH
LVARALGLSPSEWSSILEHARGPGRVALQFTGLGGQTESASKTHEPLTLLLRTLCTSPFVLRPVKLSFAL
ERRPTDISPREPSRLQQFQCGELPLLTRVNPRLRLVCLSLQEPELPNPPQASAASS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC060629
ORF Size 2898 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC060629.1
RefSeq Size 3247 bp
RefSeq ORF 2900 bp
Locus ID 68964
MW 106.6 kDa
Gene Summary Component of the CST complex proposed to act as a specialized replication factor promoting DNA replication under conditions of replication stress or natural replication barriers such as the telomere duplex. The CST complex binds single-stranded DNA with high affinity in a sequence-independent manner, while isolated subunits bind DNA with low affinity by themselves. Initially the CST complex has been proposed to protect telomeres from DNA degradation (PubMed:19854130). However, the CST complex has been shown to be involved in several aspects of telomere replication. The CST complex inhibits telomerase and is involved in telomere length homeostasis; it is proposed to bind to newly telomerase-synthesized 3' overhangs and to terminate telomerase action implicating the association with the ACD:POT1 complex thus interfering with its telomerase stimulation activity. The CST complex is also proposed to be involved in fill-in synthesis of the telomeric C-strand probably implicating recruitment and activation of DNA polymerase alpha. The CST complex facilitates recovery from many forms of exogenous DNA damage; seems to be involved in the re-initiation of DNA replication at repaired forks and/or dormant origins. Involved in telomere maintenance. Involved in genome stability (By similarity). May be in involved in telomeric C-strand fill-in during late S/G2 phase (PubMed:22748632).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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