Slc12a6 (BC062099) Mouse Tagged ORF Clone

CAT#: MR211642

  • TrueORF®

Slc12a6 (Myc-DDK-tagged) - Mouse solute carrier family 12, member 6 (cDNA clone MGC:69652 IMAGE:6844732)

ORF Plasmid: DDK tGFP



  "BC062099" in other vectors (4)

Need custom modification / cloning service?
Get a free quote

Mouse Protein / Lysate Request

CNY 11,020.00


货期*
5周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Synonyms 9530023I19Rik; gaxp; KCC3
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR211642 representing BC062099
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCATCCACCAGAAGCCACCACCAAGATGTCCTCAGTTCGGTTCATGGTGACACCAACTAAGATTGATG
ACATTCCAGGTTTGTCAGACACCAGCCCGGACCTCAGCTCTCGGTCTAGTTCTCGAGTAAGATTTAGCTC
CCGAGAAAGTGTGCCAGAAACAAGCCGTAGTGAGCCTATGAGCGAACTGTCTGGGGCTACTACTTCTCTG
GCAACTGTTGCCCTAGATCCTTCCAGTGACCGGACTTCTAATCCCCAGGATGTTACGGAGGATGACGGCC
ATAAAAAAGCCCGAAATGCTTATCTCAATAATTCCAACTATGAAGAAGGAGACGAATATTTTGATAAAAA
TTTGGCACTCTTTGAGGAAGAAATGGACACCAGACCAAAGGTGTCTTCTCTCCTCAACCGCATGGCCAAC
TATACAAATCTGACACAAGGAGCAAAGGAACATGAAGAGGCAGAGAACATCACTGAAGGGAAAAAGAAGC
CTACCAAGAGCCCCCAAATGGGTACTTTCATGGGTGTCTACCTCCCATGTCTACAGAACATCTTTGGAGT
GATCCTCTTCCTGCGTCTTACCTGGGTAGTGGGAACAGCTGGAATCCTTCAGGCCTTTGCAATTGTCCTC
ATCTGCTGCTGCTGTACAATGTTAACTGCCATCTCCATGAGCGCCATCGCCACTAACGGAGTGGTGCCAG
CTGGGGGCTCATACTTCATGATTTCCAGAGCCCTGGGCCCAGAGTTTGGCGGGGCTGTAGGCCTCTGCTT
TTATCTTGGCACCACATTTGCAGCAGCCATGTATATTCTTGGTGCCATTGAAATCTTTCTGGTATACATT
GTCCCCCGAGCTGCCATCTTTCGGAGTGACGATGCACTCAAGGAGTCAGCAGCTATGCTGAACAACATGC
GCGTCTATGGTACAGCCTTCTTGGTCCTCATGGTCTTGGTGGTATTCATCGGCGTACGCTATGTGAATAA
GTTTGCCTCACTCTTCCTGGCCTGTGTAATTGTGTCGATCTTGGCTATCTATGCTGGTGCCATCAAGTCT
TCCTTTGCTCCACCACACTTCCCGGTCTGTATGCTGGGCAACCGTACCCTGTCATCAAGACACCTTGACA
TTTGCTCTAAGACCAAGGAGGTTGACAACATGACAGTACCATCAAAGTTATGGGGATTCTTCTGCAACTC
GAGTCAGTTCTTTAATGCCACCTGTGATGAGTACTTTGTTCACAATAACGTCATCTCAATCCAAGGCATT
CCAGGGTTGGCTAGTGGTATCATTACTGAAAATCTTTGGAGTAATTATTTACCAAAGGGTGAGATAATTG
AAAAGCCATCAGCCAAGTCATCTGATGTCTTAGGCAACTTAAACCATGAATATGTTCTTGCTGATATCAC
CACCTCCTTCACTCTGCTGGTGGGGATCTTCTTTCCCTCGGTCACAGGTATCATGGCTGGGTCAAACAGA
TCTGGAGATCTGAAAGATGCCCAGAAGTCTATTCCCATTGGGACCATCCTTGCCATCCTGACCACATCCT
TTGTGTATTTAAGCAATGTTGTCCTTTTTGGTGCATGTATTGAAGGAGTCGTTCTCAGAGACAAATTTGG
GGATGCTGTAAAAGGGAATTTGGTTGTAGGCACCTTATCCTGGCCATCCCCGTGGGTGATCGTTATTGGC
TCCTTCTTTTCAACATGTGGGGCTGGGCTGCAGAGCCTCACAGGTGCGCCTCGGCTGCTGCAGGCTATCG
CCAAGGATAACATCATACCTTTCCTTAGGGTTTTTGGTCACAGCAAAGCTAATGGGGAACCTACCTGGGC
TTTACTTCTAACTGCTGCCATAGCAGAGCTGGGAATTCTCATCGCCTCCCTGGATCTCGTGGCCCCAATT
CTTTCCATGTTTTTTCTCATGTGTTACCTCTTTGTGAACTTGGCTTGTGCCTTGCAAACATTGCTGCGAA
CCCCCAACTGGAGGCCTCGATTCCGCTATTATCACTGGGCCCTCTCTTTCATGGGAATGAGTATCTGTCT
AGCTCTGATGTTCATTTCTTCTTGGTATTATGCCATTGTAGCTATGGTAATAGCTGGCATGATCTACAAG
TACATTGAATATCAAGGGGCTGAGAAAGAATGGGGGGATGGTATCCGTGGGCTGTCGCTCAGTGCAGCCC
GCTTCGCTTTGCTCCGTCTAGAGGAAGGACCTCCTCACACTAAAAACTGGAGGCCTCAGCTGCTCGTCCT
ACTGAAGCTGGATGAAGATTTACACGTCAAGCACCCTCGCCTCCTCACCTTTGCCTCCCAGCTCAAGGCA
GGAAAGGGACTCACGATTGTGGGCTCTGTCATCGTGGGGAACTTCTTGGAGAACTATGGTGACGCGCTCG
CGGCAGAGCAGACCATTAAGCACCTAATGGAGGCAGAAAAGGTAAAAGGATTCTGCCAATTGGTGGTGGC
TGCCAAGCTGAAAGAGGGCATTTCACACCTCATCCAGTCCTGTGGCCTCGGAGGCATGAAACACAACACA
GTGGTGATGGGCTGGCCCAATGGCTGGCGTCAGAGTGAAGATGCTCGCGCTTGGAAGACTTTCATTGGCA
CAGTACGAGTGACAACTGCTGCCCATCTAGCCCTGCTGGTGGCTAAAAATGTCTCCTTCTTTCCCAGCAA
TGTGGAGCAGTTTTCTGAGGGCAACATTGATGTGTGGTGGATTGTGCATGATGGGGGCATGCTCATGCTA
TTACCGTTCCTGCTGAAACAGCACAAGGTTTGGCGGAAATGCAGCATACGGATCTTCACAGTAGCCCAAC
TAGAAGACAACAGTATCCAGATGAAGAAGGATCTGGCCACCTTTCTGTACCACCTGCGCATTGAGGCAGA
AGTGGAAGTGGTGGAGATGCACGACAGTGACATATCTGCCTATACATATGAGCGCACCCTGATGATGGAG
CAGAGGTCCCAGATGCTTCGGCATATGCGGCTGTCCAAAACAGAGCGAGACAGGGAGGCACAGCTGGTGA
AAGATCGAAACTCAATGCTACGCTTGACCAGCATTGGCTCTGATGAGGACGAAGAGACAGAAACGTACCA
GGAGAAGGTGCACATGACTTGGACCAAGGATAAATACATGGCATCCCGGGGGCAAAAGGTCAAGTCAATG
GAAGGATTCCAGGACCTACTTAATATGCGTCCGGACCAGTCCAACGTGAGACGGATGCATACAGCAGTGA
AGCTCAATGAAGTTATAGTCAACAAGTCTCATGAAGCAAAGCTGGTTTTGTTGAATATGCCAGGACCACC
CCGGAACCCTGAAGGTGATGAAAACTGC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR211642 representing BC062099
Red=Cloning site Green=Tags(s)

MHPPEATTKMSSVRFMVTPTKIDDIPGLSDTSPDLSSRSSSRVRFSSRESVPETSRSEPMSELSGATTSL
ATVALDPSSDRTSNPQDVTEDDGHKKARNAYLNNSNYEEGDEYFDKNLALFEEEMDTRPKVSSLLNRMAN
YTNLTQGAKEHEEAENITEGKKKPTKSPQMGTFMGVYLPCLQNIFGVILFLRLTWVVGTAGILQAFAIVL
ICCCCTMLTAISMSAIATNGVVPAGGSYFMISRALGPEFGGAVGLCFYLGTTFAAAMYILGAIEIFLVYI
VPRAAIFRSDDALKESAAMLNNMRVYGTAFLVLMVLVVFIGVRYVNKFASLFLACVIVSILAIYAGAIKS
SFAPPHFPVCMLGNRTLSSRHLDICSKTKEVDNMTVPSKLWGFFCNSSQFFNATCDEYFVHNNVISIQGI
PGLASGIITENLWSNYLPKGEIIEKPSAKSSDVLGNLNHEYVLADITTSFTLLVGIFFPSVTGIMAGSNR
SGDLKDAQKSIPIGTILAILTTSFVYLSNVVLFGACIEGVVLRDKFGDAVKGNLVVGTLSWPSPWVIVIG
SFFSTCGAGLQSLTGAPRLLQAIAKDNIIPFLRVFGHSKANGEPTWALLLTAAIAELGILIASLDLVAPI
LSMFFLMCYLFVNLACALQTLLRTPNWRPRFRYYHWALSFMGMSICLALMFISSWYYAIVAMVIAGMIYK
YIEYQGAEKEWGDGIRGLSLSAARFALLRLEEGPPHTKNWRPQLLVLLKLDEDLHVKHPRLLTFASQLKA
GKGLTIVGSVIVGNFLENYGDALAAEQTIKHLMEAEKVKGFCQLVVAAKLKEGISHLIQSCGLGGMKHNT
VVMGWPNGWRQSEDARAWKTFIGTVRVTTAAHLALLVAKNVSFFPSNVEQFSEGNIDVWWIVHDGGMLML
LPFLLKQHKVWRKCSIRIFTVAQLEDNSIQMKKDLATFLYHLRIEAEVEVVEMHDSDISAYTYERTLMME
QRSQMLRHMRLSKTERDREAQLVKDRNSMLRLTSIGSDEDEETETYQEKVHMTWTKDKYMASRGQKVKSM
EGFQDLLNMRPDQSNVRRMHTAVKLNEVIVNKSHEAKLVLLNMPGPPRNPEGDENC

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC062099
ORF Size 3318 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC062099.1
RefSeq Size 4439 bp
RefSeq ORF 3320 bp
Locus ID 107723
MW 162.7 kDa
Gene Summary Mediates electroneutral potassium-chloride cotransport. May be activated by cell swelling. May contribute to cell volume homeostasis in single cells.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Other Versions

Customer Reviews 
Loading...