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Nup153 (NM_175749) Mouse Tagged ORF Clone
CAT＃: MR211997
- TrueORF®
Nup153 (Myc-DDK-tagged) - Mouse nucleoporin 153 (Nup153)
ORF Plasmid: tGFP
"NM_175749" in other vectors (4)
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CNY 15,008.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | B130015D15Rik; C88147 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR211997 representing NM_175749
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCTCGGAAGCCGGAGGCATAGGAGGGGGCGGCGGCGGCGGCAAGATCCGGACTCGTCGTTGCCACC AGGGGCCGGTGAAGCCTTACCAACAGGGGCGACCCCAACACCAGGGCATTCTTAGCAGGGTTACAGAATC TGTTAAGAATATTGTACCAGGGTGGCTACAAAGATACTTCAACAAGAGTGAAAATGCTTGCAGCTGTTCA CCAGATGCAGATGAAGTTCCACCCTGGCCAGAAAATAGAGAAGATGAGCATGCGATTTATGCGGATGAGA ACACAAACACTGATGATGGGAGAATCACCCCTGACCCTGCAGGCAGTAACACAGAAGAACCATCAACAAC CAGTACTGCTTCAAATTATCCAGATGTGTTAACAAGACCTTCTCTTCATCGGAGTCATCTGAATTTCTCT GTGTTGGAATCCCCTGCTTTGCACTGTCAGCCGTCCACATCCTCTGCGTTCCCAATCGGCAGTTCTGGAT TTTCCCTTGTGAAGGAGATTAAAGATTCTACCTCTCAGCATGATGATGATAACATCTCAACTACCAGTGG GTTCTCTTCAAGAGCTTCTGAGAAAGATATAGCTGTTTCAAAGAACACTTCCCTGCCACCTTTGTGGTCC CCTGAAGCAGAACGTTCTCATTCACTCTCACAGCATACTGCCATCAGCTCCAAAAAACCAGCATTCAACT TGTCTGCCTTTGGAACACTTTCCACCTCACTTGGGAATTCTTCAATTCTTAAAACAAGTCAGCTTGGAGA CTCTCCTTTTTATCCTGGCAAAACAACATATGGAGGGGCAGCTGCTGCTGTAAGACAGAATAAAGTACGA AGTACACCTTACCAGGCTCCAGTTAGAAGACAGATGAAAGCTAAGCAGCTCAATGCACAGTCTTATGGTG TGACCAGCTCAACAGCACGGCGGATACTGCAGTCATTAGAAAAGATGTCGAGCCCTCTTGCGGATGCAAA AAGAATTCCATCTGCAGTTTCTTCTCCTCTGAATTCTCCTCTTGATAGGAGTGGGATAGATAACACAGTT TTTCAGGCAAAAAAAGAAAAGGTGGACTCTCAGTATCCCCCTGTTCAGAGACTAATGACCCCAAAGCCAG TTTCCATAGCAACAAATAGAACTGTCTATTTTAAACCATCTCTGACCCCATCTGGTGACTTAAGGAAGAC TAATCAAAGGATAGATAAAAAGAACAGTACTGTAGATGAAAAAAGTATCTCAAGACAAAATAGAGAGCAA GAAAGTGGCTTTTCATACCCCAATTTCAGTATTCCTGCAGCCAATGGTTTATCTTCTGGAGTAGGTGGTG GAGGTGGCAAGATGAGAAGAGAGAGAACACATTTTGTTGCTCCTAAACCTCCAGAGAATGAGGAAGTGGA AGCACCATTGTTACCGCAAATCTCTCTACCAATCAGCAGTTCTTCACTGCCCACCTTCAGTTTTAGTTCC CCTGTGACCTCGGCTTCTCCTTCACCAGTCAGCTCTTCTCAGCCATTACCAAACAAGGTACAGATGACCT CTCTGGGCAGCACGGGCAGTCCTGTGTTCACATTCTCATCTCCCATTGTAAAATCCACGCAGGCAGCTGT GCTCCCTCCAGCATCTATTGGATTTACATTTAGTGTGCCTCTTGCAAAAACTGAATTTTCTGGCTCTAAT AGTTCTTCAGAAACAGTTTTAAGTAGTTCAGCCCAAGATATCACTGCAGTGAATAGTTCAAGCTATAAGA AGCGAAGTGCTCCTTGTGAGGACCCCTTTACACCTGCAAAGATCCTGAGAGAAGGAAGTGTTCTAGACAT TCTGAAAACACCTGGTTTCGCATCACCAAAGGTTGATTCTCCTGCTCTTCAACCCACTACCACAAGCTCA ATAGTTTATACAAGACCAGCAATAAGTACTTTTTCTTCTAGTGGAATTGAGTATGGAGAAAGTTTAAAAG CTGGATCATCATGGCAGTGTGACACATGTCTCCTCCAGAACAAAGTCACAGACAATAAGTGTATAGCCTG CCAAGCAGCAAAACTGCCCCTGAAAGAAACTGCTAAACAGACTGGGACTGGGACACCAAGCAAAAGTGAC AAACCAGCCTCTACATCAGGGACAGGCTTTGGAGATAAATTTAAACCAGCAATAGGAACTTGGGATTGTG ATACCTGTTTAGTGCAAAATAAGCCCGAAGCAGTAAAATGTGTAGCCTGTGAGACACCTAAGCCTGGCAC TGGTGTGAAGCGAGCCCTTACATTGACAGTGGCTTCAGAAAGTCCTGTGACTGCTTCCTCTTCTACCACT GTGACAACTGGTACACTAGGATTTGGAGATAAATTCAAAAGGCCTGTGGGCTCTTGGGAGTGTCCAGTCT GCTGTGTTTGTAATAAGGCAGAAGACAACAGATGTGTTTCCTGTACATCTGAGAAACCAGGTTTGGTATC TGCCTCAAGCAGCAGCCCTGCCCCTGTCTCTCTGTCTTCTGGAGGCTGTTTAGGATTGGACAAGTTTAAA AAGCCCGAGGGAAGCTGGGACTGTGAAGTGTGCCTAGTGCAAAATAAAGCAGACTCTGCCAAGTGCATAG CTTGTGAAAGTGCAAAGCCAGGCACAAAGTCTGAGTTTAAAGGCTTCGGGACATCTTCATCTTTAAATCC AGCACCCTCAGCCTTCAAATTTGGTATTCCATCGTCCTCTTCTGGGCTTTCTCAAACTTTGACAAGCACT GGAAATTTTAAATTTGGAGATCAGGGAGGATTCAAATTAGGCACTTCATCTGACTCTGGGTCTACAAACA CCATGAATACAAACTTTAAATTTTCTAAACCAACTGGAGACTTTAAATTTGGAGTGTTATCTGATTCTAA ACCTGAAGAAGTTAAAAACGACAACAAGAATGATAATTTTCAGTTTGGATCTTCTTCTGGCTTAACCAAC CCAGCTTCTTCAGCTCCGTTTCAGTTTGGGGTATCTACTCTTGGGCAACAAGAAAAGAAAGAGGAGCTGC CTAAATCTTCACCTGCAGGCTTTAGCTTTGGTGCAGGTGTTAATAACCCCCCTAATGCTGCTATTGACAC CACAGCGACCTCAGAGAACAAGAGCGGCTTCAACTTTGGAACCCTAGACACAAAGAGTGTCTCAGTGACT CCTTTCACATACAAGACAACAGAAGCAAAGAAAGAAGACGCACCTGCTACCAAGGGTGGGTTCACATTTG GTAAAGTGGGCTCCTCTTCTTTGCCATCTTCCTCAATGTTTGTTTTGGGAAGGACAGAGGAGAAACAGCA AGAACCTGTTACTTCTACTTCTCTGGTGTTTGGGAAGAAAGCCGACAGTGAGGAGCCAAAGTGTCAGCCA GTATTTTCCTTTGGGAATTCAGAGCAAACCAAAGATGAGAGCTCCAAGCCGACATTCAGCTTCAGTGTGG CAAAACCATCTGGGAAGGAGTCTGAGCAGCTAGCCAAGGCTACTTTTGCATTTGGAAATCAAACCAATAC AACAACTGATCAAGGTGCAGCAAAGCCAGTTTTTAGCTTCTTGAACAGCAGTTCCTCTAGTTCAAGTGCA CCAGCCACTTCATCTAGTGGTGGCATATTTGGTAGTTCCACCTCTTCCTCCAACCCACCTGTGGCAGCCT TTGTGTTTGGACAGGCCAGCAATCCTGTCAGCAGCTCTGCCTTCGGTAACGCTGCTGAATCCAGTACATC TCAGTCTTTGCTATTTCCTCAAGAGAGCGAGCCAGCCACCACGTCCAGCACTGCCCCAGCTGCCTCTCCC TTTGTATTTGGGACAGGAGCCAGCAGTAATAGTGTGTCTTCTGGGTTCACTTTTGGGGCTACAACCACGT CAAGCTCTTCAGGATCCTCCTTTGTATTTGGCACTGGACATTCAGCACCATCTGCCAGTCCAGCATTTGG TGCTAACCAGACTCCAACATTTGGACAAAGTCAAGGTGCCAGCCAGCCTAACCCGCCAAGCTTTGGCTCT ATCTCGTCTTCAACAGCATTATTTTCTGCTGGTTCTCAGCCTGTACCACCACCTATATTTGGGACAGTGT CAAGCAGCAGCCAACCTCCTGTGTTTGGACAGCAGCCTAGTCAGTCTGCATTTGGCTCTGGGACAGCTAA TGCCAGTTCGGTTTTCCAATTTGGTAGCAGCACTACGAATTTCAACTTCACAAACAATAATCCATCAGGA GTGTTCACATTTGGTGCAAGTCCCAGCACGCCTGCAGCCTCAGCCCAGCCCTCAGGTTCAGGGGTCTTTT CATTCAGCCAGTCTCCAGCATCATTTACAGTGGGGTCAAATGGGAAAAATATGTTCTCTTCTTCCGGAAC TTCAGTTTCTGGTCGCAAGATAAAGACTGCTGTTAGACGCAAGAAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR211997 representing NM_175749
Red=Cloning site Green=Tags(s) MASEAGGIGGGGGGGKIRTRRCHQGPVKPYQQGRPQHQGILSRVTESVKNIVPGWLQRYFNKSENACSCS PDADEVPPWPENREDEHAIYADENTNTDDGRITPDPAGSNTEEPSTTSTASNYPDVLTRPSLHRSHLNFS VLESPALHCQPSTSSAFPIGSSGFSLVKEIKDSTSQHDDDNISTTSGFSSRASEKDIAVSKNTSLPPLWS PEAERSHSLSQHTAISSKKPAFNLSAFGTLSTSLGNSSILKTSQLGDSPFYPGKTTYGGAAAAVRQNKVR STPYQAPVRRQMKAKQLNAQSYGVTSSTARRILQSLEKMSSPLADAKRIPSAVSSPLNSPLDRSGIDNTV FQAKKEKVDSQYPPVQRLMTPKPVSIATNRTVYFKPSLTPSGDLRKTNQRIDKKNSTVDEKSISRQNREQ ESGFSYPNFSIPAANGLSSGVGGGGGKMRRERTHFVAPKPPENEEVEAPLLPQISLPISSSSLPTFSFSS PVTSASPSPVSSSQPLPNKVQMTSLGSTGSPVFTFSSPIVKSTQAAVLPPASIGFTFSVPLAKTEFSGSN SSSETVLSSSAQDITAVNSSSYKKRSAPCEDPFTPAKILREGSVLDILKTPGFASPKVDSPALQPTTTSS IVYTRPAISTFSSSGIEYGESLKAGSSWQCDTCLLQNKVTDNKCIACQAAKLPLKETAKQTGTGTPSKSD KPASTSGTGFGDKFKPAIGTWDCDTCLVQNKPEAVKCVACETPKPGTGVKRALTLTVASESPVTASSSTT VTTGTLGFGDKFKRPVGSWECPVCCVCNKAEDNRCVSCTSEKPGLVSASSSSPAPVSLSSGGCLGLDKFK KPEGSWDCEVCLVQNKADSAKCIACESAKPGTKSEFKGFGTSSSLNPAPSAFKFGIPSSSSGLSQTLTST GNFKFGDQGGFKLGTSSDSGSTNTMNTNFKFSKPTGDFKFGVLSDSKPEEVKNDNKNDNFQFGSSSGLTN PASSAPFQFGVSTLGQQEKKEELPKSSPAGFSFGAGVNNPPNAAIDTTATSENKSGFNFGTLDTKSVSVT PFTYKTTEAKKEDAPATKGGFTFGKVGSSSLPSSSMFVLGRTEEKQQEPVTSTSLVFGKKADSEEPKCQP VFSFGNSEQTKDESSKPTFSFSVAKPSGKESEQLAKATFAFGNQTNTTTDQGAAKPVFSFLNSSSSSSSA PATSSSGGIFGSSTSSSNPPVAAFVFGQASNPVSSSAFGNAAESSTSQSLLFPQESEPATTSSTAPAASP FVFGTGASSNSVSSGFTFGATTTSSSSGSSFVFGTGHSAPSASPAFGANQTPTFGQSQGASQPNPPSFGS ISSSTALFSAGSQPVPPPIFGTVSSSSQPPVFGQQPSQSAFGSGTANASSVFQFGSSTTNFNFTNNNPSG VFTFGASPSTPAASAQPSGSGVFSFSQSPASFTVGSNGKNMFSSSGTSVSGRKIKTAVRRKK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_175749 |
| ORF Size | 4386 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_175749.2, NP_786925.2 |
| RefSeq Size | 6021 bp |
| RefSeq ORF | 4389 bp |
| Locus ID | 218210 |
| MW | 152.5 kDa |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC204716 | Nup153 (untagged) - Mouse nucleoporin 153 (Nup153), (10ug) |
CNY 15,016.00 |
|
| MG211997 | Nup153 (tGFP-tagged) - Mouse nucleoporin 153 (Nup153) |
CNY 16,608.00 |
|
| MR211997L3 | Lenti ORF clone of Nup153 (Myc-DDK-tagged) - Mouse nucleoporin 153 (Nup153) |
CNY 16,530.00 |
|
| MR211997L4 | Lenti ORF clone of Nup153 (mGFP-tagged) - Mouse nucleoporin 153 (Nup153) |
CNY 17,408.00 |
