Gemin4 (BC031425) Mouse Tagged ORF Clone

CAT#: MR211560

  • TrueORF®

Gemin4 (Myc-DDK-tagged) - Mouse gem (nuclear organelle) associated protein 4 (cDNA clone MGC:28146 IMAGE:3982927)

ORF Plasmid: DDK tGFP



  "BC031425" in other vectors (3)

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Mouse Protein / Lysate Request

CNY 7,368.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Synonyms MGC28146, 4932415L08Rik
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR211560 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGACCTAGGACCCTTGAATATCTGTGAGGAGATGACTATTCTGCATGGGGGCTTCTTGATGGCTGAGC
AGCTGTTCCACCCCAAAGCCCTGGTACAGTTGACCAAGTCTGATTGGGAACACGTGGGACAGCCCATTGT
GGAGGCCTTGAAGGAGATCTCCTCAGCCACAGCACATTCCCAGCCCTTTGCCTGGAAGAAGAAAGCACTG
ATCATCATCTGGACCAAGGTTCTTCAGCCCTCCCCTGTCAGCCCTTCTGACACTGATACTCGGTGGCAGG
AAGATGTATTCTTCTCCGTGGGCAGCATGATCCCTACCATCAACCACACAGTCCTTTTTGAGCTGCTCAA
GTCTCTGGAAGCTTCGGGACTCTTCATTCAGCTGCTAATGGCCTTGCCTACCACCATTGGCCGCTCAGAA
CTACAGAGCTTCCTGGAGCACATGACTGTTGACACATCTTCCAAGGATGTGGCCTTCTTCTTAGATGTCT
GGTGGGAAATGATGAAGCACAAGGGCGACCAGCAGGACCCCTTGCTTTCCCAGTTCAGGACGATGGCCCA
TAAATACTTGCCATCCTCAGATGAGTTTTCCCATCCTCCAAAGAGGTTTAAGTCTGACCCAGATGTGGGT
CCCACCATGCCTCTTTTGGCCATGTTGCTTACCGGGCTGAAGCAGATCCAAAAGAGAATCCTGTCCCTTG
AAATGAAGTGCTGTGCCTTAGCTAACTTGGCTGACATGCTGAGTGTGTTTGCACTTCTGGAGGATGACAC
CCAGGAGGTGTCGGCCACTGTGTACCTAGACAAACTAGCTACAGTGATCTCTGTGTGGAACTCGGACACC
CAGAACCCATACCATCGACAGGCCCTGGCAGACAAGGTAAAGGAGGCAGAGCGAGATGTGAGTCTCACCT
CACTGGCCAAACTCCCCAGTGAGACTGTTTTCATGGGGTTTGAGCTTATGTACAACCTGCTGCAAGAGTG
GGGTGCAGAACTGCAGGACATGCTCAACAGCAGTCAGGGAACAAATTATGACACCTACCGGCTGTGTGAC
AGCCTGACTTCCTTCAGCCAAAACTTGGAGCTCTACCTGGATACTACCAACATGCCCAAGGAGGAGAGGG
AGGTGGTCTCAGAATTGGCTGAGTGTGTCAGGGACTTCCTGAGGAAAACCAGCAGAATGCTGAATGACAA
GGGCCTTGAGGACATCACTGCCTCCATTGCCATGGCCATCATTGAGCAGAAGATGGACCGGCACATGGAA
ATGTGCTACATTTTTGCTTCTGAGAAGAAGTGGGCCTTTTCAGACGAGTGGGTTGCCTGCCTGGTTAACA
ACAGGTCTCTCTTCAGAGAACCAGACTTGGTCTTGAGTCTGCTAGAAACAGTGATGGAAGTCATCAGCTC
TGATCGGGTTATCCCCCAGCCTCAGATCAAGCAGGTAATCTGCTTGACCCTGGAATGCTATGAAGACCTC
TCTCTTCCAGACAAAAATAAAGTTCTTTCTGGTGTCCTGCATTATTTGGGGCCAAAGGGTCTCTCAGACA
GGTTGTCTGATTACTTGGAGGGTTTTCAGGAAGACCTCAACACGACGTTTAACCAGCTCGCACAGAGTAC
CTCTGAACAGGGCCTGGCTAAAGCTGTGGCCTCCGTGGCCCACCTGGTGATCCTGAACCCAGAAGTTGTG
GTGAAGAAGATGTGTAGCCTAGCTGTGACCAATCTTGGCACACATAGGTTCTTGGCTCAGATTCTCACTG
CCTTCCCTGCTCTGAAGTTCACGGAAGAACAAGGGGCAAATCCATGTACCAGTTTCATGGTGTCTTGCCT
CAAAGAAACTGTCTGGACCAAGTTCTCCACACCCAAAGAAGAGAAGCAGTTTCTGGAGCTGGTAAGCTGC
CTGACGAATCCTGTGAAGCCACAGGGGATTCCAGTGGCTGCTCTTCTGGAGCCAGATGAGGTGCTCAAGG
AGTTTGTCCTGCCTTTCTTGATGTTGGACATGGAGGAGGTGGGCCTGAGTCTGAAGCTCTTCATCCAGAC
CCTCGAAGCCAATGCAGGCCTGGAAGAGTATTGGCTGCAGACCTGCTCCCCATTCCCTCTGCTCTTCAGC
CTGTGCCAGCTCCTGGACAGCTTCAGCAAGTTCTGGCAGCTCCCCCGGGAGAAGCGCTACCTCACGCTGG
ACAGCAAGGATCTTGTGACCCACATTCTGGAACTCCTCTGTGAGATCGTGCTAGCCAACACAGAAACCTT
CTCCCCAGACACCTGGGCCAAGTCCCTGTCCTGGCTCCACCGCAAATTAGACCAGCTAGATTGGACTGTG
GGCCTGAGACTAAAGAACTTCTTTGAAGGCCACTTCAAGTGTGAGGTGCCAGCTACCCTTTTTGATATCT
GTAAGCTCTCTGAGGCCCAGTGGACCTCACAGGTCCACGAAGGGTATGGGCCTGGCACGGGCCTTCTCGC
CTGGATGGAGTGCTGTTCAGTCTCCAGCAGTATCTCTGACCAGATGCTTTCCCTTCTGGTGATGGATGTG
GGCAATCCTGAGGAGGTCAGACTGTTTAGCAAGGGCTTTCTGGTGGCCCTGGTACAAGTCATGCCTTGGT
GTAGCCCCCAGGAATGGCAGTACCTTCAGCAGCTGACCAGGAAGCTATTGGAGAAACAGCTTCTTCATGT
CCCTTACAGTCTAGAGTATATTCAGTTTGTTCCCCTGCTCAACCTGAAGCCTTTTGCCCAGGAACTTCAG
CTCTCTGTTCTTTCTTTGAGAGTGTTCCAGTTTCTCTGCAGCCAGAGCTGTTGTAACTGGCTTCCTTTGG
ACGGTTGGAGCCATGTGGTCAAACTCCTCTGTAACAGTCTGACCAGTGTCCTGGATGCAGTCAGGTTGAT
GCAGTCGGTGGGTCCCTGGGCCCAAGGACAAAAAGGGGATCTTACTCAGGAAACCCTGTTTGTCTATACC
CAGATGTTCTGTCATGTTCTGCATATCATGGCCATGCTCCCTCAGGAGGTCTGTGAACCCCTCTACGTGC
TGGCCTTGGAAATCCTTACCTGCTACGAGACTCTAAGCAAAAGCAACCCTTCTGTCAGCTCCTTGCTCCA
GAAGGTAGACGAGCAGCGCTTCTTAAAGTCTATCGCCAAGAACATTAGCCCTGAGGAGCGGCGCCAAACC
CTGCTACAAAAGATCAACAACTTC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR211560 protein sequence
Red=Cloning site Green=Tags(s)

MDLGPLNICEEMTILHGGFLMAEQLFHPKALVQLTKSDWEHVGQPIVEALKEISSATAHSQPFAWKKKAL
IIIWTKVLQPSPVSPSDTDTRWQEDVFFSVGSMIPTINHTVLFELLKSLEASGLFIQLLMALPTTIGRSE
LQSFLEHMTVDTSSKDVAFFLDVWWEMMKHKGDQQDPLLSQFRTMAHKYLPSSDEFSHPPKRFKSDPDVG
PTMPLLAMLLTGLKQIQKRILSLEMKCCALANLADMLSVFALLEDDTQEVSATVYLDKLATVISVWNSDT
QNPYHRQALADKVKEAERDVSLTSLAKLPSETVFMGFELMYNLLQEWGAELQDMLNSSQGTNYDTYRLCD
SLTSFSQNLELYLDTTNMPKEEREVVSELAECVRDFLRKTSRMLNDKGLEDITASIAMAIIEQKMDRHME
MCYIFASEKKWAFSDEWVACLVNNRSLFREPDLVLSLLETVMEVISSDRVIPQPQIKQVICLTLECYEDL
SLPDKNKVLSGVLHYLGPKGLSDRLSDYLEGFQEDLNTTFNQLAQSTSEQGLAKAVASVAHLVILNPEVV
VKKMCSLAVTNLGTHRFLAQILTAFPALKFTEEQGANPCTSFMVSCLKETVWTKFSTPKEEKQFLELVSC
LTNPVKPQGIPVAALLEPDEVLKEFVLPFLMLDMEEVGLSLKLFIQTLEANAGLEEYWLQTCSPFPLLFS
LCQLLDSFSKFWQLPREKRYLTLDSKDLVTHILELLCEIVLANTETFSPDTWAKSLSWLHRKLDQLDWTV
GLRLKNFFEGHFKCEVPATLFDICKLSEAQWTSQVHEGYGPGTGLLAWMECCSVSSSISDQMLSLLVMDV
GNPEEVRLFSKGFLVALVQVMPWCSPQEWQYLQQLTRKLLEKQLLHVPYSLEYIQFVPLLNLKPFAQELQ
LSVLSLRVFQFLCSQSCCNWLPLDGWSHVVKLLCNSLTSVLDAVRLMQSVGPWAQGQKGDLTQETLFVYT
QMFCHVLHIMAMLPQEVCEPLYVLALEILTCYETLSKSNPSVSSLLQKVDEQRFLKSIAKNISPEERRQT
LLQKINNF

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC031425
ORF Size 3174 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC031425, AAH31425
RefSeq Size 3424 bp
RefSeq ORF 3176 bp
Locus ID 276919
MW 120.2 kDa
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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