Tcerg1 (BC039185) Mouse Tagged ORF Clone

CAT#: MR211555

  • TrueORF®

Tcerg1 (Myc-DDK-tagged) - Mouse transcription elongation regulator 1 (CA150) (cDNA clone MGC:28934 IMAGE:3982736)

ORF Plasmid: DDK tGFP



  "BC039185" in other vectors (4)

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Mouse Protein / Lysate Request

CNY 10,550.00


货期*
5周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Synonyms CA150b, p144, ca150, AI428505
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR211555 representing BC039185
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTCAACAGCAGGCCTTGAGGTTCCGAGGCCCAGCTCCCCCACCAAATGCCGTGATGCGAGGCCCGC
CACCTCTCATGATACCACCTCCTCCTTTCGGCATGATGAGAGGCCCTCCTCCACCACCCAGGCCCCCCTT
TGGACGGCCTCCTTTTGATCCTAATATGCCACCAATGCCTCCTCCAGGAGGAATACCTCCACCTATGGGC
CCACCTCACCTACAGAGACCACCTTTCATGCCTCCACCCATGGGAGCTATGCCTCCTCCTCCGGGTATGA
TGTTCCCACCAGGAATGCCTCCGGGGACTGCTCCTGGTGCTCCAGCACTGCCTCCCACTGAGGAGATATG
GGTAGAAAATAAAACTCCAGATGGGAAGGTTTATTACTACAATGCTCGGACTCGTGAATCTGCATGGACC
AAGCCAGATGGAGTTAAGGTTATTCAGCAATCAGAACTGACGCCCATGCTTGCAGCCCAAGCACAGGTTC
AGGCCCAGGCCCAGGCCCAAGCACAGGCTCAGGCCCAGGCCCAGGCACAAGCCCAGGCTCAGGCCCAGGC
CCAGGCCCAGGCTCAGGCACAAGCCCAGGCTCAGGCCCAGGCCCAGGCCCAGGCTCAGGCTCAGGCTCAA
GCCCAAGCGCAAGCACAAGCCCAGGCCCAAGCCCAGGCCCAGGCTCAGGCCCAGGCACAAGTCCAAGCAC
AAGCAGTAGGCGCTCCCACCCCTACCACCAGTAGCCCAGCACCTGCAGTGTCCACGTCAACACCGACATC
TACCCCATCCTCCACCACAGCTACCACGACAACTGCTACTTCAGTTGCACAGACAGTATCAACACCCACA
ACACAAGACCAGACCCCAAGTTCTGCTGTTTCAGTTGCCACACCTACAGTTAGTGTTTCAGCTCCTGCTC
CTACAGCCACACCTGTGCAAACCGTACCCCAGCCGCACCCACAGACGTTACCTCCTGCTGTTCCTCACTC
GGTACCTCAGCCGGCAGCAGCAATACCTGCTTTCCCACCAGTAATGGTGCCTCCGTTCCGTGTGCCCCTG
CCTGGCATGCCAATCCCACTTCCAGGTGTACTACCAGGAATGGCCCCTCCTATAGTACCCATGATCCATC
CCCAGGTTGCTATTGCAGCTTCACCTGCTACCCTAGCTGGGGCAACAGCAGTTTCTGAGTGGACTGAATA
TAAAACAGCAGATGGGAAGACGTACTATTATAATAATAGAACACTAGAATCCACGTGGGAGAAGCCCCAA
GAACTGAAGGAGAAAGAAAAATTAGATGAGAAGATTAAAGAACCAATTAAGGAGGCCTCTGAAGAGCCAT
TGCCAATGGAGACTGAGGAGGAAGATCCTAAGGAAGAGCCTGTGAAGGAGATAAAGGAGGAGCCCAAAGA
AGAAGAGATGACTGAAGAAGAAAAGGCTGCCCAGAAGGCAAAGCCAGTAGCTACTACTCCTATTCCTGGT
ACTCCATGGTGTGTTGTTTGGACTGGTGATGAGAGGGTCTTCTTTTACAATCCCACCACCCGCCTTTCTA
TGTGGGACCGGCCTGATGATTTGATTGGAAGGGCGGATGTTGACAAAATTATTCAAGAGCCCCCTCATAA
GAAAGGATTGGAAGACATGAAGAAGCTAAGGCACCCGGCTCCGACAATGCTGTCCATCCAAAAGTGGCAG
TTCTCAATGAGCGCAATTAAAGAAGAACAAGAGTTAATGGAAGAAATGAATGAAGATGAGCCTATTAAAG
CAAAAAAGCGGAAGAGAGATGATAATAAAGACATTGATTCAGAGAAAGAAGCTGCCATGGAAGCTGAAAT
TAAAGCTGCCCGAGAAAGGGCCATTGTCCCTCTGGAGGCTCGAATGAAGCAGTTCAAGGACATGCTGCTA
GAGAGAGGGGTGTCTGCTTTTTCAACATGGGAGAAAGAGTTGCACAAGATAGTATTTGACCCTCGGTACT
TGCTTCTCAATCCTAAAGAAAGAAAACAGGTGTTTGATCAGTATGTCAAGACCAGAGCAGAGGAAGAACG
CAGGGAAAAGAAAAACAAAATAATGCAAGCCAAGGAAGATTTCAAAAAAATGATGGAAGAAGCAAAGTTT
AATCCAAGAGCTACTTTTAGTGAATTTGCTGCTAAACATGCTAAAGATTCAAGATTCAAAGCAATTGAAA
AGATGAAAGATAGAGAAGCATTGTTTAATGAGTTTGTGGCAGCTGCAAGGAAGAAAGAAAAAGAAGATTC
GAAGACGAGAGGGGAGAAGATTAAATCAGATTTCTTTGAACTGTTATCTAATCATCACTTGGATAGTCAA
TCTCGATGGAGCAAAGTAAAAGACAAAGTAGAAAGTGACCCCCGATATAAAGCGGTGGATAGTTCATCAA
TGAGAGAAGACCTTTTCAAACAGTACATCGAGAAAATAGCCAAGAATTTAGACTCAGAAAAAGAAAAAGA
GCTGGAGAGGCAAGCCCGCATTGAGGCAAGCCTTCGAGAACGGGAAAGAGAAGTTCAGAAGGCACGCTCT
GAGCAGACGAAAGAGATCGATCGGGAGAGAGAGCAGCACAAACGGGAGGAGGCTATCCAGAACTTCAAGG
CTCTTCTGTCTGACATGGTACGTTCTTCAGATGTGTCCTGGTCTGATACTAGGAGGACCCTCCGGAAAGA
CCACCGCTGGGAATCTGGGTCCTTATTGGAAAGAGAGGAGAAAGAGAAACTTTTTAATGAACATATTGAA
GCGCTTACCAAAAAGAAAAGGGAGCACTTTAGGCAGCTTCTGGACGAAACCTCTGCGATTACCTTAACAT
CCACGTGGAAAGAAGTGAAAAAAATAATTAAGGAAGATCCTCGGTGCATTAAGTTCTCCTCCAGTGACAG
GAAAAAACAAAGGGAGTTTGAAGAATACATCAGAGACAAATACATCACTGCCAAAGCTGACTTCAGGACA
CTTTTGAAAGAGACCAAATTCATTACATACAGATCCAAAAAGTTAATCCAAGAATCTGATCAGCACCTGA
AAGATGTAGAAAAGATTTTGCAGAATGACAAACGGTATCTAGTACTGGACTGTGTCCCCGAGGAGAGGAG
GAAACTAATTGTGGCCTATGTTGATGACCTGGATCGCCGAGGTCCACCCCCACCTCCCACAGCATCAGAG
CCCACTAGACGATCAACAAAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR211555 representing BC039185
Red=Cloning site Green=Tags(s)

MAQQQALRFRGPAPPPNAVMRGPPPLMIPPPPFGMMRGPPPPPRPPFGRPPFDPNMPPMPPPGGIPPPMG
PPHLQRPPFMPPPMGAMPPPPGMMFPPGMPPGTAPGAPALPPTEEIWVENKTPDGKVYYYNARTRESAWT
KPDGVKVIQQSELTPMLAAQAQVQAQAQAQAQAQAQAQAQAQAQAQAQAQAQAQAQAQAQAQAQAQAQAQ
AQAQAQAQAQAQAQAQAQAQVQAQAVGAPTPTTSSPAPAVSTSTPTSTPSSTTATTTTATSVAQTVSTPT
TQDQTPSSAVSVATPTVSVSAPAPTATPVQTVPQPHPQTLPPAVPHSVPQPAAAIPAFPPVMVPPFRVPL
PGMPIPLPGVLPGMAPPIVPMIHPQVAIAASPATLAGATAVSEWTEYKTADGKTYYYNNRTLESTWEKPQ
ELKEKEKLDEKIKEPIKEASEEPLPMETEEEDPKEEPVKEIKEEPKEEEMTEEEKAAQKAKPVATTPIPG
TPWCVVWTGDERVFFYNPTTRLSMWDRPDDLIGRADVDKIIQEPPHKKGLEDMKKLRHPAPTMLSIQKWQ
FSMSAIKEEQELMEEMNEDEPIKAKKRKRDDNKDIDSEKEAAMEAEIKAARERAIVPLEARMKQFKDMLL
ERGVSAFSTWEKELHKIVFDPRYLLLNPKERKQVFDQYVKTRAEEERREKKNKIMQAKEDFKKMMEEAKF
NPRATFSEFAAKHAKDSRFKAIEKMKDREALFNEFVAAARKKEKEDSKTRGEKIKSDFFELLSNHHLDSQ
SRWSKVKDKVESDPRYKAVDSSSMREDLFKQYIEKIAKNLDSEKEKELERQARIEASLREREREVQKARS
EQTKEIDREREQHKREEAIQNFKALLSDMVRSSDVSWSDTRRTLRKDHRWESGSLLEREEKEKLFNEHIE
ALTKKKREHFRQLLDETSAITLTSTWKEVKKIIKEDPRCIKFSSSDRKKQREFEEYIRDKYITAKADFRT
LLKETKFITYRSKKLIQESDQHLKDVEKILQNDKRYLVLDCVPEERRKLIVAYVDDLDRRGPPPPPTASE
PTRRSTK

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC039185
ORF Size 3171 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC039185.1
RefSeq Size 4090 bp
RefSeq ORF 3173 bp
Locus ID 56070
MW 149.9 kDa
Gene Summary Transcription factor that binds RNA polymerase II and inhibits the elongation of transcripts from target promoters. Regulates transcription elongation in a TATA box-dependent manner (By similarity).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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