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Oplah (NM_153122) Mouse Tagged ORF Clone
CAT＃: MR211894
- TrueORF®
Oplah (Myc-DDK-tagged) - Mouse 5-oxoprolinase (ATP-hydrolysing) (Oplah)
ORF Plasmid: tGFP
"NM_153122" in other vectors (4)
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CNY 8,968.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 1700010G02Rik |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR211894 representing NM_153122
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCAGCCCAGAAGAGCGCTTCCATTTTGCCATCGACCGTGGTGGCACCTTTACAGATGTCTTTGCCC AGTGCCCAGGAGGGCATGTACGTGTCCTGAAGCTGCTCTCAGAGGATCCTGCCAACTATGCAGACGCACC CACAGAGGGCATCCGCCGAATTCTAGAGCAGGAGAGGGGTGTGCTGCTGCCCCGAGGCCGGCCGCTAGAC ACCAGTCACATCGCCAGCATCCGCATGGGTACCACCGTGGCCACCAATGCTCTGTTGGAACGGCAGGGAG AACGGGTGGCACTGCTGGTGACTCGCGGTTTCCGAGACCTGCTGCATATTGGCACTCAGGCCCGCCCAGA CCTCTTTGACCTGGCTGTGCCCATGCCAGAGGTACTGTATGAGGAGGTCGTGGAGGTGGACGAGCGAGTG CTGCTGTATCGTGGAGAACCCGGTGCCGGCTCTCCCGTCAAAGGCTGTACAGGGGACCTGCTAGAGATAC AGCAGCCTGTGGATCTGGCAGCCCTGCGTGGGAAGCTGGAGGGGCTCTTGACTCGGGGCATTCACAGCCT GGCAGTGGTACTCATGCATTCGTACACGTGGGCCCAGCACGAGCAGCAGGTGGGCACACTGGCCCGGGAG CTGGGCTTCACGCACGTCTCCTTGTCCTCGGAAGTCATGCCCATGGTGCGGATTGTTCCTCGGGGGCATA CGGCCTGTGCAGATGCTTACCTCACTCCCACCATCCAGCGCTATGTGCAGGGCTTCCGCCGAGGCTTCCA GGGCCAGCTAAAGAATGTGCAAGTCCTCTTCATGCGCTCCGATGGTGGCCTGGCACCCATGGATGCCTTC AGTGGCTCCCGGGCTGTGCTCTCTGGCCCTGCTGGTGGTGTGGTTGGCTACTCCACTACCACCTACCAGC TGGAAGGTGGTCAGCCTGTCATTGGCTTTGACATGGGAGGCACATCCACAGATGTGAGCCGCTATGCTGG AGAGTTTGAGCACGTCTTTGAGGCTAGCACGGCAGGTGTTACCCTCCAGGCCCCACAGCTGGACATCAAT ACGGTGGCAGCTGGCGGGGGTTCCCGCCTCTTCTTCAGGTCTGGCCTCTTTGTGGTTGGTCCAGAGTCAG CAGGTGCCCACCCAGGTCCTGCCTGCTACCGCAAAGGGGGTCCTGTGACAGTGACAGATGCTAATCTGGT TCTGGGTCGCCTGCTGCCTGCCTCCTTCCCCTGCATTTTTGGGCCAGGAGAAGACCAGCCATTGTCTCCC GAGGCTTCCCGAAAGGCTCTAGAGGCTGTGGCCATGGAGGTCAACAGTTTCTTGGCCAGTGGACCCTGCC CAGCTTCCCAGCTAAGTCTGGAGGAGGTGGCCATGGGGTTTGTGCGTGTTGCCAACGAAGCCATGTGCCG GCCTATCCGTGCCCTCACACAGGCACGAGGCCACGACCCCTCAGCCCATGTACTGGCTTGCTTTGGAGGA GCCGGTGGGCAACACGCTTGTGCCATTGCCCGGGCCCTGGGTATGGACACTGTGCATATTCACAGGCACA GCGGGCTGCTGTCAGCACTGGGACTGGCCTTGGCCGATGTGGTTCACGAAGCACAGGAGCCCTGTTCCCT GTCTTACACACCTGAAACCTTTGCACAACTGGACCAGAGACTGAGCCGCCTGGAGGAGCAGTGTGTGGAT GCCCTGCAGGCCCAGGGCTTCTCTAGGTCTCAGATCAGCACCGAGAGCTTCCTGCACCTTCGCTACCAAG GCACTGACTGTGCCCTAATGGTGTCTGCCAATCAGCATCCGGCCACAACCTGCTCACCCCGTGCTGGTGA CTTTGGAGCAGCGTTTGTCGAGAGGTACATGAGAGAGTTTGGCTTCATCATTCCTGAGCGCTCGGTGGTG GTAGATGATGTGCGTGTGAGGGGAACTGGCCGTAGTGGACTTCAGCTGGAGGAGACCTCCAAAATCCAGA GTGGACCTCCGCATGTGGAAAAGGTGACCCAGTGCTACTTTGAAGGGGGTTATCAGGAGACTCCCGTGTA CCTTTTAGGAGAACTAGGCTACGGGCATCAGCTTCAAGGGCCCTGCCTGATCATTGACAACAACAGCACC ATCCTTGTAGAACCAGGTTGCCAAGCGGAGGTGATTGAGACAGGGGACATCCGCATTTCTGTGGGAGCCG AGGCTCCCAGTATGATAGACACCAAGCTTGACCCCATCCAGTTGTCTATTTTCTCACACCGTTTCATGAG CATTGCTGAGCAGATGGGCCGCATCCTACAGCGCACAGCCATCTCTACCAACATCAAGGAACGCCTCGAC TTCTCCTGTGCCCTCTTTGGGCCAGATGGGGGCCTCGTCTCCAATGCTCCCCACATTCCTGTGCACCTGG GTGCCATGCAAGAGACTGTACAGTTCCAGATTCAGCACTTAGGAGCCGACCTCCATCCTGGTGATGTGTT GCTTAGTAACCATCCCAGTGCAGGGGGCAGCCATCTTCCTGACCTGACTGTCATCACACCGGTGTTTTGG CCAGGTCAGTCGAGGCCTGTGTTCTACGTGGCTAGCCGAGGGCACCACGCGGACATTGGAGGTATCACCC CGGGTTCTATGCCTCCTCACTCTACCACACTGCAGCAGGAGGGTGCCGTTTTTCTGTCCTTCAAACTGGT CCAGGGAGGAGTCTTCCAGGAAGAGGCGGTGACAGAGGCCTTACAGGCCCCAGGCAAGATCTCTGGCTGC AGTGGGACCAGGAACCTGCATGACAACCTGTCGGATCTCCGTGCCCAGGTGGCAGCTAACCAGAAAGGCA TCCAGCTGGTGGGAGAGCTGATCGGACAGTATGGCTTAGATGTGGTGCAGGCCTATATGGGCCATATTCA GGCAAATGCTGAACTAGCAGTGCGAGACATGCTTCGTGCTTTTGGAACTTCCCGCCAGGCCAGGGGCCTG CCCCTGGAGGTGTCTGCCAAGGATCACATGGACGATGGCTCTCCCATCTGCCTGCATGTTCAGATCAACC TGAATCAGGGCAGCGCTGTATTTGACTTCAGTGGTTCTGGGTCTGAGGTGTTTGGCAATCTCAATGCCCC TCGAGCCATAACACTGTCTGCTCTCATCTATTGCTTGCGCTGTCTAGTGGGCCGTGACATCCCACTTAAC CAGGGTTGCCTGGCTCCTGTGCAAGTCATAATTCCCAAAGGCTCCATATTGGATCCATCCCCAGAGGCAG CAGTGGTCGGAGGCAACGTGCTCACATCTCAGCGAGTCGTGGATGTCATCCTGGGGGCTTTTGGGGCCTG TGCAGCCTCCCAGGGCTGCATGAACAATGTGACCCTGGGCAATGCCCGTATGGGCTACTATGAGACAGTA GCTGGTGGTGCGGGTGCAGGCCCTGGCTGGCATGGGCGCAGTGGTGTACACAGTCACATGACCAACACGC GCATTACGGACCCAGAGATTTTGGAGAGCCGGTATCCAGTTATCCTGCGCCGCTTTGAGCTGAGGCCAGG CTCTGGAGGCCGAGGCCGCTTCCGGGGAGGTGATGGCGTAGTCCGAGAGCTGGTCTTTCGGGAAGAGGCG CTACTGTCTGTCCTCACGGAGCGCCGCGCCTTCCAGCCTTATGGCCTCCACGGGGGAGAGCCTGGTACAC GTGGCTTAAACCTCCTGATCAGAAAAGATGGGCGCACAGTGAATCTGGGCGGCAAGACATCCGTGACTGT GTATCCCGGGGACGCGTTCTGCCTCCACACGCCTGGGGGTGGGGGCTATGGAGACCCGGAGGACCCAGCA CCACCACCAGGCTCGCCCCCGCTATATCCAGCCTTCCCAGAGCGAGGCAGTGTGTATGAGTACCGCCGCG CCCAGGAAGCAGTA AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA >MR211894 representing NM_153122
Red=Cloning site Green=Tags(s) MGSPEERFHFAIDRGGTFTDVFAQCPGGHVRVLKLLSEDPANYADAPTEGIRRILEQERGVLLPRGRPLD TSHIASIRMGTTVATNALLERQGERVALLVTRGFRDLLHIGTQARPDLFDLAVPMPEVLYEEVVEVDERV LLYRGEPGAGSPVKGCTGDLLEIQQPVDLAALRGKLEGLLTRGIHSLAVVLMHSYTWAQHEQQVGTLARE LGFTHVSLSSEVMPMVRIVPRGHTACADAYLTPTIQRYVQGFRRGFQGQLKNVQVLFMRSDGGLAPMDAF SGSRAVLSGPAGGVVGYSTTTYQLEGGQPVIGFDMGGTSTDVSRYAGEFEHVFEASTAGVTLQAPQLDIN TVAAGGGSRLFFRSGLFVVGPESAGAHPGPACYRKGGPVTVTDANLVLGRLLPASFPCIFGPGEDQPLSP EASRKALEAVAMEVNSFLASGPCPASQLSLEEVAMGFVRVANEAMCRPIRALTQARGHDPSAHVLACFGG AGGQHACAIARALGMDTVHIHRHSGLLSALGLALADVVHEAQEPCSLSYTPETFAQLDQRLSRLEEQCVD ALQAQGFSRSQISTESFLHLRYQGTDCALMVSANQHPATTCSPRAGDFGAAFVERYMREFGFIIPERSVV VDDVRVRGTGRSGLQLEETSKIQSGPPHVEKVTQCYFEGGYQETPVYLLGELGYGHQLQGPCLIIDNNST ILVEPGCQAEVIETGDIRISVGAEAPSMIDTKLDPIQLSIFSHRFMSIAEQMGRILQRTAISTNIKERLD FSCALFGPDGGLVSNAPHIPVHLGAMQETVQFQIQHLGADLHPGDVLLSNHPSAGGSHLPDLTVITPVFW PGQSRPVFYVASRGHHADIGGITPGSMPPHSTTLQQEGAVFLSFKLVQGGVFQEEAVTEALQAPGKISGC SGTRNLHDNLSDLRAQVAANQKGIQLVGELIGQYGLDVVQAYMGHIQANAELAVRDMLRAFGTSRQARGL PLEVSAKDHMDDGSPICLHVQINLNQGSAVFDFSGSGSEVFGNLNAPRAITLSALIYCLRCLVGRDIPLN QGCLAPVQVIIPKGSILDPSPEAAVVGGNVLTSQRVVDVILGAFGACAASQGCMNNVTLGNARMGYYETV AGGAGAGPGWHGRSGVHSHMTNTRITDPEILESRYPVILRRFELRPGSGGRGRFRGGDGVVRELVFREEA LLSVLTERRAFQPYGLHGGEPGTRGLNLLIRKDGRTVNLGGKTSVTVYPGDAFCLHTPGGGGYGDPEDPA PPPGSPPLYPAFPERGSVYEYRRAQEAV SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_153122 |
| ORF Size | 3864 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_153122.3 |
| RefSeq Size | 3970 bp |
| RefSeq ORF | 3867 bp |
| Locus ID | 75475 |
| UniProt ID | Q8K010 |
| MW | 138.1 kDa |
| Gene Summary | Catalyzes the cleavage of 5-oxo-L-proline to form L-glutamate coupled to the hydrolysis of ATP to ADP and inorganic phosphate.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC224159 | Oplah (untagged) - Mouse 5-oxoprolinase (ATP-hydrolysing) (Oplah), (10ug) |
CNY 16,630.00 |
|
| MG211894 | Oplah (tGFP-tagged) - Mouse 5-oxoprolinase (ATP-hydrolysing) (Oplah) |
CNY 10,550.00 |
|
| MR211894L3 | Lenti ORF clone of Oplah (Myc-DDK-tagged) - Mouse 5-oxoprolinase (ATP-hydrolysing) (Oplah) |
CNY 11,500.00 |
|
| MR211894L4 | Lenti ORF clone of Oplah (mGFP-tagged) - Mouse 5-oxoprolinase (ATP-hydrolysing) (Oplah) |
CNY 11,500.00 |
