Kdm2b (NM_001003953) Mouse Tagged ORF Clone
CAT#: MR211909
- TrueORF®
Kdm2b (Myc-DDK-tagged) - Mouse lysine (K)-specific demethylase 2B (Kdm2b), transcript variant 1
"NM_001003953" in other vectors (2)
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CNY 13,020.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | Cxxc2; Fbl10; Fbxl1; Fbxl10; Jhdm1b; PCCX2 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR211909 representing NM_001003953
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGCAGAGAAAGACTCTGGAAGAAGATTGCGTGCGATTGACCGCCAGAGATACGACGAGAACGAAG ACTTGTCGGACGTGGAGGAAATTGTCAGCGTCCGTGGCTTCAGCCTGGAGGAGAAGCTACGTAGCCAGTT ATACCAGGGGGACTTCGTGCATGCTATGGAAGGCAAAGATTTTAACTATGAGTACGTACAGAGAGAAGCT CTCAGGGTCCCCCTGGTTTTTCGGGACAAGGATGGACTAGGGATCAAGATGCCAGACCCTGATTTCACAG TCCGAGACGTCAAACTCCTGGTGGGGAGCCGCCGTTTGGTGGATGTCATGGACGTCAACACCCAGAAGGG TACCGAGATGAGCATGTCCCAGTTTGTGCGCTACTACGAGACACCAGAGGCACAGCGGGATAAACTGTAC AACGTCATCAGCCTCGAGTTCAGCCATACGAAGCTGGAGCATCTGGTCAAGCGTCCCACTGTGGTGGACC TGGTCGACTGGGTGGACAACATGTGGCCTCAGCATCTAAAGGAAAAGCAGACAGAAGCCACCAATGCCCT TGCAGAGATGAAGTACCCCAAAGTGAAAAAGTACTGTCTGATGAGCGTGAAGGGCTGTTTCACTGACTTC CACATTGACTTTGGAGGCACCTCCGTGTGGTACCATGTGTTCCGTGGTGGCAAGATCTTTTGGCTGATCC CTCCAACCCTGCACAACTTGGCTTTGTACGAGGAGTGGGTGCTGTCTGGCAAACAGAGCGACATCTTTCT GGGAGACCGCGTGGAACGCTGCCAAAGAATTGAGCTGAAGCAAGGCTACACCTTTTTCATCCCTTCCGGT TGGATCCATGCGGTTTATACGCCTGTGGACTCTCTGGTGTTCGGCGGGAACATCCTGCATAGCTTCAACG TGCCCATGCAGCTGCGGATCTACGAGATCGAGGACAGGACCCGGGTTCAGCCCAAGTTCCGTTACCCCTT CTACTATGAGATGTGCTGGTATGTCTTGGAGAGATATGTGTACTGTGTGACCCAGCGCTCCTACCTCACT CAGGAATACCAGAGAGAATTAATGCTTATTGATGCCCCAAGAAAAACCAGTGTAGACGGCTTTTCATCCG ACTCCTGGCTGGACATGGAGGAGGAGTCCTGCGAGCAGCAGCCACAGGAGGAAGAGGAGGAGGAGGAGGA CAAGGAGGAGGAAGGAGATGGTGCAGATAAAACACCCAAGCCACCCACCGATGACCCCACCTCACCCACC AGCACCCCGCCCGAAGACCAGGACAGCACAGGGAAGAAGCCTAAAGCCCCTGCCATACGGTTCCTCAAGA GGACGTTGTCCAATGAGTCAGAGGAAAGTGTCAAGTCGACCTCGATGCCCACGGACGATCCCAAGACGCC CACGGGCTCCCCGGCCACCGAGGTTTCTACCAAGTGGACTCACCTTACCGAATTTGAACTGAAGGGCTTG AAAGCCCTGGTTGAAAAGCTAGAGTCCCTTCCGGAGAATAAGAAGTGTGTCCCTGAGGGAATCGAGGACC CCCAGGCCCTCCTGGAAGGTGTAAAGAATGTACTGAAAGAGCACGTGGATGACGACCCCACCCTGGCCAT CACCGGGGTCCCTGTGGTCAGCTGGCCAAAGAAAACTGCAAAGAACCGGGTGGTGGGTCGGCCTAAGGGC AAGTTGGGCCCGGCCTCAGCGGTGAAGTTGGCTGCCAACCGAACAACAGCAGGAGCTCGCAGGCGCCGGA CGCGATGCCGCAAGTGCGAGGCCTGCCTGCGGACGGAGTGTGGAGAGTGCCACTTTTGCAAGGACATGAA GAAGTTTGGAGGTCCTGGGCGCATGAAGCAGAGCTGCATCATGCGGCAGTGCATCGCGCCAGTGCTGCCC CACACCGCCGTGTGCCTTGTGTGTGGCGAGGCAGGGAAGGAGGACACAGTGGAAGAGGAAGAAGGCAAGT TTAACCTCATGCTCATGGAATGCTCCATCTGCAACGAGATCATCCACCCTGGATGCCTTAAGATTAAGGA ATCGGAGGGTGTGGTCAACGATGAGCTTCCCAACTGCTGGGAGTGTCCGAAGTGTAACCATGCCGGCAAG ACCGGGAAACAAAAGCGTGGCCCTGGCTTTAAGTATGCCTCCAACCTGCCTGGCTCCTTGCTCAAGGAGC AGAAGATGAACCGGGACAACAAGGAAGGGCAAGAGCCTGCCAAGCGGAGAAGTGAGTGTGAAGAGGCTCC CCGTCGGAGGTCAGACGAGCACCCCAAAAAGGTGCCTGCAGATGGCATCCTCCGCCGAAAGTCTGATGAT GTGCACCTGAGGAGGAAGCGGAAATACGAGAAGCCCCAAGAGCTGAGTGGACGCAAGCGAGCCTCGTCGC TTCAAACGTCCCCCGGTTCCTCCTCTCACCTCTCGCCGAGGCCCCCTCTAGGCAGCAGTCTCAGCCCTTG GTGGAGATCCAGTCTCACTTACTTCCAGCAGCAGCTAAAACCTGGCAAAGAAGATAAGCTTTTCAGGAAA AAGCGGCGGTCCTGGAAGAACGCTGAGGATCGTCTGTCACTGGCCAACAAGCCCCTTCGGCGCTTTAAGC AGGAACCGGAGGACGATCTGCCTGAGGCACCTCCTAAGACCCGGGAGAGTGATCAGTCACGTTCCAGCTC ACCCACTGCTGGTCCCAGCACTGAGGGAGCTGAAGGCCCAGAAGAGAAGAAAAAGGTGAAGATGCGCCGG AAGCGGCGACTTGTTAACAAGGAGCTGAGCAAAGAGCTAAGCAAGGAGCTCAACCATGAGATCCAAAAGA CGGAGAGCACCCTGGCTCACGAGAGCCAGCAGCCCATCAAGTCAGAGCCTGAGAGCGAGAACGACGAGCC CAAGAGGCCCTTAAGCCACTGCGAGCGCCCCCACCGCTTCAGCAAAGGGCTCAACGGCACACCTCGGGAG CTGCGGCACTCGCTGGGACCTGGCCTGCGTAGTCCACCTCGTGTTATGTCCCGGCCCCCGCCCTCTGCAT CCCCACCCAAGTGCATCCAGATGGAGCGTCACGTGATCCGGCCACCGCCCATCAGCCCCCCACCTGACTC GCTGCCCCTGGATGATGGAGCAGCCCACGTCATGCATAGGGAGGTGTGGATGGCAGTCTTCAGCTACCTC AGCCACCGAGACCTGTGTGTCTGCATGCGGGTCTGCAGGACCTGGAACCGCTGGTGCTGCGATAAGCGGT TGTGGACCCGCATCGACCTGAACCGCTGCAAGTCCATCACACCCCTGATGCTGAGCGGTATCATCCGGCG ACAGCCTGTCTCCCTTGACCTCAGTTGGACCAACATCTCCAAGAAGCAGCTGAGTTGGCTCATCAACCGG TTGCCTGGGCTCCGAGACTTGGTGCTGTCAGGCTGCTCATGGATCGCTGTCTCAGCCCTCTGTAGCTCCA GTTGTCCATTGCTCCGGACCCTGGATGTCCAGTGGGTAGAAGGACTAAAGGATGCCCAGATGAGGGATCT CCTGTCTCCACCCACAGACAACAGGCCAGGTCAGATGGACAATCGGAGCAAGCTCCGGAACATTGTAGAG CTGCGCCTAGCTGGCCTGGACATCACAGATGTCTCCCTGCGGCTCATTATTCGCCATATGCCCCTGCTCT CGAAGCTCCAACTCAGTTACTGTAACCACATCAATGACCAGTCCATCAACCTGCTCACTGCCGTCGGCAC CACCACCCGAGACTCGCTGACAGAGGTCAACCTATCAGACTGTAATAAGGTAACTGACCTGTGCCTGTCC TTCTTCAAACGCTGTGGAAATATCTGTCATATTGACCTGAGGTACTGCAAGCAAGTCACCAAGGAAGGCT GTGAGCAATTCATAGCTGAAATGTCTGTGAGTGTCCAATTTGGGCAAGTGGAAGAGAAACTCCTGCAAAA ACTAAGT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR211909 representing NM_001003953
Red=Cloning site Green=Tags(s) MEAEKDSGRRLRAIDRQRYDENEDLSDVEEIVSVRGFSLEEKLRSQLYQGDFVHAMEGKDFNYEYVQREA LRVPLVFRDKDGLGIKMPDPDFTVRDVKLLVGSRRLVDVMDVNTQKGTEMSMSQFVRYYETPEAQRDKLY NVISLEFSHTKLEHLVKRPTVVDLVDWVDNMWPQHLKEKQTEATNALAEMKYPKVKKYCLMSVKGCFTDF HIDFGGTSVWYHVFRGGKIFWLIPPTLHNLALYEEWVLSGKQSDIFLGDRVERCQRIELKQGYTFFIPSG WIHAVYTPVDSLVFGGNILHSFNVPMQLRIYEIEDRTRVQPKFRYPFYYEMCWYVLERYVYCVTQRSYLT QEYQRELMLIDAPRKTSVDGFSSDSWLDMEEESCEQQPQEEEEEEEDKEEEGDGADKTPKPPTDDPTSPT STPPEDQDSTGKKPKAPAIRFLKRTLSNESEESVKSTSMPTDDPKTPTGSPATEVSTKWTHLTEFELKGL KALVEKLESLPENKKCVPEGIEDPQALLEGVKNVLKEHVDDDPTLAITGVPVVSWPKKTAKNRVVGRPKG KLGPASAVKLAANRTTAGARRRRTRCRKCEACLRTECGECHFCKDMKKFGGPGRMKQSCIMRQCIAPVLP HTAVCLVCGEAGKEDTVEEEEGKFNLMLMECSICNEIIHPGCLKIKESEGVVNDELPNCWECPKCNHAGK TGKQKRGPGFKYASNLPGSLLKEQKMNRDNKEGQEPAKRRSECEEAPRRRSDEHPKKVPADGILRRKSDD VHLRRKRKYEKPQELSGRKRASSLQTSPGSSSHLSPRPPLGSSLSPWWRSSLTYFQQQLKPGKEDKLFRK KRRSWKNAEDRLSLANKPLRRFKQEPEDDLPEAPPKTRESDQSRSSSPTAGPSTEGAEGPEEKKKVKMRR KRRLVNKELSKELSKELNHEIQKTESTLAHESQQPIKSEPESENDEPKRPLSHCERPHRFSKGLNGTPRE LRHSLGPGLRSPPRVMSRPPPSASPPKCIQMERHVIRPPPISPPPDSLPLDDGAAHVMHREVWMAVFSYL SHRDLCVCMRVCRTWNRWCCDKRLWTRIDLNRCKSITPLMLSGIIRRQPVSLDLSWTNISKKQLSWLINR LPGLRDLVLSGCSWIAVSALCSSSCPLLRTLDVQWVEGLKDAQMRDLLSPPTDNRPGQMDNRSKLRNIVE LRLAGLDITDVSLRLIIRHMPLLSKLQLSYCNHINDQSINLLTAVGTTTRDSLTEVNLSDCNKVTDLCLS FFKRCGNICHIDLRYCKQVTKEGCEQFIAEMSVSVQFGQVEEKLLQKLS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001003953 |
ORF Size | 3927 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001003953.2 |
RefSeq Size | 5184 bp |
RefSeq ORF | 3930 bp |
Locus ID | 30841 |
UniProt ID | Q6P1G2 |
MW | 150.2 kDa |
Gene Summary | The protein encoded by this gene is a H3K36-specific histone demethylase, which contains an N-terminal jumonji C domain, a CxxC zinc finger domain, a plant homeodomain finger, an F-box, and eight leucine-rich repeats. Amongst its demonstrated functions, this protein plays roles in the suppression of premature cellular senescence, leukemia maintenance and development, maintenance of mouse embryonic stem cell pluripotency, and induced pluripotent stem cell generation. Mice homozygous for a targeted deletion of the zinc finger domain display embryonic lethality with development ceasing at approximately 7 to 8 days post coitum, demonstrating an essential role in early development. A pseudogene of this gene is found on chromosome 4. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2014] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MR211909L3 | Lenti ORF clone of Kdm2b (Myc-DDK-tagged) - Mouse lysine (K)-specific demethylase 2B (Kdm2b), transcript variant 1 |
CNY 14,920.00 |
|
MR211909L4 | Lenti ORF clone of Kdm2b (mGFP-tagged) - Mouse lysine (K)-specific demethylase 2B (Kdm2b), transcript variant 1 |
CNY 14,920.00 |