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Gemin4 (BC031425) Mouse Tagged ORF Clone
CAT＃: MR211560
- TrueORF®
Gemin4 (Myc-DDK-tagged) - Mouse gem (nuclear organelle) associated protein 4 (cDNA clone MGC:28146 IMAGE:3982927)
ORF Plasmid: tGFP
"BC031425" in other vectors (3)
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CNY 7,368.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | MGC28146, 4932415L08Rik |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR211560 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACCTAGGACCCTTGAATATCTGTGAGGAGATGACTATTCTGCATGGGGGCTTCTTGATGGCTGAGC AGCTGTTCCACCCCAAAGCCCTGGTACAGTTGACCAAGTCTGATTGGGAACACGTGGGACAGCCCATTGT GGAGGCCTTGAAGGAGATCTCCTCAGCCACAGCACATTCCCAGCCCTTTGCCTGGAAGAAGAAAGCACTG ATCATCATCTGGACCAAGGTTCTTCAGCCCTCCCCTGTCAGCCCTTCTGACACTGATACTCGGTGGCAGG AAGATGTATTCTTCTCCGTGGGCAGCATGATCCCTACCATCAACCACACAGTCCTTTTTGAGCTGCTCAA GTCTCTGGAAGCTTCGGGACTCTTCATTCAGCTGCTAATGGCCTTGCCTACCACCATTGGCCGCTCAGAA CTACAGAGCTTCCTGGAGCACATGACTGTTGACACATCTTCCAAGGATGTGGCCTTCTTCTTAGATGTCT GGTGGGAAATGATGAAGCACAAGGGCGACCAGCAGGACCCCTTGCTTTCCCAGTTCAGGACGATGGCCCA TAAATACTTGCCATCCTCAGATGAGTTTTCCCATCCTCCAAAGAGGTTTAAGTCTGACCCAGATGTGGGT CCCACCATGCCTCTTTTGGCCATGTTGCTTACCGGGCTGAAGCAGATCCAAAAGAGAATCCTGTCCCTTG AAATGAAGTGCTGTGCCTTAGCTAACTTGGCTGACATGCTGAGTGTGTTTGCACTTCTGGAGGATGACAC CCAGGAGGTGTCGGCCACTGTGTACCTAGACAAACTAGCTACAGTGATCTCTGTGTGGAACTCGGACACC CAGAACCCATACCATCGACAGGCCCTGGCAGACAAGGTAAAGGAGGCAGAGCGAGATGTGAGTCTCACCT CACTGGCCAAACTCCCCAGTGAGACTGTTTTCATGGGGTTTGAGCTTATGTACAACCTGCTGCAAGAGTG GGGTGCAGAACTGCAGGACATGCTCAACAGCAGTCAGGGAACAAATTATGACACCTACCGGCTGTGTGAC AGCCTGACTTCCTTCAGCCAAAACTTGGAGCTCTACCTGGATACTACCAACATGCCCAAGGAGGAGAGGG AGGTGGTCTCAGAATTGGCTGAGTGTGTCAGGGACTTCCTGAGGAAAACCAGCAGAATGCTGAATGACAA GGGCCTTGAGGACATCACTGCCTCCATTGCCATGGCCATCATTGAGCAGAAGATGGACCGGCACATGGAA ATGTGCTACATTTTTGCTTCTGAGAAGAAGTGGGCCTTTTCAGACGAGTGGGTTGCCTGCCTGGTTAACA ACAGGTCTCTCTTCAGAGAACCAGACTTGGTCTTGAGTCTGCTAGAAACAGTGATGGAAGTCATCAGCTC TGATCGGGTTATCCCCCAGCCTCAGATCAAGCAGGTAATCTGCTTGACCCTGGAATGCTATGAAGACCTC TCTCTTCCAGACAAAAATAAAGTTCTTTCTGGTGTCCTGCATTATTTGGGGCCAAAGGGTCTCTCAGACA GGTTGTCTGATTACTTGGAGGGTTTTCAGGAAGACCTCAACACGACGTTTAACCAGCTCGCACAGAGTAC CTCTGAACAGGGCCTGGCTAAAGCTGTGGCCTCCGTGGCCCACCTGGTGATCCTGAACCCAGAAGTTGTG GTGAAGAAGATGTGTAGCCTAGCTGTGACCAATCTTGGCACACATAGGTTCTTGGCTCAGATTCTCACTG CCTTCCCTGCTCTGAAGTTCACGGAAGAACAAGGGGCAAATCCATGTACCAGTTTCATGGTGTCTTGCCT CAAAGAAACTGTCTGGACCAAGTTCTCCACACCCAAAGAAGAGAAGCAGTTTCTGGAGCTGGTAAGCTGC CTGACGAATCCTGTGAAGCCACAGGGGATTCCAGTGGCTGCTCTTCTGGAGCCAGATGAGGTGCTCAAGG AGTTTGTCCTGCCTTTCTTGATGTTGGACATGGAGGAGGTGGGCCTGAGTCTGAAGCTCTTCATCCAGAC CCTCGAAGCCAATGCAGGCCTGGAAGAGTATTGGCTGCAGACCTGCTCCCCATTCCCTCTGCTCTTCAGC CTGTGCCAGCTCCTGGACAGCTTCAGCAAGTTCTGGCAGCTCCCCCGGGAGAAGCGCTACCTCACGCTGG ACAGCAAGGATCTTGTGACCCACATTCTGGAACTCCTCTGTGAGATCGTGCTAGCCAACACAGAAACCTT CTCCCCAGACACCTGGGCCAAGTCCCTGTCCTGGCTCCACCGCAAATTAGACCAGCTAGATTGGACTGTG GGCCTGAGACTAAAGAACTTCTTTGAAGGCCACTTCAAGTGTGAGGTGCCAGCTACCCTTTTTGATATCT GTAAGCTCTCTGAGGCCCAGTGGACCTCACAGGTCCACGAAGGGTATGGGCCTGGCACGGGCCTTCTCGC CTGGATGGAGTGCTGTTCAGTCTCCAGCAGTATCTCTGACCAGATGCTTTCCCTTCTGGTGATGGATGTG GGCAATCCTGAGGAGGTCAGACTGTTTAGCAAGGGCTTTCTGGTGGCCCTGGTACAAGTCATGCCTTGGT GTAGCCCCCAGGAATGGCAGTACCTTCAGCAGCTGACCAGGAAGCTATTGGAGAAACAGCTTCTTCATGT CCCTTACAGTCTAGAGTATATTCAGTTTGTTCCCCTGCTCAACCTGAAGCCTTTTGCCCAGGAACTTCAG CTCTCTGTTCTTTCTTTGAGAGTGTTCCAGTTTCTCTGCAGCCAGAGCTGTTGTAACTGGCTTCCTTTGG ACGGTTGGAGCCATGTGGTCAAACTCCTCTGTAACAGTCTGACCAGTGTCCTGGATGCAGTCAGGTTGAT GCAGTCGGTGGGTCCCTGGGCCCAAGGACAAAAAGGGGATCTTACTCAGGAAACCCTGTTTGTCTATACC CAGATGTTCTGTCATGTTCTGCATATCATGGCCATGCTCCCTCAGGAGGTCTGTGAACCCCTCTACGTGC TGGCCTTGGAAATCCTTACCTGCTACGAGACTCTAAGCAAAAGCAACCCTTCTGTCAGCTCCTTGCTCCA GAAGGTAGACGAGCAGCGCTTCTTAAAGTCTATCGCCAAGAACATTAGCCCTGAGGAGCGGCGCCAAACC CTGCTACAAAAGATCAACAACTTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR211560 protein sequence
Red=Cloning site Green=Tags(s) MDLGPLNICEEMTILHGGFLMAEQLFHPKALVQLTKSDWEHVGQPIVEALKEISSATAHSQPFAWKKKAL IIIWTKVLQPSPVSPSDTDTRWQEDVFFSVGSMIPTINHTVLFELLKSLEASGLFIQLLMALPTTIGRSE LQSFLEHMTVDTSSKDVAFFLDVWWEMMKHKGDQQDPLLSQFRTMAHKYLPSSDEFSHPPKRFKSDPDVG PTMPLLAMLLTGLKQIQKRILSLEMKCCALANLADMLSVFALLEDDTQEVSATVYLDKLATVISVWNSDT QNPYHRQALADKVKEAERDVSLTSLAKLPSETVFMGFELMYNLLQEWGAELQDMLNSSQGTNYDTYRLCD SLTSFSQNLELYLDTTNMPKEEREVVSELAECVRDFLRKTSRMLNDKGLEDITASIAMAIIEQKMDRHME MCYIFASEKKWAFSDEWVACLVNNRSLFREPDLVLSLLETVMEVISSDRVIPQPQIKQVICLTLECYEDL SLPDKNKVLSGVLHYLGPKGLSDRLSDYLEGFQEDLNTTFNQLAQSTSEQGLAKAVASVAHLVILNPEVV VKKMCSLAVTNLGTHRFLAQILTAFPALKFTEEQGANPCTSFMVSCLKETVWTKFSTPKEEKQFLELVSC LTNPVKPQGIPVAALLEPDEVLKEFVLPFLMLDMEEVGLSLKLFIQTLEANAGLEEYWLQTCSPFPLLFS LCQLLDSFSKFWQLPREKRYLTLDSKDLVTHILELLCEIVLANTETFSPDTWAKSLSWLHRKLDQLDWTV GLRLKNFFEGHFKCEVPATLFDICKLSEAQWTSQVHEGYGPGTGLLAWMECCSVSSSISDQMLSLLVMDV GNPEEVRLFSKGFLVALVQVMPWCSPQEWQYLQQLTRKLLEKQLLHVPYSLEYIQFVPLLNLKPFAQELQ LSVLSLRVFQFLCSQSCCNWLPLDGWSHVVKLLCNSLTSVLDAVRLMQSVGPWAQGQKGDLTQETLFVYT QMFCHVLHIMAMLPQEVCEPLYVLALEILTCYETLSKSNPSVSSLLQKVDEQRFLKSIAKNISPEERRQT LLQKINNF TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC031425 |
| ORF Size | 3174 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC031425, AAH31425 |
| RefSeq Size | 3424 bp |
| RefSeq ORF | 3176 bp |
| Locus ID | 276919 |
| MW | 120.2 kDa |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG211560 | Gemin4 (tGFP-tagged) - Mouse gem (nuclear organelle) associated protein 4 (cDNA clone MGC:28146 IMAGE:3982927) |
CNY 6,560.00 |
|
| MR211560L3 | Lenti ORF clone of Gemin4 (Myc-DDK-tagged) - Mouse gem (nuclear organelle) associated protein 4 (cDNA clone MGC:28146 IMAGE:3982927) |
CNY 7,890.00 |
|
| MR211560L4 | Lenti ORF clone of Gemin4 (mGFP-tagged) - Mouse gem (nuclear organelle) associated protein 4 (cDNA clone MGC:28146 IMAGE:3982927) |
CNY 7,890.00 |
