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Col4a1 (BC072650) Mouse Tagged ORF Clone
CAT＃: MR212033
- TrueORF®
Col4a1 (Myc-DDK-tagged) - Mouse procollagen, type IV, alpha 1 (cDNA clone MGC:91373 IMAGE:6852661)
ORF Plasmid: tGFP
"BC072650" in other vectors (4)
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CNY 15,580.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Bru; Col4a-1; Raw; Svc |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR212033 representing BC072650
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGCCCCGGCTCAGCGTCTGGCTTCTGCTGCTCTTCGCCGCCCTTCTGCTCCACGAGGAGCGCAGCC GAGCAGCTGCGAAGGGCGATTGTGGTGGCTCTGGCTGTGGAAAATGTGACTGTCATGGCGTGAAGGGACA AAAGGGAGAAAGAGGCTTGCCAGGGTTGCAAGGTGTCATTGGATTTCCCGGGATGCAAGGACCTGAGGGG CCACATGGACCACCAGGACAAAAGGGTGATGCTGGAGAACCTGGACTTCCTGGCACAAAAGGGACGAGGG GACCCCCTGGAGCAGCTGGCTACCCTGGGAATCCCGGACTTCCTGGTATTCCTGGCCAAGATGGCCCTCC AGGTCCCCCAGGTATCCCAGGATGCAACGGTACAAAGGGAGAGAGAGGGCCGCTCGGTCCTCCTGGCTTG CCTGGATTCAGCGGCAATCCTGGACCACCAGGGTTACCAGGAATGAAGGGAGATCCTGGTGAAATTCTCG GCCACGTTCCCGGAACACTGCTGAAAGGGGAGAGAGGATTTCCTGGTATCCCCGGGATGCCGGGCTCACC AGGGTTGCCAGGACTCCAGGGACCCGTGGGTCCTCCGGGATTTACTGGACCACCGGGTCCTCCAGGCCCT CCTGGACCTCCTGGAGAAAAGGGCCAGATGGGCTCCAGCTTCCAAGGACCAAAAGGTGACAAGGGAGAGC AAGGGGTCAGCGGTCCCCCGGGAGTTCCTGGACAGGCACAAGTTAAGGAAAAAGGAGACTTTGCCCCAAC AGGAGAAAAGGGTCAGAAAGGTGAACCTGGATTTCCGGGAGTACCAGGATATGGAGAGAAAGGTGAACCT GGCAAGCAAGGGCCTCGGGGAAAACCTGGGAAAGACGGTGAAAAAGGAGAAAGGGGGAGCCCGGGCATTC CTGGCGATTCCGGGTACCCAGGTCTCCCAGGCCGGCAGGGCCCGCAGGGAGAGAAGGGTGAAGCTGGACT TCCAGGCCCCCCTGGAACTGTGATAGGCACCATGCCTTTGGGAGAGAAAGGAGATCGAGGCTACCCAGGA GCACCAGGGTTGAGAGGAGAGCCCGGCCCTAAAGGTTTCCCTGGAACACCAGGCCAACCAGGCCCTCCAG GTTTCCCTACTCCAGGCCAGGCTGGTGCTCCAGGCTTCCCAGGTGAAAGGGGAGAAAAAGGTGACCAGGG ATTTCCAGGCGTGTCATTGCCAGGACCAAGTGGAAGAGATGGAGCCCCGGGGCCTCCCGGCCCTCCCGGC CCCCCTGGGCAGCCAGGCCACACAAATGGCATTGTGGAGTGTCAACCTGGACCACCCGGGGACCAGGGCC CTCCTGGGACTCCAGGACAGCCAGGTTTGACAGGTGAAGTTGGACAGAAAGGTCAGAAAGGAGAGAGCTG CCTTGCCTGTGACACAGAAGGACTTCGTGGCCCTCCAGGGCCACAGGGGCCTCCGGGAGAGATTGGTTTC CCTGGACAACCTGGGGCTAAAGGTGACCGAGGCCTGCCCGGCAGAGATGGTCTTGAAGGATTGCCGGGTC CACAAGGTTCACCAGGGCTTATAGGCCAGCCTGGAGCTAAGGGAGAGCCTGGAGAGATATTTTTTGACAT GCGACTCAAAGGTGACAAAGGAGACCCAGGTTTTCCGGGACAGCCTGGCATGCCAGGAAGAGCAGGAACT CCCGGAAGAGATGGCCACCCAGGACTCCCTGGACCCAAAGGCTCTCCGGGTTCAATAGGATTGAAAGGAG AGCGAGGTCCTCCCGGAGGAGTTGGCTTCCCTGGTAGTCGTGGAGACATCGGCCCTCCTGGACCCCCAGG AGTTGGCCCCATTGGTCCTGTTGGTGAAAAAGGACAAGCAGGCTTTCCTGGAGGCCCTGGGTCCCCAGGT CTTCCAGGCCCAAAGGGTGAGGCAGGAAAGGTTGTCCCACTCCCTGGCCCCCCAGGAGCTGCAGGACTTC CAGGATCCCCTGGCTTCCCAGGGCCACAAGGTGACCGAGGCTTCCCAGGAACCCCAGGACGTCCAGGCAT CCCGGGAGAGAAAGGTGCTGTGGGCCAGCCAGGAATTGGATTTCCTGGGCTTCCTGGCCCCAAAGGTGTT GATGGCTTGCCTGGAGAGATAGGACGGCCTGGGAGTCCAGGTCGCCCTGGATTTAACGGCTTACCTGGCA ACCCAGGACCTCAAGGTCAAAAGGGAGAACCTGGCATTGGGCTTCCAGGACTCAAAGGGCAACCAGGCCT TCCAGGCATTCCCGGTACACCTGGAGAGAAGGGTAGCATCGGGGGACCCGGCGTTCCAGGAGAACAGGGG TTGACAGGCCCCCCAGGACTCCAGGGGATCAGAGGTGACCCAGGGCCTCCTGGAGTTCAAGGCCCAGCAG GTCCACCAGGGGTCCCAGGAATAGGGCCACCCGGAGCTATGGGCCCTCCCGGAGGGCAAGGACCACCAGG GTCATCAGGTCCACCTGGAATTAAAGGAGAGAAAGGGTTCCCTGGATTCCCCGGACTGGATATGCCTGGC CCCAAAGGCGATAAAGGCTCTCAAGGACTTCCTGGCCTCACAGGACAGTCAGGCCTCCCTGGCCTTCCTG GACAGCAGGGGACACCTGGAGTTCCAGGGTTCCCAGGTTCTAAAGGTGAAATGGGTGTCATGGGAACCCC GGGACAACCAGGCTCGCCAGGACCAGCAGGCACCCCAGGGTTACCTGGAGAAAAAGGGGACCATGGCCTT CCGGGCTCCTCAGGACCCAGGGGCGACCCTGGCTTCAAAGGTGATAAAGGTGACGTTGGGCTTCCTGGCA TGCCAGGATCCATGGAGCATGTGGACATGGGGAGCATGAAGGGACAGAAAGGAGACCAGGGAGAGAAAGG ACAAATCGGACCCACTGGTGATAAAGGTTCCCGAGGAGACCCTGGAACACCAGGAGTACCTGGGAAGGAT GGGCAGGCAGGGCATCCCGGACAGCCAGGGCCTAAAGGTGACCCAGGCCTTAGTGGGACACCAGGATCCC CTGGACTCCCTGGACCCAAAGGATCAGTTGGAGGAATGGGCTTGCCAGGTTCGCCTGGAGAAAAAGGTGT GCCTGGCATCCCTGGCTCACAGGGTGTCCCTGGCTCACCTGGAGAGAAGGGAGCCAAAGGAGAGAAAGGG CAGTCAGGTCTACCTGGCATTGGGATTCCGGGACGGCCTGGTGACAAGGGAGACCAGGGCCTTGCTGGCT TCCCAGGCAGCCCCGGTGAGAAGGGAGAGAAAGGCAGTGCCGGAACCCCAGGGATGCCAGGGTCCCCAGG CCCGAGAGGTTCTCCGGGGAACATCGGCCATCCAGGAAGCCCAGGCCTGCCTGGAGAGAAAGGGGATAAA GGCCTCCCAGGACTGGATGGCGTTCCTGGTGTCAAAGGAGAAGCAGGTCTCCCTGGGACTCCTGGCCCCA CAGGCCCAGCTGGCCAGAAGGGAGAGCCGGGCAGCGATGGAATCCCGGGGTCGGCAGGAGAGAAGGGTGA ACAAGGTGTTCCAGGAAGAGGCTTCCCCGGCTTCCCTGGCTCCAAAGGAGACAAAGGCTCCAAGGGTGAA GTGGGTTTCCCTGGCCTAGCTGGAAGTCCTGGAATTCCTGGAGTCAAAGGCGAGCAAGGGTTCATGGGTC CTCCTGGCCCTCAAGGACAACCTGGCTTACCTGGCACTCCTGGTCACCCTGTGGAGGGGCCCAAAGGAGA CCGAGGACCTCAGGGTCAACCTGGCCTGCCAGGGCATCCGGGACCTATGGGGCCGCCAGGGTTCCCAGGA ATCAATGGGCCAAAAGGTGACAAGGGAAATCAAGGTTGGCCAGGAGCTCCGGGGGTTCCAGGCCCTAAGG GAGACCCAGGATTCCAAGGCATGCCGGGCATTGGCGGCTCTCCAGGGATCACAGGTTCAAAGGGAGATAT GGGACTGCCCGGAGTTCCAGGATTTCAAGGTCAGAAAGGGCTTCCTGGTCTGCAGGGAGTGAAAGGAGAC CAGGGAGACCAAGGTGTACCCGGCCCTAAAGGTCTCCAAGGTCCCCCTGGGCCTCCAGGTCCCTACGATG TCATCAAAGGAGAACCAGGGCTCCCTGGTCCTGAGGGTCCCCCTGGTCTTAAAGGACTCCAGGGACCACC AGGTCCAAAAGGACAGCAAGGTGTGACAGGCTCAGTGGGCTTGCCTGGACCTCCAGGTGTCCCTGGGTTC GATGGTGCTCCTGGCCAGAAAGGAGAGACAGGACCCTTTGGACCACCTGGTCCAAGAGGGTTTCCTGGCC CACCAGGCCCCGATGGGCTGCCAGGATCCATGGGTCCCCCAGGTACCCCATCTGTGGACCATGGCTTCCT TGTGACCAGGCATAGTCAGACAACAGATGACCCACTGTGTCCCCCAGGGACCAAAATTCTTTACCATGGA TACTCTCTGCTCTATGTCCAAGGCAACGAGCGTGCCCACGGGCAGGACTTGGGTACGGCTGGCAGCTGCC TGCGTAAGTTCAGCACCATGCCCTTTCTCTTCTGCAACATCAACAACGTCTGCAACTTCGCCTCCAGGAA CGACTACTCTTACTGGCTGTCCACGCCAGAGCCCATGCCCATGTCCATGGCACCCATCTCTGGGGACAAC ATCCGGCCCTTCATTAGCAGCTTTCCTTGGAAAACAGGTGTGCGGTTTGTGAAGCACCGGCCATGG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR212033 representing BC072650
Red=Cloning site Green=Tags(s) MGPRLSVWLLLLFAALLLHEERSRAAAKGDCGGSGCGKCDCHGVKGQKGERGLPGLQGVIGFPGMQGPEG PHGPPGQKGDAGEPGLPGTKGTRGPPGAAGYPGNPGLPGIPGQDGPPGPPGIPGCNGTKGERGPLGPPGL PGFSGNPGPPGLPGMKGDPGEILGHVPGTLLKGERGFPGIPGMPGSPGLPGLQGPVGPPGFTGPPGPPGP PGPPGEKGQMGSSFQGPKGDKGEQGVSGPPGVPGQAQVKEKGDFAPTGEKGQKGEPGFPGVPGYGEKGEP GKQGPRGKPGKDGEKGERGSPGIPGDSGYPGLPGRQGPQGEKGEAGLPGPPGTVIGTMPLGEKGDRGYPG APGLRGEPGPKGFPGTPGQPGPPGFPTPGQAGAPGFPGERGEKGDQGFPGVSLPGPSGRDGAPGPPGPPG PPGQPGHTNGIVECQPGPPGDQGPPGTPGQPGLTGEVGQKGQKGESCLACDTEGLRGPPGPQGPPGEIGF PGQPGAKGDRGLPGRDGLEGLPGPQGSPGLIGQPGAKGEPGEIFFDMRLKGDKGDPGFPGQPGMPGRAGT PGRDGHPGLPGPKGSPGSIGLKGERGPPGGVGFPGSRGDIGPPGPPGVGPIGPVGEKGQAGFPGGPGSPG LPGPKGEAGKVVPLPGPPGAAGLPGSPGFPGPQGDRGFPGTPGRPGIPGEKGAVGQPGIGFPGLPGPKGV DGLPGEIGRPGSPGRPGFNGLPGNPGPQGQKGEPGIGLPGLKGQPGLPGIPGTPGEKGSIGGPGVPGEQG LTGPPGLQGIRGDPGPPGVQGPAGPPGVPGIGPPGAMGPPGGQGPPGSSGPPGIKGEKGFPGFPGLDMPG PKGDKGSQGLPGLTGQSGLPGLPGQQGTPGVPGFPGSKGEMGVMGTPGQPGSPGPAGTPGLPGEKGDHGL PGSSGPRGDPGFKGDKGDVGLPGMPGSMEHVDMGSMKGQKGDQGEKGQIGPTGDKGSRGDPGTPGVPGKD GQAGHPGQPGPKGDPGLSGTPGSPGLPGPKGSVGGMGLPGSPGEKGVPGIPGSQGVPGSPGEKGAKGEKG QSGLPGIGIPGRPGDKGDQGLAGFPGSPGEKGEKGSAGTPGMPGSPGPRGSPGNIGHPGSPGLPGEKGDK GLPGLDGVPGVKGEAGLPGTPGPTGPAGQKGEPGSDGIPGSAGEKGEQGVPGRGFPGFPGSKGDKGSKGE VGFPGLAGSPGIPGVKGEQGFMGPPGPQGQPGLPGTPGHPVEGPKGDRGPQGQPGLPGHPGPMGPPGFPG INGPKGDKGNQGWPGAPGVPGPKGDPGFQGMPGIGGSPGITGSKGDMGLPGVPGFQGQKGLPGLQGVKGD QGDQGVPGPKGLQGPPGPPGPYDVIKGEPGLPGPEGPPGLKGLQGPPGPKGQQGVTGSVGLPGPPGVPGF DGAPGQKGETGPFGPPGPRGFPGPPGPDGLPGSMGPPGTPSVDHGFLVTRHSQTTDDPLCPPGTKILYHG YSLLYVQGNERAHGQDLGTAGSCLRKFSTMPFLFCNINNVCNFASRNDYSYWLSTPEPMPMSMAPISGDN IRPFISSFPWKTGVRFVKHRPW TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC072650 |
| ORF Size | 4686 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC072650.1 |
| RefSeq Size | 6522 bp |
| RefSeq ORF | 4688 bp |
| Locus ID | 12826 |
| MW | 239.1 kDa |
| Gene Summary | This gene encodes the alpha-1 subunit of the type IV collagens, an essential component of basement membranes. The encoded protein forms a triple helical heterotrimer comprised of two alpha-1 and one alpha-2 subunits that assembles into a type IV collagen network. This gene is located adjacent to the gene encoding alpha-2 subunit. Mice lacking both the alpha-1 and alpha-2 subunits of collagen IV die in utero due to structural deficiencies in the basement membranes and certain mutations in this gene cause perinatal cerebral hemorrhage and porencephaly. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Nov 2015] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC224566 | Col4a1 (untagged) - Mouse procollagen, type IV, alpha 1 (cDNA clone MGC:91373 IMAGE:6852661), (10ug) |
CNY 19,190.00 |
|
| MG212033 | Col4a1 (tGFP-tagged) - Mouse procollagen, type IV, alpha 1 (cDNA clone MGC:91373 IMAGE:6852661) |
CNY 17,100.00 |
|
| MR212033L3 | Lenti ORF clone of Col4a1 (Myc-DDK-tagged) - Mouse procollagen, type IV, alpha 1 (cDNA clone MGC:91373 IMAGE:6852661) |
CNY 17,480.00 |
|
| MR212033L4 | Lenti ORF clone of Col4a1 (mGFP-tagged) - Mouse procollagen, type IV, alpha 1 (cDNA clone MGC:91373 IMAGE:6852661) |
CNY 17,480.00 |
