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Ubr2 (BC031403) Mouse Tagged ORF Clone
CAT＃: MR211646
- TrueORF®
Ubr2 (Myc-DDK-tagged) - Mouse ubiquitin protein ligase E3 component n-recognin 2 (cDNA clone MGC:36320 IMAGE:4935220)
ORF Plasmid: tGFP
"BC031403" in other vectors (4)
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CNY 11,020.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | MGC36320 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR211646 representing BC031403
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTGATAGAACATCCTCTTAGATGTCTTGTCTTATGTGCTCAAGTGCATGCTGGGATGTGGAGAAGAA ATGGCTTCTCTCTAGTAAATCAGATCTATTACTACCATAATGTGAAATGCAGGCGAGAGATGTTCGACAA GGACATAGTGATGCTTCAGACAGGTGTCTCCATGATGGACCCAAACCACTTCCTGATGATCATGCTCAGC CGCTTTGAACTCTATCAGCTCTTCAGCACGCCTGACTATGGGAAGAGATTCAGTTCTGAGGTTACCCATA AGGACGTCGTTCAGCAGAACAACACTCTGATCGAAGAGATGCTCTACCTCATCATCATGCTTGTGGGAGA AAGATTCAACCCTGGGGTTGGACAGGTGGCTGCCACAGATGAAATCAAGAGGGAGATTATCCATCAGTTG AGCATCAAGCCTATGGCTCACAGTGAGCTGGTGAAGTCTCTGCCTGAAGATGAGAACAAGGAGACCGGCA TGGAGAGCGTCATCGAGTCCGTTGCACATTTCAAGAAACCTGGGCTCACAGGGCGAGGCATGTATGAGCT GAAGCCAGAGTGTGCCAAAGAGTTCAACCTGTATTTTTATCATTTCTCCAGGGCAGAGCAGTCCAAGGCA GAGGAAGCTCAGCGGAAATTGAAAAGAGAAAATAAAGAAGATACAGCACTCCCTCCTCCGGCTTTGCCAC CGTTCTGCCCTTTGTTCGCGAGTCTGGTTAACATCTTGCAGTGTGACGTCATGCTGTACATCATGGGAAC GATCCTGCAGTGGGCTGTAGAGCATCACGGGTCTGCCTGGTCAGAGTCCATGCTACAGAGGGTGCTGCAT TTGATCGGGATGGCTCTCCAGGAAGAGAAGCACCACTTGGAGAACGCCGTGGAAGGGCACGTGCAGACCT TCACCTTCACACAGAAGATTTCAAAGCCTGGTGATGCACCACATAACTCCCCGAGCATCCTAGCTATGCT GGAGACCTTGCAGAACGCCCCCTCCCTGGAAGCCCACAAGGACATGATCAGGTGGTTGCTAAAGATGTTT AATGCAATTAAGAAGATAAGAGAGTGTTCATCCAGCAGCCCTGTGGCCGAGGCGGAGGGAACCATAATGG AGGAGAGCTCAAGAGACAAGGACAAAGCAGAGAGGAAAAGAAAAGCCGAGATCGCCAGACTGCGCCGGGA GAAGATCATGGCCCAGATGTCTGAGATGCAGCGGCACTTCATTGACGAAAACAAAGAGCTCTTCCAGCAG ACCCTAGAGCTGGACACCTCTGCCTCTGCCACTCTTGACAGCAGCCCTCCCGTTTCAGACGCAGCTCTTA CAGCACTGGGCCCAGCACAGACACAGGTCCCTGAACCGAGACAGTTTGTCACCTGTATATTATGTCAAGA GGAGCAAGAGGTGACTGTGGGAAGCAGGGCGATGGTCTTGGCAGCGTTTGTTCAGAGGTCAACGGTTCTG TCAAAAGACAGGACGAAAACCATCGCGGACCCAGAAAAATATGATCCATTATTCATGCACCCCGATCTGT CTTGTGGGACACACACTGGCAGCTGTGGGCACGTTATGCATGCCCATTGTTGGCAAAGGTATTTTGATTC CGTTCAAGCCAAGGAGCAGCGAAGGCAGCAGCGGCTGCGCTTGCACACTAGCTACGATGTAGAGAATGGC GAGTTCCTCTGCCCGCTCTGTGAGTGCCTGAGCAACACGGTGATCCCCCTGCTGCTTCCTCCCAGGAGCA TCCTCAGCAGGAGGTTAAATTTTTCAGACCAACCAGATCTGGCACAGTGGACGAGAGCAGTAACACAGCA GATAAAGGTGGTCCAGATGCTGAGGAGAAAGCACAATGCTGCTGACACGTCTTCTTCAGAGGACACAGAA GCCATGAATATAATACCGATCCCCGAAGGCTTCAGGCCTGATTTTTATCCTAGGAACCCATATTCTGATA GCATAAAAGAAATGTTAACGACATTTGGAACGGCTGCTTACAAGGTGGGACTGAAGGTTCATCCTAATGA AGGTGACCCCCGTGTGCCCATCCTGTGCTGGGGGACCTGTGCATACACCATCCAGAGCATAGAAAGAATT TTGAGTGATGAGGAGAAGCCTGTTTTTGGACCTCTGCCTTGTAGACTGGACGACTGTCTCAGGTCGTTAA CACGGTTTGCAGCAGCACATTGGACAGTGGCGTTACTTCCTGTGGTACAAGGACACTTCTGTAAACTCTT TGCATCCTTGGTGCCTAGTGACAGCTATGAAGACCTCCCGTGCATACTAGACATCGACATGTTTCACTTG CTGGTGGGCCTGGTGCTCGCTTTCCCAGCTCTGCAGTGTCAGGATTTTTCAGGAAGCAGCCTGGCCACTG GGGACCTGCACATCTTCCACTTGGTTACCATGGCACACATCGTACAGATCTTACTTACCTCATGTACAGA AGAGAATGGCATGGATCAAGAGAATCCCACTGGGGAAGAAGAACTGGCCATTCTCTCTTTGCACAAAACA CTTCACCAGTATACTGGAAGTGCCTTGAAAGAAGCCCCCTCCGGCTGGCACCTGTGGAGGAGCGTCCGGG CCGCCATCATGCCTTTCCTCAAGTGCTCTGCTTTGTTTTTCCACTATTTAAATGGAGTCCCGGCCCCTCC AGACCTTCAAGTTTCTGGAACAAGCCATTTTGAACACTTATGTAACTACCTTTCCCTACCAACCAACCTC ATTCACCTTTTTCAAGAAAACAGTGACATCATGAACTCCCTGATTGAAAGTTGGTGCCAGAACAGTGAAG TTAAACGGTATCTAAATGGCGAGAGAGGAGCGATAAGCTACCCCAGAGGAGCTAACAAACTGATAGACCT TCCAGAGGATTACAGCAGCCTCATTAACCAAGCATCCAATTTCTCGTGCCCCAAATCAGGTGGCGACAAG AGCAGAGCTCCTACTCTGTGCCTCGTGTGTGGGAGTCTCCTCTGCTCTCAGAGTTACTGCTGCCAAGCTG AGCTGGAGGGTGAGGACGTCGGAGCCTGCACAGCACACACCTACTCCTGCGGCTCCGGGGCCGGCATCTT CCTGAGAGTGCGGGAATGTCAGGTGCTATTTTTAGCTGGCAAAACCAAAGGATGTTTTTATTCTCCTCCT TACCTTGACGACTATGGAGAGACCGACCAGGGACTCAGACGAGGAAATCCTTTACATTTATGCCAAGAGC GGTTTCGAAAGATCCAGAAGCTCTGGCAGCAGCATAGTATCACAGAGGAGATCGGACACGCGCAGGAGGC TAACCAGACCCTGGTCGGAATTGACTGGCAGCATTTA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR211646 representing BC031403
Red=Cloning site Green=Tags(s) MLIEHPLRCLVLCAQVHAGMWRRNGFSLVNQIYYYHNVKCRREMFDKDIVMLQTGVSMMDPNHFLMIMLS RFELYQLFSTPDYGKRFSSEVTHKDVVQQNNTLIEEMLYLIIMLVGERFNPGVGQVAATDEIKREIIHQL SIKPMAHSELVKSLPEDENKETGMESVIESVAHFKKPGLTGRGMYELKPECAKEFNLYFYHFSRAEQSKA EEAQRKLKRENKEDTALPPPALPPFCPLFASLVNILQCDVMLYIMGTILQWAVEHHGSAWSESMLQRVLH LIGMALQEEKHHLENAVEGHVQTFTFTQKISKPGDAPHNSPSILAMLETLQNAPSLEAHKDMIRWLLKMF NAIKKIRECSSSSPVAEAEGTIMEESSRDKDKAERKRKAEIARLRREKIMAQMSEMQRHFIDENKELFQQ TLELDTSASATLDSSPPVSDAALTALGPAQTQVPEPRQFVTCILCQEEQEVTVGSRAMVLAAFVQRSTVL SKDRTKTIADPEKYDPLFMHPDLSCGTHTGSCGHVMHAHCWQRYFDSVQAKEQRRQQRLRLHTSYDVENG EFLCPLCECLSNTVIPLLLPPRSILSRRLNFSDQPDLAQWTRAVTQQIKVVQMLRRKHNAADTSSSEDTE AMNIIPIPEGFRPDFYPRNPYSDSIKEMLTTFGTAAYKVGLKVHPNEGDPRVPILCWGTCAYTIQSIERI LSDEEKPVFGPLPCRLDDCLRSLTRFAAAHWTVALLPVVQGHFCKLFASLVPSDSYEDLPCILDIDMFHL LVGLVLAFPALQCQDFSGSSLATGDLHIFHLVTMAHIVQILLTSCTEENGMDQENPTGEEELAILSLHKT LHQYTGSALKEAPSGWHLWRSVRAAIMPFLKCSALFFHYLNGVPAPPDLQVSGTSHFEHLCNYLSLPTNL IHLFQENSDIMNSLIESWCQNSEVKRYLNGERGAISYPRGANKLIDLPEDYSSLINQASNFSCPKSGGDK SRAPTLCLVCGSLLCSQSYCCQAELEGEDVGACTAHTYSCGSGAGIFLRVRECQVLFLAGKTKGCFYSPP YLDDYGETDQGLRRGNPLHLCQERFRKIQKLWQQHSITEEIGHAQEANQTLVGIDWQHL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC031403 |
| ORF Size | 3327 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC031403.1 |
| RefSeq Size | 3502 bp |
| RefSeq ORF | 3329 bp |
| Locus ID | 224826 |
| MW | 125.3 kDa |
| Gene Summary | E3 ubiquitin-protein ligase which is a component of the N-end rule pathway. Recognizes and binds to proteins bearing specific N-terminal residues that are destabilizing according to the N-end rule, leading to their ubiquitination and subsequent degradation. Plays a critical role in chromatin inactivation and chromosome-wide transcriptional silencing during meiosis via ubiquitination of histone H2A. Binds leucine and is a negative regulator of the leucine-mTOR signaling pathway, thereby controlling cell growth (By similarity). Required for spermatogenesis, promotes, with Tex19.1, SPO11-dependent recombination foci to accumulate and drive robust homologous chromosome synapsis (PubMed:28708824). Polyubiquitinates LINE-1 retrotransposon encoded, LIRE1, which induces degradation, inhibiting LINE-1 retranstoposon mobilization (PubMed:28806172).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC223125 | Ubr2 (untagged) - Mouse ubiquitin protein ligase E3 component n-recognin 2 (cDNA clone MGC:36320 IMAGE:4935220), (10ug) |
CNY 12,920.00 |
|
| MG211646 | Ubr2 (tGFP-tagged) - Mouse ubiquitin protein ligase E3 component n-recognin 2 (cDNA clone MGC:36320 IMAGE:4935220) |
CNY 12,160.00 |
|
| MR211646L3 | Lenti ORF clone of Ubr2 (Myc-DDK-tagged) - Mouse ubiquitin protein ligase E3 component n-recognin 2 (cDNA clone MGC:36320 IMAGE:4935220) |
CNY 12,920.00 |
|
| MR211646L4 | Lenti ORF clone of Ubr2 (mGFP-tagged) - Mouse ubiquitin protein ligase E3 component n-recognin 2 (cDNA clone MGC:36320 IMAGE:4935220) |
CNY 12,920.00 |
