Smc4 (BC061481) Mouse Tagged ORF Clone

CAT#: MR211813

  • TrueORF®

Smc4 (Myc-DDK-tagged) - Mouse structural maintenance of chromosomes 4 (cDNA clone MGC:70057 IMAGE:6512832)

ORF Plasmid: DDK tGFP



  "BC061481" in other vectors (3)

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Mouse Protein / Lysate Request

CNY 8,464.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Synonyms 2500002A22Rik; C79747; SMC-4; Smc4l1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR211813 representing BC061481
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCAGTGAAGCTGGAGCTCCTCGTCTCATGATAACTCATATTGTAAACCAAAACTTCAAGTCCTATG
CTGGAGAGAAAGTTCTGGGACCTTTCCATAAGCGCTTTTCCTGTATTATTGGACCAAATGGCAGTGGAAA
ATCCAATGTTATTGATTCCATGCTTTTTGTGTTTGGCTATCGAGCACAAAAAATAAGGTCTAAAAAGCTA
TCGGTACTGATACATAATTCTGATGAACACAAGGATATTCAGAGTTGTACTGTAGAAGTTCATTTTCAAA
AGATAATTGATAAGGAAGGAGATGATTACGAAGTCCTTCCTAACAGCAACTTCTATGTATCCAGAACCGC
CTACAGAGATAGCACTTCTGTCTACCACATAAGTGGGAAGAAGAAGACGTTTAAAGATGTTGGAAATCTC
CTTAGAAGCCATGGCATTGACTTAGACCATAATCGATTTTTAATCTTGCAGGGTGAAGTTGAACAGATTG
CTATGATGAAACCCAAAGGCCAGACTGAACATGATGAAGGTATGCTTGAGTATTTAGAAGATATAATTGG
CTGTGGACGGCTAAATGAACCTATTAAAGTCTTGTGTCGGAGAGTTGAAATATTAAATGAACACAGAGGA
GAAAAGTTAAATAGAGTGAAAATGGTAGAAAAGGAGAAGGATGCACTAGAGGGAGAGAAAAACATAGCCA
TTGAGTTCCTTACCCTGGAAAATGAAATGTTTAAGAAAAAGAATCACATCTGTCAATACTACATTTATGA
TCTGCAGAATAGAATTGCTGAAATAACGACTCAAAAGGAAAAAATTCATGAAGATACTAAAGAAATTACT
GAGAAGAGCAATGTGCTTTCCAATGAAATGAAAGCGAAGAATTCAGCTGTAAAGGATGTAGAAAAGAAGC
TCAATAAAGTTACAAAATTTATCGAACAGAATAAAGAAAAATTTACACAACTAGATTTGGAAGATGTTCA
AGTTAGGGAAAAATTAAAACATGCTACAAGTAAAGCCAAGAAACTGGAGAAACAACTTCAAAAAGATAAA
GAAAAGGTTGAAGAGCTGAAAAGTGTACCTGCCAAGAGTAAGACTGTCATAAATGAGACAACAACTAGAA
ACAATTCGCTTGAGAAAGAAAGAGAGAAAGAAGAAAAAAAATTAAAAGAGGTCATGGACAGCCTTAAGCA
GGAAACACAGGGGCTTCAGAAAGAAAAGGAGATTCAAGAAAAGGAACTTATGGGTTTTAACAAATCAGTA
AATGAAGCACGTTCAAAGATGGAAGTTGCTCAGTCAGAACTTGATATCTATCTCAGTCGACATAATACAG
CAGTGTCTCAATTAAGTAAGGCGAAGGAAGCCTTAATTACAGCTTCTGAGACTCTCAAGGAGAGGAAAGC
TGCAATCAAGGATATAAACACAAAGCTCCCTCAGACGCAACAGGAACTAAAGGAGAAAGAAAAAGAACTT
CAGAAACTGACACAAGAAGAAATAAACTTAAAAAGTTTGGTTCATGATCTTTTCCAAAAAGTTGAAGAAG
CAAAGAGTTCCTTAGCAATGAATCGAAGTAGGGGGAAAGTACTTGATGCAATAATTCAAGAAAAAAAATC
AGGCAGGATCCCAGGAATATATGGAAGATTGGGAGACTTAGGAGCTATTGATGAAAAATATGACATTGCT
ATTTCATCCTGTTGCCATGCATTGGACTACATTGTTGTTGATTCTATTGATACAGCCCAAGAATGTGTAA
ACTTCCTTAAAAAGCATAATATTGGGATTGCAACTTTCATAGGTTTGGATAAGATGACAGTGTGGGCCAA
AAAAATGAGCAAAATTCAAACTCCTGAAAATACTCCTCGGTTATTTGATCTAGTTAAAGTGAAAAATGAG
GAAATCCGCCAAGCCTTTTACTTTGCTTTACGAGATACCTTGGTGGCTAACAATTTGGATCAAGCTACAA
GAGTAGCGTACCAAAGAGACAGACGCTGGAGAGTAGTGACTCTGCAGGGACAGATCATAGAACAGTCAGG
TACAATGAGTGGCGGTGGAAGCAAGGTGATGAGAGGAAGGATGGGCTCTTCGGTTATCGATGAAATCTCT
GTAGAAGAGGTAAATAAGATGGAATCCCAGTTGGAAAGGCATTCTAAACAAGCAATGCAAATCCAAGAAC
AGAAAGTACAACATGAAGAAGCAGTAGTTAAGTTACGGCACAGTGAACGAGACATGAGAAATACACTGGA
AAAGTTCGCTGCAAGCATCCAGGGCTTATCAGAGCAAGAAGAGTATCTGTGTGTACAAATTAAAGAACTG
GAAGCTAACGTTCTTACTACGGCCCCTGACAGAAAACAACAGAAACTGCTGGAAGAAAATGTCAGTGTTT
TCAAAAAAGAATATGATGCTGTGGCTGAGAAAGCTGGTAAAGTTGAAGCTGAGATTAAACGGCTACACAA
TACCATCATAGATATCAACAACCGAAAACTCAAGGCCCAGCAAAACAAACTTGACACGATAAATAAGCAG
CTTGATGAATGTGCTTCTGCTATTACTAAGGCCCAAGTAGCAATCAAGACTGCTGACAGAAATCTTAAAA
AGGCACAGGATTCTGTTTGCCGCACAGAGAAAGAAATAAAAGACACTGAAAAAGAAATAAATGACTTAAA
GACAGAGCTAAAAAATATTGAAGACAAGGCAGAAGAGGTCATAAACAATACAAAAACTGCAGAGACATCC
TTACCAGAAATCCAGAAAGAACATCGTAATCTACTTCAGGAACTAAAAGTTATTCAAGAAAATGAACATG
CTCTTCAGAAAGATGCACTTAGTATTAAGTTGAAACTTGAACAGATAGATGGCCACATTTCTGAACATAA
CTCAAAAATAAAATACTGGCAAAAAGAGATTTCAAAAATAAAATTGCATCCTGTTGAAGATAATCCTGTT
GAGACAGTAGCAGTTCTAAGCCAGGAGGAGCTTGAGGCAATCAAGAATCCAGAGTCTATAACAAATGAAA
TTGCACTTTTGGAAGCTCAGTGTCGTGAAATGAAACCAAACCTTGGAGCCATCGCTGAGTATAAAAAAAA
GGAAGATTTATATTTGCAAAGAGTAGCCGAACTGGACAAAATTACTTCTGAAAGAGATAATTTTAGACAA
GCATATGAAGATCTTCGAAAACAAAGGCTGAATGAATTTATGGCTGGTTTTTACGTAATAACAAATAAAC
TAAAAGAAAACTACCAGATGCTCACATTGGGAGGAGATGCTGAACTGGAGCTTGTGGACAGTTTAGATCC
TTTTTCTGAAGGAATCATGTTCAGTGTTCGGCCACCTAAGAAAAGTTGGAAGAAGATCTTTAACCTCTCA
GGAGGCGAGAAAACCCTTAGTTCCTTGGCCTTAGTGTTTGCTCTTCATCACTACAAGCCCACTCCCCTCT
ACTTCATGGATGAGATTGATGCAGCTCTGGATTTTAAAAATGTGTCTATTGTTGCATTTTATATATATGA
GCAAACAAAAAATGCCCAGTTCATAATAATCTCTCTTCGAAATAATATGTTTGAGATTTCAGACAGACTT
ATTGGCATCTATAAAACATATAATAGTACAAAAAGTGTTGCAGTGAACCCAAAACAAATTGCGTCTAAAG
GACTCTGT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR211813 representing BC061481
Red=Cloning site Green=Tags(s)

MASEAGAPRLMITHIVNQNFKSYAGEKVLGPFHKRFSCIIGPNGSGKSNVIDSMLFVFGYRAQKIRSKKL
SVLIHNSDEHKDIQSCTVEVHFQKIIDKEGDDYEVLPNSNFYVSRTAYRDSTSVYHISGKKKTFKDVGNL
LRSHGIDLDHNRFLILQGEVEQIAMMKPKGQTEHDEGMLEYLEDIIGCGRLNEPIKVLCRRVEILNEHRG
EKLNRVKMVEKEKDALEGEKNIAIEFLTLENEMFKKKNHICQYYIYDLQNRIAEITTQKEKIHEDTKEIT
EKSNVLSNEMKAKNSAVKDVEKKLNKVTKFIEQNKEKFTQLDLEDVQVREKLKHATSKAKKLEKQLQKDK
EKVEELKSVPAKSKTVINETTTRNNSLEKEREKEEKKLKEVMDSLKQETQGLQKEKEIQEKELMGFNKSV
NEARSKMEVAQSELDIYLSRHNTAVSQLSKAKEALITASETLKERKAAIKDINTKLPQTQQELKEKEKEL
QKLTQEEINLKSLVHDLFQKVEEAKSSLAMNRSRGKVLDAIIQEKKSGRIPGIYGRLGDLGAIDEKYDIA
ISSCCHALDYIVVDSIDTAQECVNFLKKHNIGIATFIGLDKMTVWAKKMSKIQTPENTPRLFDLVKVKNE
EIRQAFYFALRDTLVANNLDQATRVAYQRDRRWRVVTLQGQIIEQSGTMSGGGSKVMRGRMGSSVIDEIS
VEEVNKMESQLERHSKQAMQIQEQKVQHEEAVVKLRHSERDMRNTLEKFAASIQGLSEQEEYLCVQIKEL
EANVLTTAPDRKQQKLLEENVSVFKKEYDAVAEKAGKVEAEIKRLHNTIIDINNRKLKAQQNKLDTINKQ
LDECASAITKAQVAIKTADRNLKKAQDSVCRTEKEIKDTEKEINDLKTELKNIEDKAEEVINNTKTAETS
LPEIQKEHRNLLQELKVIQENEHALQKDALSIKLKLEQIDGHISEHNSKIKYWQKEISKIKLHPVEDNPV
ETVAVLSQEELEAIKNPESITNEIALLEAQCREMKPNLGAIAEYKKKEDLYLQRVAELDKITSERDNFRQ
AYEDLRKQRLNEFMAGFYVITNKLKENYQMLTLGGDAELELVDSLDPFSEGIMFSVRPPKKSWKKIFNLS
GGEKTLSSLALVFALHHYKPTPLYFMDEIDAALDFKNVSIVAFYIYEQTKNAQFIIISLRNNMFEISDRL
IGIYKTYNSTKSVAVNPKQIASKGLC

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC061481
ORF Size 3648 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC061481.1
RefSeq Size 4097 bp
RefSeq ORF 3650 bp
Locus ID 70099
MW 150.2 kDa
Gene Summary Central component of the condensin complex, a complex required for conversion of interphase chromatin into mitotic-like condense chromosomes. The condensin complex probably introduces positive supercoils into relaxed DNA in the presence of type I topoisomerases and converts nicked DNA into positive knotted forms in the presence of type II topoisomerases (By similarity).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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